Investigation of Genetic Polymorphisms of CSN1S1 and BLG Genes in Norduz sheep by PCR-RFLP method

PDF
Published: Jan 18, 2024
Updated: 2024-01-18
DOI: https://doi.org/10.12681/jhvms.31872
Keywords:
Alpha-S1 Casein β-Lactoglobulin PCR-RFLP Norduz Sheep
B Çak
Department of Zootechny, Faculty of Veterinary Medicine, University of Van YuzuncuYil, Van, Turkey
O Yılmaz
Department of Zootechny, Faculty of Veterinary Medicine, University of Van YuzuncuYil, Van, Turkey
https://orcid.org/0000-0002-6261-5196
AF Demirel
Department of Zootechny, Faculty of Veterinary Medicine, University of Van YuzuncuYil, Van, Turkey
https://orcid.org/0000-0002-7905-5850
Abstract

This study was undertaken to examine genetic polymorphisms of Alpha-S1 casein (CSN1S1) and β-Lactoglobulin (BLG) genes in Norduz sheep by PCR-RFLP method. In the study, 10 ml blood samples were taken from 102 heads of Norduz sheep into tubes with EDTA. DNA isolation from blood samples was done with ready-made commercial kits. The target DNA region of alpha-s1 casein and beta-lactoglobulin genes was amplified by PCR technique. The obtained PCR patterns were digested with MboII and RsaI restriction enzymes to determine the genotypes of Alpha-S1 casein and β-lactoglobulin genes, respectively. The cut fragments were run on 2% agarose gel electrophoresis and genotypes were determined under UV light. Statistical analysis, after calculating allele and genotype frequencies by direct gene counting method, the distribution of observed and expected genotypic frequencies was determined according to the Hardy-Weinberg Equilibrium, and whether it was compatible with the χ2 test. β-Lactoglobulin AA, AB and BB genotype frequencies were found to be 17.6%, 69.6 and 12.7%, respectively. It was determined that the β-Lactoglobulin gene was not in Hardy-Weinberg Equilibrium. AlphaS1-Casein AA, AC and CC genotype frequencies were determined as 0.0, 2.9 and 97.1%, respectively. The Alpha-S1 casein gene was determined to be in Hardy-Weinberg equilibrium. It was determined that Norduz sheep showed polymorphism in terms of Alpha-S1 Casein and β-Lactoglobulin genes. In conclusion, it was determined that the A allele of the β-Lactoglobulin gene and the AB genotype were more common, the C allele and the CC genotype of the Alpha-S1 casein gene were more common, and the AA genotype was absent in Norduz sheep. It was concluded that it would be useful to investigate the economic effects of genetic polymorphisms of Alpha-S1 casein and β-Lactoglobulin genes on various yield traits in larger Norduz sheep populations.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Aggeler J, Park CS, Bissell MJ (1988). Regulation of milk protein and basement membrane gene expression: the influence of the extracellu lar matrix. J Dairy Sci71(10):2830-2842.
Anton I, Zsolnai A, Fesus L, Kukovics S, Molnar A (1999). Survey of β-lactoglobulin and alfa S1-Casein polymorphism in Hungarian dairy sheep breeds and crosses on DNA level. Arch Anim Breed42:387-392.
Barillet F, Arranz JJ, CartaA (2005). Mapping quantitative trait loci for milk production and genetic polymorphisms of milk proteins in dairy sheep. Genet Sel Evol37(Suppl. 1):109-123.
Beuzen ND, Stear MJ, Chang KC (2000). Molecular markers and their use in animal breeding. The Veterinary Journal, 160(1), 42-52.
Chaudhary R, Kumar GM (2019). Restriction fragment length polymor phism. Encyclopedia of Animal Cognition and Behavior; Vonk, J., Shackelford, TK, Eds. Çelik Ş, Özdemir S (2006). β-Lactoglobulin Variants in Awassi and Mork araman Sheep and their Association with the Composition and Rennet Clotting Time of the Milk. Turkish J Vet Anim Sci 30(6):539-544.
Corral JM, Padilla JA, Izquierdo M (2010). Associations between milk protein genetic polymorphisms and milk production traits in Merino sheep breed. Livest Sci 129(1-3):73-79.
Elmacı C, Öner Y, Balcıoğlu MS (2006). Genetic polymorphism of β-lac to-globulin gene in Native Turkish sheep breeds. Biochem Genet 44:379-384.
Erhardt G (1989). Evidence for a third allele at the β-lactoglobulin (BLG) locus of sheep and its occurrence in different breeds. Anim Genet 20(3):197-204.
Ezelhan Ş, Keleş A, İşler S (2021). Macrofungal biodiversity of Gürpınar (Van) district. Ant J Bot5(1):23-28.
