Border disease virus and chlamydophila abortus co-infection in aborted sheep foetuses


Published: Oct 31, 2023
Updated: 2023-10-31
Versions:
2023-10-31 (2)
Keywords:
Border disease virus chlamydophila abortus sheep abortion
M Şevik
https://orcid.org/0000-0002-9604-3341
Abstract

Abortion in sheep and goats is one of the most important reproductive problems that affect small ruminants breeding. Non-infectious factors and infectious agents such as bacterial, viral, mycotic and parasitic infectious agents can cause abortion in sheep and goats. A high rate of abortion (43.75%) was observed in two sheep flocks in Niğde Province in Turkey during the lambing season in 2016. To determine the reason of abortion, aborted sheep foetuses (n = 3) and EDTA whole blood samples (n = 3) from mother of the foetuses were collected from two sheep flocks. Aborted foetuses and buffy coat cells of the EDTA whole blood samples were used for total nucleic acid extraction. Extracted nucleic acids were analysed for akabane virus (AKAV), bluetongue virus (BTV), peste des petits ruminants virus (PPRV), pestiviruses, Schmallenberg virus (SBV), Brucella spp., Chlamydophila abortus (C. abortus), Coxiella burnetii and Listeria monocytogenes. Border disease virus (BDV) RNA and C. abortus DNA were detected in three aborted sheep foetuses whereas other investigated infectious agents were not detected. Additionally, EDTA whole blood samples from mother of the foetuses were also found BDV positive. To the best of my knowledge, this is the first report on BDV and C. abortus co-infection in aborted sheep foetuses.

