Effect of trans-resveratrol or ubiquinol supplementation on the sperm morphology of CD-1 mice subjected to forced swimming
Keywords:
swimming exercise sperm morphology ubiquinol trans-resveratrol
Abstract
This study was undertaken to assess the effect of a three-minute forced swimming protocol for 50 days, with and without antioxidant administration on sperm morphology in CD-1 mice. Seventy-five mice were randomly allocated to one of the following five groups: no exercise (control group; CG), swimming without antioxidant administration (EX), swimming with trans-resveratrol administration (EX-Resv), swimming with ubiquinol and excipient administration (Kaneka´s ubiquinol) (EX-Ubiq), and swimming with just only the excipient for Kaneka´s ubiquinol administration (EX-Excp). The EX group showed that 53.03±4.83% of sperm had abnormal morphology, with significant differences with regards to CG (46.47±10.57%) (p<0.05). The number of sperm with abnormal morphology decreased in all groups treated with either antioxidants or with excipient; this was most noticeable in EX-Ubiq (p<0.05). The percentage of midpiece and tail, as well as multiple anomalies were greater in EX than in CG (p<0.05). While both antioxidants, as well as the excipient, decreased midpiece and head anomalies, only trans-resveratrol and ubiquinol had an effect on multiple anomalies. Furthermore, only trans-resveratrol had an effect upon tail anomalies. The imposed exercise caused alterations in CD-1 mice sperm morphology, and antioxidant treatment seems suitable to decrease morphological anomalies. Both trans- resveratrol and ubiquinol were effective in decreasing simple as well as multiple sperm anomalies.
Article Details
- How to Cite
-
VAAMONDE Δ., SÁNCHEZ DE MEDINA, A., CORTÉS Β., DÍAZ Α., & RODRÍGUEZ Ι. (2020). Effect of trans-resveratrol or ubiquinol supplementation on the sperm morphology of CD-1 mice subjected to forced swimming. Journal of the Hellenic Veterinary Medical Society, 71(3), 2349–2356. https://doi.org/10.12681/jhvms.25096
- Issue
- Vol. 71 No. 3 (2020)
- Section
- Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
Downloads
Download data is not yet available.
References
Agarwal A, Nallella KP, Allamaneni SS, Said TM (2004) Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 8:616-627.
Agarwal A, Makker K, Sharma R (2008) Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Inmunol 59:2-11.
Ahmadi S, Bashiri R, Ghadiri-Anari A, Nadjarzadeh A (2016) Antioxidant supplements and semen parameters: An evidence-based review. Int J Reprod Biomed (Yazd) 14:729-736.
Aitken RJ (1997) Molecular mechanisms regulating human sperm function. Mol Hum Reprod 3:169-173.
Aitken RJ, Baker MA (2006) Oxidative stress, sperm survival and fertility control. Mol Cell Endocrinol 250:66-69.
Aitken RJ, Roman SD (2008) Antioxidant systems and oxidative stress in the testes. Oxy Med Cell Long 1:15-24.
Akmal M, Qadri JQ, Noori S, Shahiya T, Afrozul H, Khelod, Y (2006) Improvement in human semen quality after oral supplementation of vitamin C. J Med Food 9:440-442.
Alvarez JG, Storey BT (1995) Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 42:334-346.
Arce JC, De Souza MJ, Pescatello LS, Luciano AA (1993) Subclinical alterations in hormone and semen profile in athletes. Fertil Steril 59:398-404.
Aziz N, Saleh RA, Sharma RK, Lewis-Jones I, Esfandiari N, Thomas AJ, et al (2004) Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertil Steril 81:349-354.
Balercia G, Buldreghini E, Vignini A, Tiano L, Paggi F, Amoroso S, et al (2009) Coenzyme Q10 treatment in infertile men with idiopathic asthenozoospermia: a placebo-controlled, double-blind randomized trial. Fertil Steril 91:1785-1792.
