Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels Heavy Metal Resistance in E.coli

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Published: Jul 9, 2023
Updated: 2023-07-09
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2023-07-09 (2)
DOI: https://doi.org/10.12681/jhvms.29632
Keywords:
Copper Mercury Manganese E.coli Resistance
B Celik
Department of Microbiology Faculty of Veterinary Medicine Istanbul University-Cerrahpasa
https://orcid.org/0000-0001-9122-0284
N Sipahi
Traditional and Complementary Medicine Applied and Research Center, Duzce University, Duzce
https://orcid.org/0000-0001-8915-3545
I Kekec
Department of Microbiology Faculty of Veterinary Medicine Istanbul University-Cerrahpasa
B Halac
Department of Microbiology Faculty of Veterinary Medicine Istanbul University-Cerrahpasa";
https://orcid.org/0000-0002-3067-9937
Abstract

One of the biggest problems in the modern world is environmental pollution. All organisms are affected by the resulting pollution, albeit at different levels. One of the most important causes of pollution is the stable and non-perishable compounds formed due to heavy metals used in a wide range of environments. Heavy metal pollution, especially in the seas, causes cumulative accumulations in aquatic life, food and other products obtained from aquatic life, in living things that consume these products, and related diseases. The most critical sign of heavy metal pollution in water is the resistance formed against heavy metals in bacteria living in these waters. To determine the resistance to heavy metals in bacteria, the presence of resistance to Copper, Mercury and Manganese heavy metals in E. coli isolated from 18 mussels and 16 shrimps was genotypically investigated. For this purpose, the presence of pcoR genes for the determination of copper resistance, merA for the determination of mercury resistance, and mntR for the determination of manganese resistance was investigated in both plasmid and genomic DNA. As a result of the study, resistance genes were detected against heavy metals in 31 (91.17%) of 34 E. coli isolates examined. The presence of the pcoR gene (copper resistance) was found in 2 isolates (5.88%), the merA gene (mercury resistance) in only one isolate (2.94%) and the mntR gene (manganese resistance) in 8 isolates (23.52%). While pcoR and merA genes were not observed together in any isolates, pcoR and mntR genes were detected together in the genetic material of 10 isolates (29.41%). In comparison, mntR and merA genes were detected together in the genetic material of 7 isolates (20.58%). In 3 samples, all of the resistance genes against heavy metals were detected (8.82%).

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References
Abou-Shanab RAI, Berkum VP, Angle JS (2007). Heavy metal resistance
and genotypic analysis of metal resistance genes in Gram positive and
Gram negative bacteria present in Ni-rich serpetine soil and in the
rhizosphere of Alyssum murale. Chemosphere 68(2): 360-367.
Adekanmbi AO, Adelowo OO, Okoh AI, Fagade OE (2019). Metal-resistance encoding gene-fingerprints in some bacteria isolated from
wastewaters of selected printeries in Ibadan, South-western Nigeria. J
Taibah Univ 13(1): 266-273.
Aljerf L, AlMasri N (2018). A gateway to metal resistance: bacterial response to heavy metal toxicity in the biological environment. Annals
of Advances in Chemistry 2: 32-44.
Argudín MA, Hoefer A, Butaye P (2019). Heavy metal resistance in bacteria from animals. Vet Sci Res J 122: 132-147.
Azam M, Jan AT, Kumar A, Siddiqui K, Mondal AH, Haq QM (2018).
Study of pandrug and heavy metal resistance among E. coli from
anthropogenically influenced Delhi stretch of river Yamuna. Braz J
Microbiol 49 (3): 471-480.
Baker-Austin C, Wright SM, Stepanauskas R, McArthur VJ (2006).
Co-selection of antibiotic and metal resistance. Trends Microbiol. 14
(4): 177-182.
Bayne BL (2009). 1st Ed.Marine mussels: their ecology and physiology.
Cambridge University Press 1976; 528.
Binish MB, Shini S, Sinha RK, Krishnan KP, Mohan M (2021). Mercuric
reductase gene (merA) activity in a mercury tolerant sulphate reducing bacterium isolated from the Kongsfjorden, Arctic. Polar Sci 30,
Bukowski M, Piwowarczyk R, Madry A, Zagorski-Przybylo R., Hydzik
M et al. (2019). Prevalence of antibiotic and heavy metal resistance
determinants and virulence-related genetic elements in plasmids of
Staphylococcus aureus. Front. Microbiol 10, 805.
