Investigation of bovine interleukin-6 gene polymorphism and its association with Cryptosporidium infection in calves

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DOI: https://doi.org/10.12681/jhvms.29429
Keywords:
SNP interleukin-6 phylogenetic tree calves
M MESHKAT
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
B SHEMSHADI
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
K AMINI
Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
Abstract

Interleukin-6 (IL-6) is associated with inflammatory diseases, but its connection with Cryptosporidium in Holstein calves remains unknown. This study aimed to investigate the effect of single nucleotide polymorphisms (SNPs) of IL-6 on the resistance and susceptibility to Cryptosporidium in calves and to prepare a phylogenetictree in order to show the relation between Cryptosporidium species. Seventy-two samples were studied from healthy and infected with Cryptosporidium calves and genotyped using the tetra amplification refractory mutation system (ARMS). The phylogenetic tree was constructed by the neighbor-joining method using the MEGA 7.0 software. The results showed a frequency of 76.40% for T allele and 23.60% for C allele in the healthy calves, while the results showed a frequency of 73.60% for T allele and 26.40% for C allele in calves infected with Cryptosporidium. The results did not reveal a significant difference between healthy and infectious animals according to the allele frequency (P=0.637). The phylogenetic tree demonstrated that C. parvum (HQ259589.1) with an 81% bootstrap were clustered with C. hominis (KM012041.1). The results also indicated that C. parvum (HQ259589.1) and C. hominis (KM012041.1) had a common ancestor with C. cuniculus. Additionally, C. andersoni(HQ259590.1) with an 88% bootstrap of support was placed in the same clade of C. muris (L19069.1), and both of them had a common ancestor with C. serpentis(KF240618.1). Further studies are required to investigate the relation between SNPs of IL-6 in other regions and the resistance or susceptibility to Cryptosporidium in calves.