Bitgul G, Tekmen I, Keles D, Oktay G (2013) Protective effects of resveratrol against chronic immobilization stress on testis. ISRN Urology 1-10.
Brugh VM, Lipshultz LI (2004) Male factor infertility: evaluation and management. Med Clin North Am 88:367-385.
Cakiroglu B, Eyyupoglu SE, Gozukucuk R, Uyanik BS (2014) Ubiquinol Effect on Sperm Parameters in Subfertile Men Who Have Astheno-Teratozoospermia with Normal Sperm Concentration. Nephro Urol Mon 10 e16870.
Collodel G, Federico MG, Geminiani M, Martini S, Bonechi C, Rossi C, Figura N, Moretti E (2011) Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reprod Toxicol 31:239-346.
Crane FL (2001) Biochemical functions of coenzyme Q10. J Am Coll Nutr 20:591-598.
De Souza MJ, Arce JC, Pescateello LS, Scherzer HS, Luciano AA (1994) Gonadal hormones and semen quality in male runners a volume threshold effect of endurance training. Int J Sport Med 15:383-391.
De Souza MJ, Miller BE (1997) The effect of endurance training on reproductive function in male runners. A ‘volume threshold’ hypothesis. Sport Med 23:357-374.
du Plessis SS, Kashou A, Vaamonde D, Agarwal A (2011) Is There a Link between Exercise and Male Factor Infertility? Open Reprod Sci J 3:105-113.
Faul F, Erdfelder E, Buchner A, Lang AG (2009) Statistical power analyses using G*Power 3.1: Tests for correlation and regression analyses. Behav Res Methods 41: 1149-1160.
Fouad AA, Al-Sultan AI, Yacoubi M (2011) Coenzyme Q10 counteracts testicular injury induced by sodium arsenite in rats. Eur J Pharmacol 655:91-98.
Gatimel N, Moreau J, Parinaud J, Leandri R (2017) Sperm morphology: assessment, pathophysiology, clinical relevance, and state of the art in 2017. Andrology 5:845-862.
Ghanbarzadeh S, Garjani A, Ziaee M, Khorrami A (2014) Effects of L-carnitine and coenzyme Q10 on impaired spermatogenesis caused by isoproterenol in male rats. Drug Res 64:449-453.
Gibellini L, Bianchini E, De Biasi S, Nasi M, Cossarizza A, Pinti M (2015) Natural Compounds Modulating Mitochondrial Functions. Evid Based Complement Alternat Med 527209.
Gozalvez J, Tvurda E, Agarwal A (2017) Free radical and superoxide reactivity detection in semen quality assessment: past, present, and future. J Assist Reprod Genetics 34:697-707.
Griveau JF, Le Lannou D (1997) Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Andrology 20:61-69.
Ikematsu H, Nakamura K, Harashima S, Fujii K, Fukotomi N (2006) Safety assessment of coenzyme Q10 (Kaneka Q10) in healthy subjects: A double-blind, randomized, placebo-controlled trial. Reg Tox Pharm 44:212-218.
Jacob RA, Pianalto FS, Agee RE (1992) Cellular ascorbate depletion in healthy men. J Nutr 122:1111-1118.
Jana K, Samanta PK, Manna I, Ghosh P, Singh N, Khetan RP, Ray BR (2008) Protective effect of sodium selenite and zinc sulfate on intensive swimming-induced testicular gametogenic and steroidogenic disorders in mature male rats. Appl Physiol Nutr Metab 33:903-914.
Jana K, Dutta A, Chakraborty P, Manna I, Firdaus SB, Bandyopadhyay D, Chattopadhyay R, Chakravarty B (2014) Alphalipoic acid and N-acetylcysteine protects intensive swimming exercise mediated germ-cell depletion, pro-oxidant generation, and alteration of steroidogenesis in rat testis. Mol Reprod Dev 81:833-850.
