Distribution of serotypes of Listeria monocytogenes in chicken meats in Turkey

Listeria monocytogenes food-borne pathogen serotype 1/2a mPCR chicken meats

Listeria monocytogenes is one of the important causes of food-borne infections. This study was conducted to determine the presence of L. monocytogenes and its serotype distribution in a total of 400 packaged chicken meat products (drumstick, breast, wing, and whole chicken) from different national companies. L. monocytogenes contamination was detected in 26.5% (106 in 400) of all samples when the products considered, drumsticks, breasts, wings, and whole chickens showed 47%, 15%, 35, and 9% positivity respectively. Four important serotypes of L. monocytogenes in human listeriosis (1/2a, 1/2b, 1/2c and 4b) were identified, and serotype 1/2a (94.3%) was determined as predominant in packaged chicken meats. The present study revealed that L. monocytogenes 1/2a serotype is prevalent in chicken meats and this may cause public health problems in Turkey. Further studies in poultry meats should be conducted on a large scale such as regional or national big markets to determine the presence of the pathogen and its dominant serotypes.

Article Details
  • Section
  • Research Articles
Download data is not yet available.
Angelidis AS, Kalamaki MS, Georgiadou SS (2015) Identification of non-Listeria spp. bacterial isolates yielding a β-D-glucosidase-positive phenotype on Agar Listeria according to Ottaviani and Agosti (ALOA). Int J Food Microbiol 193:114-129.
Ayaz ND, Erol I (2009) Rapid detection of Listeria monocytogenes in ground turkey by immunomagnetic separation and PCR. J Rapid Met Aut Microbiol 17:214-222.
Centers for Disease Control and Prevention (CDC) (2014) https://www.cdc.gov/listeria/pdf/listeriainitiativeoverview_508.pdf. [accessed 17
August 2016]
Centers for Disease Control and Prevention (CDC) (2016) https://www.cdc.gov/foodborneburden/2011-foodborne-estimates.html. [accessed
November 2016]
Cetinkaya F, Elal Mus T, Yibar A, Guclu N,Tavsanli H, Cibik R (2014) Prevalence, serotype identification by multiplex polymerase chain reaction and antimicrobial resistance patterns of Listeria monocytogenes isolated from retail foods. J Food Safety 34:42-49.
Churchill KJ, Sargeant JM, Farber JM, O’Connor AM (2019) Prevalence of Listeria monocytogenes in select ready-to-eat foods-deli meat, soft cheese, and packaged salad: A systematic review and meta-analysis. J Food Prot 82(2):344-357.
D’Agostino M, Wagner M, Vazquez-Boland JA, Kuchta T, Karpıskova R, Hoorfar J, Novella S, Scortti M, Ellison J, Murray A, Fernandes I, Kuhn M, Pazlarova J, Heuveliink A, Cook N (2004) A validated PCR-based method to detect Listeria monocytogenes using raw milk as a food model-towards an international standard. J Food Prot 67:1646-1655.
Dhama K, Karthik K, Tiwari R, Shabbir MZ, Barbuddhe S, Malik SVS, Singh RK (2015) Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review. Vet Q 35:211-235.
Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P (2004) Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 42:3819-3822.
Elmali M, Can HY, Yaman H (2015) Prevalence of Listeria monocytogenes in poultry meat. Food Sci Technol (Campinas) 35:672-675.
Erol İ, Şireli UT, Gündeş B (1999) Piliç parça et ve iç organlarında Listeria türlerinin varlığı ve kontaminasyon düzeyinin belirlenmesi. Ankara Üniv Vet Fak Derg 46:179-188.
European Food Safety Authority (EFSA) (2010) https://www.efsa.europa. eu/en/efsajournal/pub/1496. [accessed 20 May 2017]
European Food Safety Authority (EFSA) (2015) https://www.efsa.europa.eu/en/efsajournal/pub/4329. [accessed 16 July 16 2017]
European Food Safety Authority (EFSA) (2018) https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2018.5500. [accessed 25.06.2019]
Fallah AA, Saei-Dehkordi SS, Rahnama M, Tahmasby H, Mahzounieh MR (2012) Prevalence and antimicrobial resistance patterns of Listeria species isolated from poultry products marketed in Iran. Food Control 28:327-332.
Farber JM, Peterkin PI (1991) Listeria monocytogenes, a food borne pathogen. Microbiol Rev 55:476-511.
