A report on less-severe, long-duration persistent hind-limb ischemia surgical rabbit model

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DOI: https://doi.org/10.12681/jhvms.29395
Keywords:
Hind Limb Ischemia Rabbit Model Welfare Thigh muscle atrophy Angiography
VS HARIKRISHNAN
Division of Laboratory Animal Science, Department of Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
SJ SHENOY
Division of in vivo models and testing,Department of Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India
VR RANARAJ
Veterinary Surgeon, Department of Animal Husbandry, Government of Kerala
S SUKHIJA
Department of Molecular and Cellular Medicine Institute of Liver and Biliary Sciences (ILBS) Vasant Kunj NewDelhi
AK OLOYO
Department of Physiology, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
Abstract

Development of a persistent hind limb ischemia rabbit model without excessive adverse symptoms and compromised animal welfare. New Zealand White (n=18, male and female) rabbits of 3-4 months of age and 3.0± 0.1 Kg body weight were used. The surgical technique for ischemia avoided the ligation of vessels above the inguinal ligament and included only the ligation and complete excision of the common and superficial femoral arteries along with all their branches up to popliteal and saphenous arteries. Study duration was 84 days. All animals completed the study period uneventfully. The activity of the animals remained unaffected throughout the study except for the first post-operative day. Adverse symptoms of other models such as loss of limb due to necrosis, loss of nails and necrosis of skin were not observed while successful ischemia was confirmed. There was a significant decrease (P=0.0381) in ischemic right limb circumference.Terminal angiography by abdominal aortic cannulation in the animals demonstrated negligible amount of angiogenesis at the distal ischemic thigh in comparison to the control limb (P=0.001). This study reports successful development of a refined chronic hindlimb ischemia rabbit model.

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