Fasciolicidal efficacy comparison between Amaro (Chuquiraga weberbaueri) and triclabendazole in calves experimentally infected with Fasciola hepatica metacercaria

PDF
Published: Apr 18, 2025
DOI: https://doi.org/10.12681/jhvms.37150
Keywords:
efficacy ethnopharmacology liver fluke parasite control parasite resistance
V Chávez
Escuela de Posgrado, Universidad Nacional de Cajamarca, Av. Atahualpa 1050, 06003 Cajamarca, Perú, 2Facultad de Medicina Humana, Universidad Nacional de Cajamarca, Av. Atahualpa 1050, 06003 Cajamarca, Perú
L Vargas Rocha
Laboratorio de Parasitología Veterinaria y Enfermedades Parasitarias, Facultad de Ciencias Veterinarias, Universidad Nacional de Cajamarca, Av. Atahualpa 1050, 06003 Cajamarca, Perú
J Rojas-Moncada
Laboratorio de Parasitología Veterinaria y Enfermedades Parasitarias, Facultad de Ciencias Veterinarias, Universidad Nacional de Cajamarca, Av. Atahualpa 1050, 06003 Cajamarca, Perú
T Torrel
Laboratorio de Parasitología Veterinaria y Enfermedades Parasitarias, Facultad de Ciencias Veterinarias, Universidad Nacional de Cajamarca, Av. Atahualpa 1050, 06003 Cajamarca, Perú
Abstract

The Cajamarca Valley is an endemic area for Fasciola hepatica in cattle. Despite demonstrated resistance to triclabendazole, the use of this anthelmintic remains prevalent. Therefore, constant efficacy evaluations of this active ingredient are necessary, along with the exploration of alternatives for parasite control. This study aimed to determine the effectiveness of Amaro (Chuquiraga weberbaueri) and triclabendazole in controlling F. hepatica in artificially infected calves. Three groups (T0, T1, and T2) of four weaned Holstein calves each were formed and infected with 200 metacercariae. The control group (T0) received no antiparasitic treatment, group T1 was dosed with lyophilized aqueous extract of C. weberbaueri (200 mg/kg, orally, for four consecutive days), and group T2 received a single dose of triclabendazole (12 mg/kg, orally). Clinical efficacy was assessed using the Egg Count Reduction Test, and absolute efficacy was determined through necropsy. The average clinical efficacy and absolute efficacy of C. weberbaueri were 46.70% (95% CI: 33.27 – 60.13) and 58.63% (95% CI: 45.24 – 72.02), respectively. In contrast, triclabendazole demonstrated a clinical efficacy of 25.00% (95% CI: 12.62 – 37.38) and an absolute efficacy of 66.22% (95% CI: 66.22 – 79.08). Both treatments proved insufficiently active against Fasciola hepatica in calves, falling short of the category of effective fasciolicides. Nevertheless, using C. weberbaueri could be considered an environmentally friendly option to reduce the parasite burden in cattle.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Beesley NJ, Caminade C, Charlier J, Flynn RJ, Hodgkinson JE, Martinez-
Moreno A, et al (2018) Fasciola and fasciolosis in ruminants in
Europe: Identifying research needs. Transbound Emerg Dis 65: 199-
Benlarbi F, Mimoune N, Chaachouay N, Souttou K, Saidi R, Mokhtar
MR, et al (2023) Ethnobotanical survey of the traditional antiparasitic
use of medicinal plants in humans and animals in Laghouat (Southern
Algeria). Vet World 16: 357-68.
Cabada MM, López M, Cruz MCP, Delgado JR, Hill V, White AC (2016)
Treatment failure after multiple courses of triclabendazole among patients
with fascioliasis in Cusco, Peru: a case series. PLOS Negl Trop
Dis 10: e0004361.
Chávez A, Sánchez L, Arana C, Suárez F (2012) Resistance to anthelmintics
and prevalence of bovine fasciolosis in dairy farms in Jauja, Peru.
Rev Inv Vet Perú. 23: 90-7.
Dennis W R, Stone WM, Swanson LE (1954) A new laboratory and field
diagnostic test for fluke ova in feces. J Am Vet Med Assoc 124: 47-50.
Fissiha W, Kinde MZ (2021) Anthelmintic Resistance and Its Mechanism:
A Review. Infect Drug Resist 2021: 5403-10.
Githiori J, Höglund J, Waller P (2005) Ethnoveterinary plant preparations
as livestock dewormers: Practices, popular beliefs, pitfalls and prospects
for the future. Anim Health Res Rev 6: 91-103.
