Effects of estradiol, progesterone and kisspeptin-1 concentrations at the time of insemination on conception rates in cattle
Published:
Jul 11, 2023
Updated:
2023-07-11
Keywords:
Cow Estradiol Follicular Diameter Kiss-1 Pregnancy.
Abstract
The present study aimed to investigate the effects of kisspeptin (Kiss-1), estradiol (E2), and progesterone (P4) levels at the time of artificial insemination (AI) on conception rates and also whether or not there is a relationship between Kiss-1 and E2 levels in cattle. Cows (n=100) raised on a semi-closed farm system and having corpus luteum were administered 25mg of prostaglandin F2a (Dinolytic®, Zoetis, Spain) and were evaluated following injection for signs of estrus. Just prior to AI, pre-ovulatory follicle diameters were assessed by ultrasonography (USG) and recorded. Then blood samples were collected. Blood serum samples were separated and stored until analyzed. Pregnancies were determined with rectal USG on day 30±2 following AI. The mean follicular diameters at the time of AI were similar in pregnant (17.9±0.01 mm) and non-pregnant (17.9±0.01 mm) cows. E2, P4, and Kiss-1 levels at the time of AI were determined from serum samples using the ELISA method. The Kiss-1 level at the time of AI in pregnant cows (80.58±4.4 pg/mL) was statistically higher than that in non-pregnant cows (66.68±2.48 pg/mL) (P=0.003). There were no statistically significant differences in E2 and P4 levels between pregnant and non-pregnant cows. Moreover, while a significant correlation was found between serum kiss-1 level and follicle diameter, no significant correlation was found between serum estrogen and progesterone levels. In conclusion, Kiss-1 levels at the time of AI may be used to predict the possibility of successful pregnancy in cattle, allowing different approaches to be developed to increase pregnancy rates in animals with low Kiss-1 levels at the time of AI.
Article Details
- How to Cite
-
Kaya, S., Kaçar, C., Arı, U., Merhan, O., Demir, M., Bekyürek, T., & Gürbulak, K. (2023). Effects of estradiol, progesterone and kisspeptin-1 concentrations at the time of insemination on conception rates in cattle. Journal of the Hellenic Veterinary Medical Society, 74(2), 5533–5538. https://doi.org/10.12681/jhvms.27527 (Original work published July 4, 2023)
- Issue
- Vol. 74 No. 2 (2023)
- Section
- Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
Downloads
Download data is not yet available.
References
Bhalakiya N, Haque N, Patel P (2019) Kisspeptin: a novel regulator in
reproductive physiology. Int J Livest Prod 9 (7): 1-13. doi: 10.5455/
ijlr.20190222105709
Mishra GK, Patra MK, Singh LK, Upmanyu V, Chakravarti S, Karikalan
M, Singh SK, Das GK, Kumar H, Krisnaswami N (2019) Kiss1 and
its receptor: molecular characterization and immunolocalization in
the hypothalamus and corpus luteum of the buffalo. Anim biotechnol
: 342-351. doi: 10.1080/10495398.2018.1520715
Okamura H, Yamamura T, Wakabayashi Y (2013) Kisspeptin as a master
player in the central control of reproduction in mammals: an overview of kisspeptin research in domestic animals. Anim Sci J 84: 369-
doi: 10.1111/asj.12056
Naniwa Y, Nakatsukasa K, Setsuda S, Oishi S, Fujii N, Matsuda F, Uenoyama Y, Tsukamura H, Maeda K, Ohkura S (2013) Effects of fulllength kisspeptin administration on follicular development in Japanese Black beef cows. Reprod Dev 59 (6): 588-594. doi: 10.1262/
jrd.2013-064
Mondal M, Baruah KK, Prakash BS (2015) Determination of plasma
kisspeptin concentrations during reproductive cycle and different
phases of pregnancy in crossbred cows using bovine specific enzyme
immunoassay. Gen Comp Endocrinol 224: 168-175. doi: 10.1016/j.
