Associations Between DGAT1/Alu1 Gene Polymorphismand Some Performance in Morkaraman andTushin Sheep

PDF
Published: Apr 20, 2024
DOI: https://doi.org/10.12681/jhvms.34028
Keywords:
DGAT1 Morkaraman Tushin polymorphism PCR-RFL
S Kopuzlu
Department of Animal Science, College of Agriculture, Ataturk University, Turkey
İ Aydın
Department of Animal Science, Graduate School of Natural and Applied Sciences, Atatürk University, Turkey
Z Sönmez
Department of Agricultural Biotechnology, College of Agriculture, Ataturk University, Turkey
H Ünal
Department of Animal Science, Graduate School of Natural and Applied Sciences, Atatürk University, Turkey
D Türkyılmaz
Ü Türkyılmaz
Abstract

DGAT is a microsomal enzyme that plays a central role in the biosynthesis of cellular triacylglycerols. The DGAT1 gene, which is involved in fatty acid metabolism, is accepted as a candidate gene for improving meat quality characteristics in sheep. In Morkaraman and Tuj sheep, The objective of the current research was to examine the genotypes of the myostatin gene and reveal the associations between the genotypes and some performance traits such as birth weight, weaning weight, weaning age, average daily weight gain, and productivity. Genotypes were determined by the PCR-RFLP method using the Alu1 restriction enzyme, and the genotype frequencies of Morkaraman and Tushin sheep were found to be 50% and 53%; 38.7% and 38%; 11.3% and 9% for CC, CT, and TT in the DGAT1 locus, respectively. It was defined that the C and the T allele frequencies were determined as 69% and 31% for Morkaramans and 72% and 28% for Tuj sheep, respectively. It was observed that the distribution of the DGAT1 gene locus examined in both breed populations was in balance according to the HW genetic equilibrium test. The association analysis revealed no statistically significant impact of the DGAT1 gene polymorphism in exon 3 on birth weight, weaning weight, weaning age, productivity, and daily live weight gain (P>0.05). DGAT1 gene showed polymorphisms in Morkaraman and Tushin sheep and can be regarded as a genetic marker for both sheep breed selection according to the association analysis results.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Abousoliman I, Reyer H, Oster M, Murani E,Mourad M, Rashed MA, Mohamed I,Wimmers K (2020)Analysis of CandidateGenesforGrowthandMilkPerformanceTraits in theEgyptianBarkiSheep. Animals, 10(197): 1-13.
Ala Noshahr F,Rafat A (2014)Polymorphism of DGAT1 gene anditsrelationshipwithcarcassweightanddressingpercentage in Moghanisheepbreed. Iran J.Appl. Anim.Sci., 4(2): 331-334.
Altwaty NH, Salem LM, Mahrous KF (2020)Singlenucleotidepolymorphisms in thegrowthhormonereceptor gene and Alu1 polymorphisms in thediacylglycerolacyltransferase 1 gene as relatedtomeatproduction in sheep. Veterinary World, 13(5): 884.
Anonymous (2009)TurkeyDomesticAnimalGeneticResources. https://www.tarimorman.gov.tr/TAGEM/Belgeler/yayin/Katalog%20
Türkçe.pdf [accessed 05 May 2021].
Bal O,Akyüz B (2014)Detection of Diacylglycerol O-Acyltransferase 1 (DGAT1) Gene Polymorphismby PCR-RFLP Method in East AnatolianRedandNative Black CattleBreeds at Villages. Journal of Faculty of VeterinaryMedicine, Erciyes University, 11(1): 7-13.
Bayraktar M,Shoshin O (2022)Estimation of theassociationsbetween GH and DGAT1 genesandgrowthtraitsbyusinga decisiontree in Awassisheep. AnimalBiotechnology, 33(1): 167-173.
Bayram D, Akyüz B, Arslan K, Özdemir F, Aksel EG, Çınar, MU (2019) DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambsandtheireffects on liveweightsuptoweaning age. Journal of theKafkas University of VeterinaryFaculty, 25(1): 9-15.
Buhman KK, Smith SJ, Stone SJ, Repa,JJ, Wong JS, Knapp FF,Farese RV (2022) DGAT1 is not essentialfor intestinal triacylglycerolabsorptionorchylomicronsynthesis. Journal of BiologicalChemistry, 277(28): 25474-25479.
Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E, Farese RV (1998)Identification of a gene encoding an acylCoA: diacylglycerolacyltransferase, a keyenzyme in triacylglycerolsynthesis. Proceedings of theNational Academy of Sciences, 95(22): 13018-13023.
Curi RA, CharduloLAL, Arrigoni MDB, Silveira AC, de Oliveira HN (2011) Associationsbetween LEP, DGAT1 and FABP4 gene polymorphismsandcarcassandmeattraits in Neloreandcrossbredbeefcattle. LivestockScience, 135(2-3): 244-250.
Dekkers JC (2014) Commercial application of marker-and gene-assistedselection in livestock: strategiesandlessons. Journal of animalscience, 82(suppl_13), E313-E328.
Esenbuğa N, Dayıoğlu H, Karaoğlu M, Kopuzlu S, Macit M (1998) Evaluation of theUse of Morkaraman andIvesLambs in EarlyBreeding in Terms of HerdEfficiency (Fertilitycharacteristicsper 2. Fecunditysheep). Eastern Anatolia AgricultureCongress, 14-18 September 1998. (ProceedingsBook) Erzurum: 894-903.
