Quorum Sensing System in Pathogenic Aeromonas hydrophila: Discovery of LuxRI Homologs ahyI and ahyR Genes Responsible for N-Acyl Homoserine Lactone Signal Molecules

PDF
Published: Oct 21, 2024
DOI: https://doi.org/10.12681/jhvms.34042
Keywords:
Interbacterial communication A. hydrophila Quorum sensing system ahyI and ahyR Genes PCR
N Filik
Süleyman Demirel Üniversitesi (Süleyman Demirel University)
https://orcid.org/0000-0003-4376-7298
E Önem
Suleyman Demirel University, Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Isparta, Turkey
https://orcid.org/0000-0002-7770-7958
A Kubilay
Isparta University of Applied Sciences, Faculty of Egirdir Fisheries, Isparta, Turkey
Abstract

ABSTRACT


In the pathogenesis of infections; intercellular communication system called Quorum Sensing System (QS), which regulates production of many virulence factors, is held responsible. QS genes of Aeromonas hydrophila strains that cause Motile Aeromonas Septicemia in fish was investigated by PCR method in reserach. AhyI and ahyR genes responsible for virulence of 20 A. hydrophila strains and A. hydrophila ATCC 7966 reference strain isolated from disease fish were investigated by Polymerase Chain Reaction (PCR). By PCR analysis, both ahyI and ahyR genes responsible for generation of virulence in management of QS were detected in all A. hydrophila strains. In A. hydrophila strains, it initiates disease process by preventing premature stimulation of immune system by critical gene expressions such as virulence factors in management of QS. Ultimately, QS is responsible for virulence of A. hydrophila, and thus bacteria manage disease process. Keeping diseases under control by blocking communication between them by inhibiting of QS, which is critical in disease formation, is an important strategy in fight against bacteria. With identification of QS and all members of system (AHLs, AI, QSI e.g.), early diagnosis of disease is brought to agenda and it is expected to break new ground in diagnosis of fish diseases. As a result, A. hydrophila secretes AHL signal molecule with LuxI and ahyI gene, and detects AHL signal molecule with LuxR and ahyR gene. In this study, both ahyI and ahyR genes responsible for pathogenicity and virulence in management of QS were detected in all A. hydrophila strains by PCR analysis using 20 bp long primers. Thus, it has been proven that pathogen bacteria A. hydrophila possesses LuxI/LuxR-Type QS.


 


Keywords: Interbacterial communication, A. hydrophila, Quorum sensing system, ahyI and ahyR Genes, PCR

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
Author Biography
N Filik, Süleyman Demirel Üniversitesi (Süleyman Demirel University)

Department: Department of Fish Diseases

 

