Changes in oxidative stress markers during electro-ejaculation procedure in merino rams


Published: Jan 29, 2018
Keywords:
Merino rams electro-ejaculation oxidative stress physiological parameter hematological parameters
A. F. FIDAN
D. YENI
F. AVDATEK
Ü. ÖZÇINAR
Ö. HAZMAN
Abstract

Electro-ejaculation is a technique used to collect semen from rams. The aim of the study was to evaluate the stress response and the oxidant/antioxidant levels during electro-ejaculation (EE) procedure in merino rams. To assess the effect of this technique, six 3-4 years old merino rams were subjected to semen collection by EE. The technique was applied one time in previously untreated animals. The studied parameters [Heart and respiratory rate, rectal temperature, white blood cell count (WBC), red blood cell count (RBC), hemoglobin (Hb) levels, hematocrit (Hct) value, blood malondialdehyde (MDA) and glutathione (GSH) concentrations, plasma cortisol, nitric oxide (NO), glucose, cholesterol and triglyceride concentrations as well as plasma total antioxidant status (TAS) and total oxidant status (TOS)] were measured before and immediately after electrical stimulation. Heart and respiratory rate, WBC, MDA, glucose, cholesterol and triglyceride concentrations as well as plasma TOS and cortisol concentrations were dramatically increased after EE procedure, whereas rectal temperature, TAS and GSH concentrations were significantly decreased. These results demonstrate that MDA, GSH, TAS and TOS were the most powerful markers for evaluating the oxidant/antioxidant status in merino rams. Furthermore, EE procedure was a stressful situation leading to an oxidative stress, which can be amplified by increased glucocorticoid secretion.

