Establishment of AA-NAT, SCD, PROP1, and IGFBP3 gene frequency distributions in Karacabey Merino and Kivircik Sheep Breeds
Published:
Oct 18, 2023
Keywords:
Karacabey Merino Kivircik AA-NAT SCD PROP1 IGFBP3
Abstract
Thus, the aim of the current study was to investigate the genotypic dispersion of AA-NAT, SCD, PROP1, and IGFBP3 genes in the target population. Primarily, the genomic DNA was isolated from blood samples by the phenol/chloroform method. The genomic analyses were performed on a total of 160 Karacabey Merino (KM) sheep (n=80) and Kivircik (n=80). The PCR-RFLP method was used to determine the genotype of the SNPs. Deviation from Hardy–Weinberg equilibrium (HWE) and population genetic assays such as observed (Ho) and expected (He) heterozygosities, effective allele numbers (Ne), and the polymorphism information content (PIC) were calculated for each gene in the population. As a result, there was no deviation from HWE was determined except SCD and PROP1 gene in KM. The most frequent genotypes were found AA (66,25% and 87,50%) for AA-NAT gene, CC (93,75% and 91,25%) for SCD gene and CC genotype (70,00% and 56,25%) for PROP1 gene in KM and Kivircik sheep. All breeds were detected monomorphic in terms of the IGFBP3 gene. In conclusion, the other regions of the IGFBP3 gene which were determined as monomorphic in the studied flock could be researched. Moreover, further studies should be necessary for defining the effects of investigated genes on related traits in KM and Kivircik sheep in terms of polymorphic genes specified.
Article Details
- How to Cite
-
Dinçel, D., & Çobanoğlu, Ö. (2023). Establishment of AA-NAT, SCD, PROP1, and IGFBP3 gene frequency distributions in Karacabey Merino and Kivircik Sheep Breeds. Journal of the Hellenic Veterinary Medical Society, 74(3), 6003–6012. https://doi.org/10.12681/jhvms.30666
- Issue
- Vol. 74 No. 3 (2023)
- Section
- Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
Downloads
Download data is not yet available.
References
Aali M, Moradi-Shahrbabak H, Moradi-Shahrbabak M, Sadeghi M,
Kohram H (2016) Polymorphism in the SCD gene is associated with
meat quality and fatty acid composition in Iranian fat- and thin-tailed
sheep breeds. Livest Sci 188:81-90.
Aali M, Shahrbabak MM, H. Shahrbabak H, Sadeghi M (2014) Identify
ing novel SNPs and allelic sequences of the stearoyl-Coa desaturase
gene (SCD) in fat-tailed and thin-tailed sheep breeds. Biochem. Gen
et. 52(3-4):153-158.
Addin GN, Mohammed TR, Al-Anbari NN (2016) Polymorphism of AA
NAT gene and its relationship with the productive and reproductive
performance in Turkish Awassi ewes. Anbar Journal of Agricultural
Sciences, 14(2), 246-255.
Ali BA, EL-Hanafy AA, Salem HH (2009) Genetıc Biodiversity Studies
On IGFBP-3 Gene in Egyptian Sheep Breeds. Biotechnol Anim Husb
(1-2): 101-109.
AL-Khuzai HMH, AL-Anbari NN (2019) Relationship of IGFBP-3 gene
Polymorphism with some of economical traits in Iraqi Awassi Ewes.
MJA,47(III): 174-180.
Bakhtiarizadeh M, Moradi-Shahrbabak M, Ebrahimie E (2014) Transcrip
tional regulatory network analysis of the over-expressed genes in ad
ipose tissue. Genes Genom 36(1):105-117.
Bernard L, Leroux C, Hayes H, Gautier M, Chilliard Y, Martin P (2001)
Characterization of the caprine stearoyl-CoA desaturase gene and its
mRNA showing an unusually long 3’-UTR sequence arising from a
single exon. Gene 281(1-2):53-61.
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a
genetic linkage map in man using restriction fragment length poly
morphisms, Am. J. Hum. Genet 32, 314-331.
Bozhilova-Sakova M, Dimitrova I, Petrov N (2019) Investigation of poly
morphisms on ABCG2 and AA-NAT genes in different sheep breeds
in Bulgaria. Bulg. J. Agric. Sci., 25 (Suppl. 1).
Calvo JH, González-Calvo L, Dervishi E, Blanco M, Iguácel LP, Sarto P,
Pérez-Campo FM, Serrano M, Bolado-Carrancio A, Rodríguez-Rey
JC, Joy M (2019) A functional variant in the stearoyl-CoA desaturase
(SCD) gene promoter affects gene expression in ovine muscle, Livest
Sci 219: 62-70.
Castro LFC, Wilson JM, Goncalves O, Galante-Oliveira S, Rocha E,
Cunha I (2011) The evolutionary history of the stearoyl-CoA desatu
rase gene family in vertebrates. BMC Evol Biol 11(1): 132.
