Comparative study on Pharmacokinetic Profiles of Chicken IgY to Horse IgG in Rabbits

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Published: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15524
Keywords:
tetanus IgY IgG pharmacokinetics intravenous administrations intramuscular administrations
ZHAOXIANG ZHOU
State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science
JIAN WANG
College of Biological Science and Engineering, Shaanxi University of Technology
SONG GAO
College of Veterinary Medicine, Northwest A&F University
HAO REN
College of Veterinary Medicine, Northwest A&F University
WENYAN XIE
College of Veterinary Medicine, Northwest A&F University
DIRAVIYAM THIRUMALAI
School of Chemical and Biotechnology, SASTRA University,
XIAOYING ZHANG
State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science; College of Veterinary Medicine, Northwest A&F University
Abstract

Chicken IgY antibody has been extensively reported for various applications as an alternative to mammalian IgG. To evaluated the pharmacokinetics profile of chicken IgY in comparison with horse IgG. Chicken IgY antibody was prepared by immunizing the white leghorn chicken with tetanus toxoid (TT) and then extracted anti-TT-IgY from immune egg yolks by PEG-6000 extraction. The titer of anti-TT-IgY was determined by ELISA in order to select the hyper immune eggs for further extraction. Rabbits were injected with 300 μg/kg of Chicken IgY and horse IgG by intravenous (i.v.) and intramuscular (i.m.) administrations. Then, the concentrations of IgY and IgG in rabbit serum were determined using indirect ELISA. Pharmacokinetic parameters were estimated using non-compartmental analysis. IgY can reach higher concentration (Cmax= 4.54 mg/L after i.m. injection) within shorter time (Tmax= 0.12 h after i.m. injection) than IgG (Cmax= 3.99 ng/mL after i.m. injection; Tmax=0.11 h after i.m. injection), while the IgY lifespan (T1/2=26.98 h and 31.98 h after i.v. and i.m. administration, respectively) in the body was comparable with IgG (T1/2=27.94 h and 29.58 h after i.v. and i.m. administration, respectively). IgY might be a promising choice as an alternative to mammalian sourced antibodies.

