The effect of bromhexine on mucosal immune response against avian coronavirus infectious bronchitis vaccine in chickens

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Published: Jan 19, 2024
Updated: 2024-01-19
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2024-01-19 (4)
DOI: https://doi.org/10.12681/jhvms.25477
Keywords:
Bromhexine Mucosal immunity Infectious Bronchitis Broiler chicken
M Gholami-Ahangaran
Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
https://orcid.org/0000-0002-2725-1091
A Hjazi
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia
HA Hussny
College of Pharmacy, National University of Science and Technology, DhiQar, Iraq
AA Amir
Department of Medical Laboratories Technology, AL-Nisour University College, Baghdad, Iraq
AA Abdulhussien Alazbjee
College of Medicine, Al-Ayen University, Thi-Qar, Iraq
M Karimi-Dehkordi
Department of Clinical Pathology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract

Avian coronavirus infectious bronchitis (IB) is one of the most important and contagious disease in chickens, all over the world. Using of chemical compounds that improve the mucosal immune response to IB vaccine can increase resistance against the virus. To examine the effect of Bromhexine on the mucosal immune response against IB vaccine, 360 one-day broiler chicks were allocated to eight groups, randomly. Group one was the control group with no vaccine so that the group only used Bromhexine. Groups 2, 3, and 4 received IB vaccine for once, twice, and three times at the age of 5, 15, and 25 days, respectively. These groups received Bromhexine from 48 hrs before the vaccination to 24 hrs after receiving the vaccine in drinking water. Group five was the negative control group and did not receive Bromhexine and IB vaccine. Sampling in group five was at the age of 15 days. Groups six, seven, and eight were the main treatment control groups and received IB vaccine at the age of 5, 15, and 25 days without Bromhexine. The chickens were slaughtered 10 days after the vaccination and nose and trachea samples were collected and the mucus surface of respiratory was rinsed. Afterwards, specific immunoglobin A (IgA) level against IB vaccine in the respiratory mucus was measured through ELISA using chicken IgA specific antiglobulin. The results showed that the use of Bromhexine on the first vaccination had no effect on mucosal immune response. However, with the second and third vaccinations, antibody titter to IB vaccine was higher than chickens received vaccine without Bromhexin. It appears that using Bromhexine in booster IB vaccination can improve specific mucosal immune response.

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References
Amiri M, Gholami-Ahangaran M, & Jafarian-Dehkordi M, (2017) Modulating effect of mycoad® on performance, mucosal and systemic immunity in chickenaflatoxicosis. Slov Vet Res. 54(1), 29-35.
Carrillo L, Sumano H, Medina-Torres L, Tapia G, & Gutiérrez L (2016) Rheological study of healthy chicken's pooled tracheobronchial secretions and its modification by mucolytics drugs. Poult Sci 95(11), 2667-2672.
Gelb J, Jr., Nix WA, & Gellman SD (1998) Infectious bronchitis virus antibodies in tears and their relationship to immunity. Avian Dis 42(2), 364-374.
Gillette KG (1981) Local antibody response in avian infectious bronchitis: virus-neutralizing antibody in tracheobronchial secretions. Avian Dis 25(2), 431-443.
Gurjar RS, Gulley SL, & van Ginkel FW (2013) Cell-mediated immune responses in the head-associated lymphoid tissues induced to a live attenuated avian coronavirus vaccine. Dev Comp Immunol 41(4), 715-722.
Jackwood MW, & de Wit S (2020) Infectious bronchitis. Diseases of poultry, 167-188.
Legnardi M, Tucciarone CM, Franzo G, & Cecchinato M (2020) Infectious Bronchitis Virus Evolution, Diagnosis and Control. Vet Sci 7(2):100-110.
Maslak DM, & Reynolds DL (1995) B cells and T-lymphocyte subsets of the head-associated lymphoid tissues of the chicken. Avian Dis 39(4), 736-742.
Okino CH, Alessi AC, Montassier Mde F, Rosa AJ, Wang X, & Montassier HJ (2013) Humoral and cell-mediated immune responses to different doses of attenuated vaccine against avian infectious bronchitis virus. Viral Immunol 26(4), 259-267.
Orr-Burks N, Gulley SL, Toro H, & van Ginkel FW (2014) Immunoglobulin A as an early humoral responder after mucosal avian coronavirus vaccination. Avian Dis 58(2), 279-286.
Porter P (1973) Intestinal defence in the young pig--a review of the secretory antibody systems and their possible role in oral immunisation. Vet Rec 92(25), 658-664.
Saiada F, Eldemery F, Zegpi RA, Gulley SL, Mishra A, Santen VLV, & Toro H (2019) Early Vaccination of Chickens Induces Suboptimal Immunity Against Infectious Bronchitis Virus. Avian Dis 63(1), 38-47.
Shaban NS, Radi AM, Bogzil AH, El-Banna H, Mobarez EA, & El-Gendy A (2019) Effect of bromhexine on the pharmacokinetic of tilmicosin in broiler chickens. Biomed. Pharmacol. J 12(3), 1085-1093.
Shahabi-Ghahfarokhi B, Gholami-Ahangaran M, & Jafarian-Dehkordi M (2016) Aflatoxin effect on humoral and mucosal immune responses against infectious bronchitis vaccine in broilers. Thai J Vet Med 46(1), 149-153.
Smialek M, Tykalowski B, Dziewulska D, Stenzel T, & Koncicki A (2017) Immunological aspects of the efficiency of protectotype vaccination strategy against chicken infectious bronchitis. BMC Vet Res 13(1), 44.
Thompson G, Mohammed H, Bauman B, & Naqi S (1997) Systemic and local antibody responses to infectious bronchitis virus in chickens inoculated with infectious bursal disease virus and control chickens. Avian Dis 41(3), 519-527.
Toro H, & Fernandez I (1994) Avian infectious bronchitis: specific lachrymal IgA level and resistance against challenge. Zentralbl Veterinarmed B 41(7-8), 467-472.
van Ginkel FW, van Santen VL, Gulley SL, & Toro H (2008) Infectious bronchitis virus in the chicken Harderian gland and lachrymal fluid: viral load, infectivity, immune cell responses, and effects of viral immunodeficiency. Avian Dis 52(4), 608-617.
Yuta A, & Baraniuk JN (2005) Therapeutic approaches to mucus hypersecretion. Curr Allergy Asthma Rep 5(3), 243-251.
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