Choudhary V, Kumar P, Saxena V, Bhattacharya TK, Bhushan B, Shar
ma A, Ahmed KA (2006) Effect of Leptin and IGFBP-3 Gene Poly
morphisms on Serum IgG Level of Cattle Calves. Anim Biosci
(8):1095-1099.
Ding-Ping B, Cheng-Jiang Y, Yu-Lin C (2012) Association between AA
NAT gene polymorphism and reproductive performance in sheep.
Electron. J. Biotechnol 15(2): 1-7.
Dobrzyn A, Ntambi JM (2005) The role of stearoyl-CoA desaturase in the
control of metabolism. Prostaglandins Leukot. Essent. Fatty Acids,
(1): 35-41.
Dupre SM, Burt DW, Talbot R, Downing A, Mouzaki D, Waddington D,
Malpaux B, Davis JR, Lincoln GA, Loudon AS (2008) Identification
of melatonin-regulated genes in the ovine pars tuberalis, a target site
for seasonal hormonal control. Endocrinology 149: 5527-5539.
Ekegbu UJ, Burrows L, Amirpour-Najafabadi H, Zhou H, Hickford J
(2019) Gene polymorphisms in PROP1 associated with growth traits
in sheep. Gene 683: 41-46.
EL-Hanafy Amr A, Salem HH (2009) PCR-RFLP of IGFBP-3 Gene in
Some Egyptian Sheep Breeds. J Agric & Environ Sci 5(1): 82-85.
Falk A (2013) Variation in frequency of alleles in the MTNR1A gene with
possible impact on ability of ewes to show oestrus out of season, Mas
ter thesis, Swedish University of Agricultural Science, Uppsala.
Fathy HA, Gouda EM, Gafer JA, Galal MK, Nowier AM (2018) Genetic
polymorphism in melatonin receptor 1A and arylalkylamine N-acet
yltransferase and its impact on seasonal reproduction in Egyptian
sheep breeds. Arch Anim Breed 61(4): 505-516.
Garcia-Fernandez M, Gutierrez-Gil B, Garcia-Gamez E, Sanchez JP, Ar
ranz JJ (2010) Detection of quantitative trait loci affecting the milk
fatty acid profile on sheep chromosome 22: Role of the stearoyl-CoA
desaturase gene in Spanish Churra sheep. J Dairy Sci 93(1):348-357.
Green M, Sambrook J (2012) Analysis of DNA. In: Molecular Cloning:
A Laboratory Manual. 4th ed, Cold Spring Harbor Laboratory Press,
New York: pp 350-370.
Hirwa C, Wallace P, Shen X, Nie Q, Yang G, Zhang X (2011) Genes re
lated to economically important traits in beef cattle. Asian J Anim
Sci 5: 34-45.
10.2021].
Izadi M, Naserian A, Nasiri M, Heravi R (2014) An Evolutionary Rela
tionship between Stearoyl-CoA Desaturase (SCD) Protein Sequences
Involved in Fatty Acid Metabolism. Rep Biochem Mol Biol 3(1): 1-6.
Klein DC, Berg GR (1970) Pineal gland: Stimulation of melatonin produc
tion by norepinephrine involves cyclic AMP-mediated stimulation of
N-acetyltransferase. Adv. Biochem. Psychopharmacol 3: 241-263.
Koyuncu M, Kara Uzun S (2009) Growth performance of Karacabey Me
rino and Kivircik lambs under semi-intensive management in Turkey.
Small Rumin Res 83: 64-66.
Kuchel H, Siebert BD, Bottema CDK, Webb GC, Crawford AM, Dun
can SJ, McDonald PA, McEwan JC, Pitchford WS (2004) Physical
mapping of the stearoyl-CoA desaturase (SCD) locus in sheep. Anim
Genet 35:163.
Kumar P, Choudhary V, Ganesh Kumar K, Bhattacharya TK, Bhushan B,
Sharma A, Mishra A (2006) Nucleotide sequencing and DNA poly
morphism studies on IGFBP-3 gene in sheep and its comparison with
cattle and buffalo. Small Rumin Res 64: 285-292.
Lincoln GA (2006) Decoding the nightly melatonin signal through circa
dian clockwork. Mol. Cell. Endocrinol 252: 69-73.
Liu Q, Geng C, Chu M, Chen H, JinM, Zhang Y, Di R, Feng T, Li N
(2015) Polymorphism of Prophet of Pit-1 gene and its relationship
with litter size of Small Tail Han sheep. J Genet 94: e27-e30.
Maciulla JH, Zhang HM, Denise SK (1997) A novel polymorphism in the
bovine insuline-like growth factor binding protein -3 (IGFBP3) gene.
Anim Genet 28:370-383.
