Antibiotic resistant shiga toxin producing Escherichia coli isolates from milk and milk products

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Published: Apr 18, 2023
Updated: 2023-04-18
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2023-04-18 (2)
DOI: https://doi.org/10.12681/jhvms.29111
Keywords:
Shiga toxin producing E. coli (STEC) antibiotic resistant (AR) genes (blaTEM and blaCMY2)
AMK Sobeih
AA Moawad
T Sharshar
Agricultural Research Center
Abstract

Totally  450 milk and milk products (50 each of raw buffalo & cow milk; Kareish, Tallaga and Roquefort cheeses; yoghurt; rayeb; condensed milk and sour cream) samples were randomly purchased from various markets in Kafr El-Sheikh for isolation and identification of STEC. Results showed that 60%, 80%, 30%, 10%, 12%, 20%, 6% and 22% of buffalo & cow milk, Kareish, Roquefort, Tallaga, yoghurt, rayeb and sour cream respectively, contained E.coli, but negative in condensed milk. Fifty suspected isolates were serologically identified using E.coli O157:H7 kits, and confirmed in 69.2%, 53.8%, 60%, 16.7% and 20% isolates of buffalo & cow milk, Kareish, Tallaga and yoghurt, while was negative in Roqufort, rayeb, and sour cream. Using PCR assay, 21 confirmed isolates were examined, 44.4, 71.4, 66.7, 100 and 100% isolates of buffalo & cow milk, Kareish, Tallaga and yoghurt were positive for rfbE gene; while 55.6, 42.8, 66.7, 100 and 100 % isolates of buffalo & cow milk, Kareish, Tallaga and yoghurt were positive for blaTEM, but all isolates were negative for blaCMY2. Consequently, raw milk and most dairy products including fermented products were possible source of E.coli O157:H7food poisoning, so usage of accurate hygienic measures through manufacture and retail of milk products be essential.

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References
Ahmed AM and Shimamoto T (2015) Molecular analysis of multidrug resistance in shiga toxin producing Escherichia coli O157:H7 isolated from meat and dairy products. Int J Food Microbiol 16 (193) 68-73.
Ali, T.; Rahman, S.; Zhang, L.; Shahid, M.; Zhang, S.; Liu, G.; Gao, J.; and Han, B. (2016). ESBL Producing Escherichia coli form cows suffering mastitis in China contain clinical class 1 Intergons with CTX_M Linked to ISCR1. J. Front Microbiol.
Amézquita-López, B.A.; Quiñones, B.; Soto-Beltrán, M.; Lee, B.G.; Yambao, J.C.; Lugo-Melchor, O.Y. and Chaidez,C.; (2016). Antimicrobial resistance profiles of shiga toxin producing Escherichia coli O157 and non O157 recovered from domestic farm animals in rural communities in Northwestern Mexico. J. Antimicrob. Resist Infect Control.
APHA (American Public Health Association) (2004). Standard methods for the examination of dairy products, 17th ed. American public health Association. Washington DC, USA.
Cardozo, M.V.; Nespolo, N.; Delfino, T.C.; de Almeida, C.C.; pizauro, L. J. L.; Valmorbida, M.K.; Pereira, N. and de Avila, F.A. (2021). Raw milk cheese as a potential infection source of pathogenic and toxogenic food born pathogens. Food sci. Technol. Campinas, 41(2): 355-358.
Chapman, P.A.; Wright, D.J. and Siddons C.A. (1994). A comparison of immune magnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from bovine faeces. J. Med. Microbiol.40(6):424-427
Clinical Laboratory Standards Institute (CLSI) (2006). Performance standards for antimicrobial Disk susceptibility tests, Approved Standard – 9th Ed. (M2-Ag), Clinical and laboratory standards Institute, Wayne, Pa, USA.
Colom, K.; Pèrez, J.; Alonso, R.; Fernández-Aranguiz, A.; Lariňo, E.; and Cisterna, R. (2003). Simple and reliable multiplex PCR assay for detection of blaTEM,blaSHV and blaOXA-1 genes in Enterobacteriaceae. FEMS Microbiol. Letters J. 223(2):147-151.
