Effects of fumonisin and Salmonella infection in the expression of Toll-like receptors in chicken ovary


Published: Nov 9, 2022
Updated: 2022-11-09
Keywords:
Mycotoxins Fumonisin B1 innate immunity Toll-like receptors
V Gkiorsos
https://orcid.org/0000-0001-9440-2366
G Michailidis
https://orcid.org/0000-0003-0624-7568
M Anastasiadou
https://orcid.org/0000-0001-8199-0146
I Giannenas
Abstract
Mycotoxins are secondary metabolites produced mainly by fungi that contaminate animal feed and basic food products throughout the world. Currently, more than 500 mycotoxins are reported and one of the most important concern to public health and agriculture is Fumonisin B1 (FB1). FB1, a mycotoxin produced by Fusarium moniliforme, is a contaminant of animal feed with various and complex cellular effects. Although FB1 has been associated with various diseases in animals, to date few studies have been performed to evaluate the endocrine disrupting effects of FB1 and more particularly the effects in the innate immune mechanisms of farm animals and more specifically in poultry species. As the family of Toll-like receptors (TLRs) is one of the key molecules of the innate immune system, the aim of the present study was to evaluate the transcriptional changes of TLRs in the chicken ovary in vivo, in chicks fed with FB1 and in response to Salmonella Enteritidis (SE) infection. RNA was extracted from the ovaries of sexually mature and aged birds, treated with FB1 and SE. Quantitative real-time PCR analysis revealed that FB1 and SE infection resulted in a significant down regulation of 5 TLR genes in the ovary of sexual mature and aged birds. These findings suggest that the mycotoxin FB1 suppresses the innate immune system of the chicken reproductive organs, through alteration in the expression of certain TLR genes.
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References
Akira S, Takeda K (2004) Toll-like receptor signalling. Nat Rev Immunol 4:499-511.
Albonico M, Schütz LF, Caloni F, Cortinovis C, Spicer LJ (2016). Toxicological effects of fumonisin B1 alone and in combination with other fusariotoxins on bovine granulosa cells. Toxicon 118:47-53.
Bondy GS, Pestka JJ, (2000) Immunomodulation by fungal toxins. J Toxicol Environ Health Part B Critical Reviews 3:109-143.
Boutigny AL, Beukes I, Small I, Zühlke A, Spiteller M, Van Rensburg BJ, Flett B, Viljoen A (2012) Quantitative detection of Fusarium pathogens and their mycotoxins in South African maize. 61(3):522-531.
Bryden WL, (2012) Mycotoxin contamination of the feed supply chain: Implication of animal productivity and feed security. Anim Feed Sci and Tech 173:134-158.
Buszewska-Forajta M (2020) Mycotoxins, invisible danger of feedstuff with toxic effect on animals. Toxicon 182:34-53.
Caloni F, Ranzenigo G, Cremonesi F, Spicer LJ (2009) Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells. Toxicon 54:337-344.
Collins TF, Shackelford ME, Sprando RL, Black TN, Láborde JB, Hansen DK, Eppley M, Trucksess MW, Howard PC, Bryant MA, Ruggles DI, Olejnik N, Rorie JI (1998) Effects of Fumonisin B1 in Pregnant Rats. Food Chem Toxicol 36:397-408.
Corrêa JAF, Orsoa PB, Bordinb K, Vaz Haraa R, Luciano FB (2018) Toxicological effects of fumonisin B1 in combination with other Fusarium toxins. Food Chem Toxicol 121:483-494.
Cortinovis C, Caloni F, Schreiber NB, Spicer LJ (2014) Effects of fumonisin B1 alone and combined with deoxynivalenol or zearalenone on porcine granulosa cell proliferation and steroid production. Theriogenology 81:1042-1049.
Dombrink-Kurtzman MA (2003) Fumonisin and beauvericin induce apoptosis in turkey peripheral blood lymphocytes. Mycopathologia 4:357-364.
Fernández-Blanco C, Frizzell C, Shannon M, Ruiz MJ, Connolly L (2016) An in vitro investigation on the cytotoxic and nuclear receptor transcriptional activity of the mycotoxins fumonisin B1 and beauvericin. Toxicol Lett 257:1-10.
Fink-Gremmels J (2008) The role of mycotoxins in the health and performance of dairy cows. Vet J 176(1):84-92.
Fung F, Richard C (2004) Health effects of mycotoxins: A toxicological overview. J Toxicol 42:217-234.
