Combined efficacy of silver nanoparticles and commercial antibiotics on different phylogenetic groups of Escherichia coli

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DOI: https://doi.org/10.12681/jhvms.21788
Keywords:
Escherichia coli MBC/MIC ratio silver nanoparticles phylotypes
A. KAZEMNIA
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
M. AHMADI
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
K. MARDANI
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
M. MORADI
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
R. DARVISHZADEH
Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran
Abstract

Silver nanoparticles (Ag-NPs) can attach to flexible polymeric chains of antibiotics, hence it can be used in combination with antibiotics against resistant bacteria. In this study, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and MBC/MIC ratio of Ag-NPs and antibiotics (gentamicin, tetracycline, erythromycin, ciprofloxacin, nalidixic acid, cefixime, cephalexin, amoxicillin, ampicillin, and penicillin) were quantified against 50 Escherichia coli isolates (25 human urinary tract infection and 25 avian colibacillosis). All isolates had been assigned as four phylogenetic groups A, B1, B2, and D. The results showed that the majority of the human and broiler isolates belonged to phylogenetic groups A and B2. MBC/MIC ratio of Ag-NPs in combination with antibiotics was assessed. It was found that the MIC of the majority of broiler isolates to Ag-NPs was equal to or greater than 50 μg/ml. To conclude, a combination of penicillin and ciprofloxacin with Ag-NPs exhibited profound impact against isolates, the combinations might be applicable for treating multidrug-resistant bacteria.

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