The influence of various rates of royal jelly enriched extenders on frozen/thawed sperm quality and fertility in goats
Published:
Oct 18, 2023
Keywords:
Artificial Insemination Cryopreservation goat royal gelly semen extenders
Abstract
The goal of this study was to detect the influence of royal jelly which has a strong antioxidant capacity supplementation in TRIS egg yolk and TRIS soybean lecithin extenders on quality and fertility. Sperm was collected from 5 Damascus bucks via an electro ejaculator during the breeding season. After dilution and cooling, the lowest motility and membrane integrity were detected in TEY-1RJ, in terms of abnormal spermatozoon rate, the TEY-1RJ group was the highest in cooled semen (P<0.05). After dilution and cooling, osmolality decreased significantly when the RJ ratio increased (P<0.05). After freezing-thawing, in both TEY and TSL extenders, the lowest sperm quality was detected in the 1% RJ groups. In terms of intact acrosome ratio, the highest value was recorded in the 0.50% RJ group in TEY and TSL diluents (P<0.05). Fertility assessments were conducted with 150 goats synchronized with intravaginal sponges and goats were inseminated transcervical. There was no difference between pregnancy rates between TEY and TSL-control and RJ groups (P>0.05). As a result, it was deduced that RJ additions to TEY and TSL extenders did not produce any alternative to freezing on sperm quality in Damascus goat semen except for the intact acrosome ratio and had no positive effect on fertility.
Article Details
- How to Cite
-
Coskun Cetin, N., & Karaca, F. (2023). The influence of various rates of royal jelly enriched extenders on frozen/thawed sperm quality and fertility in goats. Journal of the Hellenic Veterinary Medical Society, 74(3), 6073–6084. https://doi.org/10.12681/jhvms.30798
- Issue
- Vol. 74 No. 3 (2023)
- Section
- Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
Downloads
Download data is not yet available.
References
Abd-Allah, S.M., 2010. Effect of royal jelly on bovine sperm characteris
tics during post-thaw incubation in vitro. Revista Veterinaria, 21(2):
-85.
Abd-Allah, S.M., 2012. Effect of royal jelly on the fertilizing ability of
buffalo spermatozoa in vitro. Journal of Buffalo Science, 1(1): 1-4.
Abdelhafiz, A.T. and Muhamad, J.A., 2008. Midcycle pericoital intravagi
nal bee honey and royal jelly for male factor infertility. Int J Gynaecol
Obstet, 101(2): 146-9.
Abdelnour, S.A. et al., 2020. Useful impacts of royal jelly on reproductive
sides, fertility rate and sperm traits of animals. J Anim Physiol Anim
Nutr (Berl), 104(6): 1798-1808.
Aboagla, E.M.-E. and Terada, T., 2004. Effects of egg yolk during the
freezing step of cryopreservation on the viability of goat spermato
zoa. Theriogenology, 62(6): 1160-1172.
Ahmed, M.M., El-Shazly, S.A., Alkafafy, M.E., Mohamed, A.A. and
Mousa, A.A., 2018. Protective potential of royal jelly against cadmi
um-induced infertility in male rats. Andrologia, 50(5): e12996.
Ahmed, M.M., Makawi, S.l. and Jubara, A., 1998. Synchronization of
oestrus in Nubian goats. Small Ruminant Research, 30(2): 113-120.
Akhter, S. et al., 2010. Cryopreservation of buffalo (Bubalus bubalis) se
men in Bioxcell® extender. Theriogenology, 74(6): 951-955.
Al-Faruque, M., Bari, F., Siddiqui, M. and Shamsuddin, M., 2007. Fer
tilizing capacity of buck (Capra hircus) semen frozen with different
concentrations of egg yolk. Journal of the Bangladesh Agricultural
University, 5(452-2018-3947): 95-104.
Al‐Merestani, M., Zarkawi, M. and Wardeh, M., 2003. Improving the
reproductive efficiency, pregnancy diagnosis and monitoring the re
sumption of luteal activity in indigenous Damascus goats. Reproduc
tion in domestic animals, 38(1): 36-40.
