test In situ and In Vitro Rumen Protein Degradability of Soybean Products: Relationship Estimation|Journal of the Hellenic Veterinary Medical Society

In situ and In Vitro Rumen Protein Degradability of Soybean Products: Relationship Estimation

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Published: Nov 5, 2025
DOI: https://doi.org/10.12681/jhvms.38480
Keywords:
in situ in vitro enzymatic method Streptomyces griseus
A Erol Tunç
International Center for Livestock Research and Training, 06852 Ankara, Türkiye
Y Cufadar
Department of Animal Science, Faculty of Agriculture, Selçuk University, 42130 Konya, Türkiye
SY Fırıncıoğlu
Department of Animal Production and Technologies, Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, 51240 Niğde, Türkiye
Abstract

Bu bilgiler, Tam Yağlı Soya Fasulyesi (YFS) ve Soya Fasulyesi Küspesi'nin (KM) proteinin parçalanabilirliğini in situ (naylon torba) ve in vitro enzimatik olarak enjekte edildiğini tahmin etmek ve in vitro değerlerden in situ parçalanabilirliğini tahmin etmek için bir denklemin çoğaltılmasıdır. Çalışmada enzimatik teknik olarak; pH 8'de borat-fosfat tamponunda Streptomyces griseus'tan ekstrakte edilen saflaştırılmış bir proteaz ile 1 saat sonra (INV1) ve 24 saat sonra (INV24) hidroliz, in vitro yöntem olarak kullanılmıştır. In situ etkili protein parçalanabilirliği (INSE) ile 1 (INV1) ve 24 (INV24) saatlik inkübasyonlardan sonraki in vitro parçalanabilirlik arasındaki ilişki belirlendi. Yerinde protein parçalanabilirliği 3 Holstein ineğin rumeninde 0, 2, 4, 8, 16, 24, 48 ve 72 saatlik inkübasyonlarda ölçülmüştür. Çalışmada FFS'nin INSE, INV1 ve INV24 değerleri sırasıyla 0.81, 0.60 ve 0.76 olarak belirlendi. Söz konusu etki SM'de sırasıyla 0.59, 0.19 ve 0.52 olarak belirlendi. İn situ ve in vitro protein parçalanabilirliği değerleri arasında farklılıklar olmasına rağmen, FFS ve SM'nin in situ ve in vitro protein parçalanabilirliği arasındaki dağılım katsayıları yüksek haddeydi ve in vitro'dan in situ'nun tahminine yönelik regresyon denklemleri anlamlıydı. FFS ve SM'den bir grup oluşturu tüm karakteristik denklemler önemli özellikler ve dağılım katları değişiklikleri. İn vitro enzimatik protein parçalanabilirliği (INV1 ve INV24), FFS ve SM'nin in situ etkili protein parçalanabilirliğinin tahmininde kullanılabilir. FFS ve SM'den hesaplama grup denklemlerinde INSE'nin daha az emek, daha az maliyet ve daha kısa sürede tahmin edilmesi için INV1'in uygun şekilde doldurulabileceği varılmıştır.

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References
AFRC, 1987, Characterisation of feedstuffs: Nitrojen, Rep. No: 2, Technical
Committe on Responses to Nutrients Nutr. Abstr. Rev. Ser.
B.. Livest. Feeds Feeding (57), 713-736.
AOAC, 1995, Offical methods of analysis 16th Edition (Arlington.
Virginia. USA).
Assoumani, M., Vedeau, F., Jacquot, L. ve Sniffen, C., 1992, Refinement
of an enzymatic method for estimating the theoretical degradability
of proteins in feedstuffs for ruminants, Animal Feed Science and
Technology, 39 (3-4), 357-368.
Aufrère, J., Graviou, D., Demarquilly, C., Verite, R., Michalet-Doreau,
B. ve Chapoutot, P., 1991, Predicting in situ degradability of feed
proteins in the rumen by two laboratory methods (solubility and
enzymatic degradation), Animal Feed Science and Technology, 33
(1-2), 97-116.
