Effects of the addition of flower honey and pine honey to extenders on spermatological characteristics in ram semen

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Published: Jan 11, 2024
DOI: https://doi.org/10.12681/jhvms.29579
Keywords:
ram semen semen cryopreservation extender flower honey pine honey
H Yüksel
Graduate Education Institute, Istanbul University-Cerrahpasa, Avcılar, Istanbul, Turkey
https://orcid.org/0000-0003-4490-1372
A Eser
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey
https://orcid.org/0000-0003-1326-2678
R Arıcı
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul UniversityCerrahpasa, Avcılar, Istanbul, Turkey
https://orcid.org/0000-0002-2236-2526
S Yağcıoğlu
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul UniversityCerrahpasa, Avcılar, Istanbul, Turkey
https://orcid.org/0000-0002-9355-498X
K Ak
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul UniversityCerrahpasa, Avcılar, Istanbul, Turkey
https://orcid.org/0000-0002-4053-9655
Abstract

Bu çalışmada, belirli oranlarda çiçek ve çam balının koç spermatının spermatolojik parametreleri üzerine etkileri araştırılmıştır. Tris-yumurta sarısı bazlı meni sulandırıcıya her bal türü için %1.0 (h/h) ve %2.5 (h/h) oranlarında çiçek ve çam balı eklenmiştir. Sperm toplama için beş Kıvircik koç kullanıldı. Toplanan spermler havuzlamanın ardından 5 eşit parçaya bölündü. %1.0 (h/h) ve %2.5 (h/v) çam balı ve %1.0 (h/v) ve %2.5 (h/h) çiçek balı oranlarında ve bal ilavesi yapılmadan iki aşamalı seyreltme yöntemi kullanılmıştır. Tris-yumurta sarısı bazlı meni genişleticiye. Numuneler daha sonra sıvı nitrojen buharında dondu ve sıvı nitrojen içinde saklandı. Semen canlılığı parametreleri (toplam ve progresif motilite, hız, kinetik hareket parametreleri ve mitokondriyal aktivite) ve morfolojik bütünlük parametreleri (Hancock fiksasyon testi, akış sitometrisi ve floresan boyama) dengeleme ve çözdürmeden sonra değerlendirildi. Çalışma boyunca on tekrarlı çalışma yapılmıştır.

Sonuçlar çiçek balının çözülme sonrası canlılık parametreleri üzerinde özellikle %2,5 (h/h) oranında faydalı etkiye sahip olduğunu göstermiştir. Her oranda ve her çeşit bal içeren sulandırıcılar, çözüldükten sonra zar bütünlüğünü korumuştur. Tris-yumurta sarısı bazlı sulandırıcıya %1.0 (v/v) çiçek balı ilavesi, diğer gruplara kıyasla Hancock fiksasyon testi ile akrozomal bütünlük açısından daha iyi sonuçlar vermiştir (p < 0.05). Sonuç olarak, Tris-yumurta sarısı sulandırıcısına %1.0 (h/h) ve %2.5 (h/v) çiçek balı ilavesiyle çözülme sonrası spermatolojik parametrelerde daha iyi sonuçlar kaydedildi.

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References
Abdullah MH, Selman MO, Fakhrildin MBM (2015) Effect of honeybee and coq10 supplemented to cryopreservation solution on freeze-thawed human sperm parameters. World J Pharm Res 4(12): 524-534.
Aisen EG, Alvarez HL, Venturino A, Garde JJ (2000) Effect of trehalose and EDTA on cryoprotective action of ram semen diluents. Theriogenology 53: 1053-1061.
Aisen EG, Medina VH, Venturino A (2002) Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology 57(7); 1801-1808.
Ak K, Cirit Ü, Nur Z, Bacınoğlu S, Pabuccuoğlu S, Özdaş Ö et al. (2010) Effects of extender osmolarity, cooling rate, dilution rate and glycerol addition time on post-thaw ram semen characteristics and fertilization. J Fac Vet Med Istanbul Univ 36(2): 33-46.