Alcay, S. et al., 2019. Successful cryopreservation of honey bee drone
spermatozoa with royal jelly supplemented extenders. Cryobiology,
: 28-31.
Alcay, S., Toker, M.B., Onder, N.T. and Gokce, E., 2017. Royal jelly
supplemented soybean lecithin-based extenders improve post-thaw
quality and incubation resilience of goat spermatozoa. Cryobiology,
: 81-85.
Amini, S. et al., 2019. Effects of supplementation of Tris-egg yolk extend
er with royal jelly on chilled and frozen-thawed ram semen character
istics. Cryobiology, 88: 75-80.
Arboud, M., Waheeb, R., Sheshtawy, R. and Amrawi, G., 2021. Effect of
Different Concentrations of Tris Royal Jelly-Enriched Extender on
Cooled and Post-Thawed Bull Semen. Alexandria Journal of Veter
inary Sciences, 68(1).
Atalla, H. et al., 2019. Impacts of supplementary royal jelly on charac
teristics of post-thawed ram semen. International Journal of Current
Research in Biosciences and Plant Biology, 6(1): 6-11.
Azad, F., Najati, V., Najafi, G. and Rahmani, F., 2018. The Protective
Effect of Royal Jelly on Testicular Tissue and Sperm Parameters in
Adult Mice Treated with Nicotine. Journal of Babol University of
Medical Sciences, 20(3): 50-58.
Bansal, A.K. and Bilaspuri, G., 2011. Impacts of oxidative stress and
antioxidants on semen functions. Veterinary medicine international,
Barbas, J. and Mascarenhas, R., 2009. Cryopreservation of domestic ani
mal sperm cells. Cell and tissue banking, 10(1): 49-62.
Baruah, D. et al., 2019. Effect of tris egg yolk and tris soya lecithin ex
tenders in frozen beetal buck semen. International Journal of Current
Microbiology and Applied Sciences, 8(1): 2740-2748.
Batista, M. et al., 2009. Successful artificial insemination using semen
frozen and stored by an ultrafreezer in the Majorera goat breed. Ther
iogenology, 71(8): 1307-1315.
Bergeron, A. and Manjunath, P., 2006. New insights towards understand
ing the mechanisms of sperm protection by egg yolk and milk. Mo
lecular reproduction and development, 73(10): 1338-1344.
Bhai, K.L. et al., 2015. Motility and functional membrane integrity of
buck spermatozoa with soyabean lecithin based extender. Journal of
J HELLENIC VET MED SOC 2023, 74 (3)
ΠΕΚΕ 2023, 74 (3)
N.COŞKUN ÇETIN, F. KARACA
Cell and Tissue Research, 15(1): 4711.
Bilodeau, J.-F., Blanchette, S., Gagnon, C. and Sirard, M.-A., 2001. Thiols
prevent H2O2-mediated loss of sperm motility in cryopreserved bull
semen. Theriogenology, 56(2): 275-286.
Bucak, M.N. and Tekin, N., 2007. Kryoprotektanlar ve gamet hücrelerinin
dondurulmasında kryoprotektif etki. Ankara Üniversitesi Veteriner
Fakültesi Dergisi, 54(1): 67-72.
Câmara, D. et al., 2011. Effects of reduced glutathione and catalase on the
kinematics and membrane functionality of sperm during liquid stor
age of ram semen. Small Ruminant Research, 100(1): 44-49.
Cetin, N.C., Kaan, O.K.O., Ates, C.T. and Karaca, F., 2020. Effect of roy
al jelly on quality of chilled semen from damascus buck. Journal of
Veterinary Andrology, 5(1).
Dorado, J., Rodriguez, I. and Hidalgo, M., 2007. Cryopreservation of goat
spermatozoa: Comparison of two freezing extenders based on post
thaw sperm quality and fertility rates after artificial insemination.
Theriogenology, 68(2): 168-177.
El-Sherbiny, A., 2013. Effect of royal jelly in honey-included egg yolk
based extenders on motility, viability and fertilizing ability of frozen
rabbit spermatozoa. Egyptian Journal of Rabbit Science, 23(2): 149
Elnagar, S.A., 2010. Royal jelly counteracts bucks’ “summer infertility”.