References
Agger, W.A., McCormick, J.D. & Gurwith, M.J. (2018). Aeromonas hydrophila ilişkili ishalin klinik ve mikrobiyolojik özellikleri. Journal
Clinical Microbiology, 2(18), 22-26.
Aleksandra, D. (2010). The Dawn of the Living, Talking Robots. The Journal of Young Investigators: An Undergraduate, Peer-Reviewed Science Journal. 19(22), http://www.jyi.org. http://www.jyi.org/features/
ft.php?id=285. Erişim Tarihi:03.05.2010.
Austin, B., & Sharifuzzaman, S. M. (2022). Probiotics in aquaculture.
Austin, B. & Austin, D.A. (2016). Bacterial fish pathogens. 6. th edition.
Springer International Publishing, Switzerland, pp: 21-82, 161-321,
-396, 643-721.
Ausubel, F., Brent, R., Kingston, R., Moor, D., Seidman, J., Smith, J. &
Stauhle, K. (1988). Current Protocols in Molecular Biology. New
York: Wiley Intersciences, 1(2), 146.
Azevedo, F., Pereira, H., & Johansson, B. (2017). Colony PCR. PCR:
Methods and Protocols, 129-139.
Baskın, H. (2005). Mikroorganizmanın çevreye uyumu ve biyofilm: “quorum sensing” (çoğunluğu algılama). Klimik 2005 XII. Türk Klinik
Mikrobiyoloji ve İnfeksiyon Hastalıkları Kongresi. Dokuz Eylül
Üniversitesi Tıp Fakültesi Mikrobiyoloji ve Klinik Mikrobiyoloji
Anabilim Dalı, İzmir, 9-10.
Bayrakal, V. & Baskın, H. (2018). Quorum Sensing ve Biyofilm. Biyofilm
Enfeksiyonları. (1. Basım) 4-13. Editör: Sakarya S. Ankara: Türkiye
Klinikleri.
Boyen, F., Eeckhaut, V., Van Immerseel, F., Pasmans, F., Ducatelle, R.,
& Haesebrouck, F. (2009). Quorum sensing in veterinary pathogens:
mechanisms, clinical importance and future perspectives. Veterinary
microbiology, 135(3-4), 187-195.
Bruhn, J.B., Dalsgaard, I., Nielsen, K.F., Buchholtz, C., Larsen, J.L. &
Gram, L. (2005). Quorum sensing signal molecules (acylated homoserine lactones) in Gram negative fish pathogenic bacteria. Diseases of
Aquatic Organisms, 65(1), 43-52. https://doi.org/10.3354/dao065043
Cappuccino, J.G. & Sherman, N. (1992). Biochemical activities of microorganisms. In: Microbiology. A Laboratory Manual. The Benjamin/
Cummings Publishing Co. California, USA. 76 s.
Chadha, J., Harjai, K., & Chhibber, S. (2022). Revisiting the virulence
hallmarks of Pseudomonas aeruginosa: a chronicle through the perspective of quorum sensing. Environmental Microbiology, 24(6),2630-2656.
Chopra, A.K., Xu, X.J., Ribardo, D., Gonzalez, M., Kuhl, K., Peterson,
J.W. & Houston, C.W. (2000). The cytotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and
activates arachidonic acid metabolism in macrophages. Infection and
Immunity, 68(5), 2808-2818. https://doi.org/10.1128/iai.68.5.2808-
2000
Christy, G., Kusdawarti, R. & Handijatno, D. (2019). Determination of the
aerolysin gene in Aeromonas hydrophila using the polymerase chain
reaction (PCR) technique. In IOP Conference Series: Earth and Environmental Science, 236(1), 012097, IOP Publishing.
Chu, T., Ni, C., Zhang, L., Wang Q., Xiao J., Zhang, Y. & Liu, Q. (2015). A
quorum sensing-based in vivo expression system and its application in
multivalent bacterial vaccine. Microbial Cell Factories, 14, 37.
Fan, Q., Zuo, J., Wang, H., Grenier, D., Yi, L., & Wang, Y. (2022). Contribution of quorum sensing to virulence and antibiotic resistance in
zoonotic bacteria. Biotechnology Advances, 107965.
Fernández, L., Álvarez, B., Menéndez, A., Méndez, J. & Guijarro, J.A.
(2008). Molecular Tools for Monitoring Infectious Diseases in Aquaculture, Dynamic Biochemistry. Process Biotechnology and Molecular Biology, 2(1), 33-43.