Akbulut M, Özcan MM, Çoklar H (2009) Evaluation of antioxidant activity, phenolic, mineral contents and some physicochemical properties of several pine honeys collected from Western Anatolia. Int J Food Sci Nutr 60(7): 577-589.
Amann RP, Waberski D (2014) Computer-assisted sperm analysis (CASA): capabilities and potential developments. Theriogenology 81(1): 5-17.
Amann RP (1989) Can the fertility potential of a seminal sample be predicted accurately?. J Androl 10(2): 89-98.
Bacinoğlu S, Cirit Ü, Nur Z, Ak K (2007) Eritilmiş koç spermasında farklı gliserol katma tekniklerinin ve soğutma hızının spermatolojik özelliklere etkisi. J Fac Vet Med Istanbul Univ 33(1): 11-21.
Bacinoglu S, Taş M, Cirit Ü, Özdaş, ÖB, Ak K (2008) The potential fertility estimation capacity of the hypoosmotic swelling test, the thermal stress test and a modified cervical mucus penetration test in the bovine. Anim Reprod Sci 104(1): 38-46.
Bogdanov S, Jurendic T, Sieber R, Gallmann P (2008) Honey for nutrition and health: a review. J Am Coll Nutr 27(6): 677-689.
Cámara DR, Silva SV, Almeida, FC, Nunes, JF, Guerra, MMP (2011) Effects of antioxidants and duration of pre-freezing equilibration on frozen-thawed ram semen. Theriogenology 76(2): 342-350.
Cirit Ü, Bağış H, Demir K, Agca C, Pabuccuoğlu S, Varışlı Ö et al. (2013) Comparison of cryoprotective effects of iodixanol, trehalose and cysteamine on ram semen. Anim Reprod Sci 139: 38–44.
Demir K, Öztürk Bakırer G, Cirit Ü, Bozkurt HH, Aktaş A, Birler S et al. (2015) Effects of cooling rate on membrane integrity and motility parameters of cryopreserved ram spermatozoa. Kafkas Univ Vet Fak Derg 21: 61–67.
El-Nattat WS, El-Sheshtawy RI, El-Batawy KA, Shahba MI, El-Seadawy IE (2016) Preservability of buffalo bull semen in tris-citrate extender enriched with bee’s honey. J Innov Pharm Biol Sci 3(1): 180-185.
El-Sheshtawy RI, El-Badry DA, Gamal A, El-Nattat WS, Almaaty AMA (2016) Natural honey as a cryoprotectant to improve Arab stallion post-thawing sperm parameters. Asian Pac J Reprod 5(4): 331-334.
El-Sheshtawy RI, El-Nattat WS, Sabra HA, Ali AH (2014) Effect of honey solution on semen preservability of local breeds of cattle bulls. Wld Appl Sci J 32(10): 2076-2078.
Fakhrildin MBM, Alsaadi RA (2014) Honey Supplementation to semen-freezing medium improveshuman sperm parameters post-thawing. J Family Reprod Health 8(1): 27.
Fuller BJ (2004) Cryoprotectants: the essential antifreezes to protect life in the frozen state. Cryo Letters 25(6): 375-388.
Gillan L, Evans G, Maxwell, WMC (2005) Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 63(2): 445-457.
Jafaroghli M, Khalili B, Farshad A, Zamiri MJ (2011) The effect of supplementation of cryopreservation diluents with sugars on the post-thawing fertility of ram semen. Small Rumin Res 96(1): 58-63.
Jerez-Ebensperger RA, Luno V, Olaciregui M, Gonzalez N, de Blas I, Gil L (2015) Effect of pasteurized egg yolk and rosemary honey supplementation on quality of cryopreserved ram semen. Small Rumin Res 130: 153-156.