Cortés, M.P.; Blanc, V.; Mora, A.; Dahbi, G.; Blanco, J. E.; Blanco, M.; Lopez, C.; Andreu, A.; Navarro, F.; Alonso, P.M.; Bou,G.; Blanco, J. and Liagstera, M. (2010). Isolation and characterization of potentially pathogenic antimicrobial-resistant Escherichia coli strains from chicken and pig farms in Spain. Appl. and Environ. Microbiol. J. 76(9):2799–2805.
De Boer, E. and Heuvelink, A.E. (2000). Methods for the detection and isolation of shiga toxin – producing Escherichia coli. Soc. Appl. Microbiol. J. 88(29): 133-143.
El Gamal, A.M.; Abdou, M.S.; E.bied, N.A. and Salem, N.I (2019). Isolation and molecular identification of E.coli O157 from raw milk and some milk products in KafrElsheikh governorate. Anim. Health Res.J. 3(1): 283-289
Elbastawesy, A.M. ; . Deeb, A.M.M. and El-desoky, N.I. (2016). Incidence of E.coli O157:H7 in some dairy products. Kafrelsheikh Vet. Med. 19(14): 315-328.
El nahas, A.W.; Mohamed, H.A.; Elbarbary, H.A. and Mohamed, H.S. (2015). Incidence of Ecoli in raw milk and its products. Benha Vet. Med. J. 29(1):112-11
FDA (2009). Summary report on antimicrobials sold or distributed for use in food producing animals. Retrived from http://www.fda.gov/downloads/farindustry/userfees/animaldruguserfeeactadufa/UCm231851.pdf
Food and Agriculture organization of the United Nations World Health Organization (FAO/WHO) (2018). Microbiological risk assessment, shiga toxin producing Escherichia coli (STEC) and food attribution, characterization and monitoring, chapter 4 – hazard identification and characterization, series 31.
Forsberg, K.J.; Reyes, A.; Wang, B.; selleck, E.M.; Sommer, M.O., and Dantas, G. (2012). the shared antibiotic resistance of soil bacteria and human pathogens. Science J. 337(6098): 1107-1111.
Foster, G.; Hopkins, G.F.; Gunn, G.J.; Ternet, H.E.; Thoson-Carter, F.; Knight, H.T.; Garaham, D.J.; Edge, V. and Synge, B.A. (2003). Comparison of two pre-enrichment media prior to immunomagnetic separation for isolation of Escherichia coli O157 from bovine feces. J. Apll. Microbiol. 95:2513-2516.
Franz, E.; Klerks, M.M. ; Devos, O.J. ; Termorshuizen, A. J. and van- Bruggen, A.H.C. (2007). Prevalence of shiga toxin – producing Escherichia coli stx1, stx2, eaeA, and rfbE genes and survival of E.coli O157:H7 in Manure from organic and low- Input convention in Dairy farms. APPl. And environ. Microbiol. J. 73(7): 2180-2190.
Garbaj, A.M.; Awad, E.M.; Azwai, S.M.; Abolghait, S.K.; Naas, H.T.; Moawad, A.A.; Gammoudi, F.T.; Barbieri, I. and El-daghayes, I.M. (2016). Enterohemorrhagic Escherichia coli O157 in milk and dairy products from Libya: Isolation and molecular identification by partial sequence of 16S rDNA. Vet. World J. 9(11):1184-1189.
Garcia, P.G.; Silva, V.L. and Diniz, C.G. (2011). Occurrence and antimicrobial drug susceptibility patterns of commensal and diarrhea genic Escherichia coli in fecal micro biota from children with and without acute diarrhea. The J. of Microbiol. 49(1):46-52.
Hassan, G.M.; Meshref , A.M.S.; El-Newery, H.A. and Mohamed, A.S (2021). Prevalence of Escherichia coli in milk and some Dairy products in Beni-Suef Governorate, Egypt. J. vet. Med. Res. 27(2):161-167.
