Detection of miRNA-15a, miRNA-29b, miRNA-34a and miRNA-223 in Cow, Sheep, Goat, Buffalo, and Donkey Milk and Investigation of Their Relationship with Milk Composition

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Published: Jul 5, 2025
DOI: https://doi.org/10.12681/jhvms.39515
Keywords:
Donkey milk milk composition miRNA miRNA-15a miRNA-34a
ahmet çelik
a:1:{s:5:"en_US";s:19:"dicle üniversitesi";}
AYDIN VURAL
dicle üniversitesi
İBRAHİM HALİL YILDIRIM
Abstract

The objective of this study was to ascertain the expression levels of miRNA-15a, miRNA-29b,
miRNA-34a, and miRNA-223 in milk from cows, buffaloes, sheep, goats, and donkeys and to examine their
relationship with milk composition. The miRNA expression levels were quantified by RT-qPCR, and their correlation
with the physicochemical data of the milk was examined. The expression of miRNAs was observed in
all milk samples and there was a correlation between miRNA expression and milk fat, protein, dry matter and
pH. A positive correlation was observed between fat and miRNA-34a in cows’milk, a positive correlation between
fat and miRNA-29b in donkey milk (P<0.05), and a negative and significant correlation between fat and
miRNA-29b in sheep milk (P<0.05). A positive and significant correlation was identified between dry matter
and miRNA-34a in sheep milk (P<0.05), whereas a negative and significant correlation was observed between
dry matter and miRNA-223 in goat milk (P<0.05). Additionally, a negative and significant correlation was found
between dry matter and miRNA-29b in sheeps’milk (P<0.05). A positive and significant relation ship was identified
between miRNA-34a expression and pH in sheep milk (P<0.05). It can be concluded that miRNAs play an
important role in milk composition, hygiene, health and technology. Further studies are recommended to gain a
deeper understanding of these aspects.

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