The effect of vitamin E on mast cells in small intestine of broilers under heat stress

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Veröffentlicht: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15600
M. YILDIZ
Department of Occupational Health and Safety, School of Applied Sciences at Çan, University of Çanakkale Onsekiz Mart
S. KUM
Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Adnan Menderes
U. EREN
Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Adnan Menderes
Abstract

The aim of this study is to identify the effect of vitamin E (DL-α-tocopherol acetate) (300 IU/kg) on mast cells in the small intestine (duodenum, jejunum and ileum) under heat stress. In the study, 42 one-day-old Ross 308 male broiler chicks were used. The chicks were randomly separated into 3 groups as follows; control (22±2°C), heat stress (35°C, 5 hours/per day) and vitamin E (300 IU/kg/per day) + heat stress (35°C, 5 hours/per day). The applications of heat stress and vitamin E began on the fifteenth day and ended on the thirty-fifth day. Tissue samples were taken from animals in each group of four and five-week-old chickens. Tissue samples were fixed in BLA (Basic Lead Acetate) solution. The sections were stained with toluidine blue (TB) (pH 0.5) and alcian blue-critical electrolyte concentration (AB-CEC) (pH 5.8, 0.3 M MgCl2) / Safranin O (SO) (pH 1.0) combined method. It was determined that increasing of the exposure duration to heat stress increased the number of mast cells in the small intestine of the boilers. Also, it was revealed that vitamin E reduced mast cell population under heat stress. Consequently, heat stress may play a role in the pathogenesis of small intestine-associated with disorders and the supplementation of vitamin E can contribute to regulate small intestine functions of broilers by decreasing mast cell proliferation and activation under heat stress.

