| More

Carry over effect in pesticide residues analysis by LC - MS/ MS

Views: 210 Downloads: 146
G. MILIADIS, P. MALATOU, P. TSIANTAS, A. VRETTAKOU, V. I. EVANGELOU, Ch. ANAGNOSTOPOULOS, G. SIRAGAKIS
G. MILIADIS, P. MALATOU, P. TSIANTAS, A. VRETTAKOU, V. I. EVANGELOU, Ch. ANAGNOSTOPOULOS, G. SIRAGAKIS

Abstract


The Carry over effect, i.e. the appearance of a peak in the chromatogram of a blank analysis due to sample remaining from the previous analysis, was studied. The study was conducted for pesticide residues analysis by LC-MS/MS. In total 128 pesticides that belong to 13 different chemical classes were analyzed in order to investigate the cases that the effect is significant; i.e. a peak higher than 1% of the peak of the previous chromatogram appears. Carry over was found for 32 of the 128 studied pesticides (25%), at concentration levels between the LOD and 0.167 μg/mL. For 28 out of the 32 substances, more than two injections of a blank sample were required, as to reduce the effect significantly. Compounds presenting Carry over effect were mainly non-polar with logkow values between 4 and 7, characterized with very low water solubility, between 0.001 mg/L and 2 mg/L. On the contrary, the vapor pressure did not seem to be obviously related to the effect, as the substances presenting Carry over had various vapor pressure values, from 10-12 to 0.2 mP.


Keywords


Pesticides residues; Carry over; LC-MS/MS

Full Text:

PDF

References


Anagnostopoulos C, Miliadis GE (2013) Development and validation of an easy multiresidue method for the determination of multiclass pesticide residues using GC-MS/MS and LC-MS/MS in olive oil and olives. Talanta. 112C, 1-10.

Anonymous (2013) Why do I have carry over? Chromatography today, Aug-Sep: pp 40-44.

DG-SANΤΕ (2015) Guidance Document on Analytical Quality Control and Validation Procedures for Pesticide Residues Analysis in Food and Feed. SANTE/11945/2015.

Dolan J (2001) Attacking carry over problems. LCGC 19: 1050-1054.

EC (2008) Foods of plant origin - Determination of pesticide residues using GC-MS and/or LC-MS/MS following acetonitrile extraction/partitioning and clean-up by dispersive SPE - QuEChERS-method. EN 15662:2008

Shimadzu. (2008) Technical report: HPLC Techniques to improve LC/MS performance and productivity. Vol 17.

Research article


Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 G MILIADIS, P MALATOU, P TSIANTAS, A VRETTAKOU, VI EVANGELOU, Ch. ANAGNOSTOPOULOS, G SIRAGAKIS

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.