Tuberculin test errors and its effect on detection of bovine tuberculosis

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DOI: https://doi.org/10.12681/jhvms.29357
Keywords:
Bovine Post-mortem Tuberculin Tuberculosis Egypt
M BORHAM
Assistant Researcher, Bacteriology Department, Animal Health Research Institute Matrouh Lab, Egypt
A OREIBY
Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
A EL-GEDAWY
Chief Researcher, Bacteriology Department, Animal Health Research Institute, Egypt
Y HEGAZY
Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
M AL-GAABARY
Abstract

Bovine tuberculosis is an endemic disease in Egypt, a notable gap exists between limited cases identified by single intradermal tuberculin test performed through the national control program and higher detected cases at abattoirs. Therefore, this study aimed to investigate epidemiological situation and causes of the previously mentioned gap in Middle-Delta Region. A total of 25 emergency-slaughtered animals of unknown tuberculosis-status were investigated by cocktail-antigens ELISA and post-mortem examination. Five visible tuberculous-lesion cases were detected and confirmed by PCR, ELISA was sensitive and predictive of the existence of tuberculous-lesions; 4(80%) out of 5 visible-lesion cases were seropositive. True prevalence among the slaughtered animals was 27%. In addition, tuberculin-testing of 400 animals during the national control program was evaluated, many technical and procedural errors were detected, and all animals were negative.Out of them, 55 animals were tested by ELISA before the application of tuberculin test, 30 (54.5%) animals were seropositive. To confirm the effect of the reported errors on reliability of tuberculin test, reference serum of 20 tuberculosis-positive animals that were tested by standard-procedures tuberculin test and their status were confirmed by PCR after slaughtering, were tested by ELISA. A complete matching was evident, the 20 standard-tuberculin positive animals were all seropositive. In conclusion, bovine tuberculosis is endemic at high levels in the study area, reported errors of tuberculin test during national control program may be the cause for missing tuberculosis cases and not tuberculin test itself and finally, a further wide-scale funded study is required to discover the situation throughout Egypt.

