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Combined efficacy of silver nanoparticles and commercial antibiotics on different phylogenetic groups of Escherichia coli

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A. KAZEMNIA, M. AHMADI, K. MARDANI, M. MORADI, R. DARVISHZADEH
A. KAZEMNIA, M. AHMADI, K. MARDANI, M. MORADI, R. DARVISHZADEH

Abstract


Silver nanoparticles (Ag-NPs) can attach to flexible polymeric chains of antibiotics, hence it can be used in combination with antibiotics against resistant bacteria. In this study, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and MBC/MIC ratio of Ag-NPs and antibiotics (gentamicin, tetracycline, erythromycin, ciprofloxacin, nalidixic acid, cefixime, cephalexin, amoxicillin, ampicillin, and penicillin) were quantified against 50 Escherichia coli isolates (25 human urinary tract infection and 25 avian colibacillosis). All isolates had been assigned as four phylogenetic groups A, B1, B2, and D. The results showed that the majority of the human and broiler isolates belonged to phylogenetic groups A and B2. MBC/MIC ratio of Ag-NPs in combination with antibiotics was assessed. It was found that the MIC of the majority of broiler isolates to Ag-NPs was equal to or greater than 50 μg/ml. To conclude, a combination of penicillin and ciprofloxacin with Ag-NPs exhibited profound impact against isolates, the combinations might be applicable for treating multidrug-resistant bacteria.


Keywords


Escherichia coli; MBC/MIC ratio; silver nanoparticles; phylotypes

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Skjot-Rasmussen L, Ejrnaes K, Lundgren B, Hammerum AM, Frimodt-Moller N (2012) Virulence factors and phylogenetic grouping of Escherichia coli isolates from patients with bacteraemia of urinary tract origin relate to sex and hospital- vs. community-acquired origin. Int J Med Microbiol 302(3):129-134.

Johnson TJ, Kariyawasam S, Wannemuehler Y, Mangiamele P, Johnson SJ, et al. (2007) The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes. J Bacteriol 189(8):3228-3236.

Hussain A, Ewers C, Nandanwar N, Guenther S, Jadhav S, et al. (2012) Multiresistant uropathogenic Escherichia coli from a region in India where urinary tract infections are endemic: genotypic and phenotypic characteristics of sequence type 131 isolates of the CTX-M-15 extended-spectrum-beta-lactamase-producing lineage. Antimicrob Agents Chemother 56(12):6358-6365.

Kazemnia A, Ahmadi M, Dilmaghani M (2014) Antibiotic resistance pattern of different Escherichia coli phylogenetic groups isolated from human urinary tract infection and avian colibacillosis. Iran Biomed J 18(4):219-224.

Singer RS (2015) Urinary tract infections attributed to diverse ExPEC strains in food animals: evidence and data gaps. Front Microbiol 6:28.

Walk ST, Mladonicky JM, Middleton JA, Heidt AJ, Cunningham JR, et al. (2007) Influence of antibiotic selection on genetic composition of Escherichia coli populations from conventional and organic dairy farms. Appl Environ Microbiol 73(19):5982-5989.

Furtula V, Farrell EG, Diarrassouba F, Rempel H, Pritchard J, et al. (2010) Veterinary pharmaceuticals and antibiotic resistance of Escherichia coli isolates in poultry litter from commercial farms and controlled feeding trials. Poult sci 89(1):180-188.

Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, et al. (2007) Antimicrobial effects of silver nanoparticles. Nanomedicine 3(1):95-101.

Park EJ, Bae E, Yi J, Kim Y, Choi K, et al. (2010) Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environ Toxicol Pharmacol 30(2):162-168.

Ansari MA, Khan HM, Khan AA, Ahmad MK, Mahdi AA, et al. (2014) Interaction of silver nanoparticles with Escherichia coli and their cell envelope biomolecules. J Basic Microbiol 54(9):905-915.

You C, Han C, Wang X, Zheng Y, Li Q, et al. (2012) The progress of silver nanoparticles in the antibacterial mechanism, clinical application and cytotoxicity. Mol Biol Rep 39(9):9193-9201.

