In situ experiments on the effect of low pH on the ultrastructure of the seagrasses Cymodocea nodosa and Posidonia oceanica


MARIA KOUTALIANOU
https://orcid.org/0000-0002-5302-0684
MARIA CRISTINA BUIA
https://orcid.org/0000-0002-2370-898X
CHRISTOS KATSAROS
https://orcid.org/0000-0002-7788-3409
Аннотация

The present study investigates the impacts of low pH on the cell structure of the seagrasses Posidonia oceanica (L.) Delile and Cymodocea nodosa (Ucria) Ascherson. The study was applied with in situ experiments at the Castello Aragonese of Ischia (Naples, Italy), where shallow submarine vents, lowering the pH, can be used as natural laboratories. Shoots of the seagrasses were transferred from the control area (pH 8.1) to the two venting areas (pH 7.8 and 6.8) for different times. Epidermal cells of young leaves were examined using transmission electron microscopy (TEM) and tubulin immunofluorescence. After one week at pH 7.8, the cell structure of Posidonia oceanica was normal, while in Cymodocea nodosa microtubule (MT) network and cell structure were affected. In addition, in C. nodosa, ultrastructural analysis revealed a gradual degradation of the nuclei, a disorganization of the chloroplasts, and an increase in the number of mitochondria and dictyosomes. The exposure of both plants for 3 weeks at pH 6.8 resulted in the aggregation and finally in the dilation of the endoplasmic reticulum (ER) membranes. Tubulin immunofluorescence revealed that after three weeks, the MT cytoskeleton of both plants was severely affected. All these alterations can be considered as indications of an apoptotic like programmed cell death (AL-PCD) which may be executed in order to regulate stress response.

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Биографии авторов
MARIA KOUTALIANOU, National and Kapodistrian University of Athens

PhD candidate

National and Kapodistrian University of Athens

Department of Biology

Athens 15784

Hellas (Greece)

MARIA CRISTINA BUIA, Stazione Zoologica Anton Dohrn di Napoli (SZN) Integrative Marine Ecology Department (IME), Branch office ‘Villa Dohrn’ – Benthic Ecology (BLAB), Ischia, (Naples, Italy). Senior Researcher

Stazione Zoologica Anton Dohrn di Napoli (SZN) Integrative Marine Ecology Department (IME), Branch office ‘Villa Dohrn’ – Benthic Ecology (BLAB), Ischia, (Naples, Italy).

Senior Researcher

CHRISTOS KATSAROS, National and Kapodistrian University of Athens

Professor Emeritus

National and Kapodistrian University of Athens

Department of Biology

Athens 15784

Hellas (Greece)