The role of oxidative biomarkers in feline pan- leukopenia

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Published: Jul 10, 2022
DOI: https://doi.org/10.12681/jhvms.26868
Keywords:
Antioxidant Feline pan-leukopenia Malondialdehyde Vitamin
A Khoshvaghti
Department of Clinical Sciences, Faculty of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran
https://orcid.org/0000-0002-8577-0439
E Nojaba
https://orcid.org/0000-0003-0420-1128
Abstract

Feline Pan-leukopenia is an important deadly gastrointestinal disease of cats. It is mostly prevalent in kittens with declining maternal antibodies as well as unvaccinated cats. Vitamin D and E, glutathione peroxidase and superoxide dismutase are essential enzymatic and non-enzymatic components of defensive system involve in defending the body against oxidative radicals. Malondialdehyde is a reliable commonly used marker of overall lipid peroxidation level showing the presence of oxidative stress. Measurement of the total serum antioxidant capacity seems to represent a suitable biochemical parameter for evaluating the overall antioxidant status resulting from antioxidant intake or production, and their consumption by the increasing levels of oxidative stress.

The purpose of this study was to investigate the changes in serum levels of Vitamins D and E, glutathione peroxidase, superoxide dismutase, total antioxidant capacity and malondialdehyde in cats infected with pan-leukopenia virus.

Ten Persian male cats with clinical and laboratory symptoms of pan-leukopenia (n=10) and ten healthy Persian male cats (n=10) with a mean age of 6±2 months (minimum and maximum age were 3 and 8 months respectively) were selected.  Superoxide dismutase, total antioxidant, malondialdehyde and glutathione peroxidase levels were assayed by ELISA, and vitamin E and D were measured by chromatography methods.

There were no statistically significant differences between the mean serum levels of vitamin E, superoxide dismutase and total antioxidant in healthy cats and those with feline pan leukopenia infection (P>0.05), while a statistically significant decrease in glutathione peroxidase and vitamin D levels, and an increase in malondialdehyde level were observed (P<0.05).