Filik, N., (2020). Kültür Balıklarından İzole Edilen Aeromonas hydrophila
Suşlarında Fenolik Bileşenlerin Çevreyi Algılama Sistemi Üzerine İnhibisyon Etkisi ve Suşlar Arasındaki Klonal İlişkinin Pulsed Field Jel
Elektroforez Yöntemiyle Belirlenmesi. TC. Isparta Uygulamalı Bilimler Üniversitesi ISUBÜ Lisansüstü Eğitim Enstitüsü. Doktora Tezi.
Filik, F., (2019). Bazı Bakteriyel Balık Patojenlerinde Biyofilm Oluşumuna Farklı Maddelerin İn Vitro Etkisinin Tespiti. (Doctoral dissertation,
ISUBÜ Lisansüstü Eğitim Enstitüsü Enstitüsü). Yüksek Lisans Tezi.
Gupta, D. S., & Kumar, M. S. (2022). The implications of quorum sensing inhibition in bacterial antibiotic resistance-with a special focus on
aquaculture. Journal of Microbiological Methods, 106602.
Hellingwerf, K.J. (2005). Bacterial observations: a rudimentary from of
intelligence?. Trends in Microbiology, 13(4), 152-158.
Koch, R. (1884). Die Aetiologie der Tuberkulose. Mitt Kaiser Gesundh,
, 1-88.
Kozlova, E.V., Khajanchi, B.K., Popov, V.L., Wen, J. & Chopra, A.K.
(2012). Impact of QseBC System in cdi-GMP-Dependent Quorum
Sensing Regulatory Network in a Clinical Isolate SSU of Aeromonas hydrophila. Microbial Pathogenesis, 53, 115-124. https://doi.
org/10.1016/j.micpath.2012.05.008
Lynch, M.J., Swift, S., Kirke, D.F., Keevil, C.W., Dodd, C.E.R. & Williams, P. (2002). The regulation of biofilm development by quorum
sensing in Aeromonas hydrophila. Environmetal Microbiology, 4, 18-
Miller, M. B., & Bassler, B. L. (2001). Quorum sensing in bacteria. Annual
review of microbiology, 55(1), 165-199.
Nafiqoh, N., Novita, H., Sugiani, D., Gardenia, L., Taukhid, T., Widyaningrum, A., & Susanti, D. R. (2022). Aeromonas hydrophila AHL 0905-
and Streptococcus agalactiae N14G as Combined Vaccine Candidates for Nile Tilapia. Journal of Biosciences, 29(2), 137-145.
NCBI Genome Project, (2006). Aeromonas hydrophila subsp. hydrophila
ATCC 7966. http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genomeprj&cmd=ShowDetailView&TermToSearch=16697 (Son erişim tarihi: 16 Ekim 2019)
Nurcan, N. (2010). Bazı Gram-Negatif Bakteriyel Balık Patojenlerinde
Çevreyi Algılama Sisteminin İncelenmesi. (Yüksek Lisans Tezi, Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü)
Raffa, R.B., Iannuzzo, J.R., Levine, D.R., Saeid, K.K., Schwartz, R.C.,
Sucic, N.T., Terleckyj, O.D. & Young, J.M. (2005). Bacterial Communication (“Quorum Sensing”) via Ligands and Receptors: A Novel
Pharmacologic Target for the Design of Antibiotic Drugs. The Journal
of Pharmacology and Experimental Therapeutics, 312(2), 417-423.
Rahman, M. M., Rahman, M. A., Hossain, M. T., Siddique, M. P., Haque,
M. E., Khasruzzaman, A. K. M., & Islam, M. A. (2022). Efficacy of
bi-valent whole cell inactivated bacterial vaccine against Motile Aeromonas Septicemia (MAS) in cultured catfishes (Heteropneustes fossilis, Clarias batrachus and Pangasius pangasius) in Bangladesh. Saudi
Journal of Biological Sciences, 29(5), 3881-3889.
Rasko, D. & Sperandio, V. (2010). Anti-Virulence Strategies to Combat
Bacteria-Mediated Disease. Nature Reviews Drug Discovery, 9, 117-
Saeki, E. K., Martins, H. M., Camargo, L. C. D., Anversa, L., Tavares, E.
R., Yamada-Ogatta, S. F., ... & Nakazato, G. (2022). Effect of Biogenic
Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas
aeruginosa PAO1 and PA14. Microorganisms, 10(9), 1755.
Saraçlı, M.A. (2006). Quorum sensing: mikroorganizmalar iletişim mi kuruyor?. Gülhane Tıp Dergisi, 48(4), 244-250.