Garrido CE, Pezzani CH, Pacin A (2012) Mycotoxins occurrence in Argentina’s maize (Zea mays L.), from 1999 to 2010. Food Control 25(2):660-665.
Gbore FA (2009) Growth performance and puberty attainment in growing pigs fed dietary fumonisin B1. J Anim Physiol Anim Nutr 93:761-767.
Gbore FA, Owolawi TJ, Erhunwunsee M, Akele O, Gabriel-Ajobiewe RAO (2012) Evaluation of the reproductive toxicity of dietary fumonisin B1 in rats. Jordan J. Biol. Sci. 5:183-190.
Glenn AE (2007) Mycotoxigenic Fusarium species in animal feed. Anim Feed Sci Technol 137:213-240.
Guard-Petter J, (2001) The chicken, the egg and Salmonella enteritidis. Enviromental Microbiology, 3(7):421-430.
Haque MA, Wang Y, Shen Z, Li X, Saleemi MK, Cheng He (2020) Mycotoxin contamination and control strategy in human, domestic animal and poultry: A review. Microb Pathog 142:104095.
International Programme on Chemical Safety (2002) Global Assessment of the State-of the-Science of Endocrine Disruptors. World Health Organization, Geneva.
Li YC, Ledoux DR, Bermudez AJ, Fritshe KL, Rottinghaus GE (1999) Effects of Fumonisin B1 on Selected Immune Responses in Broiler Chicks. Poult Sci 78:1275-1282.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-ΔΔC[T]) method. Methods 25:402-408.
Marin DE, Taranu I, Pascale F, Lionide A, Burlacu R, Bailly JD, Oswald IP (2006) Sex-related differences in the immune response of weanling piglets exposed to low doses of fumonisin extract. Br J Nutr 95:1185-1192.
Martins FA, Ferreira FMD, Ferreira FD, Bando E, Nerilo SB, Hirooka EY, Machinski MJr (2012) Daily intake estimates of fumonisins in corn-based food products in the population of Parana, Brazil. Food Control 23:614-618.
Michailidis G, Theodoridis A, Avdi M (2010) Transcriptional profiling of Toll-like receptors in chicken embryos and in the ovary during sexual maturation and in response to Salmonella enteritidis infection. Anim Reprod Sci 122:294-302.
Moretti A, Pascale M, Logrieco AF (2019) Mycotoxin risks under a climate change scenario in Europe. Trends Food Sci Technol 84:38-40.
National Research Council (1985) Guide for the Care and Use of Laboratory Animals. Publication No. 85-23 (Rev.). Natl Institute of Health, Bethesda, MD.
National Research Council (1994) Nutrient Requirements of Poultry. 8th rev ed National Academy Press Washington DC.
Oswald IP, Marin DE, Bouhet S, Pinton P, Taranu I, Accensi F (2005) Immunotoxicological risk of mycotoxins for domestic animals. Food Addit Contam 22(4):354-360.
Pierron A, Alassane-Kpembi I, Oswald IP (2016) Impact of mycotoxin on immune response and consequences for pig health. Anim Nutr 2:63-68.
Pierron A, Alassane-Kpembi I, Oswald IP (2016) Impact of two mycotoxins deoxynivalenol and fumonisin on pig intestinal health. Porc Health Manag 2:21.
Pizzo F, Caloni F, Schreiber NB, Cortinovis C, Spicer LJ (2016) In vitro effects of deoxynivalenol and zearalenone major metabolites alone and combined, on cell proliferation, steroid production and gene expression in bovine small-follicle granulosa cells. Toxicon 109:70-83.
Ranzenigo G, Caloni F, Cremonesi F, Aad PY, Spicer LJ (2008) Effects of Fusarium mycotoxins on steroid production by porcine granulosa cells. Anim Reprod Sci 107:115-130.
Rottinghaus GE, Olsen B, Osweiler GD (1982) Rapid screening method for aflatoxin B1, zearalenone, ochratoxin A, T-2 toxin, diacetoxyscirpenol and vomitoxin. In: Proceedings of the 25th Annual American Association of Veterinary Laboratory Diagnosticians, Nashville, TN: pp 477-484.
Ukwuru MU, Ohaegbu CG, Muritala A (2018) An overview of mycotoxin contamination of foods and feeds. J Biochem Microb Toxicol 1:101
Yang C, Song G, Lim W (2020) Effects of mycotoxin-contaminated feed on farm animals. J Hazard Mater 389:122087.
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