Kwakman, PH, Zaat SA (2012) Antibacterial components of honey. IUBMB life 64(1):48-55.
Maidin MS, Padlan MH, Azuan SAN, Jonit R, Mohammed NH, Abdullah R (2018) Supplementation of Nigella sativa oil and honey prolong the survival rate of fresh and post-thawed goat sperms. Trop Anim Sci J 41(2): 94-99.
Marco-Jiménez F, Puchades S, Gadea J, Vicente, JS, Viudes-de-Castro MP (2005) Effect of semen collection method on pre-and post-thaw Guirra ram spermatozoa. Theriogenology 64(8): 1756-1765.
Medeiros CMO, Forell F, Oliveira ATD, Rodrigues JL (2002) Current status of sperm cryopreservation: why isn't it better?. Theriogenology 57(1): 327-344.
Mehdipour M, Kia HD, Nazari M, Najafi A (2017) Effect of lecithin nanoliposome or soybean lecithin supplemented by pomegranate extract on post-thaw flow cytometric, microscopic, and oxidative parameters in ram semen. Cryobiology 78: 34-40.
Motamedi‐Mojdehi R, Roostaei‐Ali Mehr M, Rajabi‐Toustani R (2014) Effect of different levels of glycerol and cholesterol‐loaded cyclodextrin on cryosurvival of ram spermatozoa. Reprod Domest Anim 49(1): 65-70.
Ögretmen F, İnanan BE (2014) Evaluation of cryoprotective effect of turkish pine honey on common carp (Cyprinus Carpio) Spermatozoa. Cryo Letters 35(5): 427-437.
Olayemi FO, Adeniji DA, Oyeyemi MO (2011) Evaluation of sperm motility and viability in honey-included egg yolk based extenders. Global Vet 7(1): 19-21.
Ouchemoukh S, Louaileche H, Schweitzer P (2007) Physicochemical characteristics, and pollen spectrum of some Algerian honeys. Food Control 18(1): 52-58.
Özkök A, Sorkun K, Salih B (2016) The microscopic and GC-MS analysis of Turkish pine honey. J Biol & Chem 44(4): 375-383.
Özmen MF, Cirit Ü, Arıcı R, Demir K, Kurt D, Pabuccuoğlu S et al. (2020) Evaluation of synergic effects of iodixanol and trehalose on cryosurvival of electroejaculated ram semen. Andrologia 52(9): e13656.
Öztürkler Y, Baran A, Evecen M, Ak K, İleri İK (2001) Comparison of ovine spermatozoal morphological features after staining of fixation and assessment of morphological abnormalities in dead/live spermatozoa. Turk J Vet Anim Sci 25(5): 675-680.
Panyaboriban S, Suwimonteerabutr J, Phutikanit N, Swangchan-Uthai T, Tharasanit T, Techakumphu M (2015) Effect of various combinations of sugar supplementation in the extender on frozen-thawed ram semen quality and fertility. Thai J Vet Med 45(2): 229.
Pelufo V, Armengol ML, Malcotti V, Venturino A, Aisen EG (2015) Effects of glycerol and sugar mixing temperature on the morphologic and functional integrity of cryopreserved ram sperm. Theriogenology 83(1): 144-151.
Salamon S, Maxwell WM (2000) Storage of ram semen. Anim Reprod Sci 62: 77–111.
Valente SS, Pereira RM, Baptista MC, Marques CC, Vasques MI, Pereira MS et al. (2010) In vitro and in vivo fertility of ram semen cryopreserved in different extenders. Anim Reprod Sci 117(1-2): 74-77.
Watson PF (2000) The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 60: 481-492.
Yimer N, Muhammad N, Sarsaifi K, Rosnina Y, Wahid H, Khumran AM et al. (2015) Effect of honey supplementation into Tris Extender on Cryopreservation of Bull Spermatozoa. Mal J Anim Sci 18(2): 47-54.