Acute phase proteins and oxidative stress before and after treatment in neonatal calves with sepsis caused by Escherichia coli K99
Résumé
This study aimed to detect changes in acute-phase proteins endothelin-1 (ET-1), pentraxin-3 (P-3), ceruloplasmin (Cp), haptoglobin (Hp), and albumin, in calves with diarrhea caused by Escherichia coli K99 neonatal sepsis before and after treatment. Diagnostic and prognostic clinical, biochemical, and hematological parameters were evaluated. The sepsis group was composed of 20 newborn calves which met neonatal sepsis criteria, did not receive any treatment, and were referred to the clinics within 24 hours after the detection of clinical findings. The control group consisted of 10 healthy calves for comparison. Clinical examinations were performed before (0th h) and after (7th d) treatment. Blood samples were drawn via the jugular vein from sick calves before and after treatment and once from control animals. The P-3, ET-1, Cp, Hp, and malondialdehyde (MDA) concentrations in the sepsis group before treatment were statistically higher than post-treatment and control group values (P<0.05); while the glutathione (GSH) concentration was statistically lower (P<0.05). In conclusion, the high pre-treatment concentrations of P-3, ET-1, Cp, Hp, and MDA in the sepsis group were of diagnostic importance. The approximation of these parameters after treatment to the mean values of the control group was also of prognostic value.
Keywords: Calf; Ceruloplasmin; Haptoglobin; Sepsis diagnostic biomarkers
Article Details
- Comment citer
-
Akyüz, E., Merhan, O., Sezer, M., Coşkun, M., Erkılıç, E., Batı, Y., Bozukluhan, K., Kuru, M., Şahin, M., & Gökce, G. (2025). Acute phase proteins and oxidative stress before and after treatment in neonatal calves with sepsis caused by Escherichia coli K99. Journal of the Hellenic Veterinary Medical Society, 76(3), 9419–9428. https://doi.org/10.12681/jhvms.32294
- Numéro
- Vol. 76 No 3 (2025)
- Rubrique
- Research Articles
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Références
Akgün, S., Ertel, N.H., Mosenthal, A., Oser, W., 1998. Postsurgical
reduction of serum lipoproteins: Interleukin-6 and the acute-phase
response. The Journal of Laboratory and Clinical Medicine 131,
-108.
Akyüz, E., 2020. Kars bölgesindeki neonatal buzağılarda sıklıkla
karşılaşılan ishal kaynaklı sepsis nedenleri. In: ‘Tüm Yönleri ile
Kuzey Doğu Anadolu Bölgesinde Hayvancılık’, (Ed.): Ayvazoğlu
Demir, P. İksad Yayınevi, Ankara, Türkiye, 401-423.
Akyüz, E., Kükürt, A., 2021. Evaluation of oxidative stress index and
some biochemical parameters in neonatal calves with diarrhea. Acta
Scientiae Veterinariae 3, 58-63.
Akyüz, E., Makav, M., Kuru, M., Bayram, P., 2021. The effect of tarantula
cubensis extract on gentamicin-induced acute kidney injury in
ovariectomized rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi
, 559-566.
Akyüz, E., Coşkun, A., Şen, İ., 2016. Deneysel endotoksemi oluşturulan
buzağılarda sıvı tedavisinin hemodinamik parametreler üzerine
etkisi. Eurasian Journal of Veterinary Sciences 32, 246-254.
Akyüz, E., Gökce, G., 2021. Neopterin, procalcitonin, clinical biochemistry,
and hematology in calves with neonatal sepsis. Tropical
Animal Health and Production 53, 354.
Aldridge, B.M., Garry, F.B., Adams, R., 1993. Neonatal septicemia in
calves: 25 cases (1985-1990). Journal of the American Veterinary
Medical Association 203, 1324-1329.
Asadi, A.H., Baghinezhad, M., Asadi, H., 2015. Neonatal calf diarrhea
induced by rotavirus and coronavirus: a review. International Journal
of Biosciences 6, 230-236.
