Effects of Indigenous Spore-Forming Probiotic as Feed Supplement on Performance and Safety in Broilers

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DOI: https://doi.org/10.12681/jhvms.22234
N.G. HOSSEINI
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
M.H. MODARRESSI
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
S.N. MOUSAVI
Departments of Animal Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
M.T. EBRAHIMI
Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract

Probiotics colonize the intestine of animals and birds and provide useful effects on their performance and immune status. This study describes a high throughput screening and characterization of spore-forming bacteria from Iranian poultry farms with the aim to identify potential probiotic native Bacillus spp. and determine its effects on growth performance, hemato-biochemical parameters, immunity, intestinal microflora, morphology and MUC2 gene expression of broiler chickens. A total of 300 one-day-old female Ross 308 broilers (42.6 ± 0.6 g) were used in a 6-wk study. Broilers were randomly allotted to 1 of 3 dietary treatments consisting of 4 replicate cages with 25 broilers each: 1- Control (Corn-soy-based diet: C), 2- C + 200 g/ton of the GalliPro® (Bacillus subtilis DSM 17299, 4×109 CFU/g, as positive control group: PC), 3- C + 200 g/ton of the native probiotic (B. tequilensis K03, 4×109 CFU/g: NP) identified in this study. During the experiment parameters were measured weekly. The results revealed that birds of the NP and PC groups exhibited improved feed conversion ratio (FCR) and increased body weight (BW), carcass and breast meat yield compared with the birds of the C group (P<0.05). Also, lymphocytes level, antibody titers against Newcastle diseases virus (NDV) and infectious bronchitis virus (IBV) of vaccinated birds were increased, while serum triglycerides, total cholesterol levels and abdominal fat of birds fed NP and PC were decreased compared to birds of the C group (P<0.05). The villus height, the relative expression of MUC2 gene and Bacillus spp. populations were increased, while E. coli was significantly decreased in the ileum content of treated groups (P<0.05). These results indicate that the identified native B.tequilensis K03 strain can improve immunity and broiler performance by modifying intestinal microflora and morphology. Studied native probiotic Bacillus tequilensis K03 has useful effects on health status and it can be used as poultry feed supplement.

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Literaturhinweise
Afsharmanesh M, Sadaghi B, Silversides FG (2011) Influence of supplementation of prebiotic, probiotic, and antibiotic to wet-fed wheatbased diets on growth, ileal nutrient digestibility, blood parameters, and gastrointestinal characteristics of broiler chickens. Comp Clin Path 22:245-251.
Amerah AM, Quiles A, Medel P, Sánchez J, Lehtinen MJ, Gracia MI (2013) Effect of pelleting temperature and probiotic supplementation on growth performance and immune function of broilers fed maize/soy-based diets. Anim Feed Sci Tech 180:55-63.
Belardelli FI (1995) Role of interferons and other cytokines in the regulation of the immune response. Apmis 103:161-179.
Beski SSM, Al-Sardary SYT (2015) Effects of dietary supplementation of probiotic and synbiotic on broiler chickens hematology and intestinal integrity. Int J Poult Sci 14:31-36.
Coates ME, Fuller R, Harrison GF, Lev M, Suffolk SF (1963) A comparision of the growth of chicks in the Gustafsson germ-free apparatus and in a conventional environment, with and without dietary supplements of penicillin. Br J Nutr 17:141-150.
Cutting SM (2011) Bacillus probiotics. Food Microbiol 28:214-220.
De Smet I, De Boever P, Verstraete W (1998) Cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity. Br J Nutr 79:185-194.
Deng W, Dong XF, Tong JM, Zhang Q (2012) The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology and intestinal mucosal immunity in laying hens. Poult Sci 91:575-582.
Deniz G, Orman A, Cetinkaya F, Gencoglu H, Meral Y, Turkmen II (2011) Effects of probiotic (Bacillus subtilis DSM 17299) supplementation on the caecal microflora and performance in broiler chickens. Revue Med Vet 11:538-545.
Elshaghabee FMF, Rokana N, Gulhane RD, Sharma C, Panwar H (2017) Bacillus as potential probiotics: status, concerns, and future perspec tives. Front Microbiol 8:1490.
Ferket PR, Gernat AG (2006) Factors that affect feed intake of meat birds: A review. Int J Poult Sci 5:905-911.
