Genomic identification of Toxic shock syndrome producing and methicillin resistant Staphylococcus aureus strains in human and sheep isolates
Abstract
Disease-associated Staphylococcus aureus strains often promote infections by producing potent protein toxins such as toxic shock syndrome toxin (TSST). The mecA gene allows a bacterium to be resistant to antibiotics such as methicillin, penicillin and other penicillin-like antibiotics. The aim of this study was to determine the prevalence of Staphylococcus aureus strains producing these two genes. In this study, within 110 cases isolated in Chaharmahal and Bakhtiari province, Staphylococcus aureus was isolated by microbiological methods. Then PCR was done for 66 samples to identify the mecA and TSST-1 genes. The results showed within 30 samples of human skin infection 18 cases (60%) were MRSA and 5 samples (16.66%) were positive for TSST-1 gene. Within 36 samples of ewe subacute mastitis 10 samples (27.77%) and 5 (13.88%) had mecA and TSST-1 genes respectively. Therefore the prevalence of methicillin resistance and toxic shock syndrome producing Staphylococcus aureus isolates was significant in Chaharmahal and Bakhtiari. Due to the presence of these isolates in Iran and their threatening role in public health, more attention for their monitoring and treatment is essential.
Article Details
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MOKHTARI, A., EBRAHIMI KAHRIZSANGI, A., & HASANI, P. (2018). Genomic identification of Toxic shock syndrome producing and methicillin resistant Staphylococcus aureus strains in human and sheep isolates. Journal of the Hellenic Veterinary Medical Society, 69(2), 941–950. https://doi.org/10.12681/jhvms.18016
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- Bd. 69 Nr. 2 (2018)
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- Research Articles
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Literaturhinweise
Aires-de-Sousa M, Parente CESR, Vieira-da-Mota OV et al. (2007) Characterization of Staphylococcus aureus isolates from buffalo, bovine, ovine, and caprine milk samples collected in Rio de Janeiro State, Brazil. Appl Environ Microbiol 73:3845-3849.
AL-Ruaily MA, Khalil OM (2011) Detection of mecA gene in methicillin-resistant Staphylococcus aureus (MRSA) at prince A/Rhman, Sidery Hospital , AL-Jouf , Saudi Arabia. J Med Genet Genomics 3(3):41-45.
Arfatahery N, Davoodabadi A, Abedimohtasab T (2016) Characterization of toxin genes and antimicrobial susceptibility of Staphylococcus aureus isolates in fishery products in Iran. Sci Rep 6: 34216.
Balaban N, Rasooly A (2000) Staphylococcal enterotoxin. Int J Food Microbiol 61:1-10.
Becker K, Friedrich AW, Lubritz G, Weilert M, Peters G, Von Eiff C (2003) Prevalence of genes encoding pyrogenic toxin superantigens and exfoliative toxins among strains of Staphylococcus aureus isolated from blood and nasal specimens. J Clin Microbiol 4:139-143.
Chapaval L, Moon DH, Gomes JE, Duarte FR, Tsai SM (2008) An alternative method for Staphylococcus aureus DNA isolation. Arq Bras Med Vet Zootec 60(2):299-306.
Cole AM, Tahk S, Oren A, Yoshioka D, Kim YH, Park A, Ganz T (2001) Determinants of Staphylococcus aureus nasal carriage. Clin Diagn Lab Immunol 8(6):1064–1069.
de Carvalho MJ, Pimenta FC, Hayashida M, Gir E, da Silva AM, Barbosa CP, Canini SR, Santiago S ( 2009) Prevalence of methicillin-resistant and methicillin-susceptible S. aureus in the saliva of health professionals. Clinics (Sao Paulo) 64(4):295-302.
Dastmalchi Saei h, Ahmadi M, Farahmand-Azar S, Anassori E (2013) Identification of Toxic Shock Syndrome Toxin-1 (TSST-1) gene in Staphylococcus aureus isolated from bovine mastitis milk. Arch Razi Inst 68(1):17-22.
