The antioxidant effect of Michauxia campanuloides on rat ovaries

Antioxidant Michauxia campanuloides ovarian PCNA rat

This study investigated how Michauxia campanuloides affects the Proliferating Cell Nuclear Antigen (PCNA) expression in granulosa cells, the ovarian histomorphology and serum total antioxidant capacity (TAC) in rats. Rats were divided into control (C), treatment 1 (T 1) and treatment 2 (T 2) groups. The rats in the T 1 and T 2 groups received aqueous extract of M. campanuloides at doses of 20 mg/kg/day and 40 mg/kg/day orally for 21 days, respectively. Serum TAC levels, follicles counts including primordial, primary, preantral, antral and atretic follicles, and PCNA expression in granulosa cells were evaluated. Numbers of preantral follicles increased in T 1 and T 2 groups compared to C group (P < 0.05). TAC and numbers of preantral and antral follicles increased in T 2 group compared to T 1 and C groups (P < 0.05). PCNA expression in granulosa cells was increased in T 2 group compared to T 1 and C groups (P < 0.01). In conclusion, treatment with M. campanuloides had positive effects on antioxidant activity, follicular dynamics, and PCNA expression of granulosa cell in rats.

Article Details
  • Section
  • Research Articles
Download data is not yet available.
Abdollahifar MA, Azad N, Sajadi E, Mofarahe ZS, Zare F, Moradi A, Rezaee F, Gholamin M, Abdi S (2019) Vitamin C restores ovarian follicular reservation in a Mouse model of aging. Anatomy & Cell Biology 52 (2) : 196-203.
Abuelo A, Hernandez J, Benedito JL, Castillo C (2015) The importance of the oxidative status of dairy cattle in the periparturient period: revisiting antioxidant supplementation. Journal of Animal Physiology and Animal Nutrition 99 (6) : 1003-1016.
Agarwal A, Gupta S, Sharma RK (2005) Role of oxidative stress in female reproduction. Reproductive Biology and Endocrinology 3 (1) : 28.
Al-Amiery AA, Al-Bayati RI, Saour KY, Radi MF (2012) Cytotoxicity, antioxidant, and antimicrobial activities of novel 2-quinolone derivatives derived from coumarin. Research on Chemical Intermediates 38 (2) : 559-569.
Asif M (2015) Chemistry and antioxidant activity of plants containing some phenolic compounds. Chemistry International 1 (1) : 35-52.
Can B, Atilgan R, Pala S, Kuloğlu T, Kiray S, Ilhan N (2018) Examination of the effect of ovarian radiation injury induced by hysterosalpingography on ovarian proliferating cell nuclear antigen and the radioprotective effect of amifostine: an experimental study. Drug Design, Development and Therapy 12: 1491–1500.
Chuffa LGA, Amorim JPA, Teixeira GR, Mendes LO, Fioruci BA, Pinheiro PFF, Seiva FRF, Novelli ELB, Júnior WM, Martinez M, Martinez FE (2011) Long-term melatonin treatment reduces ovarian mass and enhances tissue antioxidant defenses during ovulation in the rat.
Brazilian Journal of Medical and Biological Research 44 (3) : 217-223.
Ciani F, Cocchia N, Angelo D, Tafuri S (2009) Influence of ROS on ovarian functions. In: Wu B, Editor. New Discoveries in Embryology. 1rd ed, UK, London: pp. 41-73.
Ćilerdžić J, Stajic M, Vukojević J, Duletic-Lausevic S (2013) Oxidative stress and species of genus Ganoderma (higher Basidiomycetes). International Journal of Medicinal Mushrooms, 15 (1) : 21-28.
Dai XX, Duan X, Cui XS, Kim NH, Xiong B, Sun SC (2015) Melamine induces oxidative stress in mouse ovary. Plos one 10 (11) : 1-9.
Damboldt J (1978) Campanulaceae. In: Davis PH, Editor. Flora of Turkey and the East Aegean Islands. Vol. 6. 1rd ed, Edinburgh University Press, UK: Edingburg: pp. 2-64.
Fejes S, Kery A, Blazovics A, Lugasi A, Lemberkovics E, Petri G, Szöke E (1998) Investigation of the in vitro antioxidant effect of Petroselinum crispum (Mill.) Nym. ex AW Hill. Acta Pharmaceutica Hungarica 68 (3) : 150-156.
Güvenç A, Akkol EK, Hürkul MM, Süntar İ, Keleş H (2012) Wound healing and anti-inflammatory activities of the Michauxia L’Hérit (Campanulaceae) species native to Turkey. Journal of Ethnopharmacology 139 (2) : 401-408.
Hamidpour R, Hamidpour S, Hamidpour M, Shahlari M, Sohraby M, Shahlari N, Hamidpour R (2017) Russian olive (Elaeagnus angustifolia L.) : From a variety of traditional medicinal applications to its novel roles as active antioxidant, anti-inflammatory, anti-mutagenic and analgesic agent. Journal of traditional and complementary medicine 7 (1) : 24-29.
