test Pathological characterization of lung lesions and molecular detection of Mycoplasma spp. in slaughtered goats|Journal of the Hellenic Veterinary Medical Society

Pathological characterization of lung lesions and molecular detection of Mycoplasma spp. in slaughtered goats

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Published: Nov 10, 2025
DOI: https://doi.org/10.12681/jhvms.40322
Keywords:
goat lung Histopathology PCR sequencing
I Vishnubashini
Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India
https://orcid.org/0009-0004-8763-6833
R Priyadharshini
Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India
Praveen K
PG Scholar
Poobitha S
Assistant Professor
https://orcid.org/0000-0003-2860-1652
S Uma
Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India
MV Srinivas
Department of Centre for Translational Research, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India
AW Lakkawar
Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India
K Raja
Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India
https://orcid.org/0000-0001-8843-1478
Abstract

   Mycoplasmosis is a chronic respiratory disease of goat causing huge economic loss to the farmers. A slaughter house based study was carried out from December 2023 to March2024 to characterize the gross, histopathological lesions in lungs and also to confirm the role of Mycoplasma spp. by PCR in slaughtered goats in Puducherry, India. Out of 350 carcasses examined, pneumonic lesions were recorded in 104 cases accounting for 29.7 %. All the affected animals in the present study were above 1 year of age. Sex wise lesions were recorded more in males (60.5%, 63/104) than females (39.4% 41/104). Breed wise, the occurrence of lung lesions were: salem black (28.8%, 30/104), kodiaadu (26.9%, 28/104) and non-descript (44.2%, 46/104). Gross examination revealed emphysematous, edematous and grey to red areas of consolidation with exudation from the cut surface. Representative tissue samples were collected and fixed in 10% formalin and processed by routine paraffin embedding techniques. Histopathological examination of affected lung tissue showed extensive area of haemorrhage, pleural thickening, bronchus associated lymphoid tissue [BALT] hyperplasia, septal thickening, epithelial hyperplasia and mononuclear infiltration. From histopathologically confirmed pneumonic cases, 6 samples were subjected to PCR targeting the 16srRNA specific for Mycoplasma spp. Out of 6 cases, four samples yielded an expected amplicon size of 450 bp (66.66%) confirming the involvement of Mycoplasma spp.


 

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References
Adehan RK, Ajuwape ATP, Adetosoye AI, Alaka OO (2006) Characterization
of Mycoplasmas isolated from pneumonic lungs of sheep
and goats. Small Rumin Res 63(1-2): 44-49.
Alberti A, Addis MF, Chessa B, Cubeddu T, Profiti M, Rosati S, Ruiu
A, PittauM (2006) Molecular and antigenic characterization of a
Mycoplasma bovis strain causing an outbreak of infectious keratoconjunctivitis.
J Vet Diagn Invest 18(1): 41-51.
Awan MA, Abbas F, Yasinzai M, Tariq MM, Bajwa MM, Attique MA,
Shafee M (2012) Prevalence of Mycoplasma species by polymerase
chain reaction (PCR) directly from the nasal swab samples of goats.
Pak J Lif Soc Sci 10(1): 5-12.
Chazel M, Tardy F, Le Grand D, Calavas D, Poumarat F (2010) Mycoplasmoses
of ruminants in France: Recent data from the national
surveillance network. BMC Vet Res 6(32): 1-8.
Citti C, Blanchard A (2013). Mycoplasmas and their host: emerging and
re-emerging minimal pathogens. Trends Microbiol 21(4):196–203.
DhaygudeV, Kamdi B, Barate A, Sukare J, Sabharwal D, Tumla U
and Mote C(2023). Pathological and molecular diagnosis of contagious
caprine pleuropneumonia (CCPP) in naturally infected goats
(Capra hircus) from Maharashtra, India. Emerging Animal Species
:100024
El-Deeb W, Almujalli AA, Eljalii I, Elmoslemany A, Fayez M (2017)
Contagious caprine pleuropneumonia: The first isolation and molecular
characterization of Mycoplasma capricolum subsp. capripneumoniae
in the Kingdom of Saudi Arabia. Acta Trop168: 74-79.
Halium MMA, Salib FA, Marouf SA, Massieh ESA (2019) Isolation
and molecular characterization of Mycoplasma spp. in sheep and
goats in Egypt. Vet World 12(5): 664-670.
Minion FC (2002) Molecular pathogenesis of mycoplasma animal respiratory
pathogens. Front Biosci 7(1): 1410-1422.
Nicholas R, Ayling R, McAuliffe L (2008) ‘Isolation and growth of
Mycoplasmas from ruminants’, in Nicholas, R., Ayling, R., and
McAuliffe, L. (eds), Mycoplasma Diseases of Ruminants: International
CABI, Wallingford, UK: CABI Publishing, 3–14.
Noll LW, Highland MA, Hamill VA, Tsui WNT, Porter EP, Lu N, Sebhatu
T, Brown S, Herndon DR, Grossman PC, Bai J (2022). Development
of a real-time PCR assay for detection and differentiation of
Mycoplasma ovipneumoniae and a novel respiratory-associated
Mycoplasma species in domestic sheep and goats. Transbound
Emerg Dis 69(5): e1460–e1468.
Pavone S, Crotti S, D’Avino N, Gobbi P, Scoccia E, Pesca C, Gobbi M,
Cambiotti V, Lepri E, Cruciani D (2023). The role of Mycoplasma
ovipneumoniae and Mycoplasma arginini in the respiratory mycoplasmosis
of sheep and goats in Italy: Correlation of molecular data
with histopathological features. Res Vet Sci 163: Article 104983..
Razin S, Yogev D, Naot Y (1998). Molecular biology and pathogenicity
of mycoplasmas. Microbiol Mol Biol 62(4), 1094-1156.
Rehman FU, KhanFA, Saeed M, Ali M, KhanH, Ahmad F and Ullah Q
(2022). Clinicopathological and Sero-Molecular Detection of Mycoplasma
capricolum subsp. capripneumoniae in Goats in Southern
Areas of Pakistan. Vet medint, 9508810
Sheikh FD, Wani SA, Fatima K, Masoodi TH (2016) Contagious caprine
pleuropneumonia: A major threat to Pashmina farming in Ladakh.
High Mountain Arid Agricultural Research Institute Technical Bulletin
Series 16(4): 1-8.
Valsala R, Rana R, Remesh AT, Singh VP (2017) Mycoplasma arginini:
High frequency involvement in goat pneumonia. Turk J Vet Anim
Sci 41(3): 393-399.
Yatoo MI, Kanwar MS (2016) Management of common diseases of livestock
in Changthang. Krishi Vigyan Kendra Bulletin Series (3): 1-16.
Yatoo MI, Parray OR, Mir MS, Qureshi SQ, Kashoo ZA, Nadeem MN,
Rana RR (2018) Mycoplasmosis in small ruminants in India: A
review. J Exp Biol Agric Sci 6(2): 264-281.