Hassan, M.N.A.; Mehriz, A.M.; Salem, A.S. and Abozeid, H.H. (2017). Formulation and characterization aspects of light sour cream. J. Food and Dairy sci. Mansoura Univ. 8(6): 257-262.
Heijnen, L. and Medema, G. (2006). Quantitative detection of E. coli, E. coli O157 and other shiga toxin producing E. coli in water samples using a culture method combined with real-time PCR. Jornal of Water Health. (4):487-498.
Ibrahim, E.M.A. and Sobeih, A.M. (2006). Diarrhogenic E.coli in Kareish cheese manufactured by different methods with special reference to E.oli O157:H7. Minfya Vet. Med. J. 4, 169-178.
Jiang, J. ; Larkin, C.; steele, M.; Poppe, C. and Odumeru. J.A. (1998). Evaluation of universal pre enrichment broth for the recovery of foodborne pathogens from milk and cheese. J. dairy sci. 81(11):2798 – 2803.
Kok, T.; Worswich, D. and Gowans, E. (1996). Some serological techniques for microbial and viral infections. In Practical Medical Microbiology (Collee, J.; Fraser, A.; Marmion, B. and Simmons, A., eds.), 14th ed., Edinburgh, Churchill Livingstone, UK.
Law,D. (2000). virulence factors of Escherichia coli O157 and other shiga toxin producing E.coli. J. of APPL. Micro. 88(5):729-745
Leininger, D.I,; Roberson, I.R. and Elvinger, F. (2001). Use of eosin methylene blue agar to differentiate Escherichia coli from other gram negative mastitis pathogens. J. Vet. Diagn. Invest. 13: 273-275.
Losada, L.; DebRoy, C.; Radune, D.; Kim, M.; Sanka, R.; Brinka C,L; kanyawasam, S.; Shelton, D. ; Fratamico, P.M. ; Kapur, V. and Feng, P.C.H. (2016). Whole genome sequencing of diverse shiga toxin producing and nonproducing Escherichia coli strains reveals a variety of virulence and novel antibiotic resistance plasmids. Microorganisms J. 8(3): 8-11.
Mahanti, A.; Samanta, I.; Bandopaddhay, S.; Joardar, S.M.; Dutta, T.K.; Batahyal, S.; Sar, T.K.; Isore, D.P. (2013). Isolation, molecular characterization and antibiotic resistance of shiga toxin producing Escherichia coli (STEC) from buffalo in India. Letters in APPL. Mirobiol. J. 56(4):291-298.
March, S.B. and Ratnam, S. (1986). Sorbitol – MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J. Clinic. Microbiol. 23(5):869-872.
Mir, R.A. and Kudva, I.T. (2019). Antibiotic resistant shiga toxin producing Escherichia coli: An over view of prevalence and intervention strategies. Zoonoses and Public Health J. 66(1):1-13.
Munita, J.M. and Arias, C.A. (2016). Mechanisms of Antibiotic resistance. Microbiol. Spectr. J. 4(2): 10.1128.
Pennington, H. (2010). Escherichia coli O157. Lancet. J. 376(9750):1428-1435.
Pérez-Pérez, F.J. and Hanson, N.D. (2002). Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J. of Clinc. Microbiol. 40(6): 2153–2162.
Prencipe, V.; Migliorati, G.; Matteuci, O.; Colistri, P. and Di Giannatale, E. (2010). Assessment of hygienic quality of some types of cheese sampled from retail outlets. Vet. Ital. 46(2):233-242.
Richard, W.; Trepta and Stephen, C.E. (1984). Methylumbelliferyl-D-Glucuronide-Based Medium for Rapid Isolation and Identification of Escherichia coli. J. of Clinic. Microbiol. 19(2):172-174.
Salguero, J.F.; AlCala, M.; Marcos, A. and Esteban, M. (1986). Measurement and calculation of water activity in blue cheese. J. Dairy Res. 52:639-644.
Sambrook, J.; Fritscgh, E.F. and Mentiates (1989). Molecular coloning. A laboratory manual. Cold spring Harbor Laboratory press, New York.