Monitoring of dioxins and related toxic compounds in food of animal origin in Greece

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Published: Nov 10, 2017
DOI: https://doi.org/10.12681/jhvms.14843
Keywords:
dioxins dioxin-like PCBs Greek food mass spectrometry
I. VASSILIADOU (Ε. ΒΑΣΙΛΕΙΑΔΟΥ)
Mass Spectrometry and Dioxin Analysis Laboratory, National Center for Scientific Research "Demokritos"
D. COSTOPOULOU (Δ. ΚΩΣΤΟΠΟΥΛΟΥ)
Mass Spectrometry and Dioxin Analysis Laboratory, National Center for Scientific Research "Demokritos"
L. LEONTIADIS (Λ. ΛΕΟΝΤΙΑΔΗΣ)
Mass Spectrometry and Dioxin Analysis Laboratory, National Center for Scientific Research "Demokritos"
Abstract

Polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and "dioxin-like" polychlorinated biphenyls (PCBs) are environmental pollutants belonging to the persistent organic pollutants (POPs). PCDDs and PCDFs have never been produced intentionally, but they are formed by incomplete combustion of organic material in the presence of chlorine, during industrial processes or intentional and unintentional burning of solid waste. PCBs have been produced industrially as mixtures and have been used in various applications, though their production has been banned since the 1970's. Their toxicity is mediated through the interaction with the aryl hydrocarbon (AH) receptor, causing severe health effects to humans after chronic exposure, which include developmental and immunological effects, carcinogenicity and chloracne. More than 90% of the daily human intake is due to food ingestion and especially, to the consumption of fat-containing animal food products, due to the lipophilic nature of these compounds. In order to control food contamination by these compounds, the European Union has set maximum limits for PCDD/Fs and PCBs in food products (EU Regulation 1881/2006/EC). The Mass Spectrometry and Dioxin Analysis Laboratory of "NCSR" Demokritos has been operating since 2002 and it performs analysis of PCDD/Fs and PCBs in food, feed, biological and environmental samples, applying international protocols of analysis (EPA 1613, EPA 1668), based on appropriate sample clean-up and determination by high resolution mass spectrometry (HRMS), using the isotope dilution method, as specified by the EU Regulation 1883/2006/EC. The Laboratory has been accredited according to ISO/IEC 17025 by ESYD and it is the National Reference Laboratory of the European Commission for Greece and Cyprus. Here we present here a comprehensive report of the activities of the Mass Spectrometry and Dioxin Analysis Laboratory during its operation, concerning the monitoring of dioxins and similar toxic compounds in food of animal origin in Greece. Through collaboration with the official bureaus of food, environment and health control organizations of Greece, the Laboratory has conducted analysis in a large number of food and animal feed products sampled randomly in the Greek market. The results are well below the EU maximum levels and, in general, lower than those monitored in other European and Mediterranean countries. This has been attributed to the fact that Greece is not highly industrialized and has never produced PCBs and does not perform systematic waste incineration. On the other hand, samples have been collected and analysed from areas suspected for dioxin contamination, such as areas affected by accidental fires, landfills or exposed to industrial pollution. In some of these cases, unusually high levels of dioxin contamination in food samples of animal origin have been observed, in some cases above EU limits, and appropriate measures have been taken by Greek authorities to prevent their consumption and to restore normal dioxin levels.