Jiang Y, Peng T, Luo Y, Li MC, Lin, YH (2008) Resveratrol reestablishes spermatogenesis after testicular injury in rats caused by 2.5-hexanedione. Chin Med J 121:1204-1209.
Jouannet P, Ducot B, Feneux D, Spira A (1988) Male factors and the likelihood of pregnancy in infertile couples. I. Study of sperm characteristics. Int J Androl 11(5):379-394.
Juan ME, Gonzalez-Pons E, Munuera T, Ballester J, Rodriguez JE, Planas JM (2005) Trans-Resveratrol, a natural antioxidant from grapes, increases sperm output in healthy rats. J Nutr 135:757-760.
Li SJ, Su WD, Qiu LJ, Wang X, Liu J (2018) Resveratrol protects human sperm against cryopreservation-induced injury. Zhonghua Nan Ke Xue 24:499-503.
Litarru GP (2007) Bioenergetic and Antioxidant Properties of Coenzyme Q10: Recent Developments. Mol Biotechnology 37:31-37.
López-Dominguez JA, Khaiwesh H, González-Reyes JA, López- Lluch G, Navas P, Ramsey JJ, de Cabo R, Burón MI, Villalba, JM (2013) Dietary fat modifies mitochondrial and plasma membrane apoptotic signaling in skeletal muscle of calorie-restricted mice. Age 35:2017-2044.
Manna I, Jana K, Samanta PK (2003) Effect of intensive exercise-induced testicular gametogenic and steroidogenic disorders in mature male Wistar strain rats: a correlative approach to oxidative stress. Acta Physiol Scand 178:33-40.
Manna I, Jana K, Samantha PK (2004) Effect of different intensities of swimming exercise on testicular oxidative stress and reproductive dysfunction in mature male albino Wistar rats. Indian J Exp Biol 42:816-822.
Mendes TB, Paccola CC, de Oliveira Neves FM, Simas JN, da Costa Vaz A, Cabral RE, Vendramini V, and Miraglia SM (2016) Resveratrol improves reproductive parameters of adult rats varicocelized in peripuberty. Reproduction 152:23-35.
Menkveld R, Holleboom CA, Rhemrev JP (2011) Measurement and significance of sperm morphology. Asian J Androl 13:59-68.
Minaii B, Moayeri A, Shokri S, Habibi Roudkenar M, Golmohammadi T, Malek F, Barbarestani M (2014) Melatonin improves the sperm quality in forced swimming test induced oxidative stress in nandrolone treated Wistar rats. Acta Med Iran 52:496-504.
Mingoti GZ, Pereira RN, Monteiro CM (2003) Fertility of male adult rats submitted to forced swimming stress. Braz J Med Biol Res 36:677-681.
Moayeri A, Mokhtari T, Hedayatpour A, Abbaszadeh HA, Mohammadpou S, Ramezanikhah H, Shokri S (2017) Impact of melatonin supplementation in the rat spermatogenesis subjected to forced swimming exercise. Andrologia 18:1-9.
Nadjarzadeh A, Shidfar F, Amirjannati N, Vafa MR, Montevalian SA, Gohari MR, Nazeri SA, Akhondi MM, Sadeghi MR (2013) Effect of Coenzyme Q10 supplementation on antioxidant enzymes activity and oxidative stress of seminal plasma: a double-blind randomised clinical trial. Andrologia 46:177-183.
Nirupama M, Devaki M, Nirupana R, Yajurvedi HN (2013) Chronic intermittent stress-induced alterations in the spermatogenesis and antioxidant status of the testis are irreversible in albino rat. J Physiol Biochem 69:58-68.
Ognjanovic BI, Markovic SD, Dorđevic NZ, Trbojevic IS, Andras AS, Saicic ZS: Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes (2010) Protective role of coenzyme Q10 and Vitamin E. Reprod Toxicol 29:191-197.
Okon UA, Utuk II (2016) Ascorbic acid treatment elevates follicle stimulating hormone and testosterone plasma levels and enhances sperm quality in albino Wistar rats. Nig Med J 57:31-36.