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References
Aleksandrovskii IA, Poıurovskii MV, Neznamov GG, Seredeniia SB, Krasova EA (1998) Lipid peroxidation in emotional stress and neurotic disorders. Zhurnal Nevropatologii i Psikhiatrii Imeni S.S. Korsakova 88(11): 95-101.
Avcı G, Kucukkurt I, Fidan AF, Eryavuz A, Aslan R, Dundar Y (2008) The influence of vitamin C and xylazine on cortisol, lipid peroxidation and some biochemical parameters in transported sheep. Fırat University Journal of Health Sciences (Veterinary) 22(3):147-152.
Beutler E, Dubon OB and Kelly M (1963) Improved method for the determination of blood glutathione. Lab Clin Med 61:882-8.
Boussena S, Bouaziz O, Dehimi L, Hireche S, Aimeur R, Kabouia R (2001) The effects of electroejaculation on some physiological parameters (rectal temperature,respiratory and cardiac rates) in OuledDjellal breed. Slovak J Anim Sci 46(1):16-21.
Brindley GS (1981) Electroejaculation: its technique, neurological implications and uses. J Neurol Neurosurg Psychiatry 44:9-18.
Cingi, C.C. Durmus FB, Yasemin SK, Fidan AF (2012) Stress Response in Dairy Cows Related to Rectal Examination. Acta Scientiae Veterinariae 40:3 1053.
Damián JP, Ungerfeld R (2011) The stress response of frequently electroejaculated rams to electroejaculation: hormonal, physiological, biochemical, haematological and behavioural parameters. Reprod DomestAnim 46: 646–650.
Dantzer R, Mormède P (1983) Stress in farm animals: A need for a reevaluation. J Anim Sci 57:1 6-18.
Draper HH, Hardley M (1990) Malondialdehyde determination as index of lipid peroxidation. Meth Enzymol 186: 421-431.
Emecen Ö, Berçik Inal B, Erdenen F, Usta M, Aral H, Güvenen G (2010) Evaluation of oxidant/antioxidant status and ECP levels in asthma. Turk J Med Sci 40(6): 889- 895. doi:10.3906/sag-0906-6
Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 38:1103–1111.
Fidan AF, Küçükkurt İ, Eryavuz A, Ciğerci İH, Yardimci M, Ozdemır A (2009) Effects of shearing procedures on oxidant-antioxidant status in Chios sheep. Revue Méd Vét 160(7):349-355.
Fidan AF, Pamuk K, Ozdemir A, Saritas ZK, Tarakci U (2010) Effects of dehorning by amputation on oxidantantioxidant status in mature cattle. Revue Méd Vét 161(11):502-508.
Fumagalli F, Villagra´ N M, Damián J.P, Ungerfeld R (2012) Physiological and Biochemical Parameters in Response to Electroejaculation in Adult and Yearling Anesthetized Pampas Deer (Ozotocerosbezoarticus) Males. Reprod Domest Anim 47(2):308–312.
Ghiselli A, Serafini M, Natella F, Scaccini C (2000) Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radic Biol Med 29:1106–1114.
Granger DA, Hibel LC, Fortunato CK, Kapelewski CH (2009) Medication effects on salivary cortisol: tactics and strategy to minimize impact in behavioral and developmental science. Psychoneuroendocrinology 34(10):1437-48.
Kagan AR, Levi L (1974) Health and environment – psychosocial stimuli: a review. Social Science and Medicine 8:225-241.
Kayar A, Dokuzeylul B, Kandemir F M, Kirbas A, Bayrakal A, Or M E (2015) Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus. Veterinarni Medicina 60(5):274–281
Lindsay RD (1969) Sexual activity and semen production of rams at high temperatures. J Reprod Fert 18:1-8.
Marco I, Lavin S (1999) Effect of the method of capture on the haematology and blood chemistry of red deer (Cervuselaphus). Res Vet Sci 66:81-84.
Minka NS, Ayo JO (2009) Physiological responses of food animals toroad transportation stress. Afr J Biotechnol 8:7415-7427.
Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5:62-71.
Moberg GP (1987) Problems in defining stress and distress in animals. J Am Vet Med Assoc 191(10):1207-1211.
Olvera JRL (2004) Capture, restraint and transport stress in Southern chamois (Rupicaprapyrenaica). Modulation with acepromazine and evaluation using physiological parameters. PhD Thesis; UniversitatAutònoma de Barcelona. Barcelona, Spain.
Ortiz-De-Montellano M, Galindo-Maldonado F, Cavazos-Arizpe EO,Aguayo-Arceo AM, Torres-Acosta JFJ, Orihuela A (2007) Effect of electro-ejaculation on the serum cortisol response of Criollo goats (Capra hircus). Small Rum Res 69:228–231.
Orzechowski A, Ostaszewski P, Brodnicka A, Wilczak J, Jank M, Balasińska B, Grzelkowska K, Ploszaj T, Olczak J, Mrówczyńska A (2000) Excess of glucocorticoids impairs whole-body antioxidant status in young rats-relation to the effect of dexamethasone in soleus muscle and spleen. Horm Metab Res 32;174-180.
Peinado V I, Fernandez-Aries A, Viscor G, Palomeque J (1993) Haematology of Spanish ibex (Capra pyrenaicahispanica) restrained by physical or chemical means. Vet Rec 132: 580-583.
Pryor WA, Houk KN, Foote CS, Fukuto JM, Ignarro LJ, Squadrito GL, Davies KJ (2006) Free radical biology and medicine: it’s a gas, man!. Am J Physiol Regul Integr Comp Physiol 291(3):491-511.
Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S and Dhama K (2014) Oxidative Stress, Prooxidants, and Antioxidants: The Interplay. Bio Med Research International 1-19. doi:10.1155/2014/761264.
Reuter S, Subash CG, Madan MC, and Bharat BA (2010) Oxidative stress, inflammation, and cancer: How are they linked? Free Radic Biol Med 49(11):1603–1616
Rijnberk AD, Mol JA (1997) Adrenocortical function. In: Clinical biochemistry of domestic Animals. 5th edition, Kaneko J.J., Harvey J.W. and Bruss M.L. (eds.), Academic Press Inc., San Diego, USA: pp 553-570.
Shaw DF, Tume KR (1992) The assessment of pre-slaughter treatments of livestock by measurement of plasma constituents: A review of recent work. Meat Sci 32:311-329.
Whitlock BK, Coffman EA, Coetzee JF, Daniel JA (2012) Electroejaculation increased vocalization and plasma concentrations of cortisol and progesterone, but not substance P, in beef bulls. Theriogenology 78:737–746.
Wu G, Fang YZ, Yang S, Lupton JR, Turner ND (2004). Glutathione Metabolism and Its Implications for Health. The Journal of nutrition 134:489-92.
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