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References
ATSDR (2002). ATSDR Tox Profiles, Atlanta, 2002. http://www.atsdr.cdc.gov [accessed 6 June 2011]
Bascompta O, Montana MJ, Marti R, Broto-Puig F, Cornelias L, Diaz-Ferrero J, Rodriguez-Larena MC (2002) Levels of persistent organic pollutants (PCDD/F and dioxin-like PCB) in food from the Mediterranean diet. Organohalogen Compounds 57:149.
Bencko V (2003) Risk assessment and human exposure to endocrine disrupters. In: Jedrychovski WA, Petera FP, Maugeri U (Eds.), Molecular Epidemiology in Preventive Medicine. International Center for Studies and Research in Biomedicine in Luxembourg: pp. 315-327.
Birnbaum LS, Tuomisto J (2000) Non-carcinogenic effects of TCDD in animals. Food Addit Contam 17: 275-288.
Bleeker I, Fischer AB, Tilkes F, Eikmann Τ (1999) PCB Konzentrationen im menschlichen Blut. Umwelt Forsch Prax 4: 84-96.
Bocio A, Llobet JM, Domingo JL, Casas C, Teixido A, Muller L (2002) Levels of PCDD/PCDFs in food samples from Catalonia, Spain. Organohalogen Compounds 57:105.
Bordajandi LR, Herrero L, Abad E, Rivera J, Gonzalez M J (2002) Presence of PCDDs, PCDFs and PCBs in selected food samples from Huelva (Spain). Organohalogen Compounds 57: 69.
Costopoulou D, Vassiliadou I, Papadopoulos A, Makropoulos V, Leondiadis L. Chemosphere (2006) Levels of dioxins, furans and PCBs in human serum and milk of people living in Greece. Chemosphere 65:1462-9.
Dencker L (1985) The role of receptors in 2,3,7,8-tetrachlorodibenzop-dioxin (TCDD) toxicity. Arch Toxicol Suppl 8: 43-60.
EFSA Journal (2010) 8:1701-35.
Fernandes A, Mortimer D, Gem M, Rose M (2010) Dioxins and PCBs in Offal: occurrence and dietary exposure. Chemosphere 81: 536-540.
Focant J-F, Eppe G, Pirard C, Massart Α-C, Andre J-E, De Pauw E (2002) Levels and congener distributions of PCDDs, PCDFs and non-ortho PCBs in Belgian foodstuffs. Assessment of dietary intake. Chemosphere 48:167-179.
Froescheis O, Looser R, Cailliet GM, Jarman WM, Ballschmiter Κ (2000) The deepsea as a final global sink of semivolatile persistent organic pollutants? Part 1. PCBs in surface and deepsea dwelling fish of North and South Atlantic and the Monterey Bay Canyon (California). Chemosphere 40, 651-660.
Hosseinpour J, Rottler Η, Joas R, Potrykus A, Schott R (2002) Dioxinlike PCB levels in feed and food from across the European Union. Organohalogen Compounds 57: 77.
Leondiadis L, Costopoulou D, Vassiliadou I, Papadopoulos A (2008) Monitoring of dioxins and dioxin-like PCBs in food, feed and biological samples in Greece. In: The Fate of Persistent Organic Polluntants in the Environment. Springer Ed: pp 83-98.
Liem AK, Fürst Ρ, Rappe C (2000) Exposure of populations to dioxins and related compounds. Food Additives and Contaminants 17:241-259.
Liem AKD, Theelen RMC (1997) Dioxins: chemical analysis, exposureand risk assessment. Thesis, National Institute of Public Health and the Environment, Bilthoven, The Netherlands, ISBN 90-393-2012-8.
Linden J, Lensu S, Tuomisto J, Pohjanvirta R (2010) Dioxins, the aryl hydrocarbon receptor and the central regulation of energy balance. A review. Frontiers in Neuroendocrinology 31: 452-478.
Martens D, Balta-Brouma K, Brotsack R, Michalke B, Schramel P, Klimm C, Henkelmann Β, Oxynos Κ, Schramm K-W,
Diamadopoulos E, Kettrup A (1998) Chemical impact of uncontrolled solid waste combustion to the vicinity of the
Kouroupitos ravine, Crete, Greece. Chemosphere 36: 2855-2866.
McKay G (2002) Dioxin characterization, formation and minimization during municipal solid waste (MSW) incineration: review. Chemical Engineering Journal 86: 343-368.
Papadopoulos A, Vassiliadou I, Costopoulou D, Papanicolaou C, Leondiadis L (2004) Levels of dioxins and dioxin-like PCBs in food samples on the Greek market. Chemosphere 57:413-9.
Poellinger L (2000) Mechanistic aspects - the dioxin (aryl hydrocarbon) receptor. Food Additives and Contaminants 17 (4): 261-266.
Pohjanvirta R, Tuomisto J (1994) Short-term toxicity of 2,3,7,8- tetrachlorodibenzo-p-dioxin in laboratory animals: effects, mechanisms and animal models. Pharmacol Rev 46: 483-549.
Porta M, Zumeta E (2002) Implementing the Stockholm treaty on POPs (Editorial). Occupational & Environmental Medicine 59:651-652.
Quaß U, Fermann M, Broeker G (2004) The European dioxin air emission inventory project - Final results. Chemosphere 54:1319-1327.
Rudel RA, Seryak LM, and Brody JG (2008) PCB-containing wood floor finish is a likely source of elevated PCBs in resident's blood, household air and dust: a case study of exposure. Environmental Health 7: 2-9.
Ruokojarvi P, Aatamila M, Ruushanen J (2000) Toxic chlorinated andpolyaromatic hydrocarbons in simulated house fires. Chemosphere 41: 825-828.
Schramm K-W, Kaune A, Lehnardt R, Hofmaier A, Henkelmann Β, Kettrup A (1998) Isokinetic sampling of PCFF/F response in low and high volatile fractions of a wood incinerator. Organohalogen Compounds 36: 289-292.
Startin JR, Rose MD (2003) Dioxins and dioxin-like PCBs in food. In: Schecter A, Gasiewicz TA (Eds.), Dioxins and Health. Wileylnterscience, New York, USA: pp. 89-136.
Van den Berg M, Birnbaum L, Bosveld AT, Brunstrom Β, Cook Ρ, Feeley M, Giesy JP, Hanberg A, Hasegawa R, Kennedy SW, Kubiak Τ, Larsen JC, van Leeuwen FX, Liem AK, Nolt C, Peterson RE, Poellinger L, Safe S, Schrenk D, Tillitt D, Tysklind M, Younes M, Waern F, Zacharewski Τ (1998) Environmental Health Perspectives 106: 775-792.
Van den Berg M, Birnbaum LS, Denison M, De Vito M, Farland W, Feeley M, Fiedler H, Hakansson H, Hanberg A, Haws L, Rose M, Safe S, Schrenk D, Tohyama C, Tritscher A, Tuomisto J, Tysklind M, Walker Ν, Peterson RE (2006) The 2005 World Health Organization re-evaluation of human and mammalian toxic equivalency factors for dioxins and dioxin-like compounds. Toxicol Sci 93: 223-241.
Van Leeuwen FX, Feeley M, Schrenk D, Larsen JC, Farland W, Younes M (2000) Dioxins: WHO's tolerable daily intake (TDI) revisited. Chemosphere 40:1095-101.
Vassiliadou I, Papadopoulos A, Costopoulou D, Vasiliadou S, Christoforou S, Leondiadis L (2009) Dioxin contamination after an accidental fire in the municipal landfill of Tagarades, Thessaloniki, Greece. Chemosphere 74:879-84.
Walker B, Cooper C (1992). Air pollution emission factors for medical waste incinerators. Journal of the Air and Waste Management Association 42: 784-789.