L-lysine determination in animal feed using microbiological microtiter plate assay
Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such as accuracy, precision, limit of detection, and limit of determination were evaluated. Results of total L-lysine determination in different feed samples have been compared with results of validated HPLC method. The microbiological microtiter plate assay method can be employed as a qualitative and quantification method for total L-lysine determination with detection and determination limit of 0.040 % and 0.085 %, respectively. However, further research on the influence of sample matrix on the determination of low lysine levels is required.
- How to Cite
JAKŠIĆ, S., JAJIĆ, I., KRSTOVIĆ, S., & MIHALJEV, Ž. (2018). L-lysine determination in animal feed using microbiological microtiter plate assay. Journal of the Hellenic Veterinary Medical Society, 69(3), 1094–1100. https://doi.org/10.12681/jhvms.18881
- Vol. 69 No. 3 (2018)
- Research Articles
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