Immunohistochemical Demonstration of Mast Cells in Bovine Papillomatous Digital Dermatitis of Holstein-Friesian Dairy Cattle

PDF
Published: Jan 11, 2024
DOI: https://doi.org/10.12681/jhvms.29927
Keywords:
Bovine papillomatous Digital dermatitis Holstein-Friesian cattle Immunohistochemistry
CG Ozguden-Akkoc
Bursa Uludag University, Faculty of Veterinary Medicine, Department of Histology & Embryology, Bursa, Turkey
https://orcid.org/0000-0002-5090-7917
A Akkoç
Bursa Uludag University, Faculty of Veterinary Medicine, Department of Pathology, Bursa, Turkey
Abstract

Mast cells (MC)are unique members of immune system; their location and functions are of great importance in health and disease status. This study aims to evaluate MCs distribution and heterogenity in healthy and digital dermatitis lesions of dairy cattle for the first time. A total of 50 skin samples, 25 healthy and 25 with digital dermatitis (DD) lesions were sampled in Holstein-Friesian dairy cattle. All samples were stained with hematoxylin-eosin (H&E) for microscopic examination and also stained with Toluidine blue for MCs demonstration. Epidermal acanthosis and hyperkeratosis with dermal inflammatory cell infiltrations were in consistent with digital dermatitis lesions. Spirochetal agents were successfully demonstrated with Warthin-Starry staining in 22 out of 25 DD samples. Increased number of mast cell were observed in digital dermatitis samples when compared with healthy skin samples. The average number of intact MCs were 4.6 ± 2 and 9.3 ± 1 in healthy and digital dermatitis samples, respectively. Two-fold increase in the number of intact MCs in digital dermatitis samples was observed. Degranulated mast cell numbers in tissue sections were also higher in digital dermatitis samples and 4.25-fold increase was recorded in affected skin samples. Immunophenotype of MCs in skin samples were identified by immmunohistochemical stainings with anti-tryptase and chymase antibodies. Only tryptase positive MCs were observed in healthy and DD samples. In the statistical analysis, differences in the mean intact and degranulated MCs were found to be significant (mean intact: p<0.05, mean degranulated: p<0.01). Our results possibly suggest that MCs may have important roles in the pathogenesis of bovine digital dermatitis.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Bradding P (2009)Human lung mast cell heterogeneity.Thorax64:278-280.
Caddey B, De Buck J (2021)Meta-analysis of bovine digital dermatitis
microbiota reveals distinct microbial community structures associated with lesions.Front Cell Infect Microbiol 16:685-861.
Cairns JA, Walls AF (1996) Mast cell tryptase is a mitogen for epithelial
cells. Stimulation of IL-8 production and intercellular adhesion molecule-1 expression. J Immunol 156:275-283.
Caughey GH (2007)Mast cell tryptases and chymases in inflammation and
host defense.Immunol Rev 217:141-154.
CheliR,Mortellaro C (1974) La dermatite digitale del bovino. In: 8th International Meeting on Diseases of Cattle, Milan, Italy: pp 208-213.
Di Nardo A, Yamasaki K, Dorschner RA, Lai Y, Gallo RL (2008) Mast
cell cathelicidin antimicrobial peptide prevents invasive group A
Streptococcus infection of the skin.J Immunol180:7565-7573.
Elliott MK, Alt DP (2009) Bovine immune response to papillomatous
digital dermatitis (PDD)-associated spirochetes is skewed in isolate reactivity and subclass elicitation.Vet Immunol Immunopathol
:256-261.
Evans NJ, Brown JM,Demirkan I, Murray RD, Birtles RJ, Hart CA, Carter
SD (2009) Treponema pedis sp.nov., a spirochaeta isolated from bovine digital dermatitis lesions. Int J Syst Evol Microbiol59:987-991.
Evans NJ, Brown JM, Scholey R, Murray RD, Birtles RJ, Hart CA, Carter SD (2014)Differential inflammatory responses of bovine foot skin
fibroblasts and keratinocytes to digital dermatitis treponemes.Vet Immunol Immunopathol 161:12-20.
Evans NJ, Murray RD, Carter SD (2015) Bovine digital dermatitis: Current concepts from laboratory to farm. Vet J211:3-13.
Gomez A, Cook NB, Bernardoni ND, Rieman J, Dusick AF, Hartshorn
R, Socha MT, Read DH, Döpfer D (2012) An experimental infection model to induce digital dermatitis infection in cattle.J Dairy
Sci95:1821-1830.
Görgül OS, Kahraman MM,Çeçen G, Akkoç A, Gül NY, Sevimli A (2002)
Sığırlarda digital ve interdigital dermatitis’lerdekliniktanı, sağaltımvehistopatolojik bulgular. J Res Vet Med21:115-124.
