Effect of Nutrient, Light Intensity and Temperature on the Growth Rates and Metabolism of a Stress-Resistant Bacillariophyta – Entomoneis sp. - in Izmir Bay (Aegean Sea)


SEZGI ADALIOĞLU
https://orcid.org/0000-0001-5095-1400
GÜLIZAR ÇALIŞKAN
https://orcid.org/0000-0001-6221-9495
Résumé

A unicellular marine microalga, Entomoneis sp. was isolated and studied as had become the dominant species according to other bacillariophyta species in different environmental fluctuations in Izmir Bay. Because of our effort to better understand the dynamics of this microalga that facilitates unprecedented domination, we conducted on a monoculture isolation study.In this study, experiments were planned with the annual range of the Izmir Bay temperature, and the demonstrated behavior of the species in light and nutrient conditions. The stock culture medium was illuminated by approximately 50μmol photons m-2s-1 of illumination with 14/24 daylight. The temperature of the climate chamber was set on the summer (T1 (21±1oC)), spring (T2 (17±1oC)) and winter (T3 (13±1oC)) of Izmir Bay. Experiments were also applied with four different light intensities (L1 (50 μmol photons m-2s-1), L2 (25 μmol photons m-2s-1), L3 (5 μmol photons m-2s-1) and L4 (dark)). In this context, nutrient measurements were made on samples of the exponential, stationary and death phase of the culture and nutrient analyses were carried out. The results, which were designed according to ceteris paribus assumptions, were adapted to Michaelis-Menten kinetics. Consequently, considering the lifetime of the diatom at different temperature conditions, T3 was determined as an optimum temperature. Maximum growth rate and process time were observed at this temperature. This is the evidence why these diatoms are available in the winter. Once the light intensity was increased, the growth rate was increased at the T1 and T2 temperatures. However, T3 had a high growth rate in the nearly L1 light intensity. Considering the consumption and transformation of different nutrient conditions, different results for both types of microalgae were obtained.

 