Chakraborty P, Mukhopadhyay M, Shruthi R, Mazumdar D, Snow D et
al. (2019). Biochar for Effective Cleaning of Contaminated Dumpsite
Soil: A Sustainable and Cost-Effective Remediation Technique for
Developing Nations. In: Environmental Biotechnology: For Sustainable Future 1st ed Springer, Singapore: pp: 3-29.
Chapman JS (2003). Disinfectant resistance mechanisms, crossresistance,
and co-resistance. Int Biodeterior Biodegradation 51 (4): 271–276.
Chihomvu P, Stegmann P, Pillay M (2015). Characterization and structure
prediction of partial length protein sequences pcoA, pcoR and chrB,
genes from heavy metal resistant bacteria from the Klip River, South
Africa. Int J Mol Sci 16 (4): 7352-7374.
Dahanayake PS, Hossain S, Wickramanayake MVKS, Heo GJ (2019).
Antibiotic and heavy metal resistance genes in Aeromonas spp. isolated from marketed Manila Clam (Ruditapes philippinarum) in Korea. J Appl Microbiol 127(3): 941-952.
De Silva B C, Hossain S, Dahanayake PS, He, GJ (2018). Frozen whiteleg shrimp (Litopenaeus vannamei) in Korean markets as a source
of Aeromonas spp. harboring antibiotic and heavy metal resistance
genes. Microb Drug Resist 24(10): 1587-1598.
Di Cesare A, Eckert EM, D’Urso S, Bertoni R, Gillan DC et al. (2016).
Co-occurrence of integrase 1, antibiotic and heavy metal resistance
genes in municipal wastewater treatment plants. Water Res 94: 208-
Dweba CC, Zishiri OT, El Zowalaty ME (2018). Methicillin-resistant
Staphylococcus aureus: livestock-associated, antimicrobial, and
heavy metal resistance. Infect Drug Resist, 11, 2497.
Eggers S, Safdar N, Malecki KM (2018). Heavy metal exposure and nasal
Staphylococcus aureus colonization: analysis of the National Health
and Nutrition Examination Survey (NHANES). J Environ Health,
(1): 1-10.
Hao X, Lüthe FL, Qin Y, McDevitt SF, Lutay N et al. (2015). Survival in amoeba-a major selection pressure on the presence of bacterial
copper and zinc resistance determinants? Identification of a copper
pathogenicity island. Appl Microbiol Biotechnol 99: 5817-5824.
Hernández-Ramírez KC, Reyes-Gallegos RI, Chávez-Jacobo VM, DíazMagaña A, Meza-Carmen V et al. (2018). A plasmid-encoded mobile
genetic element from Pseudomonas aeruginosa that confers heavy
metal resistance and virulence. Plasmid, 98: 15–21.
Hölzel CS, Müller C, Harms KS, Mikolajewski S, Schafer S et al. (2012).
Heavy metals in liquid pig manure in light of bacterial antimicrobial
resistance. Environ Res, 113(2012): 21-27.
Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN
(2014). Toxicity, mechanism and health effects of some heavy metals.
Interdiscip Toxicol 7(2): 60.
Jensen J, Kyvsgaard NC, Battisti A, Baptiste KE (2018). Environmental
and public health related risk of veterinary zinc in pig production-Using Denmark as an example. Environ Int 114: 181-190.
Jiang H, Yu T, Yang Y, Yu S, Wu J et al. (2020). Co-occurrence of antibiotic and heavy metal resistance and sequence type diversity of Vibrio parahaemolyticus isolated From Penaeus vannamei at freshwater
farms, seawater farms, and markets in Zhejiang Province, China.
Front Microbiol 11: 1294.
Li R, Wu H, Ding J, Li N, Fu W et al. (2020). Transgenic merA and merB
expression reduces mercury contamination in vegetables and grains
grown in mercury-contaminated soil. Plant Cell Rep 39(10): 1369-
Martinez JL, Sánchez MB, Martínez-Solano L, Hernandez A, Garmendia
L et al. (2009). Functional role of bacterial multidrug efflux pumps
in microbial natural ecosystems. FEMS Microbiol Rev 33: 430–449.
Martins VV, Zanetti MOB, Pitondo-Silva A, Stehling EG.(2014). Aquatic
environments polluted with antibiotics and heavy metals: a human
health hazard. Environ Sci Pollut Res 21(9): 5873-5878.
Miloud SB, Dziri O, Ferjani S, Ali MM, Mysara M et al. (2021). First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia. Pol J Microbiol 70(2): 161.
Mishra S, Bharagava RN, More N, Yada, A, Zainith S et al (2019). Heavy
metal contamination: an alarming threat to environment and human
health. In Environmental biotechnology: For sustainable future 1st ed.