Dinges MM, Paul MO, Patrick M (2000) Exotoxins of Staphylococcus aureus. Clin Microbiol Rev 13(1):16-34.
Deurenberg RH, Nieuwenhuis RF, Driessen C, London N, Stassen FR, van Tiel FH, Stobberingh EE, Vink C (2005) The prevalence of the Staphylococcus aureus tst gene among community- and hospital acquired strains and isolates from Wegener’s Granulomatosis patients. FEMS Microbiol Lett 245:185-189.
Enright MC, Robinson DA, Randle G, Feil EJ, Grundmann H, Spratt BG (2002) The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc Natl Acad Sci 99:7687-7692.
Ertas N, Gonulalan Z, Yildirim Y, Kum E (2001) Detection of Staphylococcus aureus Enterotoxins in sheep cheese and Dairy Desserts by Multiplex PCR Technique. Int J Food Microbiol 142(1-2):74-70.
Gilbert RJ, Humphrey TJ (1998) Food-borne bacterial gastroenteritis. In: Topley and Wilson’s microbiology and microbial infections. 3rd ed, A Hodder Arnold Publication, London: pp 577-600.
Goud R, Gupta S, Neogi U, Agarwal D, Naidu K, Chalannavar R, Subhaschandra G ( 2011) Community prevalence of methicillin and vancomycin resistant Staphylococcus aureus in and around Bangalore, southern India. Rev Soc Bras Med Trop 44:309-312.
Haley RW, Hightower AW, Khabbaz RF, Thorsberry C, Martone WJ, Allen JR, Hughes JM (1982) The emergence of methicillin-resistant Staphylococcus aureus infections in United States hospitals. Ann Intern Med 97:297–308.
Hartman BJ, Tomasz A (1984) Low-affinity binding protein associated with beta-lactam resistance in Staphylococcus aureus. J Bacteriol 158:513–516.
Hoseini Alfatemi SM, Motamedifar M, Hadi N, Sedigh Ebrahim Saraie H (2014) Analysis of Virulence Genes Among Methicillin Resistant Staphylococcus aureus (MRSA) Strains. Jundishapur J Microbiol 7(6): e10741.
Hososaka Y, Hanaki H, Endo H, Suzuki Y, Nagasava Z (2007) Characterization of Oxacillin-susceptible mecA- positive Staphylococcus aureus: A new type of MRSA. J Infect Chemother 13:79-86.
Iandolo JJ (1989) Genetic analysis of extracellular toxins of Staphylococcus aureus. Annu Rev Microbiol 43:375–402.
Japoni A, Alborzi A, Orafa F, Rasouli M, Farshad S (2004) Distribution Patterns of Methicillin Resistance Gene (mecA) in Staphylococcus aureus isolated from clinical Specimens. Iran Biomed J 8(4):173-178.
Johnson WM, Tyler SD, Ewan EP, Ashton FE, Pollard DR, Rozee KR (1991) Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction. J Clin Microbiol 29:426–430.
Khoei F, Mobaiyen H, Nahaei MR, Sadeghi Mohammadi S (2014) Antibiotic resistance pattern and frequency of mecA gene in Staphylococcus aureus isolated from Shohada hospital, Tabriz. J Med Microbiol Infec Dis 2(3):105-108.
Kluytmans J, van Belkum A, Verbrugh H, Van Belkum V (1997) Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin M,icrobiol Rev 10(3):505–520.
Low DE (2013) Toxic shock syndrome: major advances in pathogenesis, but not treatment. Crit Care Clin 29 (3): 651–75.
McDougall S, Murdough P, Pankey W et al. (2001) Relationship among somatic cell count, California mastitis test, impedance and bacteriological status of milk in goats and sheep in early lactation. Small Ruminant Res 40: 245-254.
Mehrotra M, Wang G, Johnson WM (1996) Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxin, exfoliative toxin, toxic shock syndrome: Role of the environment, the host and the microorganism. J Biomed Sci 53(4):284-289.
Nemati M, Shamsi M (2015) Detection of mecA, eta, etb, and tst-1 genes from staphylococcal isolates. J Bas Res Med Sci 2(2):13-17.