Atakisi, E., Bozukluhan, K., Atakisi, O., Gokce, H.I., 2010. Total oxidant
and antioxidant capacities and nitric oxide levels in cattle
with traumatic reticuloperitonitis. Veterinary Record 167, 908-909.
Bachmann, L.M., Juni, P., Reichenbach, S., Ziswiler, H.R., Kessels,
A.G., Vogelin, E., 2005. Consequences of different diagnostic “gold
standards” in test accuracy research: Carpal tunnel syndrome as
an example. International journal of epidemiology 34, 953-955.
Başer, D.F., Civelek, T., 2013. Akut ishalli neonatal buzağılarda venöz
asit baz durumu ve renal fonksiyon arası korelasyon. Kocatepe
Veterinary Journal 6, 25-31.
Basoglu, A., Sen, I., Meoni, G., Tenori, L., Naseri, A., 2018. NMR-Based
plasma metabolomics at set intervals in newborn dairy calves with
severe sepsis. Mediators of Inflammation 8016510.
Beutler, E., Duron, O., Kelly, B., 1963. Improved method for the determination
of blood glutathione. The Journal of Laboratory and
Clinical Medicine 61, 882-888.
Beydilli, Y., Gökçe, H., 2019. Sepsisli neonatal buzağılarda bazı hematolojik
ve biyokimyasal parametrelerin araştırılması. Mehmet
Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 7, 55-67.
Bonelli, F., Meucci, V., Divers, T.J., Boccardo, A., Pravettoni, D., Meylan,
M., Belloli, A.G., Sgorbini, M., 2018. Plasma procalcitonin
concentration in healthy calves and those with septic systemic
inflammatory response syndrome. Veterinary Journal 234, 61-65.
Bozukluhan, K., Merhan, O., Gokce, H.I., Deveci, H.A., Gokce, G.,
Ogun, M., Marasli, S., 2017. Alterations in lipid profile in neonatal
calves affected by diarrhea. Veterinary world 10, 786-789.
Bozukluhan, K., Merhan, O., Gökçe, H.İ., Öğün, M., Atakişi, E.,
Kiziltepe, Ş., Gökçe, G., 2018. Determination of some acute phase
proteins, biochemical parameters and oxidative stress in sheep with
naturally infected Sheeppox virus. Kafkas Üniversitesi Veteriner
Fakültesi Dergisi 24, 437-441.
Bozukluhan, K., Merhan, O., Kiziltepe, Ş., Egritag, H.E., Akyuz, E.,
Gökçe, H.I., 2021. Determination of haptoglobin, some biochemical
and oxidative stress parameters in calves with pneumonia. Fresenius
Environmental Bulletin 30, 9492-9496.
Carpentier, Y.A., Scruel, O., 2002. Changes in the concentration and
composition of plasma lipoproteins during the acute phase response.
Current Opinion in Clinical Nutrition & Metabolic Care 5, 153-158.
Ceron, J.J., Eckersall, P.D., Martýnez-Subiela, S., 2005. Acute phase
proteins in dogs and cats: current knowledge and future perspectives.
Veterinary Clinical Pathology 34, 85-99.
Cerone, S.I., Sansinanea, A.S., Streitenberger, S.A., Garcia, M.C., Auza,
N.J., 2000. Cytochrome Coxidase, Cu, Zn-superoxide dismutase,
and ceruloplasmin activities in copper-deficient bovines. Biological
trace element research 73, 269-278.
Chatre, L., Verdonk, F., Rocheteau, P., Crochemore, C., Chrétien, F.,
Ricchetti, M., 2017. A novel paradigm links mitochondrial dysfunction
with muscle stem cell impairment in sepsis. Biochimica
et Biophysica Acta - Molecular Basis of Disease 1863, 2546-2553.
Çitil, M., 2003. Puerperal infeksiyonlu ve abomasum deplasmanlı ineklerde
serum amiloid A ve haptoglobin düzeyleri. Kafkas Üniversitesi
Veteriner Fakültesi Dergisi 9, 147-51.
Çitil, M., Gökçe, E., 2013. Neonatal septisemi. Turkiye Klinikleri Journal
of Veterinary Sciences 4, 62-70.
