Association of the caprine calpastatin MspI polymorphism with growth and reproduction traits in Saanen goats
Keywords:
Saanen birth weight CAST single nucleotide polymorphism
Abstract
This study was designed to evaluate the effects of calpastatin (CAST) MspI polymorphism on some growth and reproduction traits, including birth weight, first breeding weight, litter size, and average daily weight gain in Saanen goats. In this sense, blood samples obtained from 73 purebred female Saanen goats were used for genotyping. Genomic DNA was isolated by the phenol-chloroform method and used to determine CAST genotypes, including MM, MN, and NN, by means of the PCR-RFLP method. The population genetic parameters were estimated based on allelic distribution and the data were statistically analysed using analysis of variance (ANOVA) using a general linear model (GLM). Results revealed that N allele frequency was remarkably high (0.64) and the MM genotype was not present. The frequency of the heterozygous genotype was 59.62%. Concerning ANOVA results, significant differences were found between genotypes of the CAST locus concerning birth weight (P<0.05). In this respect, animals with the NN genotype were associated with higher birth weight means (2.85±0.29 kg) compared to heterozygous animals (2.53±0.24 kg). There was no significant association between the CAST marker and any of the remaining phenotypic traits evaluated. The present results suggest that focusing on this genomic region may be particularly useful in improving birth weight in goats which can be considered as an early indicator of post-weaning animal growth and survival.
Article Details
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ARDICLI, S., USTUNER, H., & ARSLAN, O. (2021). Association of the caprine calpastatin MspI polymorphism with growth and reproduction traits in Saanen goats. Journal of the Hellenic Veterinary Medical Society, 72(3), 3023–3030. https://doi.org/10.12681/jhvms.28483
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- Vol. 72 No. 3 (2021)
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- Research Articles
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References
Ardicli S, Samli H, Vatansever B, Soyudal B, Dincel D, Balci F (2019) Comprehensive assessment of candidate genes associated with fattening performance in Holstein-Friesian bulls. Arch Anim Breed 62:9-32.
Awemu E, Nwakalor L, Abubakar B (1999) Environmental influences on preweaning mortality and reproductive performance of Red Sokoto does. Small Ruminant Res 34: 161-165.
Bolormaa S, Pryce JE, Reverter A, Zhang Y, Barendse W, Kemper K, Tier B, Savin K, Hayes BJ, Goddard M (2014) A multi-trait, meta-analysis for detecting pleiotropic polymorphisms for stature, fatness and reproduction in beef cattle. PLoS Genet. 10(3):e1004198.
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32: 314-331.
Byun S, Zhou H, Forrest R, Frampton C, Hickford J (2008) Association of the ovine calpastatin gene with birth weight and growth rate to weaning. Anim Genet39: 572-573.
Chung H, Davis M (2012) PCR-RFLP of the ovine calpastatin gene and its association with growth. Asian J Anim Vet Adv 7: 641-652.
Collis E, Fortes MRS, Zhang Y, Tier B, Schutt K, Barendse W, Hawken R (2012) Genetic variants affecting meat and milk production traits appear to have effects on reproduction traits in cattle. Anim Genet 43:442-446.
Crow JF, Kimura M (1970)Properties of a finite population. In: An introduction to population genetics theory.The Blackburn Press, Caldwell, New Jersey: 319-365.
Duifhuis-Rivera T, Lemus-Flores C, Ayala-Valdovinos M, Sánchez-Chiprés D, Galindo-García J, Mejía-Martínez K, González-Covarrubias E (2014) Polymorphisms in beta and kappa casein are not associated with milk production in two highly technified populations of Holstein cattle in México. J Anim Plant Sci 24: 1316-1321.
Ensembl Genome Browser (2021) Caprine CAST (ENSCHIG00000027166.1) www.ensembl.org[accessed 9January 2021].
Garcia M, Michal J, Gaskins C, Reeves J, Ott T, Liu Y, Jiang Z, Maestro J, Belles X (2006) Significant association of the calpastatin gene with fertility and longevity in dairy cattle. AnimGenet 37: 304-305.