Najera-sanchez G, Maldonado-Rodriguez R, Ruíz Olvera P, de la Garza LM (2003) Development of two multiplex polymerase chain reactions for the detection of enterotoxigenic strains of Staphylococcus aureus isolated from foods. J Food Prot 66:1055-1062.
Orwin PM, Fitzgerald JR, Leung DYM, Gutierrez JA, Bohach GA, Schlievert PM (2003) Characterization of Staphylococcus aureus enterotoxin L. Infect Immun 71:2916-2919.
Parsonnet J, Goering RV, Hansmann MA, Jones MB, Ohtagaki K, Davis CC, Totsuka K (2008) Prevalence of Toxic Shock Syndrome Toxin 1 (TSST-1)-Producing Strains of Staphylococcus aureus and Antibody to TSST-1 among Healthy Japanese Women. J Clin Microbiol 46(8):2731–2738.
Pesavento G, Ducci B, Comodo N Lonostro A (2007) Antimicrobial resistance profile of Staphylococcus aureus isolated from raw milk: A research for methicillin resistant Staphylococcus aureus (MRSA). Food control 18:196-200.
Sajith Khan AK, Shetty PJ, Sarayu Y L, Chidambaram A, Ranganathan R (2012) Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. Int J Health Rehabil Sci 1(2):64-68.
Salisbury SM, Sabatini LM, Spiegel CA (1997) Identification of methicillin-resistant staphylococci by multiplex polymerase chain reaction assay. Am J Clin Pathol 107:368–373.
Schalm OW, Noorlander DO (1957) Experiments and observations leading to development of the CaliforniaMastitis Test. J Am Vet Med Assoc 30:199-207.
Schmitz FJ, Mackenzie CR, Hofmann B, Verhoef J, Finken-Eigen M, Heinz HP, Kohrer K (1997) Specific information concerning taxonomy, pathogenicity, and methicillin resistance of staphylococci obtained by a multiplex PCR. J Med Microbiol 46:773–778.
Schlievert PM (1993) Role of superantigens in human diseases. J Infect Dis 167:997–1002.
Sung JM1, Lloyd DH, Lindsay JA (2008) Staphylococcus aureus host specificity: comparative genomics of human versus animal isolates by multi-strain microarray. Microbiology. 154(7):1949-59.
Tuncer I, Kalem F, Cosar M, Arsalan U (2009) Antibiotic susceptibility of Staphylococcus aureus strains isolated from blood stream infections. Turk Mikrobiyol Cemiy Derg 39(1-2):20-22.
Utsui Y, Yokota T (1985) Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus. Antimicrob Agents Chemother 28:397–403.
Vannuffel P, Gigi J, Ezzedine H, Vandercam B, Delmee M, Wauters G, Gala J (1995) Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J Clin Microbiol 33:2864–2867.
Varmazyar-najafi M, Pajohi –alamoti MR, MohammadzadehA, Mahmoodi P (2016) Detection of methicillin-resistance gene in Staphylococcus aureus isolated from traditional white cheese in Iran. Arch hyg Sci 5(4): 302-309.
Zamani A, Sadeghian S, Ghaderkhani J, Alikhani MY, Najafimosleh M, Taghi Goodarzi M, Shahrabi Farahani H, Yousefi-Mashouf R(2007) Detection of methicillin-resistance (mec-A) gene in Staphylococcus aureus strains by PCR and determination of antibiotic susceptibility. Annu Rev Microbiol 57(2):273-276.
Zambardi G, Reverdy ME, Bland S, Bes M, Freney J, Fleurette J (1994) Laboratory diagnosis of oxacillin resistance in Staphylococcus aureus by a multiplex-polymerase chain reaction assay. Diagn Microbiol Infect Dis 19:25–31.
Zeinali E, Moniri R, Safari M, Mousavi GA (2011) Molecular characterization and SCC mec typing in methicillin- resistant Staphylococcus aureus isolated from clinical samples. Feyz J KAMUS 14(4): 439-44.