Colombo, J.P., Richterich, R., 1964. Zur bestimmung des caeruloplasmin
im plasma [on the determination of ceruloplasmin in plasma].
Schweizerische Medizinische Wochenschrift 23, 715-720.
Coşkun, A., Şen, İ., 2011. Sığırlarda akut faz proteinleri ve klinik kullanım
alanları. Sağlık Bilimleri Dergisi 20, 240-246.
Cray, C., 2012. Acute phase proteins in animals. Progress in Molecular
Biology and Translational Science 105, 113-150.
Dellinger, R.P., Levy, M.M., Rhodes, A., Annane, D., Gerlach, H., Opal,
S.M., Sevransky, J.E., Sprung, C.L., Douglas, I.S., Jaeschke, R.,
Osborn, T.M., 2013. Surviving sepsis campaign: International guidelines
for management of severe sepsis and septic shockCritical Care
Medicine 41, 580-637.
Dratwa-Chalupnık, A., Herosımczyk, A., Lepczyńskı, A., Skrzypcazk,
W.F., 2012. Calves with diarrhea and a water-electrolyte balance.
Medycyna weterynaryjna 68, 5-8.
Dupuy, A.M., Philippart, F., Pean, Y., Lasocki, S., Charles, P.E., Chalumeau,
M., Claessens, Y.E., Quenot, J.P., Gras-Le Guen, C., Ruiz,
S., Luyt, C.E., Roche, N., Stahl, J.P., Bedos, J.P., Pugin, J., Gauzit,
R., Misset, B., Brun-Buisson, C., 2013. Role of biomarkers in the management of antibiotic therapy: an expert panel review: I - currently
available biomarkers for clinical use in acute infections.
Annals of Intensive Care 3, 22.
Ercan, N., Tuzcu, N., Başbug, O., Tuzcu, M., Alim, A., 2016. Diagnostic
value of serum procalcitonin, neopterin, and gamma interferon in
neonatal calves with septicemic colibacillosis. Journal of Veterinary
Diagnostic Investigation 28, 180-183.
Erkılıç, E.E., Merhan, O., Kırmızıgül, A.H., Öğün, M., Akyüz, E., Çitil,
M., 2019. Salivary and serum levels of serum amyloid a, haptoglobin,
seruloplasmin and albumin in neonatal calves with diarrhoea.
Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25, 583-586.
Erkılıç, E.E., 2019. Babesiosis’li köpeklerde tedavi öncesi ve sonrası
haptoglobin, seruloplazmin ve bazı biyokimyasal parametrelerin
seviyelerinin belirlenmesi. Harran Üniversitesi Veteriner Fakültesi
Dergisi 8, 77-80.
Fecteau, G., Paré, J., Van, Metre, D.C., Smith, B.P., Holmberg, C.A.,
Guterbock, W., Jang, S., 1997. Use of a clinical sepsis score for
predicting bacteremia in neonatal dairy calves on a calf rearing
farm. Van Veterinary Journal 38, 101-104.
Fecteau, G., Smith, P.B., George, L.W., 2009. Septicemia and meningitis
in newborn calf. Veterinary Clinics of North America - Food
Animal Practice 25, 195-208.
Franck, S.M., Brad, T.B., Harley, W.M., 1998. Multiplex PCR for enterotoxigenic,
attaching and effacing, and shiga toxin-producing
escherichia coli strains from calves. Journal of Clinical Microbiology
, 1795-1797.
Gruys, E., Toussaint, M.J.M., Niewald, T.A., 2005. Acute phase reaction
and acute phase proteins. Journal of Zhejiang University: Science
, 1045-1056.
Heegaard, P.M.H., Godson, D.L., Toussaint, M.J.M., Tjornehoj, K.,
Larsen, L.E., Viuff, B., Ronsholt, L., 2000. The acute phase response
of haptoglobin and serum amyloid A (SAA) in cattle undergoing
experimental infection with respiratory syncytial virus. Veterinary
Immunology and Immunopathology 77, 151-159.
House, J.K., Smith, G.W., McGuirk, S.M., Gunn, A.A., Izzo, M., 2015.