Anim Reprod Sci, 121(1-2): 174-80.
Elsheshtawy, R., 2020. Effect of Royal Jelly Tris-enriched Extender on
Buffalo Semen Cryopreservation. Egyptian Journal of Veterinary Sci
ences, 51(3): 405-411.
Eshtiyaghi, M., Deldar, H., Pirsaraei, Z.A. and Shohreh, B., 2016. Royal
jelly may improve the metabolism of glucose and redox state of ovine
oocytes matured in vitro and embryonic development following in
vitro fertilization. Theriogenology, 86(9): 2210-2221.
Fatet, A., Pellicer-Rubio, M.-T. and Leboeuf, B., 2011. Reproductive cy
cle of goats. Animal reproduction science, 124(3-4): 211-219.
Fathi, M. et al., 2019. Soybean lecithin-based extender improves Damas
cus goat sperm cryopreservation and fertilizing potential following
artificial insemination. Asian Pacific Journal of Reproduction, 8(4):
Forouzanfar, M. et al., 2010. In vitro comparison of egg yolk-based and
soybean lecithin-based extenders for cryopreservation of ram semen.
Theriogenology, 73(4): 480-487.
Gamal, A., El-Nattat, W.S., El-Sheshtawy, R.I. and El-Maaty, A.M.A.,
Substitution of egg yolk with different concentrations of soy
bean lecithin in tris-based extender during bulls’ semen preservabili
ty. Asian Pacific Journal of Reproduction, 5(6): 514-518.
Gawish, A.M. et al., 2016. Sperm abnormality toxicity due to cyclospo
rine A and the ameliorative effect of royal jelly in male rats. The Jour
nal of Basic & Applied Zoology, 76: 60-73.
Ghanbari, E., Nejati, V., Najafi, G., Khazaei, M. and Babaei, M., 2015.
Study on the effect of royal jelly on reproductive parameters in strep
tozotocin-induced diabetic rats. International journal of fertility &
sterility, 9(1): 113.
Gimenez-Diaz, C., Emsen, B., Emsen, E., Kutluca, M. and Koycegiz, F.,
Improved reproductive response of sheep in intrauterine in
semination program with the use of royal jelly. African Journal of
Biotechnology, 11(61): 12518-12521.
Guo, H. et al., 2008. Royal jelly peptides inhibit lipid peroxidation in vitro
and in vivo. Journal of nutritional science and vitaminology, 54(3):
-195.
Hadavand Mirzaei, A., Deldar, H., Ansari Pirsaraei, Z. and Shohreh, B.,
Royal jelly may improve sperm characteristics during preser
vation of rooster semen: Gene expression of antioxidant enzymes.
Reprod Domest Anim, 56(4): 658-666.
Hafez, E.S.E. and Hafez, B., 2013. Reproduction in farm animals. John
Wiley & Sons.
Iljenkaite, A. et al., 2020. The effect of royal jelly on boar sperm viability
and motility during liquid storage for 96 hours. Acta Veterinaria Brno,
(1): 47-53.
Jafari, A.Y. and Atarchi, H., 2008. The effect of royal jelly in tris extender
on sperm characteristics of Dallagh rams.
Jeyendran, R., Van der Ven, H., Perez-Pelaez, M., Crabo, B. and Zaneveld,
L., 1984. Development of an assay to assess the functional integrity
of the human sperm membrane and its relationship to other semen
characteristics. Reproduction, 70(1): 219-228.
Kajaysri, J. and Thammakarn, C., 2012. Estrus synchronization using
intravaginal medroxyprogesterone acetate (MAP), MAP plus pros
taglandin F2α, controlled internal drug release (CIDR) or CIDR plus
prostaglandin F2α in Saanen dairy goats in Thailand. Agriculture and
Natural Resources, 46(1): 71-79.
Kaleem, M., Rehman, A., Mehmood, U. and Sattar, M.A., 2017. Effects
of royal jelly on post thaw semen quality parameters of beetal buck.