Geliyi C, İlaslan M (1978)Fertility, MilkandWoolYields of MorkaramansRaised in Kars Province Karacaören Village. Kars Trial andProduction Station DirectoratePublicationNumber: 4, Kars.
Kumar R, Gupta S, Meena AS, Naqvi SMK, Kumar S (2016) Polymorphism in Exon 17 of Diacylglycerol Acyltransferase-1 {DGAT-1) Gene in IndianSheepBreeds. IndianJournal of Small Ruminants (The), 22(2): 170-173.
Macit M, Aksoy A (1996)Thecomparison of someimportantproductiontraits of AwassiandRed Karaman ewesreared in the semi-intensiveconditions at Atatürk UniversityAgriculturalResearchand Application Farm. TÜBİTAK, TurkıshJournalof Veterınary&AnımalScıences, 20:465-470.
Mahrous KF, Hassanane MS, Mordy MA, Shafey Hİ, Rushdi HE (2015) Polymorphism of somegenesassociatedwithmeat‐relatedtraits in Egyptiansheepbreeds. İranianJournal of AppliedAnimalScience, 5(3): 655-663.
Meena AS, Bhatt RS, Sahoo A, Kumar S (2016)Geneticpolymorphism of thediacylglycerolacyltransferase 1 (DGAT1) gene in Malpurasheep. IndianJournal of Small Ruminants, 22(1): 97-99.
Mohammadi H, Shahrebabak MM, Sadeghi M (2013)Associationbetweensinglenucleotidepolymorphism in theovine DGAT1 gene andcarcasstraits in two Iraniansheepbreeds. Animalbiotechnology, 24(3): 159-167.
Nanekarani S, Kolivand M, Goodarzi M(2016)Polymorphism of a mutation of DGAT1 gene in Lorisheepbreed. J AdvAgriTech, 3(1): 38-41.
Noshahr FA,Rafat A (2014)Polymorphism of DGAT1 gene anditsrelationshipwithcarcass weightanddressingpercentage inMoghanisheepbreed. IranianJournalAppliedAnimalScience, 4(2): 331-334.
Öner Y, Pullu M, Akın O, Elmacı C (2011)Investigation of Beta-lactoglobulin (β-LG) andBovineGrowthHormone (bGH) GenesPolymorphismsBy Using HaeIIIandMspIRestrictionEnzymes in Brown SwissandHolsteinBreedsReared in Bursa Region. Journal of theKafkas University of VeterinaryFaculty, 17 (3): 371-376.
Özdemir M,Dogru Ü(2005) Relationshipsbetweenkappa-kazein ˘ polymorphismandproductiontraits in Brown SwissandHolstein, J. Appl. Anim. Res., 27: 101-104.
Özmen O,Kul S (2016)Identification of novelSNPsand 10 bpdeletion of ovine DGAT1 gene ndtheirassociationwithmilkproductiontraits. ThaiJ VetMed. 2016. 46(2): 271-278.
Peakall R,Smouse PE (2012)GenAlEx 6.5: geneticanalysis in Excel. Populationgenetic software forteachingandresearch. An update. Bioinformatics, 28(19): 2537-2539.
Ranjbari M, Hashemi A, Mardani K, Darvishzadeh R (2012)Allelicpolymorphism of Makoeisheepcalpastatin gene identifiedbya polymerasechainreactionandsingle-strandconformationpolymorphism. J AgricSciTechnol, 14(3): 533-538.
Reis C, Navas D, Pereira M, Cravador A (2001)Growthhormonealsopolymorphismanalysis in ightPortuguesebovinebreeds. Archivos de zootecnia, 50(190): 41-48.
Sadeghi M, Mokhber M, Moradi-Shahrbabak M, Soltani V, Behroozlak M (2020)Study of DGAT1 Gene PolymorphismswithCarcassTraits in IranianZelandLori-BakhtiariSheepBreeds. IranianJournal of AppliedAnimalScience, 10(3): 501-507.
Sajad F, Reza Y,Shahram N (2014)Study Of DGAT-1 Gene PolymorphismInMehraban-SheepByPCR-RFLP Technique. New Cellular &MolecularBıotechnologyJournal, 4(16): 29-35,
Tăbăran A, Mihaiu M, Dan SD, Reget O, Pivariu B, Cordis I, Muresan C (2014)Identification of polymorphism in goatandsheep DGAT1 gene associatedwithmilkproductiontraits. Bulletin UASVM VeterinaryMedicine, 71(2): 281-286.
Tambasco DD, Paz CCP, Pereira AP, Alencar MM, Freitas AR, Coutinho LL, Packer IU,Regitano L.C.A (2013)Candidategenesforgrowthtraits in beefcattlecross. J. Anim. Breed. Genet. 120, 51-56.
TUİK (2022)Hayvansal Üretim İstatistikleri.https://data.tuik. gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatistikleri-Haziran-2022-45594. [accessed 30 November 2022].
Xu QL, Chen YL, Ma RX, Xue P (2008) Polymorphism of DGAT1 associatedwithintramuscularfat‐mediatedtenderness in sheep. Journal of theScience of FoodandAgriculture, 89(2): 232-237.
Yang JT, Zang RX, Liu WJ, Xu HW, Bai JL, Lu JX, Wu JP (2011)Polymorphism of a mutation of DGAT1 gene in fourChineseindigenoussheepbre 460-468
Most read articles by the same author(s)