Effect of trans-resveratrol or ubiquinol supplementation on the sperm morphology of CD-1 mice subjected to forced swimming

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DOI : https://doi.org/10.12681/jhvms.25096
Δ. VAAMONDE
Morphological Sciences Department, School of Medicine and Nursing, Universidad de Córdoba, Spain
A. SÁNCHEZ DE MEDINA
Animal Surgery and Medicine Department, School of Veterinary Medicine, Universidad de Córdoba, Spain
Β. CORTÉS
Animal Surgery and Medicine Department, School of Veterinary Medicine, Universidad de Córdoba, Spain
Α. DÍAZ
Animal Surgery and Medicine Department, School of Veterinary Medicine, Universidad de Córdoba, Spain
Ι. RODRÍGUEZ
Animal Surgery and Medicine Department, School of Veterinary Medicine, Universidad de Córdoba, Spain
Résumé

This study was undertaken to assess the effect of a three-minute forced swimming protocol for 50 days, with and without antioxidant administration on sperm morphology in CD-1 mice. Seventy-five mice were randomly allocated to one of the following five groups: no exercise (control group; CG), swimming without antioxidant administration (EX), swimming with trans-resveratrol administration (EX-Resv), swimming with ubiquinol and excipient administration (Kaneka´s ubiquinol) (EX-Ubiq), and swimming with just only the excipient for Kaneka´s ubiquinol administration (EX-Excp). The EX group showed that 53.03±4.83% of sperm had abnormal morphology, with significant differences with regards to CG (46.47±10.57%) (p<0.05). The number of sperm with abnormal morphology decreased in all groups treated with either antioxidants or with excipient; this was most noticeable in EX-Ubiq (p<0.05). The percentage of midpiece and tail, as well as multiple anomalies were greater in EX than in CG (p<0.05). While both antioxidants, as well as the excipient, decreased midpiece and head anomalies, only trans-resveratrol and ubiquinol had an effect on multiple anomalies. Furthermore, only trans-resveratrol had an effect upon tail anomalies. The imposed exercise caused alterations in CD-1 mice sperm morphology, and antioxidant treatment seems suitable to decrease morphological anomalies. Both trans- resveratrol and ubiquinol were effective in decreasing simple as well as multiple sperm anomalies.

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