Feligini M, Parma P, Aleandri R, Greppi GF, Enne E (1998). PCR-RFLP test for direct determination of β-lactoglobulin genotype in sheep. Anim Genet29(6):473-474.
Frajman P, Dovc P (2004). Milk production in the post-genomic era. Acta Agric Slov84(2):109-119.
Gencheva D, Veleva P, Naidenova N, Pamukova D (2020). Genetic poly morphism of alpha S1-casein in Bulgarian sheep breeds and its effect on milk composition. Turkish J Vet Anim Sci44(4):860-870.
Giambra IJ, Brandt H, Erhardt G (2014). Milk protein variants are highly associated with milk performance traits in East Friesian Dairy and Lacaune sheep. Small Rumin Res 121(2-3):382-394.
Kevorkian S, Manea M, Gavrila M, Rebedea M, Georgescu S,Costache M (2009). Sequencing of exon three of αs1-casein sheep and goat gene. Arch. zootech 12(3):87-92.
Mercier JC, Gaye P, Soulier S, Hue-Delahaie D, Vilotte JL (1985). Con struction and identification of recombinant plasmids carrying cDNAs coding for ovine αS1-,αS2-, ß,κ-casein and ß-lactoglobulin. Nucleotide sequence of αS1-casein cDNA. Biochimie 67(9):959- 971.
Mohammadi A, Nassiry MR, Elyasi G, Shodja J (2006). Genetic polymor phism of β-lactoglobulin in certain Iranian and Russian sheep breeds. Iran J Biotechnol4(4):265-268.
Morammazi S, Masoudi AA, Torshizi RV, Pakdel A (2016). Differential expression of the alpha s1 casein and beta-lactoglobulin genes in dif ferent physiological stages of the Adani goats mammary glands. Iran J Biotechnol14(4):278-285.
Pilla F, Bevilacquea C, Leroux C, Fraghi A, Martin P (1998). Genotyping of α-s1 casein in sheep. Anim Genet 29(6):472-473.
Ramos AM, Matos CAP, Russo-Almeida PA, Bettencourt CMV, Matos J, Martins A, Pinheiro C, Rangel-Figueiredo T (2009). Candidate genes for milk production traits in Portuguese dairy sheep. Small Rumin Res 82:117-121.
Recio I, Fernandez-Fournier A, Martin-Alvarez PJ, Ramos M (1997). β-Lactoglobulin polymorphism in ovine breeds: Influence on cheese-making properties and milk composition. Lait 77(2):259-265.
Rout PK, Kumar A, Mandal A, Laloe D, Singh SK, Roy R (2010). Char acterization of casein gene complex and genetic diversity analysis in Indian goats. Anim Biotechnol21(2):122-134.
Rustempasic A, Dokso A, Zecevic E, Hodzic A, Bandzo K, Miskoska-Mi levska E, Popovski Z, Brka M (2013). Polymorphism of CSN1S1 gene in Pramenka breed sheep in Bosnia and Herzegovina. In: Pro ceedings of the 24th International Scientific-Expert-Conference of Agriculture and Food Industry, Sarajevo, Bosnia and Herzegovina, Faculty of Agriculture and Food Sciences, University of Sarajevo: pp 91-94.
Sahin E, Karslı T, Balcıoğlu MS (2011). Beta-Lactoglobulin gene types in Turkish fat-tailed sheep breeds. Kafkas Univ Vet Fak Derg 17(6):1031-1033.
Sanchez A, Ilahi H, Manfredi E, Serradilla JM (2005). Potential benefit from using the alpha(s1)-casein genotype information in a selection scheme for dairy goats. J Anim Breed Genet 122(1):21-29.
Schlee P, Rottmann O (1992). Sheep β-lactoglobulin: determination of alleles A and B by PCR and RFLP analysis using plucked hair as a source of DNA. In: Genetic Conservation of Domestic Livestock.Vol. 2, CAB International,Wallingford,UK:pp. 243-246.
Schmoll F, Herget I, Hatzipanagiotou A, Tholen E, Wimmers K, Brem G, Schellanger K (1999). Association of β lactoglobulin variants with milk production, milk composition and reproductive performance in milk sheep. Wien TierarztlMonatsschr86:57-60.
Tagem (2009). Türkiye çiftlik hayvanları genetik kaynakları kataloğu. Tarımve Köyişleri Bakanlığı, Tarımsal Araştırmalar Genel Müdürlüğü, Ankara.
Turkstat (2021). Turkish Statistical Institute Statistics. [online]. Website https://data.tuik.gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatis tikleri-Haziran-202137208&dil=1) [Accessed 11 May, 2022].
Turkvet (2022). Tarımve Orman Bakanlığı Hayvan Kayıt Sistemi. [on line]. Website http://hbs.tarim.gov.tr/ [Accessed 11 May, 2022]