Wayamba Journal of Animal Science: 1495 - 1498.
Khalifa, E., 2015. Physiological response of Zaraibi Billy goats spermato
zoa to soybean lecithin addition as an alternative to egg yolk varieties
in semen extenders. Egyptian Journal of Animal Production, 52(3):
-162.
Kharche, S. et al., 2013. Fertility following frozen semen artificial insem
ination in Jamunapari goats. Indian J. Anim. Sci, 83(10): 1071-1073.
Khazaei, M., Ansarian, A. and Ghanbari, E., 2018. New Findings on Bio
logical Actions and Clinical Applications of Royal Jelly: A Review. J
Diet Suppl, 15(5): 757-775.
Leboeuf, B., Restall, B. and Salamon, S., 2000. Production and storage of
goat semen for artificial insemination. Animal reproduction science,
(1-3): 113-141.
Mahdivand, N., Najafi, G., Nejati, V., Shalizar-Jalali, A. and Rahmani, F.,
Royal jelly protects male rats from heat stress-induced repro
ductive failure. Andrologia, 51(3): e13213.
Makawi, S.E.A., Salim, M.A.W., Adam, A.A.G. and Elsharif, B.A.A.,
Freezability and fertility of frozen-thawed kabashi eco-type
desert rams semen processed with tris-soybean and tris-egg yolk
based extenders. Journal of Veterinary Medicine and Animal Produc
tion, 4(2).
Martemucci, G. and D’Alessandro, A., 2011. Induction/synchronization
of oestrus and ovulation in dairy goats with different short term treat
ments and fixed time intrauterine or exocervical insemination system.
Animal reproduction science, 126(3-4): 187-194.
Masluchah, M. and Ducha, N., 2021. Pengaruh Penambahan Royal Jelly
dalam Pengencer Dasar Soya terhadap Kualitas Spermatozoa Kamb
ing Boer Sebelum dan Sesudah Ekuilibrasi. LenteraBio: Berkala Ilm
iah Biologi, 9(3): 218-225.
Masoudi, R. et al., 2016. Fertility and flow cytometry study of fro
zen-thawed sperm in cryopreservation medium supplemented with
soybean lecithin. Cryobiology, 73(1): 69-72.
Moghaddam, A., Karimi, I., Borji, M., Bahadori, S. and Abdolmoham
madi, A., 2013. Effect of royal jelly in ovo injection on embryon
ic growth, hatchability, and gonadotropin levels of pullet breeder
chicks. Theriogenology, 80(3): 193-8.
Mohammadian, T., Motlagh, M. and Shahneh, A., 2016. Effect of using
different levels of royal jelly in semen extender on some quality and
quantity parameters of Mahabadi goat semen. Iranian Journal of Ani
mal Science (IJAS), 46(4).
Molina, F.A.A., Pardo, B., Sánchez, M., Lopez, M. and Marín, C.C.P.,
Factors influencing the success of an artificial insemina
tion program in Florida goats. Spanish Journal of Agricultural Re
search(2): 338-344.
Moradi, A., Malekinejad, H., Farrokhi-Ardabili, F. and Bernousi, I., 2013.
Royal Jelly improves the sperm parameters of ram semen during liq
uid storage and serves as an antioxidant source. Small ruminant re
search, 113(2-3): 346-352.
Nunes, J. and Salgueiro, C., 2011. Strategies to improve the reproductive
efficiency of goats in Brazil. Small Ruminant Research, 98(1-3): 176
Nur, Z., Zik, B., Ustuner, B., Sagirkaya, H. and Ozguden, C., 2010. Ef
fects of different cryoprotective agents on ram sperm morphology and
DNAintegrity. Theriogenology, 73(9): 1267-1275.
Purdy, P., 2006. A review on goat sperm cryopreservation. Small ruminant
research, 63(3): 215-225.
Raeesi Dehkohneh, S., Mamouei, M., Tabatabaei, S., Fayazi, J. and
Naghibi Rokni, H., 2017. concentrations of Royal Jelly supplementa
tion in tris diluent on semen quality parameters of Arabi ram. Iranian
Veterinary Journal, 13(2): 13-21.