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References
Araujo AS, Lobato ZIP, Chavez-Olortegui C, Velarde DT (2010) Brazilian IgY-Bothrops antivenom: Studies on the development of a process in chicken egg yolk. Toxicon : official journal of the International Society on Toxinology 55:739-44.
Behring EV, Kitasato S (1890) Ueber das Zustandekommen der Diphtherie-Immunitӓt und der Tetanus-Immunitӓt bei Thieren. Deutscbe Medizhiscbe Wocbenschift 16:1113-4.
de Almeida CMC, da Silva CL, Couto HP, Escocard RDM, da Rocha DG, Sentinelli LD, et al (2008) Development of process to produce polyvalent IgY antibodies anti-African snake venom. Toxicon : official journal of the International Society on Toxinology 52:293-301.
Diaz P, Malave C, Zerpa N, Vazquez H, D’Suze G, Montero Y, et al (2014) IgY pharmacokinetics in rabbits: Implications for IgY use as antivenoms. Toxicon : official journal of the International Society on Toxinology 90:124-33.
Guimarães MCC, Amaral LG, Borges FV, Vieira HPL (2009) Considerations on the stability of IgY antibodies anti-tetanus toxoid. R Ci Méd Biol 8:307-14.
He JX, Thirumalai D, Schade R, Zhang XY (2014) Chronobiologicalstudies of chicken IgY: Monitoring of infradian, circadian and ultradian rhythms of IgY in blood and yolk of chickens. Vet Immunol Immunop 160:266-72.
Hertz MA, Sørensen SM (2014) Tetanus can still be found in Denmark. Ugeskr Laeger 176:V06140334.
Huang P, Tan S, Zhang YX, Li JS, Chai C, Li JJ, et al (2014) The effects of wine-processing on ascending and descending: the distribution of flavonoids in rat tissuesafter oral administration of crude and wine-processed Radix scutellariae. J Ethnopharmacol 155:649-64.
Klemperer F (1893) Über natÜrliche Immunitӓt und ihre Verwerthung fÜr Immunisirungs- therapie. Arch Exp Path Pharmak 31:356-82.
Keller MA, Stiehm ER (2000) Passive immunity in pr evention and treatment of infectious diseases. Clinical microbiology reviews 13:602-14.
Kiem TX (2000) The production of Calloselasma rhodostoma antivenom (C.R.A.V.) from egg yolk of hens immunized with venom: its application for treatment of snake bite patients in Vietnam. In: Proceedings of the XIIIth World Congress of the International Society of Toxinology International Society of Toxinology, Paris.
Luo SD (2002) Human tetanus immune globulin and its application. Journal of Emergenoy Medicine 11:285-6.
Meshad AHE, Ibrahim EM, Selim AM, Hamouda FK (2013) A New Trend in Donkeys Tetanus Treatment Using IgY. Researcher 5:23-9.
Murai A, Murota R, Doi K, Yoshida T, Aoyama H, Kobayashi M, et al (2013) Avian IgY is selectively incorporated into the egg yolks of oocytes by discriminating Fc amino acid residues located on the Cu3/Cu4 interface. Dev Comp Immunol 39:378-87.
Meenatchisundaram S, Parameswari G, Michael A, Ramalingam S (2008) Studies on pharmacological effects of Russell’s viper and Saw-scaled viper venom and its neutralization by chicken egg yolk antibodies. International Immunopharmacology 8:1067-73.
Polson A, von Wechmar MB, van Regenmortel MH (1980) Isolation of viral IgY antibodies from yolks of immunized hens. Immunol Commun 9:475-93.
Paul K, Manjula J, Deepa EP, Selvanayagam ZE, Ganesh KA, Rao PVS (2007) Anti-Echis carinatus venom antibodies from chicken egg yolk: Isolation, purification and neutralization efficacy. Toxicon : official journal of the International Society on Toxinology 50:893–900.
Spillner E, Braren I, Greunke K, Seismann H, Blank S, du Plessis D (2012) Avian IgY antibodies and their recombinant equivalents in research, diagnostics and therapy. Biologicals : journal of the International Association of Biological Standardization 40:313-22.
Sun HC, Chen SR, Cai X, Xu GY, Qu LJ (2013) Correlation analysis of the total IgY level in hen serum, egg yolk and offspring serum. J Anim Sci Biotechno 4(1):253-256.
Smith JW, MacIver AG (1969) Studies in experimental tetanus infection. Journal of medical microbiology 2:385-93.
Schade K, Calzado EG, Sarmiento R, Chacana PA, Porankiewicz-Asplund J, Terzolo HR (2005) Chicken egg yolk antibodies (IgY-technology): A review of progress in production and use in research and human and veterinary medicine. Atla-Altern Lab
Anim 33:129-54.
Shen YZ, Zhang YX (2012) Scientific treatment and prevention of tetanus. Shanghai Medical & Pharmaceutical Journal 19:9-12.
Thalley BS, Carroll SB (1990) Rattlesnake and scorpion antivenoms from the egg yolks of immunized hens. Bio/technology 8:934-8.
Theakston RD, Warrell DA (1991) Antivenoms: a list of hyperimmune serra currently available for the treatment of environment by bites and stings. Toxicon : official journal of the International Society on Toxinology 29:1419-70.
Vázquez H, Chávez-Haro A, García-Ubbelohde W, Paniagua-Solís J, Alagón A, Sevcik C (2010) Pharmacokinetics of a F(ab’)2 scorpion antivenom administered intramuscularly in healthy human volunteers. International Immunopharmacology 10:1318-24.
Zolfagharian H, Dounighi NM (2015) Study on development of Vipera lebetina snake antivenom in chicken egg yolk for passive immunization. Human vaccines & immunotherapeutics 11(11):2734-2739.
Zhang J, Zheng QS (2012) DAS software user manual 7-12.