Hsieh FH, Sharma P,Gibbons A, Goggans T, Erzurum SC, Haque SJ
(2005) Human airway epithelial cell determinants of survival and
functional phenotype for primary human mast cells.Proc Natl Acad
Sci USA102:14380-14385.
Holzhauer M, Hardenberg C, Bartels CJ, Frankena KJ (2006)Herdand cow- level prevalence of digital dermatitis in the Netherlands and
associated risk factors. Dairy Sci89:580-588.
Huang C, De Sanctis GT, O’Brien PJ, Mizgerd JP, Friend DS, Drazen JM,
Brass LF, Stevens RL (2001) Evaluation of the substrate specificity of
human mast cell tryptase beta I and demonstration of its importance
in bacterial infections of the lung.J Biol Chem276:26276-26284.
Klitgaard K, Foix Bretó A, Boye M, Jensen TK (2013) Targeting the
treponemal microbiome of digital dermatitis infections by high-resolution phylogenetic analyses and comparison with fluorescent in situ
hybridization. J Clin Microbiol51:2212-2219.
Knight PA, Wright SH, Lawrence CE, Paterson YY, Miller HR(2000) Delayed expulsion of the nematode Trichinella spiralis in mice lacking
the mucosal mast cell-specific granule chymase, mouse mast cell protease-1.J Exp Med192:1849-1856.
Küther K, Audigé L, Kube P, Welle M (1998) Bovine mast cells: distribution, density, heterogeneity, and influence of fixation techniques. Cell
Tissue Res293:111-119.
Lima HG, Pinke KH, Gardizani TP, Souza-Júnior DA, Carlos D, Avila-Campos MJ, Lara VS (2013) Mast cells act as phagocytes against
the periodontopathogen Aggregatibacter Actinomycetemcomitans.J
Periodontol84:265-272.
Losinger WCJ (2006)Economic impacts of reduced milk production associated with papillomatous digital dermatitis in dairy cows in the USA.
Dairy Res73:244-256.
Marks RM, Roche WR, Czerniecki M, Penny R, Nelson DS (1986) Mast
cell granules cause proliferation of human microvascular endothelial
cells.Lab Invest 55:289-294.
Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC
(2000)Human endothelial cells regulate survival and proliferation of
human mast cells.J Exp Med192:801-811.
Moe KK, Yano T, Misumi K,Kubato C, Yamazaki W, Muguruma M, Misawa N (2010) Analysis of the IgG immune response to Treponema
phagedenis-like spirochetes in individual dairy cattle with papillomatous digital dermatitis.Clin Vaccine Immunol17:376-83.
Mukai K, Tsai M, Saito H, Galli SJ (2018) Mast cells as sources of cytokines, chemokines, and growth factors.Immunol Rev282:121-150.
Nakamura Y, Oscherwitz J, Cease KB (2013)Staphylococcus δ-toxin induces allergic skin disease by activating mast cells.Nature503:397-401.
Orsel K, Plummer P, Shearer J, De Buck J, Carter SD, Guatteo R, Barkema HW (2018) Missing pieces of the puzzle to effectively control
digital dermatitis.TransboundEmerg Dis65:186-198.
Refaai W, Ducatelle R, Geldhof P, Mihi B, El-shair M, Opsomer G (2013)
Digital dermatitis in cattle is associated with an excessive innate immune response triggered by the keratinocytes.BMC Vet Res9:193.
Rozniecki JJ, Hauser SL, Stein M, Lincoln R, Theoharides TC (1995)
Elevated mast cell tryptase in cerebrospinal fluid of multiple sclerosis
patients. Ann Neurol37:63-66.
Siebenhaar F, Syska W, Weller K, Magerl M, Zuberbier T, Metz M, Maurer M (2007) Control of Pseudomonas aeruginosa skin infections in
mice is mast cell-dependent. Am J Pathol170:1910-1916.
Warthin AS, Chronister AC (1920) A more rapid and improved method
of demonstrating spirochetes in tissues (Warthin and Starry’s cover-glass method). AmJSyp4:97-103.
Zimmermann C, Troeltzsch D, Giménez-Rivera VA, Galli SJ, Metz M,
Maurer M, Siebenhaar F (2019)Mast cells are critical for controlling
the bacterial burden and the healing of infected wounds. Proc Natl
Acad Sci USA116:20500-20504.
Zuerner RL, Heidari M, Elliott MK, Alt DP, Neill JD (2007) Papillomatous digital dermatitis spirochetes suppress the bovine macrophage
Most read articles by the same author(s)