Creation and Characterization of the β-1,4-Glucanase (CMCase)Producing Mutant Strains of Bacillus sp. with Ultraviolet Radiation and Ethidium Bromide

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Published: Apr 18, 2023
Updated: 2023-04-18
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2023-04-18 (2)
DOI: https://doi.org/10.12681/jhvms.24694
Keywords:
CMCase β-1 4-glucanase mutation ethidium bromide UV radiation Bacillus sp.
A Sezan
Çukurova University Institute of Science
https://orcid.org/0000-0003-1506-7083
M Baylan
2Cukurova University, Faculty of Fisheries,
https://orcid.org/0000-0003-0549-0662
Abstract

In this study, the β-1,4-glucanase-producing Bacillus sp. strains were isolated from soil; then, in these strains, the undesired enzyme genes such as α-amylase, xylanase, protease were inactivated by mutation using Ethidium bromide (EtBr) and UV ray; thus, only β-1,4-glucanase producing strains were obtained.  The enzyme activities (pH, temperature, thermostability, etc.) of the wild strain and its two mutant variants (UV mutant (mUV), EtBr mutant (mEB)) producing β-1,4-glucanase were partially characterized. It was found that the optimum pHs for the mUV, mEB, and the wild strain were 7.0, 6.0 and 9.0, respectively; and the optimum temperatures were 50, 60, and 50 ℃, respectively. Finally, the molecular weights of the cellulase enzymes of all the three bacteria were found to be 40 kDa by SDS-PAGE and zymogram analysis.

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Author Biographies
A Sezan, Çukurova University Institute of Science

Department of Biotechnology

M Baylan, 2Cukurova University, Faculty of Fisheries,

Department of Basic Science

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