Forder REA, Howarth GS, Tivey DR, Hughes RJ (2007) Bacterial modulation of small intestinal goblet cells and mucin composition during early potshatch development of poultry. Poult Sci 86:2396-2403.
Forder RE, Nattrass GS, Geier MS, Hughes RJ, Hynd PI (2012) Quantitative analyses of genes associated with mucin synthesis of broiler chickens with induced necrotic enteritis. Poult Sci 91:1335-1341.
Gatson JW, Benz BF, Chandrasekaran C, Satomi M, Venkateswaran K, Hart ME (2006) Bacillus tequilensis sp. nov., isolated from a 2000-year-old mexican shaft-tomb, is closely related to Bacillus subtilis. Int J Syst Evol Microbiol 56:1475-1484.
Ghadban GS (2002) Probiotics in broiler production- a review. Arch Geflugelkd 66:49-58.
Ghorban Hosseini N, Modarressi MH, Mousavi SN, Tajabadi Ebrahimi M (2019) Evaluation of novel probiotic Bacillus strains based on enzyme production and protective activity against salmonellosis. J HELL VET MED SOC 69:1205-1212.
Guyard-Nicodeme M, Keita A, Quesne S, Amelot M, Poezevara T, Le Berre B, Sánchez J, Vesseur P, Martín Á, Medel P, Chemaly M (2016) Efficacy of feed additives against Campylobacter in live broilers during the entire rearing period. Poult Sci 95:298-305.
Hatab MH, Elsayed MA, Ibrahim NS (2016) Effect of some biological supplementation on productive performance, physiological and immunological response of layer chicks. J Radiat Res Appl Sci 9:185-192.
Hong HA, Huang JM, Khaneja R, Hiep LV, Urdaci MC, Cutting SM (2008) The safety of Bacillus subtilis and Bacillus indicus as food probiotics. J Appl Microbiol Biochem 105:510-520.
Huang MK, Choi YJ, Houde R, Lee JW, Lee B, Zhao X (2004) Effects of Lactobacilli and an acidophilic fungus on the production performance and immune responses in broiler chickens. Poul Sci 83:788-795.
Ichikawa H, Kuroiwa T, Inagaki A, Shineha R, Nishihira T, Satomi S, Sakata T (1999) Probiotic bacteria stimulate gut epithelial cell proliferation in rat. Dig Dis Sci 44:2119-2123.
Iji PA, Saki AA, Tivey DR (2001) Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides. Anim Feed Sci Tech 89:175-188.
Jeevana Lakshmi P, Kumari Chitturi CM, Lakshmi VV (2013) Efficient degradation of feather by keratinase producing Bacillus sp. Int J Microbiol 7.
Jeong JS, Kim IH (2014) Effect of Bacillus subtilis C-3102 spores as aprobiotic feed supplement on growth performance, noxious gas emission, and intestinal microflora in broilers. Poul Sci 93:3097-3103.
Kabir SM (2009) The role of probiotics in the poultry industry. Int J Mol Sci 10:3531-3546.
Khaksefidi A, Ghoorchi T (2006) Effect of probiotic on performance and immunocompetence in broiler chicks. Poul Sci 43:296-300.
Knap I, Kehlet AB, Bennedsen M, Mathis GF, Hofacre CL, Lumpkins BS, Jensen MM, Raun M, Lay A (2011) Bacillus subtilis (DSM17299) significantly reduces Salmonella in broilers. Poul Sci 90:1690-1694.
Latorre JD, Hernandez-Velasco X, Wolfenden RE, Vicente JL, Wolfenden AD, Menconi A, Bielke LR, Hargis BM. Tellez G (2016) Evaluation and selection of Bacillus species based on enzyme production, antimicrobial activity, and biofilm synthesis as direct-fed microbial candidates for poultry. Front Vet Sci 3:95.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2- ΔΔCt method. Methods 25:402-408.
Mattar A, Teitelbaum DH, Drongowski R, Yongyi F, Harmon C, Coran A (2002) Probiotics up-regulate MUC-2 mucin gene expression in a Caco-2 cell-culture model. Pediatr Surg Int 18:586-590.