Article Details
  • Section
  • Research Articles
Downloads
Download data is not yet available.
References
Albayrak H, Gumusova SO, Ozan E, Yazici Z (2012) Molecular detection
of pestiviruses in aborted foetuses from provinces in northern Turkey.
Trop Anim Health Prod 44(4):677-680.
Alemayehu G, Mamo G, Alemu B, Desta H, Tadesse B, Benti T, Bahiru A,
Yimana M, Wieland B (2021) Causes and Flock Level Risk Factors
of Sheep and Goat Abortion in Three Agroecology Zones in Ethiopia.
Front Vet Sci 29;8:615310.
Barati S, Bakhtiari NM, Shokoohizadeh L, Ghorbanpoor M, Momtaz H
(2022) Genotyping of Chlamydia abortus using multiple loci variable
number of tandem repeats analysis technique. BMC Vet Res 18(1):54.
Batten CA, Banyard AC, King DP, Henstock MR, Edwards L, Sanders A,
Buczkowski H, Oura CC, Barrett T (2011) A real time RT-PCR assay
for the specific detection of Peste des petits ruminants virus. J Virol
Methods 171(2):401-404.
Bilk S, Schulze C, Fischer M, Beer M, Hlinak A, Hoffmann B (2012)
Organ distribution of Schmallenberg virus RNA in malformed new
borns. Vet Microbiol 159:236-238.
Bricker BJ, Halling SM (1994) Differentiation of brucella abortus bv. 1,
, and 4, brucella melitensis, brucella ovis, and brucella suis bv. 1 by
PCR. J Clin Microbiol 32:2660-2666.
Brom Rvd, Santman-Berends I, Dijkman R, Vellema P, Dijkman R, Enge
len EV (2021) An Accessible Diagnostic Toolbox to Detect Bacterial
Causes of Ovine and Caprine Abortion. Pathogens 10(9):1147.
Clune T, Beetson S, Besier S, Knowles G, Paskin R, Rawlin G, Suter
R, Jacobson C (2021) Ovine abortion and stillbirth investigations in
Australia. Aust Vet J 99: 72-78.
Cokcaliskan C (2002) Gebe koyunlar ve fötuslarında Pestivirus enfeksiy
onu. PhD thesis, Ankara University Health Science Institute, Ankara.
GalVmed (2022) Chlamydia Abortus. https://www.galvmed.org/live
stock-and-diseases/livestock-diseases/chlamydia
abortus/#:~:tex
t=Abortion%20rates%20can%20reach%2040,and%20affect%20
early%20lamb%20development [accessed 14 June 2022].
Givens MD, Marley MS (2008) Infectious causes of embryonic and fetal
mortality. Theriogenology 70(3):270-285.
Güler L, Hadimli HH, Erganiş O, Ateş M, Ok U, Gündüz K (2006) Field
evaluation of a PCR for the diagnosis of chlamydial abortion in
sheep. Vet Rec 159(22):742-745.
Hasircioglu S, Kale M, Acar A (2009) Investigation of Pestivirus Infec
tions in Aborted Sheep and Goats in Burdur Region. Kafkas Univ Vet
Fak Derg 15(2):163-167.
Hofmann M, Griot C, Chaignat V, Perler L, Thür B (2008) Bluetongue
disease reaches Switzerland. Schweiz Arch Tierheilkd 150:49-56.
Kalender H, Kılış A, Eröksüz H, Muz A, Kılınç Ü, Taşdemir B (2013)
Identification of Chlamydophila abortus infection in aborting ewes and goats in Eastern Turkey. Revue Med Vet 164(6):295-301.
Klee SR, Tyczka J, Ellerbrok H, Franz T, Linke S, Baljer G, Appel B
(2006) Highly sensitive real-time PCR for specific detection and
quantification of Coxiella burnetii. BMC Microbiol 6:2.
Longbottom D, Livingstone M, Maley S, van der Zon A, Rocchi M, Wil
son K, Wheelhouse N, Dagleish M, Aitchison K, Wattegedera S, Nath
M, Entrican G, Buxton D (2013) Intranasal infection with Chlamydia
abortus induces dose-dependent latency and abortion in sheep. PLoS
One 8(2):e57950.
Menzies PI (2007) Abortion in sheep: diagnosis and control. In: Current
therapy in large animal theriogenology. WB Saunders, Missouri:pp
-680.
Menzies PI (2011) Control of important causes of infectious abortion in
sheep and goats. Vet Clin North Am Food Anim Pract 27(1):81-93.
Nettleton PF, Gilray JA, Russo P, Dlissi E (1998) Border disease of sheep
and goats. Vet Res 29:327-340.
OIE (2017) Border Disease. http:// www.oie.int/fileadmin/Home/eng/
Health_standards/ tahm/2.07.01_BORDER_DIS.pdf [accessed 14
June 2022].
OIE (2018) Enzootic abortion of ewes (Ovine Chlamydiosis) https://
www.woah.org/fileadmin/Home/fr/Health_standards/tahm/3.07.05_
ENZ_ABOR.pdf [accessed 14 June 2022].
Rossmanith P, Krassnig M, Wagner M, Hein I (2006) Detection of Listeria
monocytogenes in food using a combined enrichment/real-time PCR
method targeting the prfA gene. Res Microbiol 157:763-771.
Stram Y, Kuznetzova L, Guini M, Rogel A, Meirom R, Chai D, Yadin H,
Brenner J (2004) Detection and quantitation of akabane and aino vi
ruses by multiplex realtime reverse-transcriptase PCR. J Virol Meth
ods 116:147-154.
Şevik M (2018) The Role of Pestiviruses (BDV and BVDV) in Ruminant
Abortion Cases in the Afyonkarahisar Province. Kocatepe Vet J
(3):238-244.
Şevik M (2021) Genomic characterization of pestiviruses isolated from
bovine, ovine and caprine foetuses in Turkey: A potentially new
genotype of Pestivirus I species. Transbound Emerg Dis 68(2):417
Thiele D, Wittenbrink MM, Fischer D, Krauss H (1992) Evaluation of the
polymerase chain reaction (PCR) for detection of Chlamydia psittaci
in abortion material from ewes. Zentralbl Bakteriol 277(4):446-453.
Tuncer-Göktuna P, Alpay G, Öner EB, Yeşilbağ K (2016) The role of
herpesviruses (BoHV-1 and BoHV-4) and pestiviruses (BVDV and
BDV) in ruminant abortion cases in western Turkey. Trop Anim
Health Prod 48(5):1021-1027.
Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ
(1994) Pestiviruses isolated from pigs, cattle and sheep can be allo
cated into at least three genogroups using polymerase chain reaction
and restriction endonuclease analysis. Arch Virol 136(3-4):309-323.
Most read articles by the same author(s)