High proliferation of Pseudo-nitzschia cf. arenysensis in the Adriatic Sea: ecological and morphological characterisation


Veröffentlicht: Δεκ 1, 2020
JASNA ARAPOV
https://orcid.org/0000-0001-7568-1929
MIA BUŽANČIĆ
https://orcid.org/0000-0003-3848-9561
ANTONELLA PENNA
SILVIA CASABIANCA
SAMUELA CAPELLACCI
FRANCESCA ANDREONI
SANDA SKEJIĆ
ANA BAKRAČ
MAJA STRAKA
JELENA JELENA MANDIĆ
ŽIVANA NINČEVIĆ GLADANA
Abstract

The aim of the present study isto characterise the diatom Pseudo-nitzschia community during a bloom period together with environmental conditions. High proliferation of Pseudo-nitzschia spp. was observed in September 2017 at the shellfish breeding area in the Krka River estuary (Central eastern Adriatic Sea). The peak of abundance (1.8 x 106 cells L-1) was recorded at 7 m depth, and the increased abundance persisted for four weeks.Morphological analyses of field samples based on scanning electron microscopy (SEM) revealed that Pseudo-nitzschia cf. arenysensis was prevailing (94%) in the Pseudo-nitzschia assemblage. Several strains were successfully isolated from net samples in order to better define morphological features and phylogenetic characterisation. The isolated Pseudo-nitzschia strains corresponded morphologically to the P. cf. arenysensis from the field samples, based on our SEM observations. Phylogenetic analysis demonstrated that the Croatian strains grouped with P. arenysensis using the ITS and LSU rDNA sequences. Spearman rank correlation showed that salinity was an important environmental factor affecting the vertical distribution of Pseudo-nitzschia spp. in this highly variable area. Availability of increased concentration of orthophosphates and ammonium and low Si: TIN ratio may have promoted the bloom of P. cf. arenysensis in the estuary.

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MIA BUŽANČIĆ, Institute of Oceanography and Fisheries, Croatia
Laboratory of Plankton and Shellfish Toxicity
ANTONELLA PENNA, University of Urbino, Department of Biomolecular Sciences, Italy
Department of Biomolecular Sciences
SILVIA CASABIANCA, University of Urbino, Department of Biomolecular Sciences, Italy
Department of Biomolecular Sciences
SAMUELA CAPELLACCI, University of Urbino, Department of Biomolecular Sciences, Italy
Department of Biomolecular Sciences
FRANCESCA ANDREONI, University of Urbino, Department of Biomolecular Sciences, Italy
Department of Biomolecular Sciences
SANDA SKEJIĆ, Institute of Oceanography and Fisheries, Croatia
Laboratory of Plankton and Shellfish Toxicity
ANA BAKRAČ, Institute of Oceanography and Fisheries, Croatia
Laboratory of Plankton and Shellfish Toxicity
MAJA STRAKA, Institute of Oceanography and Fisheries, Croatia
Laboratory of Plankton and Shellfish Toxicity
JELENA JELENA MANDIĆ, Institute of Oceanography and Fisheries, Croatia

Laboratory of Chemical Oceanography and Sedimentology of the Sea

 

 

ŽIVANA NINČEVIĆ GLADANA, Institute of Oceanography and Fisheries, Croatia
Laboratory of Plankton and Shellfish Toxicity
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