The anxiolytic and antidepressant effect of Buxus hyrcana in the pentylenetetrazole kindled rat

Elevated Plus Maze Epilepsy Forced Swim Tests Kindling Buxus hyrcana Pentylenetetrazole
Pentylenetetrazole (PTZ) is a chemical substance which largely used for induction of seizure and epilepsy in the animal model, and it can also, disrupts free radicals balance and causes oxidative stress in the body with a negative impact on behavioral statuses like anxiety and depression. In this study, the medicinal plant Buxus hyrcana, was used to evaluate its effect on oxidative stress, anxiety and depression caused by PTZ in the rat. Twenty-four male rats were randomly allocated to 4 groups: control negative under treatment with PTZ (sub-threshold dose 35 mg/kg for one month), control positive under treatment with phenobarbital (PB-30 mg/kg), and two PTZ groups under treatment with B. hyrcana extract (BHE-300, and -600 mg/kg). For anxiety parameters, the elevated plus maze (EPM) was used. The forced swim test (FST) and rotarod test were employed to assess the antidepressant and balance potential, respectively. After behavioral evaluation, rats were anesthetized, brains were removed, and following preparation of brain homogenates, oxidative stress was evaluated using specified methods. BHE administered at the doses of 300, and 600 mg/kg, reduced immobility time in the FST exerting antidepressant-like activity. In the EPM test, BHE at the same doses, produced the anxiolytic-like effect. Also, the rats which received BHE had a significant improvement in rotarod test in contrast to control groups. In addition, brain catalase activity and superoxide dismutase level were significantly greater versus PTZ group BHE-300 treated PTZ group was significantly lower and. BHE could prevent anxiety and depression and ameliorate oxidative stress in PTZ-kindled rats.
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Aracri P, Meneghini S, Coatti A, Amadeo A, Becchetti A (2017) α4β2(*) nicotinic receptors stimulate GABA release onto fast-spiking cells in layer V of mouse prefrontal (Fr2) cortex. Neuroscience 340:48-61.
Ata A et al. (2010) Triterpenoidal alkaloids from Buxus hyrcana and their enzyme inhibitory, anti-fungal and anti-leishmanial activities. Phytochemistry 71:1780-1786.
Azizi V, Allahyari F, Hosseini A (2018) The antiepileptic and neuroprotective effect of the Buxus hyrcana Pojark hydroethanolic extract against the pentylentetrazol induced model of the seizures in the male rats.Int J Neurosci 128:913-919.
Babar ZU, Ata A, Meshkatalsadat MH (2006) New bioactive steroidal alkaloids from Buxus hyrcana. Steroids 71:1045-1051.
Bors W, Heller W, Michel C, Saran M (1990) [36] Flavonoids as antioxidants: Determination of radical-scavenging efficiencies. In: Methods in enzymology, vol 186. Elsevier, pp 343-355.
Cárdenas-Rodríguez N, Coballase-Urrutia E, Pérez-Cruz C, Montesinos-Correa H, Rivera-Espinosa L, Sampieri A, 3rd, Carmona-Aparicio L (2014) Relevance of the glutathione system in temporal lobe epilepsy: evidence in human and experimental models. Oxid Med Cell Longev 2014:759293-759293.
Choudhary KM, Mishra A, Poroikov VV, Goel RK (2013) Ameliorative effect of Curcumin on seizure severity, depression like behavior, learning and memory deficit in post-pentylenetetrazole-kindled mice. Eur J Pharmacol 704:33-40.
Choudhary MI, Shahnaz S, Parveen S, Khalid A, Majeed Ayatollahi SA, Atta Ur R, Parvez M (2003) New triterpenoid alkaloid cholinesterase inhibitors from Buxus hyrcana. J Nat Prod 66:739-742.
Choudhary MI, Shahnaz S, Parveen S, Khalid A, Mesaik MA, Ayatollahi SAM, Atta ur R (2006) New cholinesterase-inhibiting triterpenoid alkaloids from Buxus hyrcana. Chem Biodivers 3:1039-1052.
Costello DJ, Delanty N (2004) Oxidative injury in epilepsy: potential for antioxidant therapy? Expert Rev Neurother 4:541-553.
Cryan JF, Page ME, Lucki I (2005) Differential behavioral effects of the antidepressants reboxetine, fluoxetine, and moclobemide in a modified forced swim test following chronic treatment. Psychopharmacology 182:335-344.
Dhir A (2012) Pentylenetetrazol (PTZ) kindling model of epilepsy. Curr Protoc Neurosci Chapter 9:Unit9.37-Unit39.37.
Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Bahramian F, Bekhradnia AR (2010) Antioxidant and free radical scavenging activity of H. Officinalis L. var. angustifolius, V. odorata, B. hyrcana and C. speciosum. Pak J Pharm Sci 23:29-34.
Esmaeili S, Naghibi F, Mosaddegh M, Sahranavard S, Ghafari S, Abdullah NR (2009) Screening of antiplasmodial properties among some traditionally used Iranian plants. J Ethnopharmacol 121:400-404.
Ighodaro OM, Akinloye OA (2018) First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidise (GPX): Their fundamental role in the entire antioxidant defence grid Alexandria. J Med 54:287-293.
Ishige K, Schubert D, Sagara Y (2001) Flavonoids protect neuronal cells from oxidative stress by three distinct mechanisms. Free Radic Biol Med 30:433-446.
Iwamoto Y, Morinobu S, Takahashi T, Yamawaki S (2007) Single prolonged stress increases contextual freezing and the expression of glycine transporter 1 and vesicle-associated membrane protein 2 mRNA in the hippocampus of rats. Prog Neuropsychopharmacol Biol Psychiatry 31:642-651.
Khan MM, Kempuraj D, Thangavel R, Zaheer A (2013) Protection of MPTP-induced neuroinflammation and neurodegeneration by Pycnogenol. Neurochem Int 62:379-388.
Kowski AB, Weissinger F, Gaus V, Fidzinski P, Losch F, Holtkamp M (2016) Specific adverse effects of antiepileptic drugs--A true-to-life monotherapy study. Epilepsy Behav 54:150-157.
Mandhane SN, Aavula K, Rajamannar T (2007) Timed pentylenetetrazol infusion test: a comparative analysis with s.c.PTZ and MES models of anticonvulsant screening in mice. Seizure 16:636-644.
Mariani E, Polidori M, Cherubini A, Mecocci P (2005) Oxidative stress in brain aging, neurodegenerative and vascular diseases: an overview. J Chromatogr B 827:65-75.
Mesaik MA et al. (2010) Immunosuppressive activity of buxidin and E-buxenone from Buxus hyrcana. Chem Biol Drug Des 75:310-317.
Milanizadeh S, Zuwarali KNN, Aliaghaei A, Bigdeli MR (2018) Therapeutic Potential of Pretreatment with Allograft Sertoli Cells Transplantation in Brain Ischemia by Improving Oxidative Defenses. J Mol Neurosci 64:533-542.
Nassiri-Asl M, Moghbelinejad S, Abbasi E, Yonesi F, Haghighi M-R, Lotfizadeh M, Bazahang P (2013) Effects of quercetin on oxidative stress and memory retrieval in kindled rats. Epilepsy Behav 28:151-155.
Pahuja M, Mehla J, Kumar Gupta Y (2012) Anticonvulsant and antioxidative activity of hydroalcoholic extract of tuber of Orchis mascula in pentylenetetrazole and maximal electroshock induced seizures in rats. J Ethnopharmacol 142:23-27.
Pahuja M, Mehla J, Reeta KH, Tripathi M, Gupta YK (2013) Effect of Anacyclus pyrethrum on pentylenetetrazole-induced kindling, spatial memory, oxidative stress and rho-kinase II expression in mice. Neurochem Res 38:547-556.
Perry G et al. (2002) Is oxidative damage the fundamental pathogenic mechanism of Alzheimer’s and other neurodegenerative diseases? Free Radic Biol Med 33:1475-1479.
Rauca C, Zerbe R, Jantze H (1999) Formation of free hydroxyl radicals after pentylenetetrazol-induced seizure and kindling. Brain Res 847:347-351.
Saha L, Chakrabarti A (2014) Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling ina rat model.Pharmacol Biochem Behav 120:57-64.
Stadtman ER (2001) Protein oxidation in aging and age‐related diseases. Ann N Y Acad Sci 928:22-38.
Sudha K, Rao AV, Rao A (2001) Oxidative stress and antioxidants in epilepsy. Clin Chim Acta 303:19-24.
Waldbaum S, Patel M (2010) Mitochondria, oxidative stress, and temporal lobe epilepsy. Epilepsy Res 88:23-45.
Wollenweber E, Rustaiyan A (1991) Exudate flavonoids in three Persian asteraceae species.Biochem Syst Ecol 19:673-675.
Zhao R-r, Xu X-c, Xu F, Zhang W-l, Zhang W-l, Liu L-m, Wang W-p (2014) Metformin protects against seizures, learning and memory impairments and oxidative damage induced by pentylenetetrazole-induced kindling in mice.Biochem Biophys Res Commun 448:414-417.