A condition of oxidative stress appears in feline pan-leukopenia, possibly associated with the virus infection. This oxidative stress may play a role in prevention/treatment of feline panleukopenia.
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References
Kruse BD,Unterer S, Horlacher K, Sauter-Louis C, Hartmann K. Prognostic Factors in Cats with Feline Pan-leukopenia. J Vet Intern Med 2010; 24:1271–1276.
Barrs VR. Feline pan-leukopenia a re-emergent disease. Vet Clin Small Anim 2019;1-20.
Scott FWx. Viral diseases -panleukopenia. In: Holzworth J, editor.Diseases of the cat, vol. 1. Philadelphia: W. B. Saunders 1967; 182–193.
Di Serio F,Li SH, Pallas V, Randles J. Taxonomy- ninth report of the international committee on taxonomy of Viruses. San Diego: Eelevier, Chapter 2012.
Scott Weese J,Elsevier GCE, Louis ST, Missour USA .Infectious diseases of the dog and cat (3rd ed) Can Vet J 2007;48(1): 75.
Foley JE, Orgad U, Hirsh DC, Poland A, Pedersen NC. Outbreak of fatal salm-onellosis in cats following use of a high-titer modified-livepanleukopenia virus vaccine.J Amer Med Assoc 1999 ;214:67–70, 43–64.
Addie DD, Jarrett O, Simpson J, Thompson H. Feline parvovirus in pedigree kittens. Vet Rec 1996; 138(5):119.
Mantione NL, Otto CM. Characterization of the use of an-tiemetic agents in dogs with parvoviral enteritis treated at aveterinary teaching hospital: 77 cases (1997–2000). J Amer Med Assoc 2005;227:1787–1793.
Kohn R, Chevion S, Schatz R, Berry EM. Evaluation of the total low molecular weight antioxidant activity of plasma in health and diseases: A new approach cell pharmacol. 1996;3:355-359.
Chapple ILC.Reactive oxygen species and antioxidants in inflammatory diseases. J Clin Period 1997; 24:287–296.
Cao G, Verdon CP,Wang H,Prior RL.Automated assay of oxygen radical absorbance capacity with the COBAS FARA II. Clin Chem 1995; 41: 1738–1744.
Halliwell B. Antioxidant and human disease: a general introduction. Nut Rev 1997; 544-552.
SIES H.Strategies of antioxidant defense. Eur J Biol Chem 1993; 215: 213-219.
Norusis MY. Spss for windows base system user’s guide release 6.0, 1st ed. Spss Inc, Michigon 1993; 281-290
Meister A, Anderson A, Anderson ME.Glutathione. Annu Rev Biochem 1983;52:711-60.
Chapple I LC, Mason GI, Garner I, Matthews JB, Thorpe GH, Maxwell SR, Whitehead TP. Enhanced chemiluminiscent assay for measuring the total antioxidant capacity of serum, saliva and cervicular fluid. Ann Clin Biochem 1997; 34: 412–421.
Kayar B, Dokuzeylul FM, Kandemir A, Kirbas A, Bayrakal ME .Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus.Vet Med 2015; 6 (5):274-281.
Aitken RJ. Patho- physiology of human spermatozoa. Curr Opin Obstet Gyn1994; 6:128-135.
Alvarez JG, Storey BT.Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Res 1989;23: 77-90.
Bajaja S, khan A .Antioxidants and diabetes. Indian J Endoc Metab 2012; 16(2): 267–271.
Mehdi W, zainvlabdeen JA, Mehde AA.Investigation of the antioxidant status in multiple myeloma patients: effects of therapy. Asian Pacific J Cancer Prev 2013;14 (6): 3663-3667.
Fengle LY, Xia S L. Role of antioxidant vitamins and elements in mastitis in dairy cow. J Adv Vet Anim Res2015; 2(1): 1-9.
Crnogaj M, Cerón JJ, Šmit I, Kiš I, Gotić J, Brkljačić M, Matijatko V, Peres Rubio C, Kučer N, Mrljak V.Relation of antioxidant status at admission and disease severity and outcome in dogs naturally infected with Babesia canis. BMC Vet Res 2017; 13:114.
Cave TA, Thompson W, Reid SW, Wadgson DR, Addie DD.Kitten mortality in the United Kingdom: a retrospective analysis of 274 histopathological examinations (1986 to 2000). Vet Rec 2002; 17: 497-501.
Langdon C, Langdon P. Handbook of Small Animal Practice. 4thed.Elsevier. 2008; 1087-1103.
Kruse BD,Unterer S,Horlacher K,Sauter‐Louis C,Hartmann K. Prognostic Factors in Cats with Feline Panleukopenia.J Vet Inter Med 2010;24(6):1271-1276.
Li Z-H, Zlabek V, Velisek J, Grabic R, Machova J, Randak T.Modulation of antioxidant defence system in brain of rainbow trout (Oncorhynchus mykiss) after chronic carbamazepine treatment. Comparative Biochemistry and Physiology Part C: Toxicol Pharmacol 2010 ;151:137-141.
Arthur JR, Boyne R .Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattle. Life Scim 1985; 22, 36(16):1569-75.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidise. J Lab Clin Med 1967; 70:158–169.
Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 1990; 186:407-21.
Nissen H , Kreysel H .Superoxide dismutase in human semen. Klinische Wochenschrift 1983;, 61:63-5.
Hussain SP,Amstad P, He P, Robles A. Lupold, S.p53-induced up-regulation of MnSOD and GPX but not catalase increases oxidative stress and apoptosis. Cancer Res 2004;64:2350-2356.
Karbownik M, Gitto E, Lewinski A, Reiter R. Induction of lipid peroxidation in hamster organs by the carcinogen cadmium: amelioration by melatonin. Cell Biol Toxicol 2001;17 :33-40.
Erikasson J, Kohvakka A. Magnesium and ascorbic acid supplementation in diabetes mellitus. Ann Nutr Metab 1995; 39: 217–223.
Barros PSM, Angelotti AC, Nobre F.Antioxidant profile of cataractous English Cocker Spaniels Vet Ophthalmology 1999; 2: 83–86.
Heliovarra M, Knekt P, Aho K, Aaran RK, Alfthan G, Aromaa A. Serum antioxidants and risk of rheumatoid arthritis.Ann Rheum Dis 1994; 53: 51–53.
Dringen R, Hamprecht B. Involvement of glutathione peroxidase and catalase in the disposal of exogenous hydrogen peroxide by altered astroglial cells. Brain Res 1997; 759 (1): 67-75.
Teclesa F,Caldínb M,Tvarijonaviciutea A, Escribano D,Martínez-Subiela S,Cerón JJ.Serum biomarkers of oxidative stress in cats with feline infectious peritonitis. Res Vet Sci 2015; 100(2015):12-17.
Fridovich I. The biology of oxygen radicals. Sci 1978; 201:875-80.
Irvine DS.Glutathione as a treatment for male infertility. Rev Reprod 1996;1:6-12.
Hetyey CS,Manczur F,Dudas-Gyorki Z,Reiczigel J,Ribiczey P,Vajdovich P,Voros K.Plasma Antioxidant Capacity in Dogs with Naturally Occurring Heart Diseases. J Vet Med Series A 2007; 54(1):36-39.
Baker TA, Milstien S, Katusic ZS .Effect of vitamin C on the availability tetrahydrobiopterin in human endothelial cells. J Cardiol Pharmacol 2001; 37:333-338
Jewell D, YU S, Joshi D. Effects of serum vitamin E levels on skin vitamin E level in dogs and cats. Vet Ther 2002; 3(3): 235-243.