Aufrere, J. ve Cartailler, D., 1988, Mise au point d’une méthode de
laboratoire de prévision de la dégradabilité des protéines alimentaires
des aliments concentrés dans le rumen, Ann. Zootech, 37
(4), 255-270.
Broderick, G. A., Wallace, R., Ørskov, E. ve Hansen, L., 1988, Comparison
of estimates of ruminal protein degradation by in vitro and
in situ methods, Journal of Animal Science, 66 (7), 1739-1745.
Calsamiglia, S. ve Stern, M. D., 1995, A three-step in vitro procedure
for estimating intestinal digestion of protein in ruminants, Journal
of Animal Science, 73 (5), 1459-1465.
Canbolat, O., Kamalak, A., Efe, E., Sahin, M. ve Ozkan, C., 2005, Effect
of heat treatment on in situ rumen degradability and in vitro gas
production of full-fat soyabeans and soyabean meal, South African
Journal of Animal Science, 35 (3), 186-194.
Chaudhry, A., 2005, Comparing two commercial enzymes to estimate
in vitro proteolysis of purified or semi-purified proteins, Journal
of animal physiology and animal nutrition, 89 (11-12), 403-412.
Chaudhry, A. S., 2007, Enzymic and in sacco methods to estimate rumen
degradation of food protein in cattle, Journal of the Science of Food
and Agriculture, 87 (14), 2617-2624.
Chamberlain, A. T., and Wilkinson, J. M., 1996. Feeding the dairy
cow.,(Chalcombe Publications: Lincoln, UK).
Cherney, D., Patterson, J. and Lemenager, R., 1990, Influence of in Situ
Bag Rinsing Technique on Determination of Dry Matter Disappearance1,
, Journal of Dairy Science, 73 (2), 391-397.
Cömert, M. ve Șayan, Y., 2000, Research on rumen degradation of
protein of feeds used in ruminant nutrition, Ege Üniversitesi Ziraat
Fakültesi Dergisi, 37 (1), 145-152.
Deniz, S., Karsli, M. A., Nursoy, H. ve Kutlu, M. S., 2004, Ruminantların
Beslenmesinde Yaygın Olarak Kullanılan Proteince Zengin Bazı
Yem Hammaddelerinin Protein Parçgalanabilirlik Özelliklerinin
In Sacco Yöntemle Belirlenmesi, Turkish Journal of Veterinary &
Animal Sciences, 28 (6).
González, J., Andrés, S., Rodríguez, C. A. ve Alvir, M. R., 2002, In
situ evaluation of the protein value of soybean meal and processed
full fat soybeans for ruminants, Animal Research, 51 (6), 455-464.
Griffiths, J. B., 2004, The effect of extrusion on the degradability parameters
of various vegetable protein sources, Stellenbosch University.
Kirkpatrick, B. ve Kennelly, J., 1987, In situ degradability of protein
and dry matter from single protein sources and from a total diet,
Journal of Animal Science, 65 (2), 567-576.
Maxin, G., Ouellet, D. ve Lapierre, H., 2013, Ruminal degradability
of dry matter, crude protein, and amino acids in soybean meal,
canola meal, corn, and wheat dried distillers grains, Journal of
Dairy Science, 96 (8), 5151-5160.
Madsen, J. ve Hvelplund, T., 1994, Prediction of in situ protein degradability
in the rumen. Results of a European ringtest, Livestock
production science, 39 (2), 201-212.
McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA. 2011. Animal
Nutrition (Seventh Edition). Pearson, Harlow, Essex, UK: Pearson
Education Limited. ISBN-13: 978-1408204238.
Michalet-Doreau, B. and Cerneau, P., 1991, Influence of foodstuff particle
size on in situ degradation of nitrogen in the rumen, Animal
Feed Science and Technology, 35 (1-2), 69-81.
Michalet-Doreau, B., Vérité, R. ve Chapoutot, P., 1987, Méthodologie
de mesure de la dégradabilité in sacco de l’azote des aliments dans
le rumen, Bull. Tech. CRZV Theix, 69, 5-7.