Goodenough AE, Webb JC, Yardley J (2019) Environmentally-realistic
concentrations of anthelmintic drugs affect survival and motility in
the cosmopolitan earthworm Lumbricus terrestris (Linnaeus, 1758).
Appl Soil Ecol 137: 87-95.
McMahon C, Edgar H, Hanna R, Ellison S, Flanagan AM, McCoy M A,
et al (2016) Liver fluke control on sheep farms in Northern Ireland:
A survey of changing management practices in relation to disease
prevalence and perceived triclabendazole resistance. Vet Parasitol
: 72-83.
Mpisana Z, Jaja IF, Byaruhanga C, Munyaradzi CM (2022) Body condition
scores, fluke intensity, liver pathology, and carcass quality of
different dairy cattle genotypes infected with Fasciola species at high
throughput abattoirs in South Africa. Parasitol Res 121: 1671-82.
Nasreldin N, Zaki RS (2020) Biochemical and immunological investigation
of fascioliasis in cattle in Egypt. Vet World 13: 923-30.
Nyahangare ET, Mvumi BM, Mutibvu T (2015) Ethnoveterinary plants
and practices used for ecto-parasite control in semi-arid smallholder
farming areas of Zimbabwe. J Ethnobiol Ethnomed 11: 30.
Ortiz P, Scarcella S, Cerna C, Rosales C, Cabrera M, Guzmán M, et al
(2013) Resistance of Fasciola hepatica against Triclabendazole in
cattle in Cajamarca (Peru): A clinical trial and an in vivo efficacy test
in sheep. Vet Parasitol 195: 118-21.
Ortiz P, Cerna C, García A, Hobán C, Murga-Moreno CA, Cabrera M, et
al (2023) Chemical Composition and Anthelmintic Activity of the Peruvian
Endemic Species Chuquiraga weberbaueri “Amaro” on Sheep
Fasciolosis. Rec Nat Prod 17: 1031-45.
Ramadan HK-A, Hassan WA, Elossily NA, Ahmad AA, Mohamed AA.,
Abd-Elkader AS, (2019) Evaluation of nitazoxanide treatment following
triclabendazole failure in an outbreak of human fascioliasis in
Upper Egypt. PLOS Negl Trop Dis 14: e0008443.
Rana MS, Lee SY, Kang HJ, Hur SJ (2019) Reducing Veterinary Drug
Residues in Animal Products: A Review. Food Sci Anim Resour 39:
-703.
Rázuri B, Murga-Moreno C, Vargas-Rocha L, Rojas-Moncada J, Torrel-
Pajares T (2023) First Report of Fasciola hepatica in Creole
Horses from the Northern Andes of Peru. Cienc Tecnol Agropecuaria
: e3190.
Rojas-Moncada J, Saldaña L, Urteaga V, Vergara R, Rojas A, Torrel S, et
al (2024) Efficacy of anthelmintic drugs to control Fasciola hepatica
in dairy cattle in Peru. Rev Colomb Cienc Pecu 37: 101-112.
Torrel S, Rojas-Moncada J, Saldaña K, Silva M, Gallardo I, Cadenillas
RP, et al (2023) Trematodes of dairy cattle grazing in Cajamarca:
Fasciola hepatica and Calicophoron microbothrioides. Rev Inv Vet
Perú 34: e24296.
Torrel TS, Rojas-Moncada J, Estela JE, Vargas-Rocha LA (2022) Fasciola
hepatica in Cavia porcellus from 10 communities in the district of
Chota, Cajamarca, Peru. Rev Inv Vet Perú 33: e20880.
Villar D, Schaeffer DJ (2022) Ivermectin use on pastured livestock in Colombia:
parasite resistance and impacts on the dung community. Rev
Colomb Cienc Pecu 36: 3-12.
Wood IB, Amaral NK, Bairden K, Duncan JL, Kassal T, Malone Jr JB,
et al (1955) World Association for the Advancement of Veterinary
Parasitology (W.A.A.V.P.) second edition of guidelines for evaluating
the efficacy of anthelmintics in ruminants (bovine, ovine, caprine).
Vet Parasitol 58: 181-213.
Young KE, Garza V, Snowden K, Dobson RJ, Powell D, Craig TM (1999)
Parasite diversity and anthelmintic resistance in two herds of horses.
Vet Parasitol 85: 205-14.
Zárate-Rendón DA, Briones-Montero A, Huaraca-Oré NA, Veirano GS,
Levecke B, Geldhof P (2023) Comparison of the therapeutic efficacy
of five anthelmintics against natural Fasciola hepatica infections in
dairy cattle from the Mantaro Valley, Peru. Vet Parasitol Reg Stud
Rep 38: 100827.
Most read articles by the same author(s)