ygcen.2015.08.014
Leonardi CEP, Dias FCF, Adams GP, Singh J (2018) Effect of Kisspeptin-10 on plasma luteinizing hormone concentrations and follicular dynamics during the luteal phase in cattle. Theriogenology 119:
-274. doi: 10.1016/j.theriogenology.2018.06.023
Leonardi CEP, Dias FCF, Adams GP, Araujo ER, Singh J (2020) Kisspeptin induces ovulation in heifers under low plasma progesterone
concentrations. Theriogenology 141: 26-34. doi: 10.1016/j.theriogenology.2019.08.033
Martino N, Rizzo A, Pizzi F, Dell’Aquila ME, Sciorsci RL (2015) Effects
of kisspeptin-10 on in vitro proliferation and kisspeptin receptor expression in primary epithelial cell cultures isolated from bovine placental cotyledons of fetuses at the first trimester of pregnancy. Theriogenology 83: 978-987. doi: 10.1016/j.theriogenology.2014.11.033
Daniel JA, Foradori CD, Whitlock BK, Sartin JL (2015) Reproduction
and beyond, kisspeptin in ruminants. J Anim Sci Biotechnol 6:23. doi:
1186/s40104-015-0021-4
Gianetti E, Seminara S (2008) Kisspeptin and KISS1R: a critical pathway
in the reproductive system. Reproduction 136: 295-301. doi: 10.1530/
REP-08-0091
Hashizume T, Saito H, Sawada T, Yaegashi T, Ezzat AA, Sawai K, Yamashita T (2010) Characteristics of stimulation of gonadotropin secretion by kisspeptin-10 in female goats. Anim Reprod Sci 118: 37-
doi: 10.1016/j.anireprosci.2009.05.017
Rizzo A, Ceci E, Guaricci AC, Sciorsci RL (2019) Kisspeptin in the early
post–partum of the dairy cow. Reprod Domest Anim 54: 195-198.
doi: 10.1111/rda.13325
Rizzo A, Piccinno M, Ceci E, Pantaleo M, Mutinati M, Roncetti M, Sciorsci RL (2018) Kisspeptin and bovine follicular cysts. Vet Ital 54:
-31. doi: 10.12834/VetIt.1014.5413.3
Ahmed AE, Saito H, Sawada T, Yaegashi T, Yamashita T, Hirata T,
Hashizuma T (2009) Characteristics of the stimulatory effect of kisspeptin-10 on the secretion of luteinizing hormone, follicle-stimulating hormone and growth hormone in prepubertal male and female
cattle. J Reprod Dev 55(6): 650-654. doi: 10.1262/jrd.20255
Perry GA, Smith MF, Lucy MC, Green JA. Parks TE, MacNeil MD, Roberts AJ, Geary TW (2005) Relationship between follicle size at insemination and pregnancy success. PNAS 102 (14): 5268-5273. doi:
1073/pnas.0501700102
Atkins JA, Smith MF, Wells KJ, Geary TW (2010) Factors affecting preovulatory follicle diameter and ovulation rate after gonadotropin-releasing hormone in postpartum beef cows. Part I: Cycling cows. J
Anim Sci 88: 2300-2310. doi:10.2527/jas.2009-2531
Sa´ Filho OG, Meneghetti M, Peres RFG, Lamb GC, Vasconcelos JLM
(2009) Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows II: Strategies and factors affecting
fertility. Theriogenology 72: 210-218. doi:10.1016/j.theriogenology.2009.02.008
Taniguchi Y, Kuwahara A, Tachibana A, Yano Y, Yano K, Yamamato Y,
Yamasaki M, Iwasa T, Hinokio K, Matsuzaki T, Irahara M (2017)
Intra-follicular kisspeptin levels are related to oocyte maturation and
gonadal hormones in patients who are undergoing assisted reproductive technology. Reprod Med Biol 16(4): 380-385. doi: 10.1002/
rmb2.12056
Latif R, Rafique N (2015) Serum kisspeptin levels across different phases
of the menstrual cycle and their correlation with serum oestradiol.
Neth J Med 73(4):175-178