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Literaturhinweise
Ajith TA, Usha S, Nivitha V (2007) Ascorbic acid and α-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clin Chim Acta 375:82-86.
Aksoy and Cinar, 2008 The ontogeny, distribution and histochemistry of mast cells in the proventriculus of gallus gallus domestica in pre- and postnatal Periods. Van Vet J 19:25-29.
Au Yeung KJ, Smith A, Zhao A, Madden KB, Elfrey J, Sullivan C, Levander O, Urban JF, Shea-Donohue T (2005) Impact of vitamin E or selenium deficiency on nematode-induced alterations in murine intestinal function. Exp Parasitol 109:201-208.
Bingley PJ, Bonifacio E, Mueller PW (2003) Diabetes antibody standardization program: First assay proficiency evaluation. Diabetes 52:1128-1136.
Chen XY, Jia XM, Jia YS, Chen XR, Wang HZ, Qi WQ (2004) Development and distribution of mast cells and neuropeptides in human fetus duodenum. World J Gastroenterol 10:3349-3352.
Chowdhury VS, Tomonaga S, Ikegami T, Erwan E, Ito K, Cockrem JF, Furuse M (2014) Oxidative damage and brain concentrations of free amino acid in chicks exposed to high ambient temperature. Comp Biochem Physiol A Mol Integr Physiol 169:70-76.
Collins CB, McGrath J, Baird AW, Campion DP (2007) Effect of mast cell degranulation on chicken ileal ion transport in vitro. Poult Sci 86:843-849.
Coskun N, Indel SM, Elpek GO (2003) Mast cell density and neuronal hypertrophy in patients with acute appendicitis. Turk J Gastroenterol 14:54-58.
Deng W, Dong XF, Tong JM, Zhang Q (2012) The probiotic Bacillus licheniformis ameliorates heat stress- induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Poult Sci 91:575-582.
Eid Y, Ebeid T, Younis H (2006) Vitamin E supplementation reduces dexamethasone-induced oxidative stress in chicken semen. Br Poult Sci 47:350-356.
Enerbäck L (1966a) Mast cells in rat gastrointestinal mucosa. I. Effect of fixation. Acta Pathol Microbiol Scand 66:289-302.
Enerbäck L (1966b) Mast cells in rat gastrointestinal mucosa. 2. Dye-binding and metachromatic properties. Acta Pathol Microbiol Scand 66:303-312.
Eren U, Durukan A, Guzel N, Turkutanıt S, Erguldurenler S, Kara ME (2000) The mast cells in the dog intestinal mucosa. Ankara Univ Vet Fak Derg 47:125-134.
Goral V, Kucukoner M, Buyukbayram H (2010) Mast cells count and serum cytokine levels in patients with irritable bowel syndrome. Hepatogastroenterology 57:751-754.
Gueck T, Aschenbach JR, Fuhrmann H (2002) Influence of vitamin E on mast cell mediator release. Vet Dermatol 13:301-305.
Harem MK, Liman N (2009) Histochemical method for demonstrating quail mast cell types simultaneously. Biotech Histochem 84:275-282.
Henz BM, Maurer M, Lippert U, Worm M, Babina M (2001) Mast cells as initiators of immunity and host defense. Exp Dermatol 10:1-10.
Jakate S, Demeo M, John R, Tobin M, Keshavarzian A (2006) Mastocytic enterocolitis increased mucosal mast cells in chronic intractable diarrhea. Arch Pathol Lab Med 130:362-367.
Jacob C, Yang PC, Darmoul D, Amadesi S, Saito T, Cottrell GS, Coelho AM, Singh P, Grady EF, Perdue M, Bunnett NW (2005) Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and beta-arrestins. J Biol Chem 280:31936-31948.
Kabay SC, Ozden H, Guven G, Ustuner MC, Degirmenci I, Olgun E, Unal N (2009) Protective effects of vitamin E on central nervous system in streptozotocin-induced diabetic rats. Clin Invest Med 32:E314-321.
Karaca T, Yoruk M (2004) Morphological and histometrical study on distribution and heterogeneity of mast cells of chicken’s and quail’s digestive tract. Van Vet J 15:115-121.
Kempná P, Reiter E, Arock M, Azzi A, Zingg JM (2004) Inhibition of HMC-1 mast cell proliferation by vitamin E: involvement of the protein kinase B pathway. J Biol Chem 279:50700-50709.
Kum S, Eren U, Korkmaz D, Sandikci M, Aydemir I (2013) The effect of vitamin E on immunoglobulin-containing plasma cells in gutassociated lymphoid tissue (GALT) of broilers under heat stress. Vet Med Zoot 64:35-44.
Liu LL, He JH, Xie HB, Yang YS, Li JC, Zou Y (2014) Resveratrol induces antioxidant and heat shock protein mRNA expression in response to heat stress in black-boned chickens. Poult Sci 93:54-62.
Magro F, Fraga S, Azevedo I, Soares-da-Silva P (2006) Intestinal 5-hydroxytrptamine and mast cell infiltration in rat experimental colitis. Dig Dis Sci 51:495-501.
Mashaly MM, Hendricks GL, Kalama MA, Gehad AE, Abbas AO, Patterson PH (2004) Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult Sci 83:889-894.
Metcalfe DD, Baram D, Mekori YA (1997) Mast cells. Physiol Rev 77:1033-1079.
Moriguchi S (1998) The role of vitamin E in T-cell differentiation and the decrease of cellular immunity with aging. Biofactors 7:77-86.
Mujahid A, Pumford NR, Bottje W, Nakagawa K, Miyazawa T, Akiba Y, Toyomizu M (2007) Mitochondrial oxidative damage in chicken skeletal muscle induced by acute heat stress. J Poult Sci 44:439-445.
Ohta Y, Kobayashi T, Imai Y, Inui K, Yoshino J, Nakazawa S (2006) Effect of oral vitamin E administration on acute gastric mucosal lesion progression in rats treated with compound 48/80, a mast cell degranulator. Biol Pharm Bull 29:675-683.
Park JY, Hong SM, Klimstra DS, Goggins MG, Maitra A, Hruban RH (2011) Pdx1 expression in pancreatic precursor lesions and neoplasms. Appl Immunohistochem Mol Morphol 19:444-449.
Penissi A, Rudolph M, Piezzi R (2003) Role of mast cells in gastrointestinal mucosal defense. Biocell 27:163-172.
Puthpongsiriporn U, Scheideler SE, Sell JL, Beck MM (2001) Effects of vitamin E and C supplementation on performance, in vitro lymphocyte proliferation, and antioxidant status of laying hens during heat stress. Poult Sci 80:1190-1200.
Ramsay DB, Stephen S, Borum M, Voltaggio L, Doman DB (2010) Mast cells in gastrointestinal disease. Gastroenterol Hepatol 6:772-777.
Reiter E, Azzi A, Zingg JM (2007) Enhanced anti-proliferative effects of combinatorial treatment of delta-tocopherol and resveratrol in human HMC-1 cells. Biofactors 30:67-77.
Rengaraj D, Hong YH (2015) Effects of dietary vitamin E on fertility functions in poultry species. Int J Mol Sci 16:9910-9921.
Rocksén D, Ekstrand-Hammarström B, Johansson L, Bucht A (2003) Vitamin E reduces transendothelial migration of neutrophils and prevents lung injury in endotoxin-induced airway inflammation. Am J Respir Cell Mol Biol 28:199-207.
Scott JE, Dorling J (1965) Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions. Histochemie 5:221-223.
Shaik-Dasthagirisaheb YB, Varvara G, Murmura G, Saggini A, Caraffa A, Antinolfi P, Tete S, Tripodi D, Conti F, Cianchetti E, Toniato E, Rosati M, Speranza L,Pantalone A, Saggini R, Tei M, Speziali A, Conti P, Theoharides TC, Pandolfi F (2013) Role of vitamins D, E and C in immunity and inflammation. J Biol Regul Homeost Agents 27:291-295.
Singh UR, Malhotra A, Bhatia A (2008) Eosinophils, mast cells, nerves and ganglion cells in appendicitis. Indian J Surg 70:231-234.
Stasikowska-Kanicka O, Danilewicz M, Głowacka A, Wągrowska-Danilewicz M (2012) Mast cells and eosinophils are involved in activation of ulcerative colitis. Adv Med Sci 57:230-236.
Tsuduki T, Kuriyama K, Nakagawa K, Miyazawa T (2013) Tocotrienol (unsaturated vitamin E) suppresses degranulation of mast cells and reduces allergic dermatitis in mice. J Oleo Sci 62:825-834.
Uslu S, Yoruk M (2008) Morphological and histometrical studies on distribution and heterogeneity of mast cells in turkey digestive system. Van Vet J 19:47-51.
Wallert M, Schmölz L, Galli F, Birringer M, Lorkowski S (2014) Regulatory metabolites of vitamin E and their putative relevance for atherogenesis. Redox Biol 2:495-503.
Xiao G, Yuan F, Gu Z, Liu Z, Zhang Y, Su L (2013) Effect of heat stress on intestinal barrier function of human intestinal epithelial Caco-2 cells. Med J Chin PLA 38:472-475.
Zhao P, Dong L, Luo JY, Guan HT, Ma H, Wang XQ (2009) Changes of mast cells and gut hormones in rats with TNBS-induced ulcerative colitis. Nan Fang Yi Ke Da Xue Xue Bao 29:1359-1363.