Olas B, Wachowicz B (2005) Resveratrol, a phenolic antioxidant with effects on blood platelet functions. Platelets 16:251-260.
Ombelet W, Menkveld R, Kruger TF, Steeno O (1995) Sperm morphology assessment: historical review in relation to fertility. Hum Reprod Update 1:543-557.
Ozaki A, Muromachi A, Sumi M, Sakai Y, Morishita K, Okamoto T (2010) Emulsification of Coenzyme Q10 using gum Arabic increase bioavailability in rats and human and improves food-processing suitability. J Nutr Sci Vitaminol 56:41-47.
Price NL, Gomes AP, Ling AJ, Duarte FV, Martin-Montalvo A, North BJ, et al (2012) SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. Cell Metab 15:675-690.
Rodriguez-Bernaldo A, Lage-Yusty MA, Lopez-Hernandez J (2009) HPLC-analysis of polyphenolic compounds in Spanish white wines and determination of their antioxidant activity by radical scavenging assay. Food Res Int 42:1018-1022.
Rodriguez I, Diaz A, Vaamonde D (2016) Assessment of the effect of prolonged forced swimming on CD-1 mice sperm morphology with and without antioxidant supplementation. Andrologia 48:277-281.
Sabeti P, Pourmasumi S, Rahiminia T, Akyash F, Talebi AR (2016) Etiologies of sperm oxidative stress. Int J Reprod Biomed 14:231-240.
Safarinejad MR (2009) Efficacy of coenzyme Q10 on semen parameters, sperm function and reproductive hormones in infertile men. J Urol 182:237-248.
Safarinejad MR, Safararinejad S, Shafiei N, Safarinejad S (2012) Effects of reduced form of Coenzyme Q10 (Ubiquinol) on semen parameters in men with idiopathic infertility a double-blind, placebo controlled, randomized study. J Urol 188:526-531.
Saki G, Rahim F, Alizadeh K (2009) Effect of forced swimming stress on count, motility and fertilization capacity of the sperm in adult rats. J Hum Reprod Sci 2:72-75.
Saki G, Rahim F, Kaysi OA (2010) Effect of forced swimming stress on In-vivo fertilization capacity of rat and subsequent offspring quality. J Hum Reprod Sci 3:32-34.
Samanta PK, Manna I, Jana K (2006) Effect of L- ascorbic acid supplementation on testicular oxidative stress and endocrine disorders in mature male rats exposed to intensive swimming exercise. Reprod Med Biol 5:145-153.
Sanghishetti V, Ghongane BB, Nayak BB (2014) Effect of vitamin C on male fertility in rats subjected to forced swimming stress. J Clin Diagn Res 8:HC05-HC08.
Slama R, Eustache F, Ducot B, Jensen TK, Jørgensen N, Horte A, Irvine S, Suominen J, Andersen AG, Auger J, Vierula M, Toppari J, Andersen AN, Keiding N, Skakkebaek NE, Spira A, Jouannet P (2002) Time to pregnancy and semen parameters: a cross-sectional study among fertile couples from four European cities. Hum Reprod 17:503-515.
Sönmez M, Türk G, Yüce A (2005) The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology 63:2063-2072.
Tremellen K (2008) Oxidative stress and male infertility-a clinical perspective. Hum Reprod Update 14:243-258.
Vaamonde D, Da Silva ME, Poblador MS, Lancho, JL (2006) Reproductive profile of physically active men after exhaustive endurance exercise. Int J Sport Med 27:680- 689.
Vaamonde D, Da Silva- Grigoletto ME, Garcia- Manso JM, Vaamonde- Lemos R, Swanson RJ, Oehninger SC (2009) Response of semen parameters to three training modalities. Fertil Steril 92:1941-1946.
Wyrobek AJ, Bruce, WR (1975) Chemical induction of sperm abnormalities in mice. Proc Natl Acad Sci USA 72:4425-4429.
