The effects of cGnRH on gonadotropin secretion and hatching traits in Japanese quail

PDF
DOI: https://doi.org/10.12681/jhvms.29375
Keywords:
Egg Fertility Hatchability Quail Testosterone
HB ÇIFTCI
Department of Animal Science, School of Agriculture, Selçuk University, 42130Konya-Turkey
A AYGÜN
Department of Animal Science, School of Agriculture, Selçuk University, 42130Konya-Turkey
Abstract

The aim of this study was to measure the effect of chicken Gonadotropin Releasing Hormone-I (cGnRH-I) on male fertility potential. Male and female quails were placed in cages(1 male and 5 females) and devoted into four experimental groups: a) control  group - no injection, b) negative control group - 200 μl standard saline was injected, c) 5μg cGnRH group - 5μg cGnRH-I was injected and d) 20μg cGnRH group - 20μg cGnRH-I was injected. Each group was consisting of scattered six cages. In each cage only males were subcutaneously injected under the wing (3 injections - 1 week apart from each other). A fourth injection was administered at the end of egg collection and one hour later blood was collected and serum concentrations of Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), prolactin and testosterone were measured. Egg fertility, hatchability and weekly egg production per hen were also measured. Injection of cGnRH-I did not increase pituitary gonadotropin secretion (LH or FSH); however, serum LH concentration non-significantly reduced in negative control group. A significant decrease in serum testosterone concentration was observed in negative control group compared to 20 μg cGnRH injected group. Fertility and hatchability of total set eggs were lower in negative control group compared to other groups. Egg production in control group was significantly decreased, probably due to the non-significant suppression of prolactin. Embryonic mortality (hatchability of fertile eggs) non-significantly increased in control and negative control groups compared to GnRH injected groups. It seems that cGnRH has a positive effect on fertility and hatchability; however, more studies are needed with older males to confirm our findings.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Avital-CohenN, HeiblumR, Argov-ArgamanN, RosenstrauchA, ChaisehaY, Mobarkey N, Rozenboim I (2013) Age-related changes in gonadal and serotonergic axes of broiler breeder roosters. Domest Anim Endocrinol44:145-150.
Aygun A, Sert D, Copur G (2012) Effects of propolis on eggshell microbial activity, hatchability, and chick performance in Japanese quail (Coturnix coturnix japonica) eggs. Poult Sci 91: 1018-1025.
Barna J, Mézes M (1994) Evaluation of testosterone response to intramuscular injection of GnRH and its correlation with sperm quality parameters in cockerels. Acta Vet Hung42:481-485.
Bédécarrats GY, ShimizuM, Guémené D (2006) Gonadotropin Releasing Hormones and their Receptors in Avian Species. J Poult Sci43: 199-214.
Boswell T, Sharp PJ, Hall MR, Goldsmith AR (1995) Migratory fat deposition in European quail: a role for prolactin? JEndocrinol 146: 71-79.
Bruggeman V, Onagbesan O, Vanmontfort D, Berghman L, Verhoeven G, Decuypere E (1998) Effect of long-term food restriction on pituitary sensitivity to cLHRH-I in broiler breeder females. J Reprod Fertil114:267-276.
Camper PM, Burke WH (1977) The effect of prolactin on reproductive function in female Japanese quail (Coturnix coturnix japonica) . Poult Sci56:1130-1134.
Dawson A (2015) Annual gonadal cycles in birds: Modeling the effects of photoperiodon seasonal changes in GnRH-1 secretion. Front. Neuroendocrinol37: 52-64.
Dunn IC, Lewis PD, Wilson PW, Sharp PJ (2003) . Acceleration of maturation of FSH and LH responses to photostimulation in prepubertal domestic hens by oestrogen. Reproduction126:217-225.
Follet BK (1976) Plasma Follicle-Stimulating Hormone During Photoperiodically Induced Sexual Maturation in Male Japanese Quail. J Endocrinol69:117-126.
Goldsmith AR, Hall M (1980) Prolactin concentrations in the pituitary gland and plasma of Japanese quail in relation to photoperiodically induced sexual maturation and egg laying. Gen Comp Endocrinol42: 449-454.
Hattori A, Ishii S, WadaM (1986) Effects of two kinds of chicken luteinizing hormone-releasing hormone (LH-RH) , mammalian LH-RH and its analogs on the release of LH and FSH in Japanese quail and chicken. Gen Comp Endocrinol64:446-455.
Jensen T, Jamieson SE, Castro I, Gartrell B, Cockrem JF, Durrant B (2019) Serum prolactin and testosterone levels in captive and wild brown kiwi (Apteryx mantelli) during the prebreeding, breeding, and incubation periods. Zoo Biol38:316-320.
Kaprara A, Huhtaniemi IT (2018) The hypothalamus-pituitary-gonad axis: Tales of mice and men. Metab Clin Exp 86: 3-17.