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Références
Abdelaziz, A.E.M., Leite, G.B., Belhaj, M.A., Hallenbeck, P.C., 2014. Screening microalgae native to Quebec for wastewater treatment and biodiesel production. Bioresource Technology, 157, 140-148.
Adalioǧlu, S., Buyukisik, B., Yasar, D., 2013. Microplankton growth in response to nutrient enrichments in Gerence Bay, Izmir, Western Turkey. Indian Journal of Marine Sciences, 42, 859-867.
Aysel, V., Aysel, F., 2002. Barutçu Gölü’ ün (Selçuk, İzmir, Türkiye) mikro ve makro algleri, Ege Üniversitesi Su Ürünleri Dergisi, 19, 1-11.
Breuer, G., Lamers, P.P., Martens, D.E., Draaisma, R.B., Wijffels, R.H., 2013. Effect of light intensity, pH, and temperature on triacylglycerol (TAG) accumulation induced by nitrogen starvation in Scenedesmus obliquus. Bioresource Technology, 143, 1-9.
Brindley, C., Acian, F.G., Fernandez-Sevilla, J.M., 2010. The oxygen evolution methodology affects photosynthetic rate measurements of microalgae in well-defined light regimes. Biotechnology and Bioengineering, 106, 228-237.
Cabello, J., Toledo-Cervantes, A., Sanchez, L., Revah, S., Morales,M., 2015. Effect of the temperature, pH and irradiance on the photosynthetic activity by Scenedesmus obtusiusculus under nitrogen replete and deplete conditions. Bioresource Technology, 181, 128-135.
Clavero, E., Grimalp, J., Marine, M.H., 1999. Entomoneis vertebralis sp. nov. (Bacillariophyceae); a new species from hypersaline environments. Cryptogamie Algologie, 20, 223-234.
Collos, Yves; Slawyk, G., 1980. Nitrogen uptake and assimilation by marine phytoplankton. Primary Productivity in the Sea, 19, 195-211.
Czarnecki, D.B., Reinke, D.C., 1982. Nomenclatural changes among some Kansas diatoms. Transactions of the Kansas Academy of Science, 85, 174-176.
Dalu, T., Taylor, J.C., Richoux, N.B., William Froneman, P., 2015. A re-examination of the type material of entomoneis paludosa (W Smith) Reimer and its morphology and distribution in African waters. Journal of the Czech Phycological Society, 15 (1), 11-25.
Dudkowiak, A., Olejarz, B., Łukasiewicz, J., Banaszek, J., Sikora, J. et al., 2011. Heavy metals effect on cyanobacteria Synechocystis aquatilis study using absorption, fluorescence, flow cytometry, and photothermal measurements. International Journal of Thermophysics, 32, 762-773.
Gao, F., Yang, Z.H., Li, C., Zeng, G.M., Ma, D.H. et al., 2015. A novel algal biofilm membrane photobioreactor for attached microalgae growth and nutrients removal from secondary effluent. Bioresource Technology, 179, 8-12.
Geider, R.J., La Roche, J., Greene, R.M., Olaizola, M., 1993. Response of the photosynthetic apparatus of Phaeodactylum tricornatum (Bacillariophycea) to nitrate, phosphate, or iron starvation. Journal of Phycology, 29 (6), 755-766.
Grasshoff, K., Kremling, K., Ehrhardt, M., 2007. Methods of Seawater Analysis: Third, Completely Revised and Extended Edition. Methods Seawater Anal. Third, Complet. Revis. Ext. Ed. 1-600 pp.
Guillard, R., Hargraves, P., 1993. Stichochryis immobilis is a diatom, not a chrysophyte. Phycologia, 32, 234-236.
Helder, W., De Vries, R.T.P., 1979. An automatic phenol-hypochlorite method for the determination of ammonia in seaand brackish waters. Netherlands Journal of Sea Research, 13, 154-160.
Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., Strickland, J.D.H, 1965. Fluorometric determination of chlorophyll. ICES Journal of Marine Science, 30 (1), 3-15.
Isleten-Hosoglu, M., Gultepe, I., Elibol, M., 2012. Optimization of carbon and nitrogen sources for biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content. Biochemical Engineering Journal, 61, 11-19.
Jauffrais, T., Agogué, H., Gemin, M.P., Beaugeard, L., Martin- Jézéquel, V., 2017. Effect of bacteria on growth and biochemical composition of two benthic diatoms Halamphora coffeaeformis and Entomoneis paludosa. Journal of Experimental Marine Biology and Ecology, 495, 65-74.
Jauffrais, T., Drouet, S., Turpin, V., Méléder, V., Jesus, B. et al., 2015. Growth and biochemical composition of a microphytobenthic diatom (Entomoneis paludosa) exposed to shorebird (Calidris alpina) droppings. Journal of Experimental Marine Biology and Ecology, 469, 83-92.
Keskin Gündoğdu, T., Deniz, İ., Çalışkan, G., Şahin, E.S., Azbar, N., 2016. Experimental design methods for bioengineering
applications. Critical Reviews in Biotechnology, 36, 368-388.
Kovárová-Kovar, K., Egli, T., 1998. Growth kinetics of suspended microbial cells: from single-substrate-controlled growth to mixed-substrate kinetics. Microbiology and Molecular Biology Reviews, 62, 646-66.
Kutlu, B., Buyukisik, B., 2014. A modeling using the maximum growth capacity of Hantzschia amphioxys in the Homa Lagoon. African Journal of Biotechnology, 13, 1154-1159.
Lebeau, T., Robert, J.-M., 2003. Diatom cultivation and biotechnologically relevant products. Part II: Current and putative products. Applied Microbiology and Biotechnology, 60, 624-632.
Lomas, M.W., Glibert, P.M., 2000. Comparisons of nitrate uptake, storage, and reduction in marine diatoms and flagellates. Journal of Phycology, 36, 903-913.
Mendes, L.F., Vale, L.A.S., Martins, A.P., Yokoya, N.S., Marinho- Soriano, E. et al., 2012. Influence of temperature, light and nutrients on the growth rates of the macroalga Gracilaria domingensis in synthetic seawater using experimental design. Journal of Applied Phycology, 24, 1419-1426.
Mucko, M., Bosak, S.,Orlić, S.,Gligora, U.M., Peharec, Š.P. et al., 2017. Entomoneis tenera sp. nov., a new marine planktonic diatom (Entomoneidaceae, Bacillariophyta) from the Adriatic Sea. Phytotaxa. 292 (1).
Osada, K., Kobayasi, H., 1985. Fine structure of the brackish water pennate diatom Entomoneis alata (Ehr.) Ehr. var. japonica (Cl.) comb. nov. Japanese Society of Phycology, 33, 215-224.
Paillès, C., Poulin, M., Boudouma, O., Pierre, C., Paddock, M. et al., 2014. Entomoneis calixasini sp . nov ., a new fossil diatom from the Turkish Marmara Sea sediments. Diatom Research, 29, 411-422.
Parsons, T.R., Maita, Y., Lalli, C.M., 1984. Determination of chlorophylls and total carotenoids: spectrophotometric method. A Man. Chemical Biology Methods Seawater Analysis, 101-104.
Poulin, M., Cardinal, A. 1983. Sea ice diatoms from Manitounuk Sound, southeastern Hudson Bay (Quebec, Canada).III. Cymbellaceae, Entomoneidaceae, Gomphonemataceae, and Nitzschiaceae. Canadian Journal of Botany, 61, 107-118.
Sabancı, F.Ç., Koray, T., 2001. İzmir Körfezi ( Ege Denizi ) mikroplanktonunun certikal ve horizontal dağılımına kirliliğin etkisi. Aquatic Sciences, 18, 187-202.
Sarthou, G., Timmermans, K.R., Blain, S., Tréguer, P., 2005. Growth physiology and fate of diatoms in the ocean: A review. Journal of Sea Research, 53, 25-42.
Satoh, A., Vudikaria, L.Q., Kurano, N., Miyachi, S., 2005. Evaluation of the sensitivity of marine microalgal strains
to the heavy metals, Cu, As, Sb, Pb and Cd. Environment International, 31, 713-722.
Soares, B.M., Vieira, A.A., Lemões, J.S., Santos, C.M.M., Mesko, M.F. et al., 2012. Investigation of major and trace element distribution in the extraction-transesterification process of fatty acid methyl esters from microalgae Chlorella sp. Bioresource Technology, 110, 730-734.
Sommer, U., Adrian, R., Bauer, B., Winder, M., 2012. The response of temperate aquatic ecosystems to global warming: Novel insights from a multidisciplinary project. Marine Biology, 159, 2367-2377.
Stefanidou, N., Genitsaris, S., Lopez-Bautista, J., Sommer, U., Moustaka-Gouni, M., 2018. Effects of heat shock and salinity changes on coastal Mediterranean phytoplankton in a mesocosm experiment. Marine Biology, 165.
Strickland, J.D.H., Parsons, T.R., 1972. A Practical Handbook of Seawater Analysis. A Pract. Handbook of Seawater Analysis, 167, 185pp.