The Effect of Lyophilized Pomegranate Extract on Epididymal Sperm Quality, Oxidative Stress and Spermatogenic Cell Density in Rabbits

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Published: May 2, 2018
DOI: https://doi.org/10.12681/jhvms.16384
Keywords:
Oxidative stress Pomegranate extract Rabbit Sperm characteristics Spermatogenic cell density
F. AVDATEK
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Afyon Kocatepe University
D. YENI
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Afyon Kocatepe University
D. F. BAŞER
Department of Internal Medicine, Faculty of Veterinary Medicine, Afyon Kocatepe University
H. H. DEMIREL
Department of Laborant and Veterinary Health, Bayat Vocational School, Afyon Kocatepe University
Ö. HAZMAN
Department of Chemistry, Biochemistry Division, Faculty of Science and Arts, Afyon Kocatepe University
T. CIVELEK
Department of Internal Medicine, Faculty of Veterinary Medicine, Afyon Kocatepe University
Abstract
The aim of this study was to investigate the effect of lyophilized pomegranate extract on spermatological features, pathology of testes and total antioxidant/oxidant status in rabbits. Adult male rabbits were divided into four groups containing six rabbits each. For 8 weeks, Group I received standard diet and 1 ml % 0.5 carboxymethyl cellulose (CMC), Group II received 25 mg/kg/day pomegranate extract + 1 ml % 0.5 carboxymethyl cellulose (CMC), Group III received 50 mg/kg/day pomegranate extract + 1 ml % 0.5 CMC and Group IV received 100 mg/kg/day pomegranate extract + 1 ml % 0.5 CMC. Rabbits were sacrificed by using xylazine 5 mg/kg+ketamine 35 mg/kg anaesthesia and were euthanized by 150 mg / kg intraperitoneal thiopental sodium at the end of the eighth week. Spermatozoon motility, abnormal sperm rate, sperm membrane integrity, total antioxidant/oxidant level and spermatogenic cell density were investigated. All analyses were done only once at the end of study period. Data were compared by analysis of variance (ANOVA) and the degree of significance was set at (p<0.05). Sperm motility and membrane integrity increased significantly (p<0.05) in groups II, III and IV; abnormal sperm rate decreased significantly (p<0.05) in groups III, IV; total oxidant status decreased significantly (p<0.05) in group IV in comparison to the control group. Seminiferous tubule diameter increased significantly (p<0.05) in all groups compared to the control group. Germinal cell layer thickness significantly increased (p<0.05) in group IV compared to the control group. Results of this study suggest that 50mg/kg/day + 1 ml % 0.5 CMC and 100 mg/kg/day + 1 ml % 0.5 CMC improve sperm parameters in rabbits.
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References
Afaq F, Saleem M, Krueger CG, Reed JD, Mukhtar H. (2005) Anthocyanin- and hydrolyzable tannin-rich pomegranate fruit extract modulates MAPK and NF-??B pathways and inhibits skin tumorigenesis in CD-1 mice. Int J Cancer113: 423–33.
Ahmed MAE, El Morsy EM, Ahmed AAE. (2014) Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats. Life Sci 110: 61–9.
Aitken RJ, Sawyer D. (2003) The Human Spermatozoon -Not Waving but Drowning. In: Robaire B, Hales BF, editors. Adv. Male Mediat. Dev. Toxic., Boston, MA: Springer US p. 85–98.
Çam M, Hişil Y, Durmaz G. (2009) Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food Chem 112:721–6.
De Nigris F, Williams-Ignarro S, Lerman LO, Crimi E, Botti C, Mansueto G, et al. (2005) Beneficial effects of pomegranate juice on oxidation sensitive genes and endothelial nitric oxide synthase activity at sites of perturbed shear stress. Proc Natl Acad Sci U S A 102:4896–901.
Dinkova-Kostova AT, Talalay P. (2008) Direct and indirect antioxidant properties of inducers of cytoprotective proteins. Mol Nutr Food Res 52: 128–38.
Ducci M, Gaazano A, Vıllanı C, Cela V, Artını PG, Martellı F, Genazzanı AR. (2002) Membrane integrity evaluation in rabbit spermatozoa. Eur. J. Obstet. Gynaecol. Reprod. Biol 102: 53-56.
Erel O. (2005) A new automated colorimetric method for measuring total oxidant status. Clin Biochem 38:1103–1111.
Erel O. (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 37:277–285.
Fayed AM, Azoz AA, Zedan AH, Basyony M. (2012) Effects of Pomegranate Peel As Antıoxıdant Supplementatıon On Dıgestıbılıty, Blood Bıochemıcal And Rabbıt Semen Qualıty. Egyptian J. Nutrition and Feeds 15(2): 343-354.
Fedder MDK, Jakobsen HB, Giversen I, Christensen LP, Parner ET, Fedder J. (2014) An extract of pomegranate fruit and galangal rhizome increases the numbers of motile sperm: a prospective, randomised, controlled, double-blinded trial. PLoS One 9(10): 2-9.
Fuhrman B, Volkova N, Dornfeld L, Rosenblat M, Kaplan M, Hayek T, et al. (2000) Pomegranate juice consumption reduces oxidative stress and low density lipoprotein atherogenic modifications: studies in the atherosclerotic apolipoprotein E deficient mice and in humans. Am J Clin Nutr 151:111.
Fukui Y, Togawa M, Abe N, Takano Y, Asada M, Okada A, Iıda K, Ishıkawa H, Ohsumı S. (2004) Validation of sperm quality analyzer and the hypo-osmotic swelling test for frozen-thawed ram and minke whale (Balaenoptera bonarensi) spermatozoa. J. of Reprod. And Dev 50: 147-154.