Yang E-J, Jang J, Kim S, Choi I-H (2012) Silver Nanoparticles as a Smart Antimicrobial Agent. J Bacteriol Virol 42(2):177.

Tamboli MS, Kulkarni MV, Patil RH, Gade WN, Navale SC, et al. (2012) Nanowires of silver-polyaniline nanocomposite synthesized via in situ polymerization and its novel functionality as an antibacterial agent. Colloids Surf B 92:35-41.

Silambarasan S, Jayanthi A (2013) Biosynthesis of silver nanoparticles using Pseudomonas fluorescens. Res J Biotechnol 8(3):71-74.

Xu H, Qu F, Xu H, Lai W, Andrew Wang Y, et al. (2012) Role of reactive oxygen species in the antibacterial mechanism of silver nanoparticles on Escherichia coli O157:H7. BioMetals 25(1):45-53.

Ghosh S, Patil S, Ahire M, Kitture R, Kale S, et al. (2012) Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents. Int J Nanomed 7:483-496.

Chudasama B, Vala AK, Andhariya N, Mehta RV, Upadhyay RV (2010) Highly bacterial resistant silver nanoparticles: synthesis and antibacterial activities. J Nanopart Res 12(5):1677-1685.

Clermont O, Bonacorsi S, Bingen E (2000) Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 66(10):4555-4558.

CLSI (2012) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard—Ninth Edition. Wayne, PA: Clinical and Laboratory Standards Institute.

Ayala-Núñez NV, Lara Villegas HH, del Carmen Ixtepan Turrent L, Rodríguez Padilla C (2009) Silver Nanoparticles Toxicity and Bactericidal Effect Against Methicillin-Resistant Staphylococcus aureus: Nanoscale Does Matter. NanoBiotechnology 5(1-4):2-9.

Ansari MA, Khan HM, Khan AA, Pal R, Cameotra SS (2013) Antibacterial potential of Al2O3 nanoparticles against multidrug resistance strains of Staphylococcus aureus isolated from skin exudates. J Nanopart Res 15(10):1.

Radzig MA, Nadtochenko VA, Koksharova OA, Kiwi J, Lipasova VA, et al. (2013) Antibacterial effects of silver nanoparticles on gram-negative bacteria: influence on the growth and biofilms formation, mechanisms of action. Colloids Surf B 102:300-306.

Lavanya M, Veenavardhini SV, Gim GH, Kathiravan MN, Kim SW (2013) Synthesis, Characterization and Evaluation of Antimicrobial Efficacy of Silver Nanoparticles using Paederia foetida L. leaf extract. Int res j biol 2(3):28-34.

Dhas SP, Mukherjee A, Chandrasekaran N (2013) Synergistic Effect of Biogenic Silver Nanocolloid in Combination with Antibiotics: A Potent Therapeutic Agent. Int J Pharm Pharm Sci 5:1292-295.

Prema P, Thangapandiyan S, Immanuel G (2017) CMC stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics. Carbohydr Polym 158:141-148.

Das B, Dash S, Mandal D, Adhikary J, Chattopadhyay S, et al. (2016) Greensynthesized silver nanoparticles kill virulent multidrugresistant Pseudomonas aeruginosa strains: A mechanistic study. BLDE Univ J Health Sci 1(2):89-101.

Barapatre A, Aadil KR, Jha H (2016) Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus. Bioresour Bioprocess 3(1):8-21.

Jamaran S, Zarif BR (2016) Synergistic Effect of Silver Nanoparticles with Neomycin or Gentamicin Antibiotics on Mastitis-Causing Staphylococcus aureus. Open J Ecol 06(07):452-459.

Katva S, Das S, Moti HS, Jyoti A, Kaushik S (2017) Antibacterial Synergy of Silver Nanoparticles with Gentamicin and Chloramphenicol against Enterococcus faecalis. Pharmacogn Mag 13:828-833.

Ebrahimi A, Azarban H, Habibian S, Mahzunieh M, Lotfalian S (2017) Evaluation of Anti-biofilm and Antibiotic Synergistic Activities of Silver Nanoparticles Against Some Common Bacterial Pathogens. Int J Basic Sci Med 2(3):128-132.