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References
Alsmark C, Nolskog P, Angervall AL, Toepfer M, Winiecka-Krusnell J, Bouwmeester J (2018) Two outbreaks of cryptosporidiosis associated with cattle spring pasture events. Vet ParasitolReg Stud Rep 14:71-74.
Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, SaifLJ (2006)Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. J Virol 80: 372-382.
Blake RT (2009) Identifying Polymorphisms in Bovine Interleukin-6. In partial fulfillment of therequirements for theCASNR Honors Program.
Cai J, Collins MD, McDonald V, Thompson DE (1992) PCRcloning and nucleotide sequence determination of the 18SrRNA genes andinternal transcribed spacer 1 of the protozoan parasites Cryptosporidiumparvum and Cryptosporidium muris. BiochimBiophysActa 1131:317-320.
Deng N, Zhou H, Fan H, Yuan Y (2017). Single nucleotide polymorphisms and cancer susceptibility.Oncotarget 8(66):110635-110649.
de Silva DG, Mendis LN, Sheron N, Alexander GJ, Candy DC, Chart H, Rowe B (1993) Concentrations of interleukin 6 and tumour necrosis factor in serum and stools of children with Shigelladysenteriae 1 infection. Gut 34: 194-198.
Diez-Fraile A, Meyer E, Burvenich C (2003) Sympathoadrenal and immune system activation during the periparturient period and their association with bovine coliform mastitis. TheVet Quarter 25: 31-44.
Dienz O, Rincon M (2008) The effects of IL-6 on CD4 T cell responses. ClinImmunol 130: 27-33.
Donath MY, Shoelson SE (2011) Type 2 diabetes as an inflammatory disease. Nat Rev Immunol 11:98-107.
Firoozi Z, Sazmand A, Zahedi A, Astani A, Fattahi‑Bafghi A, Kiani‑Salmi N, Ebrahimi B, Dehghani‑Tafti A, Ryan U, Akrami‑Mohajeri F (2019) Prevalence and genotyping identification of Cryptosporidium in adult ruminants in central Iran. Parasites Vectors 12:510. https://doi.org/10.1186/s13071-019-3759-2.
Ghavimi R, Sharifi M, Mohaghegh MA, Mohammadian H, Khadempar S, Rezaei H (2016) Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population. Adv Biomed Res 5:18.
Jiang B, Snipes-Magaldi L, Dennehy P, Keyserling H, Holman RC, Bresee J, Gentsch J, GlassRI (2003)Cytokines as mediators for or effectors against rotavirus disease in children. ClinDiagn Lab Immunol 10: 995-1001.
Karpman D, Andreasson A, Thysell H, Kaplan BS, SvanborgC (1995) Cytokines in childhood hemolytic uremic syndrome and thrombotic thrombocytopenic purpura Pediatr. Nephrol 9: 694-699.
Kumar A, Ghosh B (2008) Genes and Immunity. A single nucleotide polymorphism (A -> G) in intron 3 of IFN gamma gene is associated with asthma. Gene Immun 9:294-301.
Lacroix S, Mancassola R, Naciri M, Laurent F (2001) Cryptosporidium parvum-specific mucosal immune response in C57BL/6 neonatal and gamma interferon-deficient mice: role of tumor necrosis factor alpha in protection. Infection Immun 5: 1635-1642. https://doi.org/10.1128/IAI.69.3.1635-1642.2001.
Naushad S, Adeolu M, Goel N, Khadka B, Al-Dahwi A, Gupta RS (2015a) Phylogenomic and molecular demarcation of the core members of the polyphyletic Pasteurellaceae genera Actinobacillus, Haemophilus, and Pasteurella. Int J Genom43: 198560. https://doi.org/10.1155/2015/198560.
Naushad S, Adeolu M, Wong S, Sohail M, Schellhorn HE, Gupta RS (2015b) A phylogenomic and molecular marker based taxonomic frame work for the order Xanthomonadales: proposal to transfer the families Algiphilaceae and Solimonadaceaeto the order Nevskialesord. nov.and to create a new family with in the order Xanthomonadales, the family Rhodanobacteraceae fam. nov., containing the genus Rhodanobacterand its closest relatives. Antonie Van Leeuwenhoek107:467-485. https://doi.org/10.1007/s10482-014-0344-8.
Rincon M (2012) Interleukin-6: From an inflammatory marker to atarget for inflammatory diseases. Trend Immunol 33:571-577.
Saki J, Asadpouri R (2017) Molecular characterization of Cryptosporidium species isolated from cattle in southwest of Iran. Jundishapur J Microbiol 11(5):e59371. https://doi.org/10.5812/jjm.59371.
Sanghera DK, Manzi S, Bontempo F, Nestlerode C, Kamboh MY (2004) Role of an intronic polymorphism in the PDCD1 gene with the risk of sporadic systemic lupus erythematosus and the occurrence of antiphospholipid antibodies. Human Gen 115: 393-398.
Stangl K, Cascorb I, Laule M, Klein T, Stangl V, Rost S, WerneckeKD, Felix SB, Bindereif A, Baumann G, Roots I (2000) High CA repeat numbers in intron 13 of the endothelial nitric oxide synthase gene and increased risk of coronary artery disease. Pharmogenetic 10: 133-140.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA 6: Molecular Evolutionary Genetics Analysis version 6.0. MolBiolEvol 30: 2725-2729. https://doi.org/10.1093/molbev/mst197.
Thomson S, Hamilton CA, Hope JC, Katzer F, Mabbott NA, Morrison LJ, Innes EA (2017) Bovine cryptosporidiosis: impact, host‑parasite interaction and control strategies.Vet Res 48:42-58. https://doi.org/10.1186/s13567-017-0447-0.
Tzipori S, Griffiths JK (1998) Natural history and biology of Cryptosporidium parvum. AdvParasitol 40:5-36.
Widmer G, Lee Y, Hunt P, Martinelli A, Tolkoff M, Bodi K (2012) Comparative genome analysis of two Cryptosporidium parvum isolates with different host range. Infect Genet Evol 12(6): 1213-1221. https://doi.org/10.1016/j.meegid.2012.03.027.
Yang Z, Fu Y, Gong P, Zheng J, Liu L, Yu Y, Li J, Li H, Yang J, Zhang X (2015) Bovine TLR2 and TLR4 mediate Cryptosporidium parvum recognition in bovine intestinal epithelial cells. MicrobPathog 85:29-34.
Ye S, Dhillon S, Ke X, Collins AR, Day INM (2001) An efficient procedure forgenotyping single nucleotide polymorphisms. Nucleic Acids Res 29:e88.
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