Melegy T, Khaled NF, El-Bana R, Abdellatif H (2011) Effect of dietarysupplementation of Bacillus subtilis PB6 (Clostat TM) on performance, immunity, gut health and carcass traits in broilers. AM SCI 7:891-898.
Mingmongkolchai S, Panbangred W (2018) Bacillus probiotics: an alternative to antibiotics for livestock production. J Appl Microbiol Biochem 124:1334-1346.
Mountzouris KC, Tsirtsikos P, Kalamara E, Nitsch S, Schatzmayr G, Fegeros K (2007) Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poul Sci 86:309-317.
Nawab A, Liu W, Li G, Ibtisham F, Fox DP, Zhao Y, Wo J, Xiao M, NawabY, An L (2018) The potential role of probiotics (nutraceuticals) in gut health of domestic animals; an alternative to antibiotic growth promoters. J HELL VET MED SOC 69:1169-1188.
Neveling DP, Van Emmenes L, Ahire JJ, Pieterse E, Smith C, Dicks LMT(2017) Safety assessment of antibiotic and probiotic feed additives for Gallus gallus domesticus. Sci Rep 7:12767.
Opalinski M, Maiorka A, Dahlke F, Cunha F, Vargas FSC, CardozoE (2007) On the use of a probiotic (Bacillus subtilis - strain DSM 17299) as growth promoter in broiler diets. Braz J Poultry Sci 9:99-103.
Park JH, Kim IH (2014) Supplemental effect of probiotic Bacillus subtilis B2A on productivity, organ weight, intestinal Salmonella microflora, and breast meat quality of growing broiler chicks. Poul Sci 93:2054-2059.
Rahmani HR, Speer W, Modirsanei M (2005) The Effect of Intestinal Ph on Broiler Performance and Immunity. In: The 15th European Symposium on poultry nutrition, Balatonfüred, Hungary: pp 338-340.
Reis MP, Fassani EJ, Júnior AG, Rodrigues PB, Bertechini AG, Barrett N, Persia ME, Schmidt CJ (2017) Effect of Bacillus subtilis (DSM 17299) on performance, digestibility, intestine morphology, and pH in broiler chickens. J Appl Poult Res 26:573-583.
Rowghani E, Arab M, Akbarian A (2007) Effects of a probiotic and other feed additives on performance and immune response of broiler chicks. Int J Poult Sci 6:261-265.
Santoso U, Tanaka K, Ohtani S, Sakaida M (2001) Effect of fermented product from Bacillus subtilis on feed conversion efficiency, lipid accumulation and ammonia production in broiler chicks. Asian-Australas J Anim Sci 14:333-337.
Sen S, Ingale SL, Kim YW, Kim JS, Kim KH, Lohakare JD, Kim EK, Kim HS, Ryu MH, Kwon IK, Chae BJ (2012) Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Res Vet Sci 93:264-268.
Shokryazdan P, Jahromi MF, Liang JB, Ramasamy K, Sieo CC, Ho YW (2017) Effects of a Lactobacillus salivarius mixture on performance, intestinal health and serum lipids of broiler chickens. PLoS one 12:e0175959.
Tellez G, Pixley C, Wolfenden RE, Layton SL, Hargis BM (2012) Probiotics/direct fed microbials for Salmonella control in poultry. Food Res Int 45:628-633.
Thirabunyanon M, Thongwittaya N (2012) Protection activity of a novel probiotic strain of Bacillus subtilis against Salmonella enteritidis infection. Res Vet Sci 93:74-81.
Wu BQ, Zhang T, Guo LQ, Lin JF (2011) Effects of Bacillus subtilis KD1 on broiler intestinal flora. Poult Sci 90:493-2499.
Xu CL, Ji C, Ma Q, Hao K, Jin ZY, Li K (2006) Effects of a dried Bacillus subtilis culture on egg quality. Poult Sci 85:364-368.
Yang J, Qian K, Zhang W, Xu Y, Wu Y (2016) Effects of chromium-enriched Bacillus subtilis KT260179 supplementation on chicken growth performance, plasma lipid parameters, tissue chromium levels, cecal bacterial composition and breast meat quality. Lipids Health Dis 15:188.
Zaghari M, Zahroojian N, Riahi M, Parhizkar S (2015) Effect of Bacillus subtilis spore (GalliPro®) nutrients equivalency value on broiler chicken performance. Ital J Anim Sci 14:3555.