Panacek A, Smekalova M, Kilianova M, Prucek R, Bogdanova K, et al. (2015) Strong and Nonspecific Synergistic Antibacterial Efficiency of Antibiotics Combined with Silver Nanoparticles at Very Low Concentrations Showing No Cytotoxic Effect. Molecules 21(1):E26.

Mohamed MA, Mohamed FM, El-Said WA (2017) Enhancement of Antimicrobial Sensitivity of Salmonella and Escherichia coli Strains Isolated from Chickens Using Silver Nanoparticles in Assiut Governorate. Zagazig Vet J 45(3):273-282.

Chhibber S, Gondil VS, Sharma S, Kumar M, Wangoo N, et al. (2017) A Novel Approach for Combating Klebsiella pneumoniae Biofilm Using Histidine Functionalized Silver Nanoparticles. Front Microbiol 8:1104-1114.

Satapathy S, Kumar S, Sukhdane KS, Shukla SP (2017) Biogenic synthesis and characterization of silver nanoparticles and their effects against bloom-forming algae and synergistic effect with antibiotics against fish pathogenic bacteria. J App Phycol 29(4):1865-1875.

Wang Y-W, Tang H, Wu D, Liu D, Liu Y, et al. (2016) Enhanced bactericidal toxicity of silver nanoparticles by the antibiotic gentamicin. Environ Sci Nano 3(4):788-798.

Verma S, Abirami S, Mahalakshmi V (2017) Anticancer and antibacterial activity of silver nanoparticles biosynthesized by Penicillium spp. and its synergistic effect with antibiotics. Res Rev J Microbiol Biotechnol 3(3):18.

Natan M, Banin E (2017) From Nano to Micro: using nanotechnology to combat microorganisms and their multidrug resistance. FEMS Microbiol Rev 41(3):302-322.

Deng H, McShan D, Zhang Y, Sinha SS, Arslan Z, et al. (2016) Mechanistic Study of the Synergistic Antibacterial Activity of Combined Silver Nanoparticles and Common Antibiotics. Environ Sci Technol 50(16):8840-8848.

Yallappa S, Manjanna J, Dhananjaya BL (2015) Phytosynthesis of stable Au, Ag and Au-Ag alloy nanoparticles using J. sambac leaves extract, and their enhanced antimicrobial activity in presence of organic antimicrobials. Spectrochim Acta A Mol Biomol Spectrosc 137:236-243.

Refat MS, Sharshar T, Elsabawy KM, El-Sayed MY, Adam AMA (2017) Synthesis of new drug model has an effective antimicrobial and antitumors by combination of cephalosporin antibiotic drug with silver(I) ion in nano scale range: Chemical, physical and biological studies. J Mol Liq 244:169-181.

Tawfeeq SM, Maaroof MN, Al-Ogaidi I (2017) Synergistic effect of biosynthesized silver nanoparticles with antibiotics against multi-drug resistance bacteria isolated from children with diarrhoea under five years. Iraqi J Sci 58(1):41-52.

Zheng B, Setyawati MI, Leong DT, Xie J (2018) Antimicrobial silver nanomaterials. Coord Chem Rev 357:1-17.

Majeed S, Abdullah MSb, Dash GK, Ansari MT, Nanda A (2016) Biochemical synthesis of silver nanoprticles using filamentous fungi Penicillium decumbens (MTCC-2494) and its efficacy against A-549 lung cancer cell line. Chin J Nat Med 14(8):615-620.

Nagy A, Harrison A, Sabbani S, Munson RS, Jr., Dutta PK, et al. (2011) Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action. Int J Nanomedicine 6:1833-1852.

Silver S, Phung le T, Silver G (2006) Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds. J Ind Microbiol Biotechnol 33(7):627-634.

Muhling M, Bradford A, Readman JW, Somerfield PJ, Handy RD (2009) An investigation into the effects of silver nanoparticles on antibiotic resistance of naturally occurring bacteria in an estuarine sediment. Mar Environ Res 68(5):278-283.


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Copyright (c) 2019 A. KAZEMNIA, M. AHMADI, K. MARDANI, M. MORADI, R. DARVISHZADEH

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