Mohamed, R. ve Chaudhry, A. S., 2008, Methods to study degradation
of ruminant feeds, Nutrition research reviews, 21 (01), 68-81.
Nobar, R. S., Chamani, M., Sadeghi, A. ve Aghazadeh, A., 2009, Determination
of degradability of treated soybean meal and its proteins
fractions, African Journal of Biotechnology, 8 (1).
Nowak, W., Michalak, S. ve Wylegala, S., 2005, In situ evaluation
of ruminal degradability and intestinal digestibility of extruded
soybeans, Czech J. Anim. Sci, 50, 281-287.
NRC, 2001, Nutrient requirements of dairy cattle, National Academy
Press, Washington, DC.
Orskov, E., 1982, Protein nutrition in Ruminants. Academic press Inc,
London.
Orskov, E., 1988, Feed Science, The Rowett Research Institute. Bucksburn.
Aberdeen. AB2. 9SB Scotland.
Orskov, E. ve McDonald, I., 1979, The estimation of protein degradability
in the rumen from incubation measurements weighted
according to rate of passage, The Journal of Agricultural Science,
(02), 499-503.
Krishnamoorthy, U., Sniffen, C., Stern, M. ve Van Soest, P., 1983,
Evaluation of a mathematical model of rumen digestion and an in
vitro simulation of rumen proteolysis to estimate the rumen-undegraded
nitrogen content of feedstuffs, British Journal of Nutrition,
(03), 555-568.
Poos-Floyd, M., Klopfenstein, T. ve Britton, R., 1985, Evaluation of
Laboratory Techniques for Predicting Ruminal Protein Degradation1,
Journal of Dairy Science, 68 (4), 829-839.
Roe, M. B., Chase, L. ve Sniffen, C., 1991, Comparison of in vitro
techniques to the in situ technique for estimation of ruminal degradation
of protein, Journal of Dairy Science, 74 (5), 1632-1640.
Sacakli, P., Tuncer, S. D., Koksal, B. H., Selcuk, Z. ve Genc, B., 2011,
The effect of xylose treatment on dry matter and crude proteın
degradabılıty characterıstıcs of soybean meal, full fat soybean and
soybean seed ın sheep, Veterinarija ir Zootechnika, 56 (78), 87-92.
Sarıçiçek, B. Z., 1999, Etil Alkol ile Muamelenin Bazı Protein Kaynaklarının
İn Situ Rumen Parçalanabilirliği Üzerine Etkisi, Tr. J.
of Veterinary and Animal Sicences, 23, 515-522.
Susmel, P., Stefanon, B., Mills, C. ve Colitti, M., 1989, The evaluation
of PDI concentrations in some ruminant feedstuffs: a comparison of
in situ and in vitro protein degradability, Ann. Zootech, 38, 269-283.
Van Soest, P. v., Robertson, J. ve Lewis, B., 1991, Methods for dietary
fiber, neutral detergent fiber, and nonstarch polysaccharides
in relation to animal nutrition, Journal of Dairy Science, 74 (10),
-3597.
Vérité, R., Michalet-Doreau, B., Chapoutot, P., Peyraud, J. ve Poncet, C.,
, Révision du système des protéines digestibles dans l’intestin
(PDI), Bull. Tech. CRZV Theix INRA, 70, 19-34.
Woods, V., O’Mara, F. ve Moloney, A., 2003, The nutritive value of
concentrate feedstuffs for ruminant animals: Part I: In situ ruminal
degradability of dry matter and organic matter, Animal Feed Science
and Technology, 110 (1), 111-130.
Yalcin, S., Şehu, A. ve Önol, A., 1998, Straw degradability as a predictor
of intake and growth rate in sheep, Animal Science, 67 (03),
-490.
Yörük, M. A., Gül, M., Aksu, T. ve Bolat, D., 2003, Formaldehitle Muamele
Edilmiş Soya Küspesinin Rumende Parçalanma Özellikleri,
Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 14 (2), 1-5.
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