Manifestations and management of disease in neonatal ruminants.
In: ‘Large Animal Internal Medicine’, (Ed): Smith BP, 5th edition,
Elsevier Saunders, St. Louis, U.S.A., 302-338.
Kao, S.J., Yang, H.W., Tsao, S.M., Cheng, C.W., Bien, M.Y., Yu, M.C.,
Bai, K.J., Yang, S.F., Chien, M.H., 2013. Plasma long pentraxin
(PTX3) concentration is a novel marker of disease activity in
patients with community-acquired pneumonia. Clinical Chemistry
and Laboratory Medicine 51, 907-913.
Köse, S.İ., Maden, M., 2013. Biyobelirteçler ve klinik kullanımları.
Dicle Üniversitesi Veteriner Fakültesi Dergisi 2, 1-8.
Libby, P., Ridker, P.M., Hansson, G.K., 2009. Inflammation in atherosclerosis:
from pathophysiology to practice. Journal of the American
College of Cardiology 54, 2129-38.
Lorenz, I., Fagan, J., More, S.J., 2011. Calf health from birth to weaning.
II. management of diarrhoea in pre-weaned calves. Irish veterinary
journal 64, 9.
Marcato, F., Van den Brand, H., Kemp, B., van Reenen, K., 2018.
Evaluating potential biomarkers of health and performance in veal
calves. Frontiers in Veterinary Science 5, 133.
McGrotty, Y.L., Knottenbelt, C.M., Ramsey, I.K., Reid, S.W.J., Eckersall,
P.D., 2003. Haptoglobin concentrations in a canine hospital
population. Veterinary Record 152, 562-564.
Meisner, M., 2005. Biomarkers of sepsis: clinically useful? Current
Opinion in Critical Care 11, 473-480.
Merhan, O., Bozukluhan, K., Gökçe, G., Yılmaz, O., 2016. İshalli buzağılarda
haptoglobin, seruloplazmin ve bazı biyokimyasal parametre
düzeylerinin araştırılması. Fırat Üniversitesi Sağlık Bilimleri
Veteriner Dergisi 30, 195-198.
Merhan, O., Bozukluhan, K., Gokce, H.I., 2017. Acute phase proteins
and biochemical and oxidative stress parameters in Hypoderma
spp. infested cattle. The Journal of the Hellenic Veterinary Medical
Society 68, 535-540.
Moore, R.E., Kirwan, J., Doherty, M.K., Whitfield, P.D., 2007. Biomarker
discovery in animal health and disease: The application of
post-genomic technologies. Biomarker Insights 2,185-196.
Murata, H., Shimada, N., Yoshioka, M., 2004. Current research on acute
phase proteins in veterinary diagnosis: an overview. Veterinary
Journal 168, 28-40.
Naseri, A., 2017. Echocardiographic assesment of left ventriculer
systolic function in calves with naturally occurring severe sepsis
and septic shock and changes in these functions related to applied
treatment; longitudinal study. Selçuk University Health Sciences
Institute, Konya, Türkiye.
Novelli, G., Ferretti, G., Poli, L., Pretagostini, R., Ruberto, F., Perrella,
S.M., Levi, S., Morabito, V., Berloco, P.B., 2010. Clinical results
of treatment of postsurgical endotoxin-mediated sepsis with polymyxin-
b direct hemoperfusion. Transplantation Proceedings 42,
-1024.
Ok, M., Güler, L., Turgut, K., Ok, U., Sen, I., Gündüz, I.K., 2009. The
studies on the etiology of diarrhae in neonatal calves and determination
of virulence gene markers of Escherichia coli strains by
multiplex PCR. Zoonoses Public Health 56, 94-101.
Ok, M., Yıldız, R., Hatipoğlu, F., Baspinar, N., Ider, M., Uney, K.,
Erturk, A., Durgut, M., Terzi, F., 2020. Use of intestine-related biomarkers
for detecting intestinal epithelial damage in neonatal calves
with diarrhea. American journal of veterinary research 81, 139-146.
