Comparative study of hematological and blood chemistry of Persian sturgeon (Asipencer persicus) exposed to two common anticoagulants

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Veröffentlicht: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15609
H. GHOLIPOURKANANI
Department of Fisheries, Faculty of Agriculture and Natural Resource, Gonbad Kavous University
M. RANJDOOST
Department of Fisheries, Faculty of Agriculture and Natural Resource, Gonbad Kavous University
H. JAFARYAN
Department of Fisheries, Faculty of Agriculture and Natural Resource, Gonbad Kavous University
S. YELGHI
Iranian Fisheries Research Organization
Abstract

The effects of heparin and ethylenediaminetetraacetic acid (EDTA) on plasma biochemistry and hematological parameters in Asipencer persicus were studied. Differences in ALT and ALP activity were found between serum and heparinized plasma (P<0.05). AST activity in EDTA treated samples increased (P<0.05) compared to its values in serum. On the contrary, ALP activity showed a significant decrease compared to its serum level (P<0.05). Samples collected with EDTA showed an increase in haematocrit, mean corpuscular volume (MCV) and white blood cells (WBC) values (P<0.05) when compared to those collected in heparin. The other measured biochemical parameters did not show any variation. The present study suggests the use of heparin as a preferred anticoagulant for routine haematological analyses in the Persian sturgeon. However, both of these anticoagulants inflicted changes in selected biochemical parameters.

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Literaturhinweise
Blaxhall PC (1972) Haematological assessment of the health of freshwater fish. Journal of fish biology 4:593-604.
Blaxhall PC, Daisley KW (1973) Routine hematological methods for use with fish blood. J fish biol 5:771-781.
Blaxhall PC (1973) Error in haematocrit value produced by inadequate concentration of ethylenediamine tetra-acetate. J fish biol 5:767-769.
Burtis CA, Ashwood AR (2001) Tietz Fundamentals of Clinical Chemistry, fifth ed. WB Saunders, Philadephia, pp. 31–43.
Ceron J, Martınez-Subiela S, Hennemann C, Tecles F (2004) The effects of different inticoagulants on routine canine plasma biochemistry. The Veterinary Journal 167: 294–301.
Chaney AL, Merbach EP(1962) Modified reagents for determination of urea and ammonia. ClinChem 8:130-132.
Clark TD, Donaldson MR, Drenner SM, Hinch SG,Patterson DA, Hills V et al (2011) The efficacy of field techniques for obtaining and storing blood samples from fishes. J fish biol 79(5):1322-1333.
Dacie S, Lewis S (1991) Practical Haematology. Edn7, Churchill Livingstone, London, 1991.
Drabkin DL (1946) Spectrometric studies, XIV-the crystallographic and optimal properties of the hemoglobin of man in comparison with those of other species. J biol chem. 164(2):703-723.
Faggio C, Casella S, Arfuso F, Marafioti S, Piccione G, Fazio F (2012) Effect of storage time on haematological parameters in mullet, Mugil cephalus. Cell Biochem &Funct 31: 412-416. doi:10.1002/cbf.2915
Faggio C, Arfuso F, Piccione G, Zumbo A, Fazio F (2014) Effect of Three Different Anticoagulants and Storage Time on Haematological Parameters of Mugil cephalus (Linneaus, 1758). Turk J Fisheri &Aquat Scie 14: 615-621
Fischbach FT, Barnett M (2004) Dunning Williams & Wilkins, 2004 - Medical - 1291 pages Foster LB, Dunn RT (1973) Stable reagents for determination of serum triglycerides by a colorimetric Hantzsch condensation method. ClinChem 19: 338-340.
Fazlolahzadeh F, Keramati K, Nazifi S, Shirian S, Seifi S (2011) Effect of garlic (Allium sativum) on hematological parameters and plasma activities of ALT and AST of Rainbow trout in temperature stress. Aust J Basic Appl Sci 5 (9): 84-90.
Guder WG (2001) The quality of diagnostic samples. Blood Gas News 10:18–24.
Hattingh J (1975) Heparin and ethylenediamine tetra-acetate as anticoagulants for fish blood. Pflugers Arch 355:347-352.
Henry RJ (1974) Principles and Techniques. Clinical Chemistry, 2nd Ed. Harper and Row 1974:525.
Imanpour MR, Safari R, Asadi R (2012) The effects of anticoagulants on hematological indices of (Huso huso Linnaeus 1758) Animal research journal. 26 (4) 406-413.
Ishikawa MM, Pádua SBD, Satake F, Hisano H,Jerônimo GT, Martins ML et al. (2010) Heparin and Na 2 EDTA as anticoagulants for hybrid surubim catfish (Pseudoplatystoma reticulatum x P.corruscans): efficacy and hematological changes. Ciência Rural 40(7):1557-1561.
Jain NC (1993) Coagulation and its disorders; In: Essentials of veterinary hematology (ed.) NC Jain & Fabiger.Philadelphia 82-104.
Jones DG (1985b) Stability and storage characteristics of enzymes in sheep blood. Res in Vet Scie 38:307–311.
Korcock DE, Houston AH, Gray JD (1988) Effects of sampling conditions on selected blood variables of rainbow trout, Salmo gairdneri Richardson. J fish biol 33(2):319-330.
Lataretu A (2013) Comparative study of the effect of different anticoagulants on blood cell morphology in common carp (C. carpio) and rainbow trout (O. mykiss). Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca.
Anim Scie & Biotechnol 70(2):284-288.
Myers LJ, Pierce KR (1972) The effect of EDTA-anticoagulant on enzyme activity and pH of blood. Southwestern Veterinarian, 287–292.
Natt MP, Herrick CA (1952) New blood diluent for counting the erythrocytes and leucocytes of the chicken. Poultry Science 31(4):735-738.
Owen JA, Iggo B, Scandrett FJ, et al. (1954) The determination of creatinine in plasma or serum, and in urine: a critical examination. Biochem J 58: 426- 437.
Reitman S and Frankel S (1957) A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin 1957; 28:56-63.
Sala-Rabanal M, Sánchez J, Ibarz A, Fernández-Borràs J, Blasco J, Gallardo MA et al. (2003) Effects of low temperatures and fasting on hematology and plasma composition of gilthead sea bream (Sparus aurata). Fish Physiol & Biochem 29:105-115.
Smit GL, Hattingh J, Schoonbee HJ (1977) Observations on some effects of disodium ethylenediamine tetra-acetate and heparin on fish blood. Comp Biochem & Physiol Part C: Comp Pharmacol 57(1):35-38.
Svobodova Z, Pravda D, Palackova J (1991) Unified methods of haematological examination of fish. Research Institute of fish culture and hydrobiology 22:31.
Trinder P (1969) A colorimetric method for the determination of glucose. Annlin Biochem 6:24-26.
Walencik J, Witeska M (2007) The effects of anticoagulants on hematological indices and blood cell morphology of common carp (Cyprinus carpio L.). Comparative Biochemistry and Physiology Part C: Toxicol & Pharmacol 146(3):331-335.
Weatherby D, Scott F (2002) Blood Chemistry and CBC Analysis: Clinical Laboratory Testing from a Functional Perspective, Bear Mountain.
Witeska M, Wargocka W (2011) Disodium EDTA used as anticoagulant causes hemolysis in common carp blood. Turk J Vet & Anim Scie 35(2):99-104.
Zlatki A, Zak B, Boyle AJ (1953) A new method for the direct determination of serum cholesterol. J Lab Clin Med 45: 486.