Saberivand, A., Pashapour, S., Noghani, A.E. and Namvar, Z., 2021. Synergistic effect of Royal jelly in combination with glycerol and dimeth
yl sulfoxide on cryoprotection of Romanov ram sperm. Cryobiology.
Salmani, H., Towhidi, A., Zhandi, M., Bahreini, M. and Sharafi, M., 2014.
In vitro assessment of soybean lecithin and egg yolk based diluents
for cryopreservation of goat semen. Cryobiology, 68(2): 276-280.
Sarıözkan, S. et al., 2010. Effects of different extenders and centrifuga
tion/washing on postthaw microscopic-oxidative stress parameters
and fertilizing ability of Angora buck sperm. Theriogenology, 73(3):
-323.
Shahzad, Q. et al., 2016. Royal jelly supplementation in semen extend
er enhances post-thaw quality and fertility of Nili-Ravi buffalo bull
sperm. Anim Reprod Sci, 167: 83-8.
Silici, S., Ekmekcioglu, O., Eraslan, G. and Demirtas, A., 2009. Antiox
idative effect of royal jelly in cisplatin-induced testes damage. Urol
ogy, 74(3): 545-51.
Sun, L. et al., 2020. Effect of substituting different concentrations of soy
bean lecithin and egg yolk in tris-based extender on goat semen cryo
preservation. Cryobiology, 92: 146-150.
Tekin, N., 1990. Erkek üreme organlarının muayenesi (Androlojik muay
eneler). Theriogenoloji evcil hayvanlarda reprodüksiyon sun’i tohu
mlama obstetrik ve infertilite. Nurol Matbacılık, Ankara: 53-67.
Toker, M.B., Alcay, S., Gokce, E. and Ustuner, B., 2016. Cryopreservation
of ram semen with antioxidant supplemented soybean lecithin-based
extenders and impacts on incubation resilience. Cryobiology, 72(3):
-209.
U
̈stüner, B., Alçay, S., Nur, Z., Sağırkaya, H. and Soylu, M.K., 2014.
Effect of egg yolk and soybean lecithin on tris-based extender in post
thaw ram semen quality and in vitro fertility. Kafkas Univ Vet Fak
Derg, 20(3): 393-398.
Valiollahpoor Amiri, M., Deldar, H. and Ansari Pirsaraei, Z., 2016. Impact
of supplementary royal jelly on in vitro maturation of sheep oocytes:
genes involved in apoptosis and embryonic development. Systems
biology in reproductive medicine, 62(1): 31-38.
Veshkini, A. et al., 2018. Oocyte maturation with royal jelly increases
embryo development and reduces apoptosis in goats. Animal Repro
duction, 15(2): 124.
Vidal, A.H. et al., 2013. Soybean lecithin-based extender as an alternative
for goat sperm cryopreservation. Small ruminant research, 109(1):
-51.
Yodmingkwan, P., Guntaprom, S., Jaksamrit, J. and Lertchunhakiat, K.,
Effects of extenders on fresh and freezing semen of boer goat.
Agriculture and Agricultural Science Procedia, 11: 125-130.
Yotov, S.A., Velislavova, D.V. and Dimova, L.R., 2016. Pregnancy rate
in Bulgarian White milk goats with natural and synchronized estrus
after artificial insemination by frozen semen during breeding season.
Asian Pacific Journal of Reproduction, 5(2): 144-147.
Youngquist, R.S. and Threlfall, W.R., 2006. Current Therapy in Large An
imal Theriogenology-E-Book. Elsevier Health Sciences.
Zahmatkesh, E., Najafi, G. and Nejati, V., 2015. Protective effect of royal
jelly on in vitro fertilization (IVF) in male mice treated with oxy
metholone. Cell Journal (Yakhteh), 17(3): 569.
Zahmatkesh, E., Najafi, G., Nejati, V. and Heidari, R., 2014. Protective ef
fect of royal jelly on the sperm parameters and testosterone level and
lipid peroxidation in adult mice treated with oxymetholone. Avicenna
journal of phytomedicine, 4(1): 43
