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Carry over effect in pesticide residues analysis by LC - MS/ MS

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G. MILIADIS, P. MALATOU, P. TSIANTAS, A. VRETTAKOU, V. I. EVANGELOU, Ch. ANAGNOSTOPOULOS, G. SIRAGAKIS
G. MILIADIS, P. MALATOU, P. TSIANTAS, A. VRETTAKOU, V. I. EVANGELOU, Ch. ANAGNOSTOPOULOS, G. SIRAGAKIS

Abstract


The Carry over effect, i.e. the appearance of a peak in the chromatogram of a blank analysis due to sample remaining from the previous analysis, was studied. The study was conducted for pesticide residues analysis by LC-MS/MS. In total 128 pesticides that belong to 13 different chemical classes were analyzed in order to investigate the cases that the effect is significant; i.e. a peak higher than 1% of the peak of the previous chromatogram appears. Carry over was found for 32 of the 128 studied pesticides (25%), at concentration levels between the LOD and 0.167 μg/mL. For 28 out of the 32 substances, more than two injections of a blank sample were required, as to reduce the effect significantly. Compounds presenting Carry over effect were mainly non-polar with logkow values between 4 and 7, characterized with very low water solubility, between 0.001 mg/L and 2 mg/L. On the contrary, the vapor pressure did not seem to be obviously related to the effect, as the substances presenting Carry over had various vapor pressure values, from 10-12 to 0.2 mP.


Keywords


Pesticides residues; Carry over; LC-MS/MS

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References


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Copyright (c) 2018 G MILIADIS, P MALATOU, P TSIANTAS, A VRETTAKOU, VI EVANGELOU, Ch. ANAGNOSTOPOULOS, G SIRAGAKIS

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