Hürkul MM, Köroğlu A (2019) A review on the ethnobotanical use, chemical content and biological activity of the campanulaceae family. Türk Farmakope 2019; 4 (3) : 70-84.
Khaki A, Fathı AF, Ahmadı AHR, Rastgar H, Rezazadeh SA (2009) Effects of Danae racemosa on Spermatogenesis in Rat. Journal of Medicinal Plants 3 (31) : 87-92.
Koca RH, Hürkul MM, Kurt S, Köroğlu A (2020) Wistar Albino Sıçanlarda Michauxia campanuloides L’Hér.’in Bazı Sperma Parametreleri Üzerine Etkisi. Atatürk Üniversitesi Journal of Veterinary Science 15 (2) : 138-144.
Köroglu A, Hürkul MM, Özbay Ö (2012) Antioxidant Capacity and Total Phenol Contents of Bifora radians Bieb. FABAD Journal of Pharmaceutical Sciences 37 (3) : 123.
Krishnaiah D, Sarbatly R, Bono A (2007) Phytochemical antioxidants for health and medicine a move towards nature. Biotechnology and Molecular Biology Reviews 2 (4) : 97-104.
Luximon-Ramma A, Bahorun T, Soobrattee MA, Aruoma OI (2002) Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extracts of Cassia fistula. Journal of Agricultural and Food Chemistry 50 (18) : 5042-5047.
Lykkesfeldt J, Svendsen O (2007) Oxidants and antioxidants in disease: oxidative stress in farm animals. The Veterinary Journal 173 (3) : 502-511.
Maritim A, Sanders A, Watkins IIIJ (2003) Diabetes, oxidative stress, and antioxidants: a review. Journal of Biochemical and Molecular Toxicology 17 (1) : 24-38.
Oktay K, Schenken RS, Nelson JF (1995) Proliferating cell nuclear antigen marks the initiation of follicular growth in the rat. Biology of reproduction 53 (2) : 295-301.
Özcan P, Fıçıcıoğlu C, Yıldırım ÖK, Özkan F, Akkaya H, Aslan İ (2015) Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats. Reproductive Biomedicine Online 31 (3) : 404-410.
Puppel K, Kapusta A, Kuczyńska B (2015) The etiology of oxidative stress in the various species of animals, a review. Journal of the Science of Food and Agriculture 95 (11) : 2179-2184.
Rajaei S, Alihemmati A, Abedelahi A (2019) Antioxidant effect of genistein on ovarian tissue morphology, oxidant and antioxidant activity in rats with induced polycystic ovary syndrome. International Journal of Reproductive BioMedicine 17 (1) : 1-12.
Sen S, Chakraborty R (2011) The role of antioxidants in human health, oxidative stress: diagnostics, prevention, and therapy. ACS Publications 1083 (1) : 1-37.
Sen S, Chakraborty R, Sridhar C, Reddy Y, De B (2010) Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. International Journal of Pharmaceutical Sciences and Research 3 (1) : 91-100.
Shi SR, Liu C, Pootrakul L, Tang L, Young A, Chen R, Cote RJ, Taylor CR (2008) Evaluation of the value of frozen tissue section used as “gold standard” for immunohistochemistry. American Journal of Clinical Pathology 129 (3) : 358-366.
Soleymanı MM, Nourafshan A, Hamta A, Moumenı HR, Abnousi MH, Mahmoudi M, Anvari M, Hazaveh M (2010) Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol. A Stereological Study. Iranian Journal of Reproductive Medicine, 2010; 8 (1) : 1-9.
Stier A, Reichert S, Massemin S, Bize P, Criscuolo F (2012) Constraint and cost of oxidative stress on reproduction: correlative evidence in laboratory mice and review of the literature. Frontiers in Zoology 9 (1) : 37.
Strzalka W, Ziemienowicz A, (2011) Proliferating cell nuclear antigen (PCNA) : a key factor in DNA replication and cell cycle regulation. Annals of Botany 107 (7) : 1127-1140.
Sugino N (2005) Reactive oxygen species in ovarian physiology. Reproductive Medicine and Biology 4 (1) : 31-44.
Tománek M, Chronowska E (2006) Immunohistochemical localization of proliferating cell nuclear antigen (PCNA) in the pig ovary. Folia Histochemica et Cytobiologica 44 (4) : 269-274.
Van Rijn CM, Krijnen H, Menting-Hermeling S, Coenen, AM (2011) Decapitation in rats: latency to unconsciousness and the ‘wave of death’. Plos one 6 (1) : 1-6.
Wang S, He G, Chen M, Zuo T, Xu W, Liu X (2017) The role of antioxidant enzymes in the ovaries. Oxidative Medicine and Cellular Longevity (2017) : 1-14.
Yener NA, Sinanoglu O, Ilter E, Celik A, Sezgin G, Midi A, Deveci U, Aksungar F (2013) Effects of spirulina on cyclophosphamide-induced ovarian toxicity in rats: biochemical and histomorphometric evaluation of the ovary. Biochemistry Research International (2013) : 1-7.
Zhong RZ, Zhou DW (2013) Oxidative stress and role of natural plant derived antioxidants in animal reproduction. Journal of Integrative Agriculture 12 (10) : 1826-1838.