King JA, Millar RP (1982) Structure of chicken hypothalamic luteinizing hormone-releasing hormone. II. Isolation and characterization. J Biol Chem257:10729-10732.
Krishnan KA, Proudman JA, Bolt DJ, Bahr JM (1993) Development of an homologous radioimmunoassay for chicken follicle-stimulating hormone and measurement of plasma FSH during the ovulatory cycle. Comp Biochem Physiol Comp Physiol105:729-734.
Li G, Sun DX, Yu Y, Liu WJ, TangSQ, ZhangY, WangYC, Zhang SL, Zhang Y (2011) Genetic effect of the follicle-stimulating hormone receptor gene on reproductive traits in Beijing You chickens. Poult Sci90: 2487-2492.
Matsuda K, Nakamura K, Shimakura S, MiuraT, KageyamaH, Uchiyama M, ShiodaS, AndoH (2008) Inhibitory effect of chicken gonadotropin-releasing hormone II on food intake in the goldfish, Carassius auratus. Horm Behav54: 83-89.
Millam JR, Burke WH, El Halawani ME (1984) Release of gonadotropin-releasing hormone from the Japanese quail hypothalamus in vitro. Gen Comp Endocrinol 53: 293-301.
Miyamoto K, Hasegawa Y, Minegishi T, Nomura M, Takahashi Y, Igarashi M, Kangawa K, Matsuo H (1982) Isolation and characterization of chicken hypothalamic luteinizing hormone-releasing hormone. Biochem Biophys Res Commun107:820-827.
Miyamoto K, Hasegawa Y, Igarashi M, Chino N, Sakakibara S, Kangawa K, Matsuo H (1983) Evidence that chicken hypothalamic luteinizing hormone-releasing hormone is [Gln8]-LH-RH. Life Sci32:1341-1347.
Miyamoto K, Hasegawa Y, Nomura M, Igarashi M, Kangawa K, Matsuo H (1984) Identification of the second gonadotropin-releasing hormone in chicken hypothalamus: evidence that gonadotropin secretion is probably controlled by two distinct gonadotropin-releasing hormones in avian species. Proc Natl Acad Sci USA 81: 3874-38748.
Nicholls TJ, Goldsmith AR, DawsonA (1988) Photorefractoriness in birds and comparison with mammals. PhysiolRev68: 133-176.
Ottinger MA, Duchala CS, Masson M (1983) Age-related reproductive decline in the male Japanese quail. Horm Behav 17: 197-207.
Ottinger MA, Kubakawa K, Kikuchi M, Thompson N, Ishii S (2002) Effects of Exogenous Testosterone on Testicular Luteinizing Hormone and Follicle-Stimulating Hormone Receptors During Aging. Exp Biol Med (Maywood) 227: 830-836.
Ottinger MA, Abdelnabi M, Li Q, Chen K, Thompson N, Harada N, Viglietti-Panzica C, Panzica GC (2004) The Japanese quail: a mod el for studying reproductive aging of hypothalamic systems. Exp Gerontol39: 1679-1693.
Perera AD, Follett BK (1992) Photoperiodic induction in vitro: the dynamics of gonadotropin-releasing hormone release from hypothalamic explants of the Japanese quail. Endocrinology131: 2898-2908.
Proudman JA, Scanes CG, Johannsen SA, Berghman LR, Camp MJ (2006) Comparison of the ability of the three endogenous GnRHs to stimulate release of follicle-stimulating hormone and luteinizing hormone in chickens. Domest Anim Endocrinol 31: 141-153.
Renzoni A (1970) The influence of prolactin on the reproductive activity of the Japanese quail, Coturnix coturnix japonica. J Reprod Fertil22:365-368.
Robinson JE, Follett BK (1982) Photoperiodism in Japanese quail: the termination of seasonal breeding by photorefractoriness. Proc R Soc Lond B Biol Sci215: 95-116.
Rosenstrauch A, Degen AA, Friedländer M (1994) Spermatozoa retention by Sertoli cells during the decline in fertility in aging roosters. Biol Reprod 50: 129-136.
Ubuka T, Bentley GE, Tsutsui K (2013) Neuroendocrine regulation of gonadotropin secretion in seasonally breeding birds. Front Neurosci7:38.
Urbanski HF (1984) Episodic release of LH in gonadectomised male Japanese quail. J Endocrinol100: 209-212.
Van Wambeke F (1984) Effect of semen storage time and number of spermatozoa inseminated on the fertility and hatchability of eggs from dwarf broiler breeder hens. Br Poult Sci 25: 583-587.
Vizcarra J, Kirby J, Kreider D (2010) Testis development and gonadotropin secretion in broiler breeder males. Poult Sci89: 328-334.
Weil S, Rozenboim I, Degen AA, Dawson A, Friedländer M, Rosenstrauch A. , 1999. Fertility decline in aging roosters is related to increased testicular and plasma levels of estradiol. Gen Comp Endocrinol 115: 23-28.
Most read articles by the same author(s)