Effects of α-Tocopherol on chilled quality parameters of Tharparkar bull semen extended in Lecithin and Tris based egg yolk extender

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Published: Jan 18, 2024
Updated: 2024-01-18
DOI: https://doi.org/10.12681/jhvms.31491
Keywords:
Cryopreservation Tharparkar Bull Semen Chilled Semen α-tocopherol Lecithin based extender
A Channo
Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, 70060
A kaka
Deparent of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam
AA Memon
Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, 70060
MC Malhi
Department of Veterinary Physiology and Biochemistry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, 70060
https://orcid.org/0000-0001-6492-3160
M Bakhsh
University of Veterinary and Animal Sciences, CVAS Jhang Campus, 35200, UVAS Lahore, Pakistan
Q Kalwar
Department of Theriogenology, Faculty of Animal Sciences, Shaheed Benazir Bhutto University and Animal Sciences, Sakrand, 67210
DH Kalhoro
Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, 70060
A Sethar
Livestock Breeding Services Authority (LBSA) Sindh, Livestock Fisheries Department Government of Sindh
RA Jariko
Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, 70060
Abstract

In this experiment, four healthy Tharparkar Cattle Bulls, 4-5 years aged were used. Total 52 (n=13) ejaculates were collected, and the semen samples having motility, morphology and viability ≥70% were processed and extended into BIOXcell­­­TM and Tris Based Egg Yolk extender supplemented with and without α-tocopherol (Vitamin E) 0.02 Mm/ml. Pre-thawed assessment of motility, morphology, viability and membrane integrity with BIOXcell­­­TM + α-tocopherol (Vitamin E) (79.09±0.87, 86.13±0.79, 82.13±1.20, 81.40±0.77) showed improved quality parameters as compared to Tris+ α-tocopherol (Vitamin E) (74.69±0.94, 82.81±1.00, 77.63±1.26, 77.31±1.01), BIOXcellTM (73.55±1.20, 80.81±0.96, 76.02±1.28, 75.31±1.20) and Tris (71.72±1.28, 78.90±1.01, 74.04±1.31, 73.18±1.29) respectively. In conclusion supplementation of α-tocopherol (Vitamin E) improves the Pre thawed quality parameters i.e. Motility, Morphology, Viability and Membrane Integrity of Tharparkar Bull semen extended in BIOXcell­­­TM and Tris Based Egg Yolk extender.

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