Pardon, B., Deprez, P., 2018. Rational antimicrobial therapy for sepsis
in cattle in face of the new lagislation on critically important
antimicrobials. Vlaams Diergeneeskundig Tijdschrift 87, 37-46.
Parlakpınar, H., Örüm,M.H., Acet, A., 2013. İlaca bağlı nefrotoksisitede
serbest oksijen radikalleri. Fırat Üniversitesi Sağlık BilimleriTıp
Dergisi 27, 51-56.
Petersen, H.H., Nielsen, J.P., Heegard, P.M.H., 2004. Application of
acute phase protein measurements in veterinary clinical chemistry.
Veterinary Research 35, 163-187.
Pourjafar, M., Badiei, K., Nazifi, S., Naghib, S.M., 2011. Acute phase
response in holstein dairy calves affected with diarrhoea. Bhikhabhai
Jivabhai Vanijya Mahavidyalaya 14, 142-149.
Pradeep, M., 2014. Application of acute phase proteins as biomarkers
in modern veterinary practice. Indian Journal of Veterinary and
Animal Sciences Research 43, 1-13.
Radostits, O.M., Gay, C.C., Hinchcliff, K.W., Constable, P.D., 2006.
Veterinary Medicine, A textbook of the diseases of cattle, sheep,
goats, pigs and horses, 10th edition, Saunders Co, London.
Roosendaal, B., Wim, G., De Graaf, F.K., 1984. The nucleotide sequence
of the gene encoding the K99 subunit of enterotoxigenic Escherichia
coli. FEMS Microbiology Letters 22, 253-258.
Seifi, H.A., Mohri, M., Shoorei, E., Farzaneh, N., 2006. Using haematological
and serum biochemical findings as prognostic indicators
in calf diarrhoea. Comparative Clinical Pathology 15, 143-147.
Sen, I., Constable, P.D., 2013. General overview to treatment of strong
ion (metabolic) acidosis in neonatal calves with diarrhea. Eurasian
Journal of Veterinary Sciences 29, 114-120.
Shah, R., 2007. Endothelins in health and disease. European Journal of
Internal Medicine 18, 272-82.
Skinner, J.G., Brown, R.A., Roberts, L., 1991. Bovine haptoglobin
response in clinically defined field conditions. Veterinary Record
, 147-149.
Sönmezer, Ç.M., Tülek, N., 2015. Biomarkers in bacterial infections
and sepsis. Klimik Dergisi 28,96-102.
Tokoyuni, S., 1999. Reactive oxygen species-induced molecular damage
and its application in pathology. Pathology International 49, 91-102.
Trefz, F.M., Lorch, A., Feist, M., Sauter-Louis, C., Lorenz, I., 2013. The
prevalence and clinical relevance of hyperkalaemia in calves with
neonatal diarrhoea. Veterinary Journal 195, 350-356.
Tschaikowsky, K., Sagner, S., Lehnert, N., Kaul, M., Ritter, J., 2000.
Endothelin in septic patients: effects on cardiovascular and renal
function and its relationship to proinflammatory cytokines. Critical
Care Medicine 28, 1854-1860.
Tuna, G.E., Ulutaş, B., 2015. Hastalıkların biyobelirteçleri olarak akut
faz proteinleri. Turkiye Klinikleri Veterinary Sciences- Internal
Medicine Special Topics 1, 8-19.
Uzlu, E., Karapehlivan, M., Çitil, M., Gökçe, E., Erdoğan, H.M., 2010.
İshal semptomu belirlenen buzağılarda serum sialik asit ile bazı
biyokimyasal parametrelerin araştırılması. Yüzüncü Yıl Üniversitesi
Veteriner Fakültesi Dergisi 21, 83-86.
Yildiz, R., Beslek, M., Beydilli, Y., Özçelik, M.M., Biçici, Ö., 2018.
Evaluation of platelet activating factor in neonatal calves with sepsis.
Veteriner Hekimler Derneği Dergisi 89, 66-73.
Yoshioka, T., Kawada, K., Shimada, T., Mori, M., 1979. Lipid peroxidation
in maternal and cord blood and protective mechanisms.
American Journal of Obstetrics and Gynecology 135, 972-976.
