Validation of a LC-UV method for determination of tildipirosin in goat milk

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Published: Jan 21, 2023
DOI: https://doi.org/10.12681/jhvms.28300
Keywords:
Goats HPLC Milk Tildipirosin Ultraviolet.
JS Galecio
Universidad San Francisco de Quito. EC 170157. Cumbayá, Ecuador
https://orcid.org/0000-0002-5532-9888
V Hernandis
1Department of Pharmacology, Faculty of Veterinary Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain.
https://orcid.org/0000-0002-6412-9423
E Escudero
University of Murcia
https://orcid.org/0000-0002-4354-322X
P Marín
University of Murcia
https://orcid.org/0000-0003-4849-4452
Abstract

A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in milk goat. Milk samples were precipitated with acetonitrile, and after evaporation, tildipirosin was determined by reverse-phase chromatography with an ultraviolet detector set at a wavelength of 289 nm. Tildipirosin was separated on a Zorbax Eclipse XDB-C18 column, 150 x 3.0 mm, 5 μm with gradient chromatographic elution. The retention times for tildipirosin and tylosin tartrate were 4.4 min and 10.5 min, respectively. Calibration curves were ranged from 100 to 2500 µg/L. The lower limit of detection was 75 µg/L, and the lower limit of quantitation was 100 µg/L. The accuracy and precision were always <15% except for LOQ < 20%. Mean recovery was 95.6 %. This procedure can be applied to perform pharmacokinetic studies in lactating animals and be useful to detect residues in dairy products.

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Author Biographies
JS Galecio, Universidad San Francisco de Quito. EC 170157. Cumbayá, Ecuador

Escuela de Medicina Veterinaria. Colegio de Ciencias de la Salud.

E Escudero, University of Murcia

Department of Pharmacology.

Faculty of Veterinary Medicine

P Marín, University of Murcia

Department of Pharmacology.

Faculty of Veterinary Medicine

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