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References
Abdellrazeq GS, Elnaggar MM, Osman HS, Davis WC, Singh M (2016) Prevalence of Bovine Tuberculosis in Egyptian Cattle and the Standardization of the Interferon‐gamma Assay as an Ancillary Test. TransboundEmerg Dis 63(5): 497-507.
Abou-Eisha AM, El-Attar AA, El-Sheary MN (2002) Bovine and atypical mycobacterial infections of cattle and buffaloes in Port Said Province, Egypt. Assiut Vet Med J 47: 152-162.
Amin A (2019)Bovine Tuberculosis in Egypt. In: Tuberculosis in Animals: An African Perspective, Springer Nature Switzerland AG, pp. 305-315.
Bernitz N, Clarke C, Roos EO, Goosen WJ, Cooper D, van Helden PD, Parsons SD, Miller MA (2018) Detection of Mycobacterium bovis infection in African buffaloes (Synceruscaffer) using QuantiFERON ®-TB Gold (QFT) tubes and the Qiagen cattletype® IFN-gamma ELISA. Vet Immunol Immunopathol 196: 48-52.
Bezos J, Casal C, Romero B, Schroeder B, Hardegger R, Raeber AJ, López L, Rueda P, Domínguez L (2014) Current ante-mortem techniques for diagnosis of bovine tuberculosis. Res Vet Sci 97: S44-S52.
Borham, M., Oreiby, A., El‐Gedawy, A., Hegazy, Y., Hemedan, A., & Al‐ Gaabary, M. (2021). Abattoir survey of bovine tuberculosis in tanta, centre of the Nile delta, with in silico analysis of gene mutations and protein-protein interactions of the involved mycobacteria. Transboundary and Emerging Diseases. DOI: 10.1111/tbed.14001
Bovine Tuberculosis Manual (2016) Agriculture, Forestry and Fisheries Department. Republic of South Africa.
Brito BP, Aly SS, Anderson RJ, Fossler CP, Garry FB, Gardner IA (2014) Association between caudal fold tuberculin test responses and results of an ELISA for Mycobacterium avium subsp paratuberculosis and mycobacterial culture of feces in tuberculosis-free dairy herds. J Am Vet Med Assoc 244(5): 582-587.
Buyuk F, Bozukluhan K, Saglam AG, Gokce G, Celebi O, Celik E, Kiziltepe S, Coskun MR, Otlu S, Sahin M (2017) The prevalence estimates of Mycobacterium bovis infection in cattle with ELISA. J Hell Vet Med Soc 68(4): 541-546.
Casal C, Infantes JA, Risalde MA, Díez-Guerrier A, Domínguez M, Moreno I, Romero B, De Juan L, Sáez JL, Juste R, Gortázar C (2017) Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle. Res Vet Sci 112: 214-221.
Domingo M, Vidal E, Marco A (2014) Pathology of bovine tuberculosis. Res Vet Sci 97: S20-S29.
Ellner JJ(1996)Immunosuppression in tuberculosis. Infect Agents Dis 5:62.
El-Mahrouk AM, El-Balawy MB (2010) Bacteriological, Serological, Molecular characterization and risk assessment of bovine tuberculosis in cattle. Egypt J Comp Path& Clinic Path 23(1): 64-84.
Fontana S, Pacciarini M, Boifava M, Pellesi R, CastoB ,Gastaldelli M, Koehler H, Pozzato N,Casalinuovo F, Boniotti MB (2018) Development and evaluation of two multi-antigen serological assays for the diagnosis of bovine tuberculosis in cattle. J Microbiol Methods 153:118-126.
Global Tuberculosis Report (2018)WHO, PP.1-3.
Hassanain NA, Hassanain MA, Soliman YA, Ghazy AA, Ghazyi YA (2009) Bovine tuberculosis in a dairy cattle farm as a threat to public health. Afr J Microbiol Res 3(8): 446-450.
Humblet MF, Walravens K, Salandre O, Boschiroli ML, Gilbert M, Berkvens D, Fauville-Dufaux M, Godfroid J, Dufey J, Raskin A, Vanholme L (2011) Monitoring of the intra-dermal tuberculosis skin test performed by Belgian field practitioners. Res Vet Sci 91(2): 199-207.
Infantes-Lorenzo JA, Moreno I, Roy A, Risalde MA, Balseiro A, de Juan L, Romero B, Bezos J, Puentes E, Åkerstedt J, Tessema GT (2019) Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations. BMC vet Res 15(1): 70.
Infantes-Lorenzo JA, Moreno I, de los ÁngelesRisalde M, Roy A, Villar M, Romero B, Ibarrola N, de la Fuente J, Puentes E, de Juan L, Gortázar C (2017) Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis. Clin Proteome 14(1): 36.
Khoudair RM, Ibrahim EM, Saker GG, Hafez MA (2009)Clinicodiagnostic and pathological studies on cattle and buffaloes suffering from brucellosis and tuberculosis in KafrEl Sheikh Governorate. Egypt. J Comp Path Clinic Path 22(1): 148-174.
Koni A,Juma A, Morini M, Nardelli S, Connor R, Koleci X (2016) Assessment of an ELISA method to support surveillance of bovine tuberculosis in Albania. Ir Vet J 69(1): 11.
McCallan L,Brooks C,Couzens C, Young F, Byrne AW, McNair J (2017) Performance of serological antibody tests for bovine tuberculosis in cattle from infected herds in Northern Ireland. BioRxiv, 235184.
Nuñez-Garcia J, Downs SH, Parry JE, Abernethy DA, Broughan JM, Cameron AR, Cook AJ, de la Rua-Domenech R, Goodchild AV, Gunn J, More SJ, Rhodes S, Rolfe S, Sharp M, Upton PA, Vordermeier HM, Watson E, Welsh M, Whelan AO, Woolliams JA, Clifton-Hadley RS, Greine M (2018) Meta-analyses of the sensitivity and specificity of ante-mortem and post-mortem diagnostic tests for bovine tuberculosis in the UK and Ireland. Prev Vet Med 1(153): 94-107.
Oreiby AF, Hegazy, YM (2016) Diagnosis of ovine caseous lymphadenitis by blood and milk gamma interferon assays. Small Rumin Res 144:109-112.
Oreiby AF (2015) Diagnosis of caseous lymphadenitis in sheep and goat. Small Rumin Res 123: 160-166.
Picasso-Risso C, Gil A, Nunez A, Suanes A, Macchi V, Salaberry X, AlvarezJ, Perez A (2019) Diagnostic interaction between bovine tuberculosis (bTB) and Johne’s disease in bTB highly prevalent dairy farms of Uruguay. Vet Anim Sci 7: 100052.
Riad EM(2015) Overview on the Diagnostic Tools of Bovine Tuberculosis in Egypt. EC Microbiol 2: 243-246.
Sahli H, Mouelhi A, Diouani MF, Tlig L, Refai A, Landoulsi RB, Sayadi M, Essafi M (2018) An advanced intelligent ELISA test for bovine tuberculosis diagnosis. Biomed Signal Proces 46: 59-66.
Souza II,Melo ES, Ramos CA, Farias TA, Osório AL, Jorge KS, Vidal CE, Silva AS, Silva MR, Pellegrin AO, AraújoFR(2012) Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis. Springerplus 1: 1-6.
Souza IIF,de Arruda Rodrigues R, Jorge KSG, Silva MR,Lilenbaum W, Vidal CES,Etges RN, Kostovic M, Araujo FR (2019) ELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle. J Vet Med Sci 81(1):9-14.
Trost B,Stuber T, Surujballi O, Nelson J, Robbe-Austerman S, Smith NH, Desautels L, Tikoo SK, Griebel P (2016) Investigation of the cause of geographic disparities in IDEXX ELISA sensitivity in serum samples from Mycobacterium bovis-infected cattle. Sci Rep 6: 22763.
Waters WR,Vordermeier HM, Rhodes S, Khatri B, Palmer MV, Maggioli MF, Thacker TC, Nelson JT, Thomsen BV, Robbe-Austerman S, Garcia DMB (2017) Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results. BMC Vet Res 13(1): 164.
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