Creation and Characterization of the β-1,4-Glucanase (CMCase)Producing Mutant Strains of Bacillus sp. with Ultraviolet Radiation and Ethidium Bromide
Abstract
In this study, the β-1,4-glucanase-producing Bacillus sp. strains were isolated from soil; then, in these strains, the undesired enzyme genes such as α-amylase, xylanase, protease were inactivated by mutation using Ethidium bromide (EtBr) and UV ray; thus, only β-1,4-glucanase producing strains were obtained. The enzyme activities (pH, temperature, thermostability, etc.) of the wild strain and its two mutant variants (UV mutant (mUV), EtBr mutant (mEB)) producing β-1,4-glucanase were partially characterized. It was found that the optimum pHs for the mUV, mEB, and the wild strain were 7.0, 6.0 and 9.0, respectively; and the optimum temperatures were 50, 60, and 50 ℃, respectively. Finally, the molecular weights of the cellulase enzymes of all the three bacteria were found to be 40 kDa by SDS-PAGE and zymogram analysis.
Article Details
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Sezan, A., & Baylan, M. (2023). Creation and Characterization of the β-1,4-Glucanase (CMCase)Producing Mutant Strains of Bacillus sp. with Ultraviolet Radiation and Ethidium Bromide. Journal of the Hellenic Veterinary Medical Society, 74(1), 5119–5130. https://doi.org/10.12681/jhvms.24694
- Issue
- Vol. 74 No. 1 (2023)
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- Research Articles
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