Goll DE, Thompson VF, Taylor RG, Ouali A (1998) The calpain system and skeletal muscle growth. Can J Anim Sci 78: 503-512.
Green MR, Sambrook J (2012) Isolation of high-molecular-weight DNA from mammalian cells using proteinase K and phenol. In: Molecular Cloning: A Laboratory Manual, 4, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, 47-48.
Hailu D, Mieso G, Nigatu A, Fufa D, Gamada D (2006) The effect of environmental factors on preweaning survival rate of Borana and Arsi-Bale kids. Small Ruminant Res66: 291-294.
Hanford KJ, Van Vleck LD, Snowder G (2006) Estimates of genetic parameters and genetic trend for reproduction, weight, and wool characteristics of Polypay sheep. LivestSci102: 72-82.
Hata S, Sorimachi H., Nakagawa, K., Maeda, T., Abe, K., and Suzuki, K. (2001). Domain II of m‐calpain is a Ca2+‐dependent cysteine protease. FEBS Letters 501, 111-114.
Korkmaz Ağaoğlu Ö, Çınar Kul B, Akyüz B, Elmaz Ö, Özçelik Metin M, Saatci M,ErtuğrulO (2012) Identification of β-lactoglobulin gene SacII polymorphism in Honamli, Hair and Saanen goat breeds reared in Burdur vicinity. Kafkas Univ Vet Fak Derg 18: 385-388.
Lacorte G, Machado M, Martinez M, Campos A, Maciel R, Verneque R, Teodoro R, Peixoto M, Carvalho M, Fonseca C (2006) DGAT1 K232A polymorphism in Brazilian cattle breeds. Genet Mol Res 5:475-482.
Menezes L, Sousa W, Cavalcanti-Filho E, Gama L (2016) Genetic parameters for reproduction and growth traits in Boer goats in Brazil. Small Ruminant Res136: 247-256.
Miluchová M, Gábor M, Trakovická A. (2013) Analysis of Slovak Spotted breed for bovine beta casein A1 variant as risk factor for human health. Acta Biochim Pol 60: 799-881.
Mioč B, Sušić V, Antunović Z, Prpić Z, Vnučec I, Kasap A (2011) Study on birth weight and pre-weaning growth of Croatian multicoloured goat kids. Vet Arh 81: 339-347.
Nei M, Roychoudhury A (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76: 379-390.
Ortega MS, Denicol AC, Cole JB, Null DJ, Taylor JF, Schnabel RD, Hansen PJ (2017). Association of single nucleotide polymorphisms in candidate genes previously related to genetic variation in fertility with phenotypic measurements of reproductive function in Holstein cows. J Dairy Sci 100: 3725-3734.
Palmer B, Roberts N, Hickford J, Bickerstaffe R (1998) Rapid communication: PCR-RFLP for MspI and NcoI in the ovine calpastatin gene. J Anim Sci 76: 1499-1500.
Parr T, Bardsley RG, Gilmour RS, Buttery PJ (1992) Changes in calpain and calpastatin mRNA induced by β‐adrenergic stimulation of bovine skeletal muscle. Eur J Biochem 208, 333-339.
Pringle T, Calkins CR, Koohmaraie M, Jones SJ (1993) Effects over time of feeding a β-adrenergic agonist to wether lambs on animal performance, muscle growth, endogenous muscle proteinase activities, and meat tenderness. J Anim Sci 71: 636-644.
Rashidi A, Bishop S, Matika O (2011) Genetic parameter estimates for pre-weaning performance and reproduction traits in Markhoz goats. Small Rum Res 100: 100-106.
Rupp, R., Mucha, S., Larroque, H., McEwan, J., and Conington, J. (2016). Genomic application in sheep and goat breeding. Animal Frontiers 6, 39-44.
Shelton M (1978) Reproduction and breeding of goats. J Dairy Sci 61:994-1010.
Trakovická A, Moravčíková N, Kasarda R (2013) Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochim Pol60: 783-787.