Springer, Singapore: pp 103-125.
Misra TK, Brown NL, Fritzinger DC, Pridmore RD, Barnes WM et al.
(1984). Mercuric ion-resistance operons of plasmid R100 and transposon Tn501: the beginning of the operon including the regulatory
region and the first two structural genes. PNAS, 81(19): 5975-5979.
Moller AK, Barkay T, Hansen MA, Norman A, Hansen LH, et al. (2014).
Mercuric reductase genes(merA) and mercury resistance plasmids
in High Arctic snow, freshwater and sea-ice brine. FEMS Microbiol
Ecol 87: 52-63.
Paruthiyil S, Pinochet-Barros A, Huang X, Helmann JD (2019). Bacillus
subtilis TerC family proteins help prevent manganese intoxication. J
Bacteriol 202(2): e00624-19.
Patzer IS, Hantke K (2001). Dual repression by Fe2+ -Fur and Mn2+
-MntR of the mntH gene, encoding NRAMP-Like Mn2+ transporter
in Escherichia coli. J Bacteriol 183: 4806-4813.
Peng ED, Lyman LR, Schmitt MP (2021). Analysis of the Manganese and
MntR Regulon in Corynebacterium diphtheriae. J Bacteriol 203(20):
e00274-21.
Rasmussen LD, Zawadsky C, Binnerup SJ, Øregaard G, Sørensen SJ
(2008). Cultivation of hard-to-culture subsurface mercury-resistant
bacteria and discovery of new merA gene sequences. Appl Environ
Microbiol 74(12): 3795-3803.
Seiler C, Berendonk TU (2012). Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and
aquaculture. Front Microbiol 3: 399.
Sforzini S, Oliveri C, Orrù A, Chessa G, Pacchioni B et al (2018). Application of a new targeted low density microarray and conventional
biomarkers to evaluate the health status of marine mussels: A field
study in Sardinian coast, Italy. Sci Total Environ 628: 319-328.
Sipahi N, Karakaya E, İkiz S (2019). Phenotypic and genotypic investigation of the heavy metal resistance in Escherichia coli isolates
recovered from cattle stool samples. Turkish J Vet Anim Sci 43(5):
-691.
Staehlin BM, Gibbons JG, Rokas A, O’Halloran TV, Slot JC (2016). Evolution of a heavy metal homeostasis/resistance island reflects increasing copper stress in Enterobacteria. GBE 8(3):811-8
Terzi E & Civelek F (2021). Cu, Cd, As and Hg resistance levels in Escherichia coli isolated from Mediterranean mussel and sea snail in the
Southeastern Black Sea. J Mar Sci Technol, 10(1):36-41.
Trajanovska S, Britz LM, Bhave M (1997). Detection of heavy metal ion
resistance genes in Gram-positive and Gram-negative bacteria isolated from a lead contaminated site. Biodegradation, 8: 113-124.
Ture M, Altinok I, & Alp H (2018). Effects of cage farming on antimicrobial and heavy metal resistance of Escherichia coli, Enterococcus
faecium, and Lactococcus garvieae. Microb Drug Resist 24(9): 1422-
Uncumusaoglu AA, Sengul U, & Akkan T (2016). Environmental contamination of heavy metals in the Yaglidere Stream (Giresun), southeastern Black Sea. Fresenius Environ Bull 25(12): 5492-5498.
Walsh C, Caslake LF (2016). Conjugative competency of mercury resistant bacteria isolated from Onondaga Lake, NY. J Pa Acad Sci
(2):48-55.
Wang H, Wang Z, Yue R, Gao F, Ren R, et al. (2020). Rapid preparation
of adsorbent based on mussel inspired chemistry and simultaneous
removal of heavy metal ions in water. Chem Eng J 383:123107.
Xavier JC, Costa PES, Hissa DC, Melo VMM, Falcão RM, et al. (2019).
Evaluation of the microbial diversity and heavy metal resistance
genes of a microbial community on contaminated environment. J
Appl Geochem 105:1-6.
Yang S, Deng W, Liu S, Yu X, Mustafa GR, et al. (2020). Presence of
heavy metal resistance genes in Escherichia coli and Salmonella isolates and analysis of resistance gene structure in E. coli E308. J Glob
Antimicrob Resist 21: 420-426.
Yazdankhah S, Rudi K, Bernhoft A (2014). Zinc and copper in animal feed
- development of resistance and co-resistance to antimicrobial agents
in bacteria of animal origin. Microb Ecol Health Dis, 25:25862
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