García-Pérez MA, Pineda B, Hermenegildo C, Tarin JJ, Cano A. (2009) Isopropanolic Cimicifuga racemosa is favorable on bone markers but neutral on an osteoblastic cell line. Fertil Steril 91(4): 337–42.
Gil MI, Francisco A. Tomás-B, Betty Hess-P, Holcroft DM, Kader AA. (2000) Antioxidant Activity of Pomegranate Juice and Its Relationship with Phenolic Composition and Processing. J Agric Food Chem 48: 4581–9.
Guo CJ, Wei JY, Yang J, Xu J, Pang W, et al. (2008) Pomegranate juice is potentially better than apple juice in improving antioxidant function in elderly subjects. Nutrition Research 28: 72–77.
Gurib-Fakim A. (2006) Medicinal plants: Traditions of yesterday and drugs of tomorrow. Mol Aspects Med 27: 1–93.
Hancock JL. (1952) The Morphology of Bull Spermatozoa. J Exp Biol 29:445–53.
Hussen WM, Arrack JK. (2014) Protective Role of Pomegranate Peel Extract on Testis in Adult Male Rabbits Treated with Carbon Tetrachloride 38: 74–82.
Lansky EP, Harrison G, Froom P, Jiang WG. (2005) Pomegranate (Punica granatum) pure chemicals show possible synergistic inhibition of human PC-3 prostate cancer cell invasion across Matrigel??? Invest New Drugs 23: 121–2.
Leiva KP, Rubio J, Peralta F, Gonzales GF. (2011) Effect of Punica granatum (pomegranate) on sperm production in male rats treated with lead acetate. Toxicol Mech Methods 21: 495–502.
Madrigal-Carballo S, Rodriguez G, Krueger CG, Dreher M, Reed JD. (2009) Pomegranate (Punica granatum) supplements: Authenticity, antioxidant and polyphenol composition. J Funct Foods 1: 324–9.
Malik A, Mukhtar H. (2006) Prostate cancer prevention through pomegranate fruit. Cell Cycle 5: 371–3.
Mansour MM. (2009) Modification of hypo-osmotic swelling test to evaluate the integrity of stallion sperm plasma membrane. Glob. Vet 3: 302-307.
Mansour SW, Sangi S, Harsha S, Khaleel MA, Ibrahim ARN. (2013) Sensibility of male rats fertility against olive oil, Nigella sativa oil and pomegranate extract. Asian Pac J Trop Biomed 3: 563–8.
Neurath AR, Strick N, Li YY, Debnath AK. (2005) Punica granatum (pomegranate) juice provides an HIV-1 entry inhibitor and candidate topical microbicide. Ann N Y Acad Sci 1056: 311–27.
Rosenblat M, Hayek T, Aviram M. (2006) Anti-oxidative effects of pomegranate juice (PJ) consumption by diabetic patients on serum and on macrophages. Atherosclerosis 187: 363–71.
Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, et al. (2005) In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem 16: 360–7.
Seeram NP, Lee R, Heber D. (2004) Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice. Clin Chim Acta 348: 63–8.
Seeram NP, Zhang Y, Reed JD, Krueger CG, Vaya J. (2006) Pomegranates. In: Ancient Roots to Modern Medicine. Medicinal and Aromatic Plants — Industrial Profiles California, USA.
Sikka S.C. (1996) Oxıdatıve Stress and Role Of Antıoxıdants In Normal And Abnormal Sperm Functıon. Frontiers in Bioscience 1: 78-86.
Singh RP., Murthy KNC, Jayaprakasha GK. (2002) Studies on the Antioxidant Activity of Pomegranate (Punica granatum ) Peel and Seed Extracts Using in Vitro Models. J. Agric. Food Chem 50(1):81–6.
Sönmez M, Türk G, Yüce A. (2005) The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology 63: 2063–72.
Tehranifar A, Selahvarzi Y, Kharrazi M, Bakhsh VJ. (2011) High potential of agro-industrial by-products of pomegranate (Punica granatum L.) as the powerful antifungal and antioxidant substances. Ind Crops Prod 34: 1523–7.
Teixeira da Silva JA, Rana TS, Narzary D, Verma N, Meshram DT, Ranade SA. (2013) Pomegranate Biology And Biotechnology: A Review. Sci Hortic (Amsterdam) 160: 85–107.
Thring TSA, Hili P, Naughton DP. (2009) Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complement Altern Med 9(27): 1-11.
Tremellen K. (2008) Oxidative stress and male infertility — a clinical perspective 14: 243–58.
Türk G, Ateşşahin A, Sönmez M, Çeribaşi AO, Yüce A. (2008a) Improvement of cisplatin-induced injuries to sperm quality, the oxidant-antioxidant system, and the histologic structure of the rat testis by ellagic acid. Fertil Steril 89: 1474–81.
Türk G, Sönmez M, Aydin M, Yüce A, Gür S, Yüksel M, et al. (2008b) Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clin Nutr 27: 289–96.
Türk G, Sönmez M, Çeribaşi AO, Yüce A, Ateşahin A. (2010) Attenuation of cyclosporine A-induced testicular and spermatozoal damages associated with oxidative stress by ellagic acid. Int Immunopharmacol 10:177–82.
Tzulker R, Glazer I, Bar-Ilan I, Holland D, Aviram M, Amir R. (2007) Antioxidant activity, polyphenol content, and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions. J Agric Food Chem 55: 9559–70.
Yılmaz B. (2010) Therapeutic use of pomegranate (Punica granatum). Turkiye Aile Hekim Derg 14: 146–53.
Zeweil HS, Elnagar S, Zahran SM, Ahmed MH, Gindy EY. (2013)
Pomegranate peel as a natural antioxidant boosts bucks’ fertility under egyptian summer conditions. World Rabbit Sci 21: 33–9.
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