The effect of subclinical mastitis on milk transforming growth factor-beta level in Anatolian water buffaloes

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Published: Apr 19, 2025
DOI: https://doi.org/10.12681/jhvms.38343
Keywords:
buffalo milk subclinical mastitis transforming growth factor-beta
H Gürler
Samsun Ondokuz Mayıs University Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Samsun, Türkiye
GF Yarım
Samsun Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Biochemistry, Samsun, Türkiye
M Yarım
Samsun Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Pathology, Samsun, Türkiye
https://orcid.org/0000-0002-0636-4214
A Gökçeoğlu
Adana Çukurova University, Faculty of Veterinary Medicine, Department of Biochemistry, Adana, Türkiye
F Ümit
Samsun Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Biochemistry, Samsun, Türkiye
Abstract

This study aims to evaluate the relationship between transforming growth factor beta (TGF-β) and somatic cell count (SCC) in Anatolian buffalo milk.  The study material comprised 80 milk samples obtained from the udder quarter of 40 healthy and 40 subclinical mastitic nonpregnant lactating Anatolian buffaloes. The California mastitis test and somatic cell count were performed on milk samples milked from four mammary lobes of buffaloes to diagnose subclinical mastitis. The milk TGF-β level was measured by bovine-specific enzyme-linked immunosorbent assay kit. The milk TGF-β levels in subclinical mastitic and control groups were 11.7±2.6 (8.0 to 18.3) ng/ml and 4.8±1.3 (2.4 to 7.0) ng/ml. The milk TGF-β level of subclinical mastitic buffaloes was significantly higher than healthy ones (P<0.01). A significant positive correlation was determined between the milk TGF-β level and SCC in subclinical mastitic buffaloes (r2 = .936, P < 0.001). This is the first study to determine the relationship between TGF-β concentrations and SCC in milk obtained from mammary quarters with subclinical mastitis in Anatolian water buffaloes. This study has the potential to contribute to studies on cytokine changes in milk in subclinical mastitis of buffaloes.

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