Goh SG, Kuan CH, Loo YY, Chang WS, Lye YL, Soopna P, Tang JY, Nakaguchi Y, Nishibuchi M, Afsah-Hejri L, Son R (2012) Listeria monocytogenes in retailed raw chicken meat in Malaysia. Poultry Sci 91:2686-2690.
Guerra MM, McLauchin J, Bernardo FA (2001) Listeria in ready-to-eat and unprocessed foods produced in Portugal. Food Microbiol 18:423-429.
Guven A. Patir B (1998) Elazığ ilinde tüketime sunulan et ve bazı et ürünlerinde Listeria türlerinin araştırılması. Turk J Vet Anim Sci 22:205-212.
Jamali H, Radmehr B, Thong KL (2013) Prevalence, characterisation, and antimicrobial resistance of Listeria species and Listeria monocytogenes isolates from raw milk in farm bulk tanks. Food Control 34:121-125.
Jeyaletchumi P, Tunung R, Margaret SP, Son R, Farinazleen MG, Cheah YK (2010) Detection of Listeria monocytogenes in foods. Int Food Res J 17:1-11.
Karthikeyan R, Gunasekaran P, Rajendhran J (2015) Molecular serotyping and pathogenic potential of Listeria monocytogenes isolated from milk and milk products in Tamil Nadu, India. Foodborne Pathog Dis 12:522-528.
Kurpas M, Wieczorek K, Osek J (2018) Ready-to-eat meat products as a source of Listeria monocytogenes. J Vet Res 62(1):49-55.
Liu D (2013) Molecular approaches to the identification of pathogenic and nonpathogenic listeriae. Microbiol Insights 6:59-69.
Lomonaco S, Nucera D, Filipello V (2015) The evolution and epidemiology of Listeria monocytogenes in Europe and the United States. Infect Genet Evol 35:172-183.
López V, Navas J, Martínez-Suárez JV (2013) Low potential virulence associated with mutations in the inlA and prfA genes in Listeria monocytogenes isolated from raw retail poultry meat. J Food Prot 76:129-132.
Nho SW, Abdelhamed H, Reddy S, Karsi A, Lawrence ML (2015) Identification of high-risk Listeria monocytogenes serotypes in lineage I (serotype 1/2a, 1/2c, 3a and 3c) using multiplex PCR. J Appl Microbiol 119:845-852.
Oliveira TS, Varjão LM, da Silva LNN, Pereira RDCL, Hofer E, Vallim DC, de Castro Almeida RC (2018) Listeria monocytogenes at chicken slaughterhouse: Occurrence, genetic relationship among isolates and evaluation of antimicrobial susceptibility. Food Control 88:131-138.
Orsi RH, Bakker HC, Wiedmann M (2011) Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. Int J Med Microbiol 301:79-96.
Praakle-Amin A, Hänninen ML, Korkeala H (2006) Prevalence and genetic characterization of Listeria monocytogenes in retail broiler meat in Estonia. J Food Prot 69:436-440.
Rocourt J, Buchriser C (2007) The Genus Listeria and Listeria monocytogenes: Phylogenetic Position, Taxonomy and Identification. In: Listeria Listeriosis and Food Safety. Marcel Dekker, New York: pp 1-20.
Rothrock MJ, Davis ML, Locatelli A, Bodie A, Mcintosh TG, Donaldson JR, Ricke SC (2017) Listeria occurrence in poultry flocks: detection and potential implications. Front Vet Sci 4:125.
Rothrock MJ Jr, Micciche AC, Bodie AR, Ricke SC (2019) Listeria occurrence and potential control strategies in alternative and conventional poultry processing and retail. Front Sustain Food Syst 3:33.
Siriken B, Ayaz ND, Erol I (2014) Listeria monocytogenes in retailed raw chicken meat in Turkey. Berl Munch Tierarztl Wochenschr 127:43-49.
Soultos N, Koidis P, Madden RH (2003) Presence of Listeria and Salmonella spp. in retail chicken in Northern Ireland. Lett Appl Microbiol 37:421-423.
Todd ECD Notermans S (2011) Surveillance of listeriosis and its causative pathogen, Listeria monocytogenes. Food Control 22:1484-1490.
Turkish Poultry Meat Producers and Breeders Association (TPMPBA) (2018) http://www.besdbir.org/assets/documents/sectorInformation/Turkey_Poultry_Meat_Production.pdf [accessed 30 December 2018]
Vardar İ, Yurtsever GS, Coşkun AN, Kaptan F, El S, Ural S (2011) Listeria monocytogenes’e bağlı bir menenjit olgusu. ANKEM Derg 25:54-57.
Zhang Y, Yeh E, Hall G, Cripe J, Bhagwat AA. Meng J (2007) Characterization of Listeria monocytogenes isolated from retail foods. Int J Food Microbiol 113:47-53.
Most read articles by the same author(s)