Mahrous KF, Hassanane MS, Abdel Mordy M, Shafey HI, Rushdi HE
(2015) Polymorphism of some Genes Associated with Meat‐Related
Traits in Egyptian Sheep Breeds. Iran J Appl Anim Sci 5(3), 655-663.
Ming-xing C, Yan Y, Ping-qing W, Guligena Huiguo Y, Geng H, Jianguo
Y, Qianqian T, Tao F, Guiling C, Dongwei H, Ran D, Qiuyue L, Ning
L (2013) Polymorphism of AA-NAT gene and its relationship with
litter size of Jining Grey goat of China. Anim Sci Pap 31: 15-26.
Ntambi JM (1999) Regulation of stearoyl-CoA desaturase by polyunsatu
rated fatty acids and cholesterol. J Lipid Res 40: 1549-1558.
Öner Y, Orman A, Üstüner A, Yilmaz A (2014) Investigation of Poly
morphisms on ABCG2, AA-NAT and FABP3 Genes in the Kıvırcık
Sheep Reared in Three Different Provinces of Turkey. Kafkas Univ
Vet Fak Derg 20(5): 649-654.
Padma B, Kumar P, Choudhary V, Dhara SK, Mishra A, Bhattacharya TK,
Bhushan B, Sharma A (2004) Nucleotide sequencing and PCR-RFLP
of insulin-like growth factor binding protein-3 gene in riverine buffa
lo (Bubalus bubalis). Asian-Aust J Anim Sci 17: 910-913.
Quiñones J, Bravo S, Calvo JH, Sepúlveda N (2017) Genetic polymor
phism in meat fatty acids in araucano creole sheeps. J Anim Plant Sci
(3): 743-746.
Rajashekhara S, Pawar VD, Sawane MP, Deshmukh RS, Komatwar SJ,
Ingle SA (2018) PCR-RFLP of IGFBP-3 Gene in Madgyal Sheep. Int.
J. Sci. Environ. Technol. 7(3): 801-808.
Reardon W, A Mullen, Sweeney T, Hamill R (2010) Association of poly
morphisms in candidate genes with colour, water-holding capacity,
and composition traits in bovine M. longissimus and M. semimem
branosus. Meat Sci 86(2): 270-275.
Rosa H, Bryant M (2003) Seasonality of reproduction in sheep, Small
Rumin Res 48: 155-171.
Saleh AA, Rashad AMA, Hassanine NNAM et al (2019) Comparative
analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and
buffalo. BMC Res Notes 12: 623.
Shafey HI, Mahrous KF, Hassanane MS, Abdel Mordy M, Rushdi HE
(2014) Genetic Polymorphism of Myostatin and Insulin-Like Growth
Factor Binding Protein-3 Genes in Egyptian Sheep Breeds. Glob Vet
(3): 419-424.
Sharma B, Dass G, Sharma D (2011) PCR-RFLP in IGFBP-3 Gene and its
Association with Economic Traits in Muzaffarnagari Sheep. Interna
tional Conference on Chemical, Biological and Environment Scienc
es (ICCEBS’2011) Bangkok.
Vesper AH, Raetzman LT, Camper, SA (2006) Role of prophet of Pit1
(PROP1) in gonadotrope differentiation and puberty. Endocrinology
(4): 1654-1663.
Ward RJ, Travers MT, Richards SE, Vernon RG, Salter AM, Buttery PJ,
Barber, MC (1998) Stearoyl-CoA desaturase mRNA is transcribed
from a single gene in the ovine genome, Biochim Biophys Acta 1391:
-156.
Wayne NL, Malpaux B, Karsch, FJ (1988) How does melatonin code for
day length in the ewe: Duration of nocturnal melatonin release or
coincidence of melatonin with a light-entrained sensitive period? Biol
Reprod 39(1): 66-75.
Woelfle J, Chia DJ, Rotwein P (2003) Mechanisms of growth hormone
(GH) action identification of conserved STAT5 binding sites that me
diate GH-induced insulin-like growth factor-i gene activation. J Biol
Chem 278: 51261-51266.
Yeh F, Yang RC, Boyle T (2000) PopGene (v.1.32), Microsoft Win
dows-based freewarefor population genetic analysis. Retrieved from
Zeng X, Chen, H, Jia B, Zhao Z, Hui W, Zhang W, Wu H, Wang Z (2011)
Effects of single and combined genotypes of MC4R and PROP1 gene
on growth traits in Chinese Merino Sheep. Acta Vet et Zoo Sinica
(9): 1227-1232.
Zhang R, Chen H, Lei C, Fang XT, Zhang YD, Hu SR, Su LH (2007) As
sociation between PCR-RFLP Polymorphisms of Five Gene Loci and
Milk Traits in Chinese Holstein. Anim Biosci 20(2):166-171.
Zheng W, Scheibner KA, Ho AK, Cole PA (2001) Mechanistic studies on
the alkyltransferase activity of serotonin N-acetyltransferase. Chem
Biol 8(4), 379-389.