Sarkodie, E.K., Zhou, S. & Chu, W. (2019). N-Acylhomoserine Lactones
(AHLs), QseB/C Gene Detection, Virulence Factors and Antibiotics
Resistance of Aeromonas hydrophila. Advances in Microbiology, 9(5),
Sentromer, (2020). Sentromer DNA Teknolojileri Oligonükleotit Sentezleme Genel Bilgi. https://www.sentromer.com/ (Son erişim tarihi: 01
Ocak 2020)
Sequences, (2008). Bakteri hücreleri arasında haberleşme (Cell-to-cell
communication: quorumsensing). http://www.genotyping.wordpress.
com.http://genotyping.wordpress.com/2008/05/12/bakteri-hucreleri-arasindahaberlesme- cell-to-cell-communication-quorum-sensing/. (Son erişim tarihi: 28 Mart 2010)
Seshadri, R., Joseph, S.W., Chopra, A.K., Sha, J., Shaw, J., Graf,
J., Haft, D., Wu, M., Ren, Q., Rosovitz, M.J., Madupu, R., Tallon, L.,
Kim, M., Jin, S, Vuong, H., Stine, O.C., Ali, A., Horneman, A.J.
& Heidelberg,J.F. (2006). Genome sequence of Aeromonas hydrophila ATCC 7966T
: jack of all trades. Journal of Bacteriology, 188(23),
-8282. https://doi.org/10.1128/JB.00621-06
Smith, E.F. (1905). Bacteria in relation to plant disease. Science, 22(569),
Striednig, B., & Hilbi, H. (2022). Bacterial quorum sensing and phenotypic
heterogeneity: how the collective shapes the individual. Trends in Microbiology, 30(4), 379-389.
Swift, S., Karlyshev, A.V., Fish, L., Durant, E.L., Winson, M.K., Chhabra,
S.R., Williams, P., Macintyre, S. & Stewart, G.S.A.B. (1997). Quorum
Sensing in Aeromonas hydrophila and Aeromonas salmonicida: Identification of the LuxRI Homologs AhyRI and AsaRI and Their Cognate
N-Acylhomoserine Lactone Signal Molecules. Journal of Bacteriology, 179(17), 5271-5281.
Swift, S., Lynch, M.J., Fish, L., Kirke, D.F., Tomas, J.M., Stewart, G.S.A.B.
& Williams, P. (1999). Quorum Sensing-Dependent Regulation and
Blockade of Exoprotease Production in Aeromonas hydrophila. Infection and Immunity, 67(10), 5192-5199.
Swift, S., Throup, J.P., Williams, P., Salmond, G.P. & Stewart, G.S. (1994).
Quorum sensing: a population-density component in the determination
of bacterial phenotype. Trends in Biochemical Sciences, 21, 214-219.
Tanhay Mangoudehi, H., Zamani, H., Shahangian, S. S., & Mirzanejad, L.
(2020). Effect of curcumin on the expression of ahyI/R quorum sensing genes and some associated phenotypes in pathogenic Aeromonas
hydrophila fish isolates. World Journal of Microbiology and Biotechnology, 36, 1-9.
Tripathi, G., & Dhamotharan, K. (2022). Adverse Effects of Fish Vaccines.
In Fish immune system and vaccines (pp. 279-290). Springer, Singapore.
Watnick, P. & Kolter, R. (2000). Biofilm, City of Microbes. Journal of
Bacteriology, 182(10), 2675-2679.
Williams, P. (2007). Quorum Sensing, Communication and Cross-Kingdom Signalling in the Bacterial World. Microbiology, 153, 3923-3938.
Zhang, W., Zhao, J., Ma, Y., Li, J., & Chen, X. (2022). The effective components of herbal medicines used for prevention and control of fish
diseases. Fish & Shellfish Immunology.
Zhang, D., Pridgeon, J.W. & Klesius, P.H. (2013). Expression and activity
of recombinant proaerolysin derived from Aeromonas hydrophila cultured from diseased channel catfish. Veterinary Microbiology, 165(3-
, 478-482.
Zhu, J. & Winans, S.C. (2001). The quorum-sensing transcriptional regulator TraR requires its cognate signaling ligand for protein folding,
protease resistance, and dimerization. Proceedings of the National
Academy of Sciences USA, 98(4), 1507-1512. https://doi.org/10.1073/
pnas.98.4.1507
Most read articles by the same author(s)