| More

The development of a DNA vaccine for the Spring Viraemia of Carp Virus (SVCV)

Views: 54 Downloads: 38


DNA immunization is a promising approach to induce immunity/protection against infectious agents. In the present study a DNA vaccine to Spring Viraemia of Carp Virus (SVCV) G glycoprotein was investigated. The G gene was cloned intact with several promoters namely: 1) the immediate early promoter/enhancer of the human cytomegalovirus; 2) the simian virus 40 promoter; and 3) the porcine myosin heavy chain IIA promoter. In fusion constructs, the G gene was fused either upstream to the LacZ gene or downstream to the murine Ig k-chain leader sequence. BALB/c mice immunized with the aforementioned plasmids produced strong T-cell proliferative responses to the SVCV, in the absence of any detectable antibody response. The group that responded most robusdy was the one that had received the LacZ fusion gene and therefore, this construct was further tested in mice with the addition of a plasmid expressing the murine cytokine GMCSF. Co-administration of the GMCSF expressing plasmid resulted in high SVCV spleenoproliferative responses in mice and the development of antibodies to both fused proteins. These results indicate the functionality of the constructs and the possibility of giving protection in fish.


Spring Viraemia of Carp Virus; DNA vaccine; Fish

Full Text:



Anderson ED, Mourich DV, Fahrenkrug SC, La Patra S, Shepherd J, Leong JA. Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus. Molecular Marine Biology and Biotechnology 1996, 5: 114-122.

Anderson ED, Mourich DV, Leong JAC. Gene expression of rainbow trout (Oncorynchus mykiss) following intramuscular injection of DNA. Molecular Marine Biology and Biotechnology 1996,5:105-113.

Andre S, Seed B, Eberle J, Scharaut W, Bultmann A, Haas J. Increased immune response elicited by DNA vaccination with a synthetic gpl20 sequence with optimized codon usage. Journal of Virology 1998, 72:1497-1503.

Barry MA, Johnston SA. Biological feature of genetic immunization. Vaccine 1997,15:788-791.

Chang KC Critical regulatory domains in intron 2 of a porcine sarcomeric myosin heavy chain gene. J Muscle Res Cell Motil. 2000; 21(5):451-61.

Coll JM. The glycoprotein G of rhabdoviruses. Archives of Virology 1995,140:827851.

Czerkinsky C, Moldoveanu Z, Mestecky J, Nilsson LA, Ouchterlony O, Tarkowski, A. A novel two color ELISPOT assay. 1. Simultaneous detection of distinct types of antibody-secreting cells. Journal of Immunological Methods 1988,115:31-37.

Davis HL, Brazlot Millan CL, Watkins SC. Immune mediated destruction of transfected muscle fibers after direct gene transfer with antigen expressing plasmid DNA. Gene Therapy 1997, 4:181-188.

Dixon P. Immunization with viral antigens: viral diseases of carp and catfish. Development of Biological Standardization 1997, 90:221-232.

Dixon PF, Hattenberger-Baudouy AM, Way K. Detection of carp antibodies to spring viraemia of carp virus by a competitive immunoassay. Diseases of Aquatic Organisms 1994,19:181-186.

Donnelly JJ, Ulmer JB, Shiver WS, Liu MA. DNA Vaccines. Annual Reviews of Immunology 1997, 15: 617-618.

Fijan N. Vaccination against Spring Viraemia of Carp Virus. Fish Vaccination. Academic press limited 1988, pp. 204-215.

Fynan EF, Robinson HL, Webster RG. Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine. DNA and Cell Biology 1993,12:785-789.

Gerloni M, Lo D, Ballou WR, and Zanetti M. Immunological memory after somatic transgene affected by priming with GMCSF and does not require bone marrow-derived dendritic cells. European Journal of Immunology 1998, 28: 1832-1838.

Grifantini R, Fineo Ο, Bartolini E, Draghi M, Del Giudice G, Kocken C, Thomas A, Abrigiani S, Grandi G. Multi-plasmid DNA vaccination avoids antigenic competition and enhances immunogenicity of a poorly immunogenic plasmid. European Journal of Immunology 1998, 28:1225-1232.

Hansen E, Fernandes K, Goldspink G, Butterworth P, Umeda PK, Chang KC. Strong expression of foreign genes following direct injection into fish muscle. FEBS Letters 1991, 290: 73-76.

Kanellos T., Sylvester ID., D'Mello F, Howard CR, Mackie A, Dixon P, Chang K, Ramstad A Midtlyng P, Russell P. DNA vaccination can protect Cyprinus Carpio against spring viraemia of carp virus. Vaccine 2006, 24: 4927-4933.

Leong JC, Anderson E, Bootland LM, Chiou PW, Johnson M, Kim C, Mourich D, and Trobridge G. Fish vaccines antigen produced or delivered by recombinant DNA technologies. Development of Biological Standardization 1997, 90: 267-278.

Lorenzen N, Lorenzen K, Einer-Jensen K, Heppel J, Wu T, Davis H. Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination. Fish and Shellfish Immunology 1998, 8: 261-270.

Martins PL, Lau LL, Asano MS, Ahmad R. DNA vaccination against persistent viral infection. Journal of Virology 1995, 69:2574-2582.

Mitchison NA. The carrier effect in the secondary response to hapten-protein conjugates. European Journal of Immunology 1971, 1: 10-17.

Oliveira CD, Feenstra A, Vos H, Osterhaus AD, Shiels BR, Cornelissen AW, Jongejan F. Induction of protective immunity to Theileria annulata using two major surface antigen presented

by different delivery systems. Vaccine 1997, 16:1796-1804.

Partidos CD, Stewart MW. Prediction and identification of a T-cell epitope in the fusion protein of measles virus immunodominant in mice and humans. Journal of General Virology 1990, 71: 2099-2105.

Roberts AM. Spring viraemia of carp virus. The Veterinary Record 1994,11: 694.

Robertson J S. Safety considerations for nucleic acid vaccines. Vaccine 1994,12: 1526-1528.

Robinson HL. Nucleic acid vaccines: an overview. Vaccine 1997,15:785-787.

Russell PH, Kanellos T, Sylvester ID, Chang KC, Howard CR. Nucleic acid immunisation with a reporter gene results in antibody production in goldfish (Carassius auratus L.). Fish and Shellfish Immunology 1998, 8:121-128.

Russell PH. The non-specific stimulation of avian peripheral blood lymphocytes from uninfected chickens by paramyxoviruses and influenza viruses. Veterinary Microbiology 1988, 16: 181-188.

Sambrook J, Fritsch EF, Maniatis T. Molecular cloning. A laboratory Manual. 2nd edition. Cold Spring Harbor Press 1989.

Tighe H, Corr M, Roman M, Raz E. Gene vaccination: plasmid DNA is more than just a blueprint. Immunology Today 1998,19:89-97.

Wells DJ. Gene transfer by intramuscular injection of plasmid DNA. Molecular and Cell Biology of human Gene Therapeutics, ed. G Dickson, pp 83-103. Chapman and Hall, London 1995.

Wells KE, Maule J, Kingston R, Foster K, McMahon J, Damien E, Poole A, Wells DJ. Immune responses, not promoter inactivation, are responsible for decreased long term expression following plasmid gene transfer into skeletal muscle. FEBS Letters 1997, 407: 164-168.

Xiang ZQ, Spitalnik SL, Cheng J, Erikson J, Wojczyk B, and Erti HCJ. Immune responses to nucleic acid vaccines to rabies virus. Virology